Restriction map for
pFN21AB9869
(Circular) (Six-base) MAPSORT of: pFN21AB9869.seq Check: 687 from: 1 to: 6085
pFN21AB9869 6085 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3072 3895 278
Size: 53 83 186 936 1536 823 2468
Fragments arranged by size:
2468 1536 936 823 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2646 3428 1937
Size: 709 782 4594
Fragments arranged by size:
4594 782 709
Acc65I G'GTAC_C
Cuts at: 3406 3406
Size: 6085
AclI AA'CG_TT
Cuts at: 1563 4212 4585 1563
Size: 2649 373 3063
Fragments arranged by size:
3063 2649 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2061 2096 4148 5532 5800 1632
Size: 429 35 2052 1384 268 1917
Fragments arranged by size:
2052 1917 1384 429 268 35
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5888
AflIII A'CryG_T
Cuts at: 1203 4984 5961 1203
Size: 3781 977 1327
Fragments arranged by size:
3781 1327 977
AgeI A'CCGG_T
Cuts at: 4916 4916
Size: 6085
AhdI GACnn_n'nnGTC
Cuts at: 4814 4814
Size: 6085
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6085
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2479 2846 1184
Size: 1295 367 4423
Fragments arranged by size:
4423 1295 367
AlwNI CAG_nnn'CTG
Cuts at: 2086 2498 2966 3828 5400 2086
Size: 412 468 862 1572 2771
Fragments arranged by size:
2771 1572 862 468 412
ApaI G_GGCC'C
Cuts at: 2810 2810
Size: 6085
ApaLI G'TGCA_C
Cuts at: 2225 4142 5298 2225
Size: 1917 1156 3012
Fragments arranged by size:
3012 1917 1156
ApoI r'AATT_y
Cuts at: 1493 2068 3394 3620 3772 5881 1493
Size: 575 1326 226 152 2109 1697
Fragments arranged by size:
2109 1697 1326 575 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4639 160
Size: 872 3607 1606
Fragments arranged by size:
3607 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
AvaI C'yCGr_G
Cuts at: 1870 1943 2013 2172 2213 3410 1870
Size: 73 70 159 41 1197 4545
Fragments arranged by size:
4545 1197 159 73 70 41
BamHI G'GATC_C
Cuts at: 3415 3782 3415
Size: 367 5718
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3406 4861 618
Size: 325 217 627 1619 1455 1842
Fragments arranged by size:
1842 1619 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2283 2810 2999 3404 1391
Size: 892 527 189 405 4072
Fragments arranged by size:
4072 892 527 405 189
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5114
BbvCI CC'TCA_GC
Cuts at: 2959 2959
Size: 6085
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4276 4310 1903
Size: 34 2339 34 3678
Fragments arranged by size:
3678 2339 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3977 5187 3977
Size: 1210 4875
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6085
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2128 2331 2423
Size: 107 122 71 631 605 456 203 92
Cuts at: 2423 4696 136
Size: 2273 1525
Fragments arranged by size:
2273 1525 631 605 456 203 122 107
92 71
BglII A'GATC_T
Cuts at: 6080 6080
Size: 6085
BlpI GC'TnA_GC
Cuts at: 2106 3502 2106
Size: 1396 4689
Bme1580I G_kGCm'C
Cuts at: 1790 2229 2556 2810 3203 4146 5302 1790
Size: 439 327 254 393 943 1156 2573
Fragments arranged by size:
2573 1156 943 439 393 327 254
BmgBI CAC'GTC
Cuts at: 1318 2350 1318
Size: 1032 5053
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3254 3816 4774
Size: 416 53 83 399 24 1837 562 958
Cuts at: 4774 442
Size: 1753
Fragments arranged by size:
1837 1753 958 562 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3263 4745 1239
Size: 2024 1482 2579
Fragments arranged by size:
2579 2024 1482
Bpu10I CC'TnA_GC
Cuts at: 2959 2959
Size: 6085
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3282 4210 5075 5373 3282
Size: 928 865 298 3994
Fragments arranged by size:
3994 928 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4748 882
Size: 617 3249 2219
Fragments arranged by size:
3249 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3876 493
Size: 711 2672 2702
Fragments arranged by size:
2702 2672 711
BsaBI GATnn'nnATC
Cuts at: 6079 6079
Size: 6085
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2090 3069 3892 4274
Size: 53 83 186 936 557 979 823 382
Cuts at: 4274 4909 275
Size: 635 1451
Fragments arranged by size:
1451 979 936 823 635 557 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2137 4518 4916 5190 5337 5748 2137
Size: 2381 398 274 147 411 2474
Fragments arranged by size:
2474 2381 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5860
Fragments arranged by size:
5860 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2072 2947 3017 1186
Size: 847 39 875 70 4254
Fragments arranged by size:
4254 875 847 70 39
BseYI C'CCAG_C
Cuts at: 2218 2698 5288 5784 2218
Size: 480 2590 496 2519
Fragments arranged by size:
2590 2519 496 480
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2916 1163
Size: 573 1180 4332
Fragments arranged by size:
4332 1180 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2316 2427 3535 4296 5324 1540
Size: 275 186 315 111 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 315 275 186 111
BsiHKAI G_wGCw'C
Cuts at: 2229 2739 4146 4231 5302 2229
Size: 510 1407 85 1071 3012
Fragments arranged by size:
3012 1407 1071 510 85
BsmI GAATG_Cn'
Cuts at: 2468 3050 3596 3689 2468
Size: 582 546 93 4864
Fragments arranged by size:
4864 582 546 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2283 2556 2739 2810 2999 3203
Size: 399 439 54 273 183 71 189 204
Cuts at: 3203 3404 4146 4231 5302 1391
Size: 201 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 439 399 273 204 201
189 183 85 71 54
BspEI T'CCGG_A
Cuts at: 5748 5748
Size: 6085
BspHI T'CATG_A
Cuts at: 3974 5704 3974
Size: 1730 4355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3440 844
Size: 2596 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2165 2862 2979 3972 1394
Size: 429 342 697 117 993 3507
Fragments arranged by size:
3507 993 697 429 342 117
BsrDI GCAATG_nn'
Cuts at: 65 1248 4580 4754 65
Size: 1183 3332 174 1396
Fragments arranged by size:
3332 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2196 2315 4729 4916 1700
Size: 110 386 119 2414 187 2869
Fragments arranged by size:
2869 2414 386 187 119 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6085
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6085
BssSI C'ACGA_G
Cuts at: 2603 4145 5157 2603
Size: 1542 1012 3531
Fragments arranged by size:
3531 1542 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2206 794
Size: 1412 4673
BstBI TT'CG_AA
Cuts at: 4889 4889
Size: 6085
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6085
BstYI r'GATC_y
Cuts at: 1972 3273 3415 3782 4167 4184 5636 5722 5734
Size: 1301 142 367 385 17 1452 86 12
Cuts at: 5734 6080 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1301 385 367 346 142 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3078 3078
Size: 6085
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4594
Fragments arranged by size:
4594 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5346
Fragments arranged by size:
5346 683 56
BtsI GCAGTG_nn'
Cuts at: 2463 2496 3601 4395 4415 2463
Size: 33 1105 794 20 4133
Fragments arranged by size:
4133 1105 794 33 20
ClaI AT'CG_AT
Cuts at: 1554 3363 1554
Size: 1809 4276
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6050
DraI TTT'AAA
Cuts at: 3389 3389
Size: 6085
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5092 809
Size: 507 3776 1802
Fragments arranged by size:
3776 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2313 2404 2424
Size: 54 1425 50 275 18 483 91 20
Cuts at: 2424 3532 4421 6050 8
Size: 1108 889 1629 43
Fragments arranged by size:
1629 1425 1108 889 483 275 91 54
50 43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 2313 2424 3532 1537
Size: 275 501 111 1108 4090
Fragments arranged by size:
4090 1108 501 275 111
EarI CTCTTCn'nnn_
Cuts at: 1353 3183 4015 1353
Size: 1830 832 3423
Fragments arranged by size:
3423 1830 832
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2504 4672 5046 5192 387
Size: 1064 138 915 2168 374 146 1280
Fragments arranged by size:
2168 1280 1064 915 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 2096 3263 4148 4745 5532 5800
Size: 393 429 35 1167 885 597 787 268
Cuts at: 5800 1239
Size: 1524
Fragments arranged by size:
1524 1167 885 787 597 429 393 268
35
EcoO109I rG'GnC_Cy
Cuts at: 2482 2755 2807 3337 2482
Size: 273 52 530 5230
Fragments arranged by size:
5230 530 273 52
EcoRI G'AATT_C
Cuts at: 2068 3394 3772 2068
Size: 1326 378 4381
Fragments arranged by size:
4381 1326 378
FspI TGC'GCA
Cuts at: 4591 4591
Size: 6085
HaeII r_GCGC'y
Cuts at: 1678 5232 1678
Size: 3554 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4726 4758 4800 4832 1373
Size: 32 135 32 3154 32 42 32 2626
Fragments arranged by size:
3154 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3429 3675 669
Size: 988 281 1491 246 3079
Fragments arranged by size:
3079 1491 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6085
HpaI GTT'AAC
Cuts at: 3675 3675
Size: 6085
KpnI G_GTAC'C
Cuts at: 3410 3410
Size: 6085
MfeI C'AATT_G
Cuts at: 3684 3684
Size: 6085
MluI A'CGCG_T
Cuts at: 5961 5961
Size: 6085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5199 5383 1305
Size: 3894 184 2007
Fragments arranged by size:
3894 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2406 10
Size: 54 1425 343 574 3689
Fragments arranged by size:
3689 1425 574 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3230 4043 4402 4561 518
Size: 648 2064 813 359 159 2042
Fragments arranged by size:
2064 2042 813 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2149 2236 2312 2702 3501 4178 5326 5571
Size: 543 87 76 390 799 677 1148 245
Cuts at: 5571 1606
Size: 2120
Fragments arranged by size:
2120 1148 799 677 543 390 245 87
76
NaeI GCC'GGC
Cuts at: 2198 2317 2198
Size: 119 5966
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4594
Fragments arranged by size:
4594 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6085
NgoMIV G'CCGG_C
Cuts at: 2196 2315 2196
Size: 119 5966
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6085
NotI GC'GGCC_GC
Cuts at: 3532 3532
Size: 6085
NspI r_CATG'y
Cuts at: 1132 1229 2583 3443 4988 1132
Size: 97 1354 860 1545 2229
Fragments arranged by size:
2229 1545 1354 860 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6085
PciI A'CATG_T
Cuts at: 4984 4984
Size: 6085
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6085
PmeI GTTT'AAAC
Cuts at: 3389 3389
Size: 6085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4154 4186 5667 5699 4154
Size: 32 1481 32 4540
Fragments arranged by size:
4540 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2755 2755
Size: 6085
PshAI GACnn'nnGTC
Cuts at: 1613 3096 1613
Size: 1483 4602
PsiI TTA'TAA
Cuts at: 3655 3655
Size: 6085
PspOMI G'GGCC_C
Cuts at: 2806 2806
Size: 6085
PstI C_TGCA'G
Cuts at: 830 2772 3437 830
Size: 1942 665 3478
Fragments arranged by size:
3478 1942 665
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6085
PvuII CAG'CTG
Cuts at: 2236 2236
Size: 6085
SalI G'TCGA_C
Cuts at: 1936 3427 1936
Size: 1491 4594
SbfI CC_TGCA'GG
Cuts at: 2772 3437 2772
Size: 665 5420
ScaI AGT'ACT
Cuts at: 2303 4333 6045 2303
Size: 2030 1712 2343
Fragments arranged by size:
2343 2030 1712
SexAI A'CCwGG_T
Cuts at: 2508 2565 2508
Size: 57 6028
SfcI C'TryA_G
Cuts at: 826 1045 2191 2768 3433 4568 5249 5440 826
Size: 219 1146 577 665 1135 681 191 1471
Fragments arranged by size:
1471 1146 1135 681 665 577 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2331 2423 2331
Size: 92 5993
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6085
SmaI CCC'GGG
Cuts at: 1872 2015 3412 1872
Size: 143 1397 4545
Fragments arranged by size:
4545 1397 143
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3297 4189 5090 5352 820
Size: 197 926 1354 892 901 262 1553
Fragments arranged by size:
1553 1354 926 901 892 262 197
SnaBI TAC'GTA
Cuts at: 493 3876 493
Size: 3383 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6085
SphI G_CATG'C
Cuts at: 1132 3443 1132
Size: 2311 3774
SspI AAT'ATT
Cuts at: 5 52 4009 5
Size: 47 3957 2081
Fragments arranged by size:
3957 2081 47
StuI AGG'CCT
Cuts at: 1517 2417 2774 3076 1517
Size: 900 357 302 4526
Fragments arranged by size:
4526 900 357 302
StyI C'CwwG_G
Cuts at: 513 1065 2004 2329 2412 2775 3524 513
Size: 552 939 325 83 363 749 3074
Fragments arranged by size:
3074 939 749 552 363 325 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4112 4129 4282 4467 4912 325
Size: 1255 2532 17 153 185 445 1498
Fragments arranged by size:
2532 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2301 4331 6043
Size: 274 80 33 51 1443 324 2030 1712
Cuts at: 6043 96
Size: 138
Fragments arranged by size:
2030 1712 1443 324 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6045 6077 6045
Size: 32 6053
Tth111I GACn'n_nGTC
Cuts at: 1316 2510 1316
Size: 1194 4891
XbaI T'CTAG_A
Cuts at: 3421 3421
Size: 6085
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2384 2563 2657 2384
Size: 179 94 5812
Fragments arranged by size:
5812 179 94
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6085
XmaI C'CCGG_G
Cuts at: 1870 2013 3410 1870
Size: 143 1397 4545
Fragments arranged by size:
4545 1397 143
XmnI GAAnn'nnTTC
Cuts at: 4214 4214
Size: 6085
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3070 3893 276
Size: 53 83 186 936 1536 823 2468
Fragments arranged by size:
2468 1536 936 823 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SfoI SrfI SwaI