Restriction map for
pFN21AB9868
(Circular) (Six-base) MAPSORT of: pFN21AB9868.seq Check: 6357 from: 1 to: 6055
pFN21AB9868 6055 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2086 2086
Size: 6055
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3865 278
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2649 3398 1937
Size: 712 749 4594
Fragments arranged by size:
4594 749 712
Acc65I G'GTAC_C
Cuts at: 3376 3376
Size: 6055
AclI AA'CG_TT
Cuts at: 1563 4182 4555 1563
Size: 2619 373 3063
Fragments arranged by size:
3063 2619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2453 4118 5502 5770 1632
Size: 821 1665 1384 268 1917
Fragments arranged by size:
1917 1665 1384 821 268
AfeI AGC'GCT
Cuts at: 3116 3116
Size: 6055
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5858
AflIII A'CryG_T
Cuts at: 1203 4954 5931 1203
Size: 3751 977 1327
Fragments arranged by size:
3751 1327 977
AgeI A'CCGG_T
Cuts at: 4886 4886
Size: 6055
AhdI GACnn_n'nnGTC
Cuts at: 4784 4784
Size: 6055
AleI CACnn'nnGTG
Cuts at: 1166 2687 3125 1166
Size: 1521 438 4096
Fragments arranged by size:
4096 1521 438
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2705 1184
Size: 1521 4534
AlwNI CAG_nnn'CTG
Cuts at: 3798 5370 3798
Size: 1572 4483
ApaI G_GGCC'C
Cuts at: 2768 2786 2928 2768
Size: 18 142 5895
Fragments arranged by size:
5895 142 18
ApaLI G'TGCA_C
Cuts at: 2369 4112 5268 2369
Size: 1743 1156 3156
Fragments arranged by size:
3156 1743 1156
ApoI r'AATT_y
Cuts at: 1493 3230 3364 3590 3742 5851 1493
Size: 1737 134 226 152 2109 1697
Fragments arranged by size:
2109 1737 1697 226 152 134
AseI AT'TA_AT
Cuts at: 160 1032 4609 160
Size: 872 3577 1606
Fragments arranged by size:
3577 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
AvaI C'yCGr_G
Cuts at: 1870 1943 2073 2768 2785 2936 2949 2967 3380
Size: 73 130 695 17 151 13 18 413
Cuts at: 3380 1870
Size: 4545
Fragments arranged by size:
4545 695 413 151 130 73 18 17
13
BamHI G'GATC_C
Cuts at: 3385 3752 3385
Size: 367 5688
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 2069 2200 2738 2828
Size: 325 217 627 269 13 131 538 90
Cuts at: 2828 3376 4831 618
Size: 548 1455 1842
Fragments arranged by size:
1842 1455 627 548 538 325 269 217
131 90 13
BanII G_rGCy'C
Cuts at: 1391 2012 2768 2786 2844 2894 2928 3374 1391
Size: 621 756 18 58 50 34 446 4072
Fragments arranged by size:
4072 756 621 446 58 50 34 18
BbeI G_GCGC'C
Cuts at: 2060 2073 2204 2832 2060
Size: 13 131 628 5283
Fragments arranged by size:
5283 628 131 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2889 928
Size: 971 990 4094
Fragments arranged by size:
4094 990 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2143 2177 4246 4280 1903
Size: 34 206 34 2069 34 3678
Fragments arranged by size:
3678 2069 206 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3947 5157 3947
Size: 1210 4845
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6055
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2124 2790 2854
Size: 107 122 71 631 605 452 666 64
Cuts at: 2854 2923 4666 136
Size: 69 1743 1525
Fragments arranged by size:
1743 1525 666 631 605 452 122 107
71 69 64
BglII A'GATC_T
Cuts at: 2325 6050 2325
Size: 3725 2330
BlpI GC'TnA_GC
Cuts at: 3472 3472
Size: 6055
Bme1580I G_kGCm'C
Cuts at: 1790 2373 2741 2768 2786 2928 4116 5272 1790
Size: 583 368 27 18 142 1188 1156 2573
Fragments arranged by size:
2573 1188 1156 583 368 142 27 18
BmgBI CAC'GTC
Cuts at: 1318 2344 2358 1318
Size: 1026 14 5015
Fragments arranged by size:
5015 1026 14
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3786 4744 442
Size: 416 53 83 399 24 2369 958 1753
Fragments arranged by size:
2369 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6055
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2908 2908
Size: 6055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2903 2904 4715 1239
Size: 1664 1 1811 2579
Fragments arranged by size:
2579 1811 1664 1
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2180 4180 5045 5343 2180
Size: 2000 865 298 2892
Fragments arranged by size:
2892 2000 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4718 882
Size: 617 3219 2219
Fragments arranged by size:
3219 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2653 3846 493
Size: 711 1449 1193 2702
Fragments arranged by size:
2702 1449 1193 711
BsaBI GATnn'nnATC
Cuts at: 6049 6049
Size: 6055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2057 2070 2201 2715
Size: 53 83 186 936 524 13 131 514
Cuts at: 2715 2829 3035 3080 3862 4244 4879 275
Size: 114 206 45 782 382 635 1451
Fragments arranged by size:
1451 936 782 635 524 514 382 206
186 131 114 83 53 45 13
BsaWI w'CCGG_w
Cuts at: 2710 4488 4886 5160 5307 5718 2710
Size: 1778 398 274 147 411 3047
Fragments arranged by size:
3047 1778 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2879 2909 3138 3168 1178
Size: 30 165 30 1476 30 229 30 4065
Fragments arranged by size:
4065 1476 229 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2900 3071 1186
Size: 1714 171 4170
Fragments arranged by size:
4170 1714 171
BseYI C'CCAG_C
Cuts at: 2696 2817 5258 5754 2696
Size: 121 2441 496 2997
Fragments arranged by size:
2997 2441 496 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2338 1163
Size: 573 602 4880
Fragments arranged by size:
4880 602 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2238 2490 2556 2853 3198 3505
Size: 275 186 237 252 66 297 345 307
Cuts at: 3505 4266 5294 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 345 307 297 275 252
237 186 66
BsiHKAI G_wGCw'C
Cuts at: 2163 2349 2373 2894 3209 4116 4201 5272 2163
Size: 186 24 521 315 907 85 1071 2946
Fragments arranged by size:
2946 1071 907 521 315 186 85 24
BsmI GAATG_Cn'
Cuts at: 3566 3659 3566
Size: 93 5962
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2163 2349 2373 2741 2768 2786
Size: 399 222 151 186 24 368 27 18
Cuts at: 2786 2844 2894 2928 3209 3374 4116 4201 5272
Size: 58 50 34 281 165 742 85 1071
Cuts at: 5272 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 368 281 222 186
165 151 85 58 50 34 27 24
18
BspEI T'CCGG_A
Cuts at: 2710 5718 2710
Size: 3008 3047
BspHI T'CATG_A
Cuts at: 2637 3944 5674 2637
Size: 1307 1730 3018
Fragments arranged by size:
3018 1730 1307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2086 3410 844
Size: 1242 1324 3489
Fragments arranged by size:
3489 1324 1242
BsrBI CCG'CTC
Cuts at: 1394 1823 2509 2914 3942 1394
Size: 429 686 405 1028 3507
Fragments arranged by size:
3507 1028 686 429 405
BsrDI GCAATG_nn'
Cuts at: 65 1248 4550 4724 65
Size: 1183 3302 174 1396
Fragments arranged by size:
3302 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2123 2551 2848 3037 4699 4886 1700
Size: 110 313 428 297 189 1662 187 2869
Fragments arranged by size:
2869 1662 428 313 297 189 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6055
BssHII G'CGCG_C
Cuts at: 1925 2190 1925
Size: 265 5790
BssSI C'ACGA_G
Cuts at: 2182 4115 5127 2182
Size: 1933 1012 3110
Fragments arranged by size:
3110 1933 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6055
BstBI TT'CG_AA
Cuts at: 4859 4859
Size: 6055
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6055
BstXI CCAn_nnnn'nTGG
Cuts at: 2389 2761 2389
Size: 372 5683
BstYI r'GATC_y
Cuts at: 1972 2325 3321 3385 3752 4137 4154 5606 5692
Size: 353 996 64 367 385 17 1452 86
Cuts at: 5692 5704 6050 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 996 385 367 353 346 86
64 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3002 3135 513
Size: 552 939 998 133 3433
Fragments arranged by size:
3433 998 939 552 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2155 487
Size: 683 56 929 4387
Fragments arranged by size:
4387 929 683 56
BtsI GCAGTG_nn'
Cuts at: 3571 4365 4385 3571
Size: 794 20 5241
Fragments arranged by size:
5241 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6055
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6020
DraI TTT'AAA
Cuts at: 3359 3359
Size: 6055
DraIII CAC_nnn'GTG
Cuts at: 2475 2475
Size: 6055
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5062 809
Size: 507 3746 1802
Fragments arranged by size:
3746 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2235 2250
Size: 54 1425 50 275 18 291 114 15
Cuts at: 2250 2277 2487 2553 2850 3132 3195 3502 4391
Size: 27 210 66 297 282 63 307 889
Cuts at: 4391 6020 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 307 297 291 282 275
210 114 66 63 54 50 43 27
18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2235 2487 2553 2850 3195 3502 1537
Size: 275 423 252 66 297 345 307 4090
Fragments arranged by size:
4090 423 345 307 297 275 252 66
EarI CTCTTCn'nnn_
Cuts at: 1353 2219 2354 3230 3985 1353
Size: 866 135 876 755 3423
Fragments arranged by size:
3423 876 866 755 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3293 4642 5016 5162 387
Size: 1064 138 1704 1349 374 146 1280
Fragments arranged by size:
1704 1349 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2892 2892
Size: 6055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2453 2903 2904 4118 4715 5502 5770
Size: 393 821 450 1 1214 597 787 268
Cuts at: 5770 1239
Size: 1524
Fragments arranged by size:
1524 1214 821 787 597 450 393 268
1
EcoO109I rG'GnC_Cy
Cuts at: 2765 2924 2925 2765
Size: 159 1 5895
Fragments arranged by size:
5895 159 1
EcoRI G'AATT_C
Cuts at: 3364 3742 3364
Size: 378 5677
FseI GG_CCGG'CC
Cuts at: 2127 2127
Size: 6055
FspI TGC'GCA
Cuts at: 2657 4561 2657
Size: 1904 4151
FspAI rTGC'GCAy
Cuts at: 2657 2657
Size: 6055
HaeII r_GCGC'y
Cuts at: 1678 2060 2073 2204 2339 2832 3118 5202 1678
Size: 382 13 131 135 493 286 2084 2531
Fragments arranged by size:
2531 2084 493 382 286 135 131 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2879 2911 3313 3345 4696
Size: 32 135 32 1307 32 402 32 1351
Cuts at: 4696 4728 4770 4802 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1351 1307 402 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3399 3645 669
Size: 988 281 1461 246 3079
Fragments arranged by size:
3079 1461 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6055
HpaI GTT'AAC
Cuts at: 3645 3645
Size: 6055
KasI G'GCGC_C
Cuts at: 2056 2069 2200 2828 2056
Size: 13 131 628 5283
Fragments arranged by size:
5283 628 131 13
KpnI G_GTAC'C
Cuts at: 3380 3380
Size: 6055
MfeI C'AATT_G
Cuts at: 3654 3654
Size: 6055
MluI A'CGCG_T
Cuts at: 5931 5931
Size: 6055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2925 5169 5353 1305
Size: 1620 2244 184 2007
Fragments arranged by size:
2244 2007 1620 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2252 2279 10
Size: 54 1425 343 420 27 3786
Fragments arranged by size:
3786 1425 420 343 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 2246 2471 2687 3125 3146 4013
Size: 648 1008 72 225 216 438 21 867
Cuts at: 4013 4372 4531 518
Size: 359 159 2042
Fragments arranged by size:
2042 1008 867 648 438 359 225 216
159 72 21
MspA1I CmG'CkG
Cuts at: 1606 2809 3004 3471 4148 5296 5541 1606
Size: 1203 195 467 677 1148 245 2120
Fragments arranged by size:
2120 1203 1148 677 467 245 195
NaeI GCC'GGC
Cuts at: 2125 2553 2850 3039 2125
Size: 428 297 189 5141
Fragments arranged by size:
5141 428 297 189
NarI GG'CG_CC
Cuts at: 2057 2070 2201 2829 2057
Size: 13 131 628 5283
Fragments arranged by size:
5283 628 131 13
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4564
Fragments arranged by size:
4564 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6055
NgoMIV G'CCGG_C
Cuts at: 2123 2551 2848 3037 2123
Size: 428 297 189 5141
Fragments arranged by size:
5141 428 297 189
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6055
NotI GC'GGCC_GC
Cuts at: 3502 3502
Size: 6055
NspI r_CATG'y
Cuts at: 1132 1229 3413 4958 1132
Size: 97 2184 1545 2229
Fragments arranged by size:
2229 2184 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6055
PciI A'CATG_T
Cuts at: 4954 4954
Size: 6055
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6055
PmeI GTTT'AAAC
Cuts at: 3359 3359
Size: 6055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4124 4156 5637 5669 4124
Size: 32 1481 32 4510
Fragments arranged by size:
4510 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6055
PsiI TTA'TAA
Cuts at: 3625 3625
Size: 6055
PspOMI G'GGCC_C
Cuts at: 2764 2782 2924 2764
Size: 18 142 5895
Fragments arranged by size:
5895 142 18
PstI C_TGCA'G
Cuts at: 830 2019 2097 3046 3407 830
Size: 1189 78 949 361 3478
Fragments arranged by size:
3478 1189 949 361 78
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6055
SacI G_AGCT'C
Cuts at: 2894 2894
Size: 6055
SacII CC_GC'GG
Cuts at: 3005 3005
Size: 6055
SalI G'TCGA_C
Cuts at: 1936 3397 1936
Size: 1461 4594
SapI GCTCTTCn'nnn_
Cuts at: 2354 3230 2354
Size: 876 5179
SbfI CC_TGCA'GG
Cuts at: 2097 3407 2097
Size: 1310 4745
ScaI AGT'ACT
Cuts at: 4303 6015 4303
Size: 1712 4343
SfcI C'TryA_G
Cuts at: 826 1045 2015 2093 3042 3403 4538 5219 5410
Size: 219 970 78 949 361 1135 681 191
Cuts at: 5410 826
Size: 1471
Fragments arranged by size:
1471 1135 970 949 681 361 219 191
78
SfiI GGCCn_nnn'nGGCC
Cuts at: 2923 2923
Size: 6055
SfoI GGC'GCC
Cuts at: 2058 2071 2202 2830 2058
Size: 13 131 628 5283
Fragments arranged by size:
5283 628 131 13
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6055
SmaI CCC'GGG
Cuts at: 1872 2075 2787 2951 3382 1872
Size: 203 712 164 431 4545
Fragments arranged by size:
4545 712 431 203 164
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2195 4159 5060 5322 820
Size: 197 926 252 1964 901 262 1553
Fragments arranged by size:
1964 1553 926 901 262 252 197
SnaBI TAC'GTA
Cuts at: 493 3846 493
Size: 3353 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6055
SphI G_CATG'C
Cuts at: 1132 3413 1132
Size: 2281 3774
SrfI GCCC'GGGC
Cuts at: 2075 2075
Size: 6055
SspI AAT'ATT
Cuts at: 5 52 3979 5
Size: 47 3927 2081
Fragments arranged by size:
3927 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6055
StyI C'CwwG_G
Cuts at: 513 1065 2004 3075 3494 513
Size: 552 939 1071 419 3074
Fragments arranged by size:
3074 1071 939 552 419
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2066 2710 2831 4082 4099 4252 4437
Size: 1255 486 644 121 1251 17 153 185
Cuts at: 4437 4882 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1251 644 486 445 185 153
121 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2424 4301 6013
Size: 274 80 33 51 1443 447 1877 1712
Cuts at: 6013 96
Size: 138
Fragments arranged by size:
1877 1712 1443 447 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3138 3170 6015 6047 3138
Size: 32 2845 32 3146
Fragments arranged by size:
3146 2845 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2717 1316
Size: 1401 4654
XbaI T'CTAG_A
Cuts at: 3391 3391
Size: 6055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2287 2287
Size: 6055
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6055
XmaI C'CCGG_G
Cuts at: 1870 2073 2785 2949 3380 1870
Size: 203 712 164 431 4545
Fragments arranged by size:
4545 712 431 203 164
XmnI GAAnn'nnTTC
Cuts at: 3116 4184 3116
Size: 1068 4987
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3863 276
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SanDI SexAI SwaI