Restriction map for
pFN21AB9865
(Circular) (Six-base) MAPSORT of: pFN21AB9865.seq Check: 4361 from: 1 to: 6001
pFN21AB9865 6001 bp
With 182 enzymes: SgfI *
February 27, 2009 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2115 2371 2115
Size: 256 5745
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3811 278
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3344 1937
Size: 1407 4594
Acc65I G'GTAC_C
Cuts at: 3322 3322
Size: 6001
AclI AA'CG_TT
Cuts at: 1563 4128 4501 1563
Size: 2565 373 3063
Fragments arranged by size:
3063 2565 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4064 5448 5716 1632
Size: 2432 1384 268 1917
Fragments arranged by size:
2432 1917 1384 268
AfeI AGC'GCT
Cuts at: 2201 2201
Size: 6001
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5804
AflIII A'CryG_T
Cuts at: 1203 2214 4900 5877 1203
Size: 1011 2686 977 1327
Fragments arranged by size:
2686 1327 1011 977
AgeI A'CCGG_T
Cuts at: 4832 4832
Size: 6001
AhdI GACnn_n'nnGTC
Cuts at: 2191 4730 2191
Size: 2539 3462
AleI CACnn'nnGTG
Cuts at: 1166 2228 1166
Size: 1062 4939
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6001
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3083 3115 3083
Size: 32 5969
AlwNI CAG_nnn'CTG
Cuts at: 2584 2866 3744 5316 2584
Size: 282 878 1572 3269
Fragments arranged by size:
3269 1572 878 282
ApaLI G'TGCA_C
Cuts at: 2111 2384 4058 5214 2111
Size: 273 1674 1156 2898
Fragments arranged by size:
2898 1674 1156 273
ApoI r'AATT_y
Cuts at: 1493 3310 3536 3688 5797 1493
Size: 1817 226 152 2109 1697
Fragments arranged by size:
2109 1817 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4555 160
Size: 872 3523 1606
Fragments arranged by size:
3523 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
AvaI C'yCGr_G
Cuts at: 1870 1943 3326 1870
Size: 73 1383 4545
Fragments arranged by size:
4545 1383 73
BamHI G'GATC_C
Cuts at: 3331 3698 3331
Size: 367 5634
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 2127 2537 3052 3322
Size: 325 217 627 297 43 410 515 270
Cuts at: 3322 4777 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 515 410 325 297 270
217 43
BanII G_rGCy'C
Cuts at: 1391 2991 3320 1391
Size: 1600 329 4072
Fragments arranged by size:
4072 1600 329
BbeI G_GCGC'C
Cuts at: 2088 2088
Size: 6001
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2473 928
Size: 971 574 4456
Fragments arranged by size:
4456 971 574
BbvCI CC'TCA_GC
Cuts at: 2349 2349
Size: 6001
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2122 2156 4192 4226 1903
Size: 34 185 34 2036 34 3678
Fragments arranged by size:
3678 2036 185 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3893 5103 3893
Size: 1210 4791
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6001
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2021 2546 2555
Size: 107 122 71 631 605 349 525 9
Cuts at: 2555 2609 3207 4612 136
Size: 54 598 1405 1525
Fragments arranged by size:
1525 1405 631 605 598 525 349 122
107 71 54 9
BglII A'GATC_T
Cuts at: 5996 5996
Size: 6001
BlpI GC'TnA_GC
Cuts at: 3418 3418
Size: 6001
Bme1580I G_kGCm'C
Cuts at: 1790 2115 2388 2471 2578 3057 4062 5218 1790
Size: 325 273 83 107 479 1005 1156 2573
Fragments arranged by size:
2573 1156 1005 479 325 273 107 83
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6001
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2491 3732 4690
Size: 416 53 83 399 24 1074 1241 958
Cuts at: 4690 442
Size: 1753
Fragments arranged by size:
1753 1241 1074 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6001
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3073 3073
Size: 6001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2360 2888 3230 3287 4661 1239
Size: 1121 528 342 57 1374 2579
Fragments arranged by size:
2579 1374 1121 528 342 57
Bpu10I CC'TnA_GC
Cuts at: 2349 3152 2349
Size: 803 5198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4126 4991 5289 4126
Size: 865 298 4838
Fragments arranged by size:
4838 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3177 4664 882
Size: 617 1678 1487 2219
Fragments arranged by size:
2219 1678 1487 617
BsaAI yAC'GTr
Cuts at: 493 1204 3792 493
Size: 711 2588 2702
Fragments arranged by size:
2702 2588 711
BsaBI GATnn'nnATC
Cuts at: 2753 5995 2753
Size: 3242 2759
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 3808 4190 4825
Size: 53 83 186 936 552 1723 382 635
Cuts at: 4825 275
Size: 1451
Fragments arranged by size:
1723 1451 936 635 552 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4434 4832 5106 5253 5664 4434
Size: 398 274 147 411 4771
Fragments arranged by size:
4771 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2714 2744 3083 3113 1178
Size: 30 165 30 1311 30 339 30 4066
Fragments arranged by size:
4066 1311 339 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2477 2735 3179 1186
Size: 880 411 258 444 4008
Fragments arranged by size:
4008 880 444 411 258
BseYI C'CCAG_C
Cuts at: 5204 5700 5204
Size: 496 5505
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2048 1163
Size: 573 312 5116
Fragments arranged by size:
5116 573 312
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3451 4212 5240 1540
Size: 275 186 1450 761 1028 2301
Fragments arranged by size:
2301 1450 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2115 2388 2997 4062 4147 5218 2115
Size: 273 609 1065 85 1071 2898
Fragments arranged by size:
2898 1071 1065 609 273 85
BsmI GAATG_Cn'
Cuts at: 3512 3605 3512
Size: 93 5908
BsmBI CGTCTCn'nnnn_
Cuts at: 2414 2864 2414
Size: 450 5551
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2388 2471 2578 2991 2997 3057
Size: 399 325 273 83 107 413 6 60
Cuts at: 3057 3320 4062 4147 5218 1391
Size: 263 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 413 399 325 273 263
107 85 83 60 6
BspEI T'CCGG_A
Cuts at: 5664 5664
Size: 6001
BspHI T'CATG_A
Cuts at: 2529 3890 5620 2529
Size: 1361 1730 2910
Fragments arranged by size:
2910 1730 1361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2115 2371 2664 3356 844
Size: 1271 256 293 692 3489
Fragments arranged by size:
3489 1271 692 293 256
BsrBI CCG'CTC
Cuts at: 1394 1823 2842 3888 1394
Size: 429 1019 1046 3507
Fragments arranged by size:
3507 1046 1019 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4496 4670 65
Size: 1183 3248 174 1396
Fragments arranged by size:
3248 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2344 2856 4645 4832 1700
Size: 110 534 512 1789 187 2869
Fragments arranged by size:
2869 1789 534 512 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6001
BssHII G'CGCG_C
Cuts at: 1925 2689 2691 1925
Size: 764 2 5235
Fragments arranged by size:
5235 764 2
BssSI C'ACGA_G
Cuts at: 4061 5073 4061
Size: 1012 4989
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2631 794
Size: 1837 4164
BstBI TT'CG_AA
Cuts at: 4805 4805
Size: 6001
BstEII G'GTnAC_C
Cuts at: 1184 2071 2333 2651 2713 2975 1184
Size: 887 262 318 62 262 4210
Fragments arranged by size:
4210 887 318 262 262 62
BstXI CCAn_nnnn'nTGG
Cuts at: 2230 3254 2230
Size: 1024 4977
BstYI r'GATC_y
Cuts at: 1972 2964 3082 3331 3698 4083 4100 5552 5638
Size: 992 118 249 367 385 17 1452 86
Cuts at: 5638 5650 5996 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 992 385 367 346 249 118
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2010 2604 2708 513
Size: 552 945 594 104 3806
Fragments arranged by size:
3806 945 594 552 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2302 487
Size: 683 56 1076 4186
Fragments arranged by size:
4186 1076 683 56
BtsI GCAGTG_nn'
Cuts at: 3517 4311 4331 3517
Size: 794 20 5187
Fragments arranged by size:
5187 794 20
ClaI AT'CG_AT
Cuts at: 1554 2757 1554
Size: 1203 4798
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5966
DraI TTT'AAA
Cuts at: 3305 3305
Size: 6001
DraIII CAC_nnn'GTG
Cuts at: 2333 2372 2674 2333
Size: 39 302 5660
Fragments arranged by size:
5660 302 39
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5008 809
Size: 507 3692 1802
Fragments arranged by size:
3692 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2155 2475
Size: 54 1425 50 275 18 183 142 320
Cuts at: 2475 2547 2955 3193 3448 4337 5966 8
Size: 72 408 238 255 889 1629 43
Fragments arranged by size:
1629 1425 889 408 320 275 255 238
183 142 72 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3448 1537
Size: 275 1636 4090
Fragments arranged by size:
4090 1636 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2414 2849 3002 3931 1353
Size: 1061 435 153 929 3423
Fragments arranged by size:
3423 1061 929 435 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3191 4588 4962 5108 387
Size: 1064 138 1602 1397 374 146 1280
Fragments arranged by size:
1602 1397 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2360 2888 3230 3287 4064 4661 5448
Size: 393 728 528 342 57 777 597 787
Cuts at: 5448 5716 1239
Size: 268 1524
Fragments arranged by size:
1524 787 777 728 597 528 393 342
268 57
EcoO109I rG'GnC_Cy
Cuts at: 2776 2863 3262 2776
Size: 87 399 5515
Fragments arranged by size:
5515 399 87
EcoRI G'AATT_C
Cuts at: 3310 3688 3310
Size: 378 5623
FspI TGC'GCA
Cuts at: 2660 4507 2660
Size: 1847 4154
HaeII r_GCGC'y
Cuts at: 1678 2088 2203 5148 1678
Size: 410 115 2945 2531
Fragments arranged by size:
2945 2531 410 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2177 2209 4642 4674 4716
Size: 32 135 32 605 32 2433 32 42
Cuts at: 4716 4748 1373
Size: 32 2626
Fragments arranged by size:
2626 2433 605 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3345 3591 669
Size: 988 281 1407 246 3079
Fragments arranged by size:
3079 1407 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6001
HpaI GTT'AAC
Cuts at: 3591 3591
Size: 6001
KasI G'GCGC_C
Cuts at: 2084 2084
Size: 6001
KpnI G_GTAC'C
Cuts at: 3326 3326
Size: 6001
MfeI C'AATT_G
Cuts at: 3600 3600
Size: 6001
MluI A'CGCG_T
Cuts at: 5877 5877
Size: 6001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5115 5299 1305
Size: 3810 184 2007
Fragments arranged by size:
3810 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2157 2549 2957 10
Size: 54 1425 343 325 392 408 3054
Fragments arranged by size:
3054 1425 408 392 343 325 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2228 2321 2489 2759 2924 3959 4318
Size: 648 1062 93 168 270 165 1035 359
Cuts at: 4318 4477 518
Size: 159 2042
Fragments arranged by size:
2042 1062 1035 648 359 270 168 165
159 93
MspA1I CmG'CkG
Cuts at: 1606 2012 2241 2710 2740 3069 3274 3417 4094
Size: 406 229 469 30 329 205 143 677
Cuts at: 4094 5242 5487 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 469 406 329 245 229
205 143 30
NaeI GCC'GGC
Cuts at: 2346 2858 2346
Size: 512 5489
NarI GG'CG_CC
Cuts at: 2085 2085
Size: 6001
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5449
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6001
NgoMIV G'CCGG_C
Cuts at: 2344 2856 2344
Size: 512 5489
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6001
NotI GC'GGCC_GC
Cuts at: 3448 3448
Size: 6001
NspI r_CATG'y
Cuts at: 1132 1229 2218 2254 3359 4904 1132
Size: 97 989 36 1105 1545 2229
Fragments arranged by size:
2229 1545 1105 989 97 36
PasI CC'CwG_GG
Cuts at: 1348 3111 1348
Size: 1763 4238
PciI A'CATG_T
Cuts at: 2214 4900 2214
Size: 2686 3315
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2674 1108
Size: 1566 4435
PfoI T'CCnGG_A
Cuts at: 2898 3264 2898
Size: 366 5635
PmeI GTTT'AAAC
Cuts at: 3305 3305
Size: 6001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3083 3115 4070 4102 5583 5615 3083
Size: 32 955 32 1481 32 3469
Fragments arranged by size:
3469 1481 955 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2863 3262 2863
Size: 399 5602
PshAI GACnn'nnGTC
Cuts at: 1613 3289 1613
Size: 1676 4325
PsiI TTA'TAA
Cuts at: 3571 3571
Size: 6001
PstI C_TGCA'G
Cuts at: 830 3074 3353 830
Size: 2244 279 3478
Fragments arranged by size:
3478 2244 279
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6001
SacII CC_GC'GG
Cuts at: 2013 2711 2013
Size: 698 5303
SalI G'TCGA_C
Cuts at: 1936 3343 1936
Size: 1407 4594
SapI GCTCTTCn'nnn_
Cuts at: 2849 3002 2849
Size: 153 5848
SbfI CC_TGCA'GG
Cuts at: 3353 3353
Size: 6001
ScaI AGT'ACT
Cuts at: 4249 5961 4249
Size: 1712 4289
SexAI A'CCwGG_T
Cuts at: 2067 2067
Size: 6001
SfcI C'TryA_G
Cuts at: 826 1045 2032 3070 3280 3349 4484 5165 5356
Size: 219 987 1038 210 69 1135 681 191
Cuts at: 5356 826
Size: 1471
Fragments arranged by size:
1471 1135 1038 987 681 219 210 191
69
SfiI GGCCn_nnn'nGGCC
Cuts at: 2021 2021
Size: 6001
SfoI GGC'GCC
Cuts at: 2086 2086
Size: 6001
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6001
SmaI CCC'GGG
Cuts at: 1872 3328 1872
Size: 1456 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4105 5006 5268 820
Size: 197 926 2162 901 262 1553
Fragments arranged by size:
2162 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3792 493
Size: 3299 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6001
SphI G_CATG'C
Cuts at: 1132 3359 1132
Size: 2227 3774
SspI AAT'ATT
Cuts at: 5 52 3925 5
Size: 47 3873 2081
Fragments arranged by size:
3873 2081 47
StuI AGG'CCT
Cuts at: 1517 3210 1517
Size: 1693 4308
StyI C'CwwG_G
Cuts at: 513 1065 3440 513
Size: 552 2375 3074
Fragments arranged by size:
3074 2375 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2948 4028 4045 4198 4383 4828 325
Size: 1255 1368 1080 17 153 185 445 1498
Fragments arranged by size:
1498 1368 1255 1080 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2730 3012 4247
Size: 274 80 33 51 1443 753 282 1235
Cuts at: 4247 5959 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1235 753 282 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5961 5993 5961
Size: 32 5969
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6001
XbaI T'CTAG_A
Cuts at: 3337 3337
Size: 6001
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6001
XmaI C'CCGG_G
Cuts at: 1870 3326 1870
Size: 1456 4545
XmnI GAAnn'nnTTC
Cuts at: 3098 4130 3098
Size: 1032 4969
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3809 276
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SanDI SrfI SwaI XcmI