Restriction map for
pFN21AB9864
(Circular) (Six-base) MAPSORT of: pFN21AB9864.seq Check: 2148 from: 1 to: 5971
pFN21AB9864 5971 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 278
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3314 1937
Size: 1377 4594
Acc65I G'GTAC_C
Cuts at: 3292 3292
Size: 5971
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4034 5418 5686 1632
Size: 2402 1384 268 1917
Fragments arranged by size:
2402 1917 1384 268
AfeI AGC'GCT
Cuts at: 2198 2198
Size: 5971
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5774
AflIII A'CryG_T
Cuts at: 1203 3231 4870 5847 1203
Size: 2028 1639 977 1327
Fragments arranged by size:
2028 1639 1327 977
AgeI A'CCGG_T
Cuts at: 4802 4802
Size: 5971
AhdI GACnn_n'nnGTC
Cuts at: 4700 4700
Size: 5971
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5971
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5971
AlwNI CAG_nnn'CTG
Cuts at: 2409 2790 3714 5286 2409
Size: 381 924 1572 3094
Fragments arranged by size:
3094 1572 924 381
ApaLI G'TGCA_C
Cuts at: 2888 4028 5184 2888
Size: 1140 1156 3675
Fragments arranged by size:
3675 1156 1140
ApoI r'AATT_y
Cuts at: 1493 3280 3506 3658 5767 1493
Size: 1787 226 152 2109 1697
Fragments arranged by size:
2109 1787 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2458 2458
Size: 5971
AseI AT'TA_AT
Cuts at: 160 1032 4525 160
Size: 872 3493 1606
Fragments arranged by size:
3493 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 3018 3296 1870
Size: 73 1075 278 4545
Fragments arranged by size:
4545 1075 278 73
BamHI G'GATC_C
Cuts at: 3301 3668 3301
Size: 367 5604
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2864 3059 3149 3292 4747
Size: 325 217 627 1077 195 90 143 1455
Cuts at: 4747 618
Size: 1842
Fragments arranged by size:
1842 1455 1077 627 325 217 195 143
90
BanII G_rGCy'C
Cuts at: 1391 3290 1391
Size: 1899 4072
BbeI G_GCGC'C
Cuts at: 2868 3153 2868
Size: 285 5686
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2547 2714 3035 3044 928
Size: 971 648 167 321 9 3855
Fragments arranged by size:
3855 971 648 321 167 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4162 4196 1903
Size: 34 2225 34 3678
Fragments arranged by size:
3678 2225 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3863 5073 3863
Size: 1210 4761
BclI T'GATC_A
Cuts at: 1548 2115 1548
Size: 567 5404
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4582 136
Size: 107 122 71 631 605 2910 1525
Fragments arranged by size:
2910 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5966 5966
Size: 5971
BlpI GC'TnA_GC
Cuts at: 2015 3388 2015
Size: 1373 4598
Bme1580I G_kGCm'C
Cuts at: 1790 2138 2892 2956 3062 4032 5188 1790
Size: 348 754 64 106 970 1156 2573
Fragments arranged by size:
2573 1156 970 754 348 106 64
BmgBI CAC'GTC
Cuts at: 1318 2095 2353 1318
Size: 777 258 4936
Fragments arranged by size:
4936 777 258
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2324 2419 3702
Size: 416 53 83 399 24 907 95 1283
Cuts at: 3702 4660 442
Size: 958 1753
Fragments arranged by size:
1753 1283 958 907 416 399 95 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2812 2894 4631 1239
Size: 1573 82 1737 2579
Fragments arranged by size:
2579 1737 1573 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4096 4961 5259 4096
Size: 865 298 4808
Fragments arranged by size:
4808 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2057 4634 882
Size: 617 558 2577 2219
Fragments arranged by size:
2577 2219 617 558
BsaAI yAC'GTr
Cuts at: 493 1204 2437 3762 493
Size: 711 1233 1325 2702
Fragments arranged by size:
2702 1325 1233 711
BsaBI GATnn'nnATC
Cuts at: 5965 5965
Size: 5971
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2450 2865 3150 3778
Size: 53 83 186 936 917 415 285 628
Cuts at: 3778 4160 4795 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 917 635 628 415 382 285
186 83 53
BsaWI w'CCGG_w
Cuts at: 2711 4404 4802 5076 5223 5634 2711
Size: 1693 398 274 147 411 3048
Fragments arranged by size:
3048 1693 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5746
Fragments arranged by size:
5746 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5971
BseYI C'CCAG_C
Cuts at: 2131 2158 2521 2797 2816 2927 3043 3166 5174
Size: 27 363 276 19 111 116 123 2008
Cuts at: 5174 5670 2131
Size: 496 2432
Fragments arranged by size:
2432 2008 496 363 276 123 116 111
27 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2347 1163
Size: 573 611 4787
Fragments arranged by size:
4787 611 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3421 4182 5210 1540
Size: 275 186 1420 761 1028 2301
Fragments arranged by size:
2301 1420 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2016 2301 2827 2892 2916 4032 4117 5188 2016
Size: 285 526 65 24 1116 85 1071 2799
Fragments arranged by size:
2799 1116 1071 526 285 85 65 24
BsmI GAATG_Cn'
Cuts at: 3482 3575 3482
Size: 93 5878
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2138 2301 2827 2892 2916 2956
Size: 399 226 122 163 526 65 24 40
Cuts at: 2956 3062 3290 4032 4117 5188 1391
Size: 106 228 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 526 399 228 226 163
122 106 85 65 40 24
BspEI T'CCGG_A
Cuts at: 5634 5634
Size: 5971
BspHI T'CATG_A
Cuts at: 3860 5590 3860
Size: 1730 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2336 3326 844
Size: 1492 990 3489
Fragments arranged by size:
3489 1492 990
BsrBI CCG'CTC
Cuts at: 1394 1823 2547 2717 3858 1394
Size: 429 724 170 1141 3507
Fragments arranged by size:
3507 1141 724 429 170
BsrDI GCAATG_nn'
Cuts at: 65 1248 2170 4466 4640 65
Size: 1183 922 2296 174 1396
Fragments arranged by size:
2296 1396 1183 922 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2050 2066 4615 4802 1700
Size: 110 240 16 2549 187 2869
Fragments arranged by size:
2869 2549 240 187 110 16
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5971
BssHII G'CGCG_C
Cuts at: 1925 2458 2669 1925
Size: 533 211 5227
Fragments arranged by size:
5227 533 211
BssSI C'ACGA_G
Cuts at: 4031 5043 4031
Size: 1012 4959
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2169 794
Size: 1375 4596
BstBI TT'CG_AA
Cuts at: 4775 4775
Size: 5971
BstEII G'GTnAC_C
Cuts at: 1184 2242 2323 1184
Size: 1058 81 4832
Fragments arranged by size:
4832 1058 81
BstXI CCAn_nnnn'nTGG
Cuts at: 2191 2398 3145 2191
Size: 207 747 5017
Fragments arranged by size:
5017 747 207
BstYI r'GATC_y
Cuts at: 1972 3301 3668 4053 4070 5522 5608 5620 5966
Size: 1329 367 385 17 1452 86 12 346
Cuts at: 5966 1972
Size: 1977
Fragments arranged by size:
1977 1452 1329 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2277 2728 513
Size: 552 939 273 451 3756
Fragments arranged by size:
3756 939 552 451 273
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2170 2326 2377 487
Size: 683 56 944 156 51 4081
Fragments arranged by size:
4081 944 683 156 56 51
BtsI GCAGTG_nn'
Cuts at: 3487 4281 4301 3487
Size: 794 20 5157
Fragments arranged by size:
5157 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5936
DraI TTT'AAA
Cuts at: 3275 3275
Size: 5971
DraIII CAC_nnn'GTG
Cuts at: 2896 2896
Size: 5971
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2099 4978 809
Size: 507 783 2879 1802
Fragments arranged by size:
2879 1802 783 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2214 2280 3418
Size: 54 1425 50 275 18 384 66 1138
Cuts at: 3418 4307 5936 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1138 889 384 275 66 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3418 1537
Size: 275 1606 4090
Fragments arranged by size:
4090 1606 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2753 3901 1353
Size: 1400 1148 3423
Fragments arranged by size:
3423 1400 1148
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4558 4932 5078 387
Size: 1064 138 2969 374 146 1280
Fragments arranged by size:
2969 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2812 2894 4034 4631 5418 5686 1239
Size: 393 1180 82 1140 597 787 268 1524
Fragments arranged by size:
1524 1180 1140 787 597 393 268 82
EcoRI G'AATT_C
Cuts at: 3280 3658 3280
Size: 378 5593
FspI TGC'GCA
Cuts at: 4477 4477
Size: 5971
HaeII r_GCGC'y
Cuts at: 1678 2200 2868 3153 5118 1678
Size: 522 668 285 1965 2531
Fragments arranged by size:
2531 1965 668 522 285
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2107 2139 4612 4644 4686
Size: 32 135 32 535 32 2473 32 42
Cuts at: 4686 4718 1373
Size: 32 2626
Fragments arranged by size:
2626 2473 535 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3315 3561 669
Size: 988 281 1377 246 3079
Fragments arranged by size:
3079 1377 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5971
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KasI G'GCGC_C
Cuts at: 2864 3149 2864
Size: 285 5686
KpnI G_GTAC'C
Cuts at: 3296 3296
Size: 5971
MfeI C'AATT_G
Cuts at: 3160 3570 3160
Size: 410 5561
MluI A'CGCG_T
Cuts at: 3231 5847 3231
Size: 2616 3355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2115 3201 5085 5269 1305
Size: 810 1086 1884 184 2007
Fragments arranged by size:
2007 1884 1086 810 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2216 10
Size: 54 1425 343 384 3765
Fragments arranged by size:
3765 1425 384 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3053 3929 4288 4447 518
Size: 648 843 1044 876 359 159 2042
Fragments arranged by size:
2042 1044 876 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2465 2626 3126 3387 4064 5212 5457 1606
Size: 859 161 500 261 677 1148 245 2120
Fragments arranged by size:
2120 1148 859 677 500 261 245 161
NarI GG'CG_CC
Cuts at: 2865 3150 2865
Size: 285 5686
NcoI C'CATG_G
Cuts at: 513 1065 2004 2277 2728 513
Size: 552 939 273 451 3756
Fragments arranged by size:
3756 939 552 451 273
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5971
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5971
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 2565 3136 3329 4874 1132
Size: 97 1336 571 193 1545 2229
Fragments arranged by size:
2229 1545 1336 571 193 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5971
PciI A'CATG_T
Cuts at: 4870 4870
Size: 5971
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5971
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 5553 5585 4040
Size: 32 1481 32 4426
Fragments arranged by size:
4426 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5971
PsiI TTA'TAA
Cuts at: 3541 3541
Size: 5971
PstI C_TGCA'G
Cuts at: 830 2208 3323 830
Size: 1378 1115 3478
Fragments arranged by size:
3478 1378 1115
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
PvuII CAG'CTG
Cuts at: 3126 3126
Size: 5971
SalI G'TCGA_C
Cuts at: 1936 3313 1936
Size: 1377 4594
SbfI CC_TGCA'GG
Cuts at: 3323 3323
Size: 5971
ScaI AGT'ACT
Cuts at: 4219 5931 4219
Size: 1712 4259
SfcI C'TryA_G
Cuts at: 826 1045 2204 3319 4454 5135 5326 826
Size: 219 1159 1115 1135 681 191 1471
Fragments arranged by size:
1471 1159 1135 1115 681 219 191
SfoI GGC'GCC
Cuts at: 2866 3151 2866
Size: 285 5686
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 3298 1872
Size: 1426 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4075 4976 5238 820
Size: 197 926 2132 901 262 1553
Fragments arranged by size:
2132 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3762 493
Size: 3269 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 2565 3329 1132
Size: 1433 764 3774
Fragments arranged by size:
3774 1433 764
SspI AAT'ATT
Cuts at: 5 52 3895 5
Size: 47 3843 2081
Fragments arranged by size:
3843 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5971
StyI C'CwwG_G
Cuts at: 513 1065 2004 2190 2277 2728 3410 513
Size: 552 939 186 87 451 682 3074
Fragments arranged by size:
3074 939 682 552 451 186 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3998 4015 4168 4353 4798 325
Size: 1255 2418 17 153 185 445 1498
Fragments arranged by size:
2418 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4217 5929 96
Size: 274 80 33 51 1443 2240 1712 138
Fragments arranged by size:
2240 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5931 5963 5931
Size: 32 5939
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5971
XbaI T'CTAG_A
Cuts at: 3307 3307
Size: 5971
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5971
XmaI C'CCGG_G
Cuts at: 1870 3296 1870
Size: 1426 4545
XmnI GAAnn'nnTTC
Cuts at: 4100 4100
Size: 5971
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 276
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI