Restriction map for
pFN21AB9859
(Circular) (Six-base) MAPSORT of: pFN21AB9859.seq Check: 9858 from: 1 to: 5905
pFN21AB9859 5905 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3715 278
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3248 1937
Size: 1311 4594
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2140 2477 3968 5352 5620 1632
Size: 508 337 1491 1384 268 1917
Fragments arranged by size:
1917 1491 1384 508 337 268
AfeI AGC'GCT
Cuts at: 2165 2454 2165
Size: 289 5616
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 2748 4804 5781 1203
Size: 1545 2056 977 1327
Fragments arranged by size:
2056 1545 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 4634 4634
Size: 5905
AleI CACnn'nnGTG
Cuts at: 1166 2762 1166
Size: 1596 4309
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5905
AlwNI CAG_nnn'CTG
Cuts at: 2905 3648 5220 2905
Size: 743 1572 3590
Fragments arranged by size:
3590 1572 743
ApaI G_GGCC'C
Cuts at: 2143 2143
Size: 5905
ApaLI G'TGCA_C
Cuts at: 3962 5118 3962
Size: 1156 4749
ApoI r'AATT_y
Cuts at: 1493 3214 3440 3592 5701 1493
Size: 1721 226 152 2109 1697
Fragments arranged by size:
2109 1721 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 2097 2615 2669 3230 1870
Size: 73 154 518 54 561 4545
Fragments arranged by size:
4545 561 518 154 73 54
BamHI G'GATC_C
Cuts at: 3235 3602 3235
Size: 367 5538
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2064 2796 3171 3226 4681
Size: 325 217 627 277 732 375 55 1455
Cuts at: 4681 618
Size: 1842
Fragments arranged by size:
1842 1455 732 627 375 325 277 217
55
BanII G_rGCy'C
Cuts at: 1391 2143 2204 3224 1391
Size: 752 61 1020 4072
Fragments arranged by size:
4072 1020 752 61
BbeI G_GCGC'C
Cuts at: 2068 2068
Size: 5905
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2720 928
Size: 971 821 4113
Fragments arranged by size:
4113 971 821
BbvCI CC'TCA_GC
Cuts at: 2970 2976 3012 3030 2970
Size: 6 36 18 5845
Fragments arranged by size:
5845 36 18 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2335 2369 2767 2801 4096 4130 1903
Size: 34 398 34 398 34 1295 34 3678
Fragments arranged by size:
3678 1295 398 398 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3063 3797 5007 3063
Size: 734 1210 3961
Fragments arranged by size:
3961 1210 734
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5905
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2331 2375 2870
Size: 107 122 71 631 605 659 44 495
Cuts at: 2870 3169 4516 136
Size: 299 1347 1525
Fragments arranged by size:
1525 1347 659 631 605 495 299 122
107 71 44
BglII A'GATC_T
Cuts at: 5900 5900
Size: 5905
BlpI GC'TnA_GC
Cuts at: 3322 3322
Size: 5905
Bme1580I G_kGCm'C
Cuts at: 1790 2143 2154 2801 3174 3966 5122 1790
Size: 353 11 647 373 792 1156 2573
Fragments arranged by size:
2573 1156 792 647 373 353 11
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5905
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2640 3636 4594
Size: 416 53 83 399 24 1223 996 958
Cuts at: 4594 442
Size: 1753
Fragments arranged by size:
1753 1223 996 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2453 2492 2936 4565 1239
Size: 1214 39 444 1629 2579
Fragments arranged by size:
2579 1629 1214 444 39
Bpu10I CC'TnA_GC
Cuts at: 2970 2976 3012 3030 2970
Size: 6 36 18 5845
Fragments arranged by size:
5845 36 18 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2672 4030 4895 5193 2672
Size: 1358 865 298 3384
Fragments arranged by size:
3384 1358 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4568 882
Size: 617 3069 2219
Fragments arranged by size:
3069 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3696 493
Size: 711 2492 2702
Fragments arranged by size:
2702 2492 711
BsaBI GATnn'nnATC
Cuts at: 5899 5899
Size: 5905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2065 3712 4094 4729
Size: 53 83 186 936 532 1647 382 635
Cuts at: 4729 275
Size: 1451
Fragments arranged by size:
1647 1451 936 635 532 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4338 4736 5010 5157 5568 4338
Size: 398 274 147 411 4675
Fragments arranged by size:
4675 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5680
Fragments arranged by size:
5680 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2282 1186
Size: 1096 4809
BseYI C'CCAG_C
Cuts at: 2041 2086 2328 2420 2964 2988 3006 3036 5108
Size: 45 242 92 544 24 18 30 2072
Cuts at: 5108 5604 2041
Size: 496 2342
Fragments arranged by size:
2342 2072 544 496 242 92 45 30
24 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2681 2864 1163
Size: 573 945 183 4204
Fragments arranged by size:
4204 945 573 183
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2317 2631 3355 4116 5144 1540
Size: 275 186 316 314 724 761 1028 2301
Fragments arranged by size:
2301 1028 761 724 316 314 275 186
BsiHKAI G_wGCw'C
Cuts at: 2204 3966 4051 5122 2204
Size: 1762 85 1071 2987
Fragments arranged by size:
2987 1762 1071 85
BsmI GAATG_Cn'
Cuts at: 3416 3509 3416
Size: 93 5812
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2143 2154 2204 2801 3174 3224 3966
Size: 399 353 11 50 597 373 50 742
Cuts at: 3966 4051 5122 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 597 399 373 353 85
50 50 11
BspEI T'CCGG_A
Cuts at: 5568 5568
Size: 5905
BspHI T'CATG_A
Cuts at: 3794 5524 3794
Size: 1730 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3260 844
Size: 2416 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2269 3792 1394
Size: 429 446 1523 3507
Fragments arranged by size:
3507 1523 446 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4400 4574 65
Size: 1183 3152 174 1396
Fragments arranged by size:
3152 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2017 2626 2677 3174 4549 4736 1700
Size: 110 207 609 51 497 1375 187 2869
Fragments arranged by size:
2869 1375 609 497 207 187 110 51
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5905
BssHII G'CGCG_C
Cuts at: 1925 2298 1925
Size: 373 5532
BssSI C'ACGA_G
Cuts at: 3965 4977 3965
Size: 1012 4893
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2842 794
Size: 2048 3857
BstBI TT'CG_AA
Cuts at: 4709 4709
Size: 5905
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5905
BstXI CCAn_nnnn'nTGG
Cuts at: 2648 2648
Size: 5905
BstYI r'GATC_y
Cuts at: 1972 3235 3602 3987 4004 5456 5542 5554 5900
Size: 1263 367 385 17 1452 86 12 346
Cuts at: 5900 1972
Size: 1977
Fragments arranged by size:
1977 1452 1263 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2054 2317 2718 513
Size: 552 989 263 401 3700
Fragments arranged by size:
3700 989 552 401 263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5166
Fragments arranged by size:
5166 683 56
BtsI GCAGTG_nn'
Cuts at: 3421 4215 4235 3421
Size: 794 20 5091
Fragments arranged by size:
5091 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 3209 3209
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2233 4912 809
Size: 507 917 2679 1802
Fragments arranged by size:
2679 1802 917 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2144 2314 2628
Size: 54 1425 50 275 18 314 170 314
Cuts at: 2628 3352 4241 5870 8
Size: 724 889 1629 43
Fragments arranged by size:
1629 1425 889 724 314 314 275 170
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2314 2628 3352 1537
Size: 275 502 314 724 4090
Fragments arranged by size:
4090 724 502 314 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3835 1353
Size: 2482 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4492 4866 5012 387
Size: 1064 138 2903 374 146 1280
Fragments arranged by size:
2903 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2202 2202
Size: 5905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2140 2453 2477 2492 2936 3968 4565
Size: 393 508 313 24 15 444 1032 597
Cuts at: 4565 5352 5620 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1032 787 597 508 444 393 313
268 24 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2528 2528
Size: 5905
EcoO109I rG'GnC_Cy
Cuts at: 2139 2208 2248 2139
Size: 69 40 5796
Fragments arranged by size:
5796 69 40
EcoRI G'AATT_C
Cuts at: 3214 3592 3214
Size: 378 5527
FseI GG_CCGG'CC
Cuts at: 2630 2630
Size: 5905
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 2032 2068 2131 2167 2456 5052 1678
Size: 354 36 63 36 289 2596 2531
Fragments arranged by size:
2596 2531 354 289 63 36 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4546 4578 4620 4652 1373
Size: 32 135 32 2974 32 42 32 2626
Fragments arranged by size:
2974 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3249 3495 669
Size: 988 281 1311 246 3079
Fragments arranged by size:
3079 1311 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5905
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KasI G'GCGC_C
Cuts at: 2064 2064
Size: 5905
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2730 3219 5019 5203 1305
Size: 1425 489 1800 184 2007
Fragments arranged by size:
2007 1800 1425 489 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4083
Fragments arranged by size:
4083 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2762 3863 4222 4381 518
Size: 648 1596 1101 359 159 2042
Fragments arranged by size:
2042 1596 1101 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2056 2086 2328 2563 2657 3321 3998 5146
Size: 450 30 242 235 94 664 677 1148
Cuts at: 5146 5391 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 664 450 245 242 235
94 30
NaeI GCC'GGC
Cuts at: 2019 2628 2019
Size: 609 5296
NarI GG'CG_CC
Cuts at: 2065 2065
Size: 5905
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5353
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NgoMIV G'CCGG_C
Cuts at: 2017 2626 2017
Size: 609 5296
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 2752 3263 4808 1132
Size: 97 1523 511 1545 2229
Fragments arranged by size:
2229 1545 1523 511 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5905
PciI A'CATG_T
Cuts at: 2748 4804 2748
Size: 2056 3849
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5905
PfoI T'CCnGG_A
Cuts at: 2204 2204
Size: 5905
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 5487 5519 3974
Size: 32 1481 32 4360
Fragments arranged by size:
4360 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2208 2248 2208
Size: 40 5865
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PspOMI G'GGCC_C
Cuts at: 2139 2139
Size: 5905
PspXI vC'TCGA_Gb
Cuts at: 2669 2669
Size: 5905
PstI C_TGCA'G
Cuts at: 830 2838 3257 830
Size: 2008 419 3478
Fragments arranged by size:
3478 2008 419
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
PvuII CAG'CTG
Cuts at: 2563 2563
Size: 5905
SacI G_AGCT'C
Cuts at: 2204 2204
Size: 5905
SacII CC_GC'GG
Cuts at: 2057 2057
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 3247 1936
Size: 1311 4594
SanDI GG'GwC_CC
Cuts at: 2248 2248
Size: 5905
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SexAI A'CCwGG_T
Cuts at: 2904 2904
Size: 5905
SfcI C'TryA_G
Cuts at: 826 1045 2834 3253 4388 5069 5260 826
Size: 219 1789 419 1135 681 191 1471
Fragments arranged by size:
1789 1471 1135 681 419 219 191
SfoI GGC'GCC
Cuts at: 2066 2066
Size: 5905
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5905
SmaI CCC'GGG
Cuts at: 1872 2617 3232 1872
Size: 745 615 4545
Fragments arranged by size:
4545 745 615
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2669 2687 4009 4910 5172 820
Size: 197 926 726 18 1322 901 262 1553
Fragments arranged by size:
1553 1322 926 901 726 262 197 18
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SrfI GCCC'GGGC
Cuts at: 2617 2617
Size: 5905
SspI AAT'ATT
Cuts at: 5 52 3829 5
Size: 47 3777 2081
Fragments arranged by size:
3777 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5905
StyI C'CwwG_G
Cuts at: 513 1065 3344 513
Size: 552 2279 3074
Fragments arranged by size:
3074 2279 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2078 3111 3153 3159 3932 3949 4102
Size: 1255 498 1033 42 6 773 17 153
Cuts at: 4102 4287 4732 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1033 773 498 445 185 153
42 17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2745 2751 2847
Size: 274 80 33 51 1443 768 6 96
Cuts at: 2847 4151 5863 96
Size: 1304 1712 138
Fragments arranged by size:
1712 1443 1304 768 274 138 96 80
51 33 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5865 5897 5865
Size: 32 5873
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5905
XbaI T'CTAG_A
Cuts at: 3241 3241
Size: 5905
XhoI C'TCGA_G
Cuts at: 1943 2669 1943
Size: 726 5179
XmaI C'CCGG_G
Cuts at: 1870 2615 3230 1870
Size: 745 615 4545
Fragments arranged by size:
4545 745 615
XmnI GAAnn'nnTTC
Cuts at: 4034 4034
Size: 5905
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3713 276
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FspAI NruI
NsiI PacI PmlI PsrI RsrII SapI SfiI SwaI
XcmI