Restriction map for
pFN21AB9858
(Circular) (Six-base) MAPSORT of: pFN21AB9858.seq Check: 3566 from: 1 to: 5905
pFN21AB9858 5905 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3715 278
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3248 1937
Size: 1311 4594
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3107 3968 5352 5620 1632
Size: 1475 861 1384 268 1917
Fragments arranged by size:
1917 1475 1384 861 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 4804 5781 1203
Size: 3601 977 1327
Fragments arranged by size:
3601 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 2669 4634 2669
Size: 1965 3940
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5905
AlwNI CAG_nnn'CTG
Cuts at: 2780 3170 3648 5220 2780
Size: 390 478 1572 3465
Fragments arranged by size:
3465 1572 478 390
ApaI G_GGCC'C
Cuts at: 2866 2866
Size: 5905
ApaLI G'TGCA_C
Cuts at: 3962 5118 3962
Size: 1156 4749
ApoI r'AATT_y
Cuts at: 1493 3214 3440 3592 5701 1493
Size: 1721 226 152 2109 1697
Fragments arranged by size:
2109 1721 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 3230 1870
Size: 73 1287 4545
Fragments arranged by size:
4545 1287 73
BamHI G'GATC_C
Cuts at: 2225 2381 3235 3602 2225
Size: 156 854 367 4528
Fragments arranged by size:
4528 854 367 156
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 2492 2546 2835 3226
Size: 325 217 627 462 243 54 289 391
Cuts at: 3226 4681 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 462 391 325 289 243
217 54
BanII G_rGCy'C
Cuts at: 1391 2121 2129 2452 2646 2866 2987 3068 3180
Size: 730 8 323 194 220 121 81 112
Cuts at: 3180 3224 1391
Size: 44 4072
Fragments arranged by size:
4072 730 323 220 194 121 112 81
44 8
BbeI G_GCGC'C
Cuts at: 2839 2839
Size: 5905
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4934
BbvCI CC'TCA_GC
Cuts at: 2191 2191
Size: 5905
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4096 4130 1903
Size: 34 2159 34 3678
Fragments arranged by size:
3678 2159 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3797 5007 3797
Size: 1210 4695
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5905
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2798 4516 136
Size: 107 122 71 631 605 1126 1718 1525
Fragments arranged by size:
1718 1525 1126 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5900 5900
Size: 5905
BlpI GC'TnA_GC
Cuts at: 2873 3322 2873
Size: 449 5456
Bme1580I G_kGCm'C
Cuts at: 1790 2322 2736 2866 3966 5122 1790
Size: 532 414 130 1100 1156 2573
Fragments arranged by size:
2573 1156 1100 532 414 130
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5905
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2187 2607 2868
Size: 416 53 83 399 24 770 420 261
Cuts at: 2868 3636 4594 442
Size: 768 958 1753
Fragments arranged by size:
1753 958 770 768 420 416 399 261
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2472 1056
Size: 1416 4489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2406 2416 2470 2498 2552 2606 2632 2660
Size: 1167 10 54 28 54 54 26 28
Cuts at: 2660 4565 1239
Size: 1905 2579
Fragments arranged by size:
2579 1905 1167 54 54 54 28 28
26 10
Bpu10I CC'TnA_GC
Cuts at: 2123 2191 2274 2123
Size: 68 83 5754
Fragments arranged by size:
5754 83 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4030 4895 5193 4030
Size: 865 298 4742
Fragments arranged by size:
4742 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2110 2474 2582 2677 4568 882
Size: 617 611 364 108 95 1891 2219
Fragments arranged by size:
2219 1891 617 611 364 108 95
BsaAI yAC'GTr
Cuts at: 493 1204 3696 493
Size: 711 2492 2702
Fragments arranged by size:
2702 2492 711
BsaBI GATnn'nnATC
Cuts at: 5899 5899
Size: 5905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2836 2907 3159 3712
Size: 53 83 186 936 1303 71 252 553
Cuts at: 3712 4094 4729 275
Size: 382 635 1451
Fragments arranged by size:
1451 1303 936 635 553 382 252 186
83 71 53
BsaWI w'CCGG_w
Cuts at: 2090 2739 4338 4736 5010 5157 5568 2090
Size: 649 1599 398 274 147 411 2427
Fragments arranged by size:
2427 1599 649 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2404 2434 2439 2469 3074
Size: 30 165 30 1001 30 5 30 605
Cuts at: 3074 3104 1178
Size: 30 3979
Fragments arranged by size:
3979 1001 605 165 30 30 30 30
30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2951 3095 3134 3137 1186
Size: 1765 144 39 3 3954
Fragments arranged by size:
3954 1765 144 39 3
BseYI C'CCAG_C
Cuts at: 2915 3104 3163 5108 5604 2915
Size: 189 59 1945 496 3216
Fragments arranged by size:
3216 1945 496 189 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2096 1163
Size: 573 360 4972
Fragments arranged by size:
4972 573 360
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3355 4116 5144 1540
Size: 275 186 1354 761 1028 2301
Fragments arranged by size:
2301 1354 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2066 2129 3966 4051 5122 2066
Size: 63 1837 85 1071 2849
Fragments arranged by size:
2849 1837 1071 85 63
BsmI GAATG_Cn'
Cuts at: 3416 3509 3416
Size: 93 5812
BsmBI CGTCTCn'nnnn_
Cuts at: 3183 3183
Size: 5905
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2121 2129 2322 2452 2646 2736
Size: 399 276 55 8 193 130 194 90
Cuts at: 2736 2866 2987 3068 3180 3224 3966 4051 5122
Size: 130 121 81 112 44 742 85 1071
Cuts at: 5122 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 276 194 193 130
130 121 112 90 85 81 55 44
8
BspEI T'CCGG_A
Cuts at: 2090 2739 5568 2090
Size: 649 2829 2427
Fragments arranged by size:
2829 2427 649
BspHI T'CATG_A
Cuts at: 3794 5524 3794
Size: 1730 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3260 844
Size: 2416 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3792 1394
Size: 429 1969 3507
Fragments arranged by size:
3507 1969 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4400 4574 65
Size: 1183 3152 174 1396
Fragments arranged by size:
3152 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2838 4549 4736 1700
Size: 110 1028 1711 187 2869
Fragments arranged by size:
2869 1711 1028 187 110
BsrGI T'GTAC_A
Cuts at: 96 2136 96
Size: 2040 3865
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5905
BssSI C'ACGA_G
Cuts at: 2760 2813 2867 3965 4977 2760
Size: 53 54 1098 1012 3688
Fragments arranged by size:
3688 1098 1012 54 53
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5905
BstBI TT'CG_AA
Cuts at: 4709 4709
Size: 5905
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5905
BstYI r'GATC_y
Cuts at: 1972 2225 2381 3235 3602 3987 4004 5456 5542
Size: 253 156 854 367 385 17 1452 86
Cuts at: 5542 5554 5900 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 854 385 367 346 253 156
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2163 2934 2163
Size: 771 5134
BtgI C'CryG_G
Cuts at: 513 1065 2004 2460 2514 2568 2958 513
Size: 552 939 456 54 54 390 3460
Fragments arranged by size:
3460 939 552 456 390 54 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3089 487
Size: 683 56 1863 3303
Fragments arranged by size:
3303 1863 683 56
BtsI GCAGTG_nn'
Cuts at: 2079 3421 4215 4235 2079
Size: 1342 794 20 3749
Fragments arranged by size:
3749 1342 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3022 3057 529
Size: 35 2458 35 3377
Fragments arranged by size:
3377 2458 35 35
DraI TTT'AAA
Cuts at: 3209 3209
Size: 5905
DraIII CAC_nnn'GTG
Cuts at: 2392 2392
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4912 809
Size: 507 3596 1802
Fragments arranged by size:
3596 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3135 3352
Size: 54 1425 50 275 18 177 1128 217
Cuts at: 3352 4241 5870 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1128 889 275 217 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3352 1537
Size: 275 1540 4090
Fragments arranged by size:
4090 1540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3835 1353
Size: 2482 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2869 4492 4866 5012 387
Size: 1064 138 1280 1623 374 146 1280
Fragments arranged by size:
1623 1280 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2127 2127
Size: 5905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2406 2416 2470 2498 2552 2606 2632
Size: 393 774 10 54 28 54 54 26
Cuts at: 2632 2660 3107 3968 4565 5352 5620 1239
Size: 28 447 861 597 787 268 1524
Fragments arranged by size:
1524 861 787 774 597 447 393 268
54 54 54 28 28 26 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2434 2434
Size: 5905
EcoO109I rG'GnC_Cy
Cuts at: 2862 2863 2929 3101 2862
Size: 1 66 172 5666
Fragments arranged by size:
5666 172 66 1
EcoRI G'AATT_C
Cuts at: 3214 3592 3214
Size: 378 5527
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 2839 5052 1678
Size: 1161 2213 2531
Fragments arranged by size:
2531 2213 1161
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2174 2206 2655 2687 2741
Size: 32 135 32 602 32 449 32 54
Cuts at: 2741 2773 2969 3001 3074 3106 4546 4578 4620
Size: 32 196 32 73 32 1440 32 42
Cuts at: 4620 4652 1373
Size: 32 2626
Fragments arranged by size:
2626 1440 602 449 196 135 73 54
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3249 3495 669
Size: 988 281 1311 246 3079
Fragments arranged by size:
3079 1311 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5905
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KasI G'GCGC_C
Cuts at: 2835 2835
Size: 5905
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2729 5019 5203 1305
Size: 1424 2290 184 2007
Fragments arranged by size:
2290 2007 1424 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 3137 10
Size: 54 1425 343 177 1128 2778
Fragments arranged by size:
2778 1425 1128 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2306 3863 4222 4381 518
Size: 648 1140 1557 359 159 2042
Fragments arranged by size:
2042 1557 1140 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2652 3321 3998 5146 5391 1606
Size: 1046 669 677 1148 245 2120
Fragments arranged by size:
2120 1148 1046 677 669 245
NaeI GCC'GGC
Cuts at: 2840 2840
Size: 5905
NarI GG'CG_CC
Cuts at: 2836 2836
Size: 5905
NcoI C'CATG_G
Cuts at: 513 1065 2004 2514 2568 513
Size: 552 939 510 54 3850
Fragments arranged by size:
3850 939 552 510 54
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NgoMIV G'CCGG_C
Cuts at: 2838 2838
Size: 5905
NheI G'CTAG_C
Cuts at: 1052 2468 1052
Size: 1416 4489
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 2770 3263 4808 1132
Size: 97 1541 493 1545 2229
Fragments arranged by size:
2229 1545 1541 493 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5905
PciI A'CATG_T
Cuts at: 4804 4804
Size: 5905
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5905
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 5487 5519 3974
Size: 32 1481 32 4360
Fragments arranged by size:
4360 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2929 2929
Size: 5905
PshAI GACnn'nnGTC
Cuts at: 1613 3052 1613
Size: 1439 4466
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PspOMI G'GGCC_C
Cuts at: 2862 2862
Size: 5905
PstI C_TGCA'G
Cuts at: 830 3257 830
Size: 2427 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
SacI G_AGCT'C
Cuts at: 2129 2129
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 3247 1936
Size: 1311 4594
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SfcI C'TryA_G
Cuts at: 826 1045 3253 4388 5069 5260 826
Size: 219 2208 1135 681 191 1471
Fragments arranged by size:
2208 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2837 2837
Size: 5905
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 2838 1700
Size: 1138 4767
SmaI CCC'GGG
Cuts at: 1872 3232 1872
Size: 1360 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4009 4910 5172 820
Size: 197 926 2066 901 262 1553
Fragments arranged by size:
2066 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 2770 3263 1132
Size: 1638 493 3774
Fragments arranged by size:
3774 1638 493
SspI AAT'ATT
Cuts at: 5 52 3829 5
Size: 47 3777 2081
Fragments arranged by size:
3777 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5905
StyI C'CwwG_G
Cuts at: 513 1065 2004 2026 2514 2568 3344 513
Size: 552 939 22 488 54 776 3074
Fragments arranged by size:
3074 939 776 552 488 54 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2098 3932 3949 4102 4287 4732 325
Size: 1255 518 1834 17 153 185 445 1498
Fragments arranged by size:
1834 1498 1255 518 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2136 4151 5863
Size: 274 80 33 51 1443 159 2015 1712
Cuts at: 5863 96
Size: 138
Fragments arranged by size:
2015 1712 1443 274 159 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5865 5897 5865
Size: 32 5873
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5905
XbaI T'CTAG_A
Cuts at: 3241 3241
Size: 5905
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5905
XmaI C'CCGG_G
Cuts at: 1870 3230 1870
Size: 1360 4545
XmnI GAAnn'nnTTC
Cuts at: 4034 4034
Size: 5905
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3713 276
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI