Restriction map for
pFN21AB9856
(Circular) (Six-base) MAPSORT of: pFN21AB9856.seq Check: 7915 from: 1 to: 5875
pFN21AB9856 5875 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3685 278
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2634 3218 1937
Size: 697 584 4594
Fragments arranged by size:
4594 697 584
Acc65I G'GTAC_C
Cuts at: 3196 3196
Size: 5875
AclI AA'CG_TT
Cuts at: 1563 4002 4375 1563
Size: 2439 373 3063
Fragments arranged by size:
3063 2439 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3938 5322 5590 1632
Size: 2306 1384 268 1917
Fragments arranged by size:
2306 1917 1384 268
AfeI AGC'GCT
Cuts at: 2753 2753
Size: 5875
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5678
AflIII A'CryG_T
Cuts at: 1203 4774 5751 1203
Size: 3571 977 1327
Fragments arranged by size:
3571 1327 977
AgeI A'CCGG_T
Cuts at: 4706 4706
Size: 5875
AhdI GACnn_n'nnGTC
Cuts at: 4604 4604
Size: 5875
AleI CACnn'nnGTG
Cuts at: 1166 2424 1166
Size: 1258 4617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5875
AlwNI CAG_nnn'CTG
Cuts at: 3618 5190 3618
Size: 1572 4303
ApaLI G'TGCA_C
Cuts at: 3166 3932 5088 3166
Size: 766 1156 3953
Fragments arranged by size:
3953 1156 766
ApoI r'AATT_y
Cuts at: 1493 3184 3410 3562 5671 1493
Size: 1691 226 152 2109 1697
Fragments arranged by size:
2109 1697 1691 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4429 160
Size: 872 3397 1606
Fragments arranged by size:
3397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
AvaI C'yCGr_G
Cuts at: 1870 1943 2760 2786 3200 1870
Size: 73 817 26 414 4545
Fragments arranged by size:
4545 817 414 73 26
AvrII C'CTAG_G
Cuts at: 2950 2950
Size: 5875
BamHI G'GATC_C
Cuts at: 3205 3572 3205
Size: 367 5508
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3196 4651 618
Size: 325 217 627 1409 1455 1842
Fragments arranged by size:
1842 1455 1409 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2051 2767 3194 1391
Size: 660 716 427 4072
Fragments arranged by size:
4072 716 660 427
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2521 928
Size: 971 622 4282
Fragments arranged by size:
4282 971 622
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4066 4100 1903
Size: 34 2129 34 3678
Fragments arranged by size:
3678 2129 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3767 4977 3767
Size: 1210 4665
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2276 4486 136
Size: 107 122 71 631 605 604 2210 1525
Fragments arranged by size:
2210 1525 631 605 604 122 107 71
BglII A'GATC_T
Cuts at: 5870 5870
Size: 5875
BlpI GC'TnA_GC
Cuts at: 3292 3292
Size: 5875
Bme1580I G_kGCm'C
Cuts at: 1790 2838 3170 3936 5092 1790
Size: 1048 332 766 1156 2573
Fragments arranged by size:
2573 1156 1048 766 332
BmgBI CAC'GTC
Cuts at: 1318 2796 2833 1318
Size: 1478 37 4360
Fragments arranged by size:
4360 1478 37
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2363 2458 3606
Size: 416 53 83 399 24 946 95 1148
Cuts at: 3606 4564 442
Size: 958 1753
Fragments arranged by size:
1753 1148 958 946 416 399 95 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4535 1239
Size: 3296 2579
Bpu10I CC'TnA_GC
Cuts at: 2574 2574
Size: 5875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2052 4000 4865 5163 2052
Size: 1948 865 298 2764
Fragments arranged by size:
2764 1948 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2048 4538 882
Size: 617 549 2490 2219
Fragments arranged by size:
2490 2219 617 549
BsaAI yAC'GTr
Cuts at: 493 1204 2701 3666 493
Size: 711 1497 965 2702
Fragments arranged by size:
2702 1497 965 711
BsaBI GATnn'nnATC
Cuts at: 2315 3017 5869 2315
Size: 702 2852 2321
Fragments arranged by size:
2852 2321 702
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2463 2566 2732 2892
Size: 53 83 186 936 930 103 166 160
Cuts at: 2892 3682 4064 4699 275
Size: 790 382 635 1451
Fragments arranged by size:
1451 936 930 790 635 382 186 166
160 103 83 53
BsaWI w'CCGG_w
Cuts at: 2229 2846 4308 4706 4980 5127 5538 2229
Size: 617 1462 398 274 147 411 2566
Fragments arranged by size:
2566 1462 617 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2114 2120 2144 2150 1178
Size: 30 165 30 711 6 24 6 4903
Fragments arranged by size:
4903 711 165 30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2600 2663 1186
Size: 1414 63 4398
Fragments arranged by size:
4398 1414 63
BseYI C'CCAG_C
Cuts at: 2766 2837 3073 5078 5574 2766
Size: 71 236 2005 496 3067
Fragments arranged by size:
3067 2005 496 236 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2228 2402 1163
Size: 573 492 174 4636
Fragments arranged by size:
4636 573 492 174
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2344 3325 4086 5114 1540
Size: 275 186 343 981 761 1028 2301
Fragments arranged by size:
2301 1028 981 761 343 275 186
BsiHKAI G_wGCw'C
Cuts at: 2023 3170 3936 4021 5092 2023
Size: 1147 766 85 1071 2806
Fragments arranged by size:
2806 1147 1071 766 85
BsmI GAATG_Cn'
Cuts at: 2603 3140 3386 3479 2603
Size: 537 246 93 4999
Fragments arranged by size:
4999 537 246 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2051 2767 2838 3170 3194 3936
Size: 399 233 28 716 71 332 24 742
Cuts at: 3936 4021 5092 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 716 399 332 233 85
71 28 24
BspEI T'CCGG_A
Cuts at: 2846 5538 2846
Size: 2692 3183
BspHI T'CATG_A
Cuts at: 2508 3764 5494 2508
Size: 1256 1730 2889
Fragments arranged by size:
2889 1730 1256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2298 3230 844
Size: 1454 932 3489
Fragments arranged by size:
3489 1454 932
BsrBI CCG'CTC
Cuts at: 1394 1823 2712 3762 1394
Size: 429 889 1050 3507
Fragments arranged by size:
3507 1050 889 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4370 4544 65
Size: 1183 3122 174 1396
Fragments arranged by size:
3122 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2339 2744 4519 4706 1700
Size: 110 529 405 1775 187 2869
Fragments arranged by size:
2869 1775 529 405 187 110
BsrGI T'GTAC_A
Cuts at: 96 2328 96
Size: 2232 3643
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5875
BssSI C'ACGA_G
Cuts at: 3935 4947 3935
Size: 1012 4863
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5875
BstBI TT'CG_AA
Cuts at: 4679 4679
Size: 5875
BstEII G'GTnAC_C
Cuts at: 1184 2281 2803 1184
Size: 1097 522 4256
Fragments arranged by size:
4256 1097 522
BstYI r'GATC_y
Cuts at: 1972 2849 3205 3572 3957 3974 5426 5512 5524
Size: 877 356 367 385 17 1452 86 12
Cuts at: 5524 5870 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 877 385 367 356 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 2466 2640 513
Size: 552 939 39 423 174 3748
Fragments arranged by size:
3748 939 552 423 174 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2416 2662 487
Size: 683 56 1190 246 3700
Fragments arranged by size:
3700 1190 683 246 56
BtsI GCAGTG_nn'
Cuts at: 2403 3391 4185 4205 2403
Size: 988 794 20 4073
Fragments arranged by size:
4073 988 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5840
DraI TTT'AAA
Cuts at: 3133 3179 3133
Size: 46 5829
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4882 809
Size: 507 3566 1802
Fragments arranged by size:
3566 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2187 2341 2689
Size: 54 1425 50 275 18 357 154 348
Cuts at: 2689 2919 3322 4211 5840 8
Size: 230 403 889 1629 43
Fragments arranged by size:
1629 1425 889 403 357 348 275 230
154 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2341 3322 1537
Size: 275 529 981 4090
Fragments arranged by size:
4090 981 529 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2577 3805 1353
Size: 1224 1228 3423
Fragments arranged by size:
3423 1228 1224
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4462 4836 4982 387
Size: 1064 138 2873 374 146 1280
Fragments arranged by size:
2873 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3938 4535 5322 5590 1239
Size: 393 2306 597 787 268 1524
Fragments arranged by size:
2306 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3184 3562 3184
Size: 378 5497
FspI TGC'GCA
Cuts at: 4381 4381
Size: 5875
HaeII r_GCGC'y
Cuts at: 1678 2109 2177 2755 5022 1678
Size: 431 68 578 2267 2531
Fragments arranged by size:
2531 2267 578 431 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2120 2152 2257 2289 2576
Size: 32 135 32 548 32 105 32 287
Cuts at: 2576 2608 4516 4548 4590 4622 1373
Size: 32 1908 32 42 32 2626
Fragments arranged by size:
2626 1908 548 287 135 105 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3219 3465 669
Size: 988 281 1281 246 3079
Fragments arranged by size:
3079 1281 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5875
HpaI GTT'AAC
Cuts at: 3465 3465
Size: 5875
KpnI G_GTAC'C
Cuts at: 3200 3200
Size: 5875
MfeI C'AATT_G
Cuts at: 3474 3474
Size: 5875
MluI A'CGCG_T
Cuts at: 5751 5751
Size: 5875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2367 2574 2913 4989 5173 1305
Size: 1062 207 339 2076 184 2007
Fragments arranged by size:
2076 2007 1062 339 207 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2189 10
Size: 54 1425 343 357 3696
Fragments arranged by size:
3696 1425 357 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2306 2424 3833 4192 4351 518
Size: 648 1140 118 1409 359 159 2042
Fragments arranged by size:
2042 1409 1140 648 359 159 118
MspA1I CmG'CkG
Cuts at: 1606 2045 2346 2770 3291 3968 5116 5361 1606
Size: 439 301 424 521 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 521 439 424 301 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4384
Fragments arranged by size:
4384 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5875
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5875
NotI GC'GGCC_GC
Cuts at: 3322 3322
Size: 5875
NspI r_CATG'y
Cuts at: 1132 1229 3233 4778 1132
Size: 97 2004 1545 2229
Fragments arranged by size:
2229 2004 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5875
PciI A'CATG_T
Cuts at: 4774 4774
Size: 5875
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5875
PfoI T'CCnGG_A
Cuts at: 2714 2714
Size: 5875
PmeI GTTT'AAAC
Cuts at: 3179 3179
Size: 5875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3944 3976 5457 5489 3944
Size: 32 1481 32 4330
Fragments arranged by size:
4330 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5875
PsiI TTA'TAA
Cuts at: 3445 3445
Size: 5875
PstI C_TGCA'G
Cuts at: 830 2105 3227 830
Size: 1275 1122 3478
Fragments arranged by size:
3478 1275 1122
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5875
PvuII CAG'CTG
Cuts at: 2770 2770
Size: 5875
SacII CC_GC'GG
Cuts at: 2046 2046
Size: 5875
SalI G'TCGA_C
Cuts at: 1936 3217 1936
Size: 1281 4594
SbfI CC_TGCA'GG
Cuts at: 3227 3227
Size: 5875
ScaI AGT'ACT
Cuts at: 2154 2474 4123 5835 2154
Size: 320 1649 1712 2194
Fragments arranged by size:
2194 1712 1649 320
SfcI C'TryA_G
Cuts at: 826 1045 2101 3223 4358 5039 5230 826
Size: 219 1056 1122 1135 681 191 1471
Fragments arranged by size:
1471 1135 1122 1056 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5875
SmaI CCC'GGG
Cuts at: 1872 2788 3202 1872
Size: 916 414 4545
Fragments arranged by size:
4545 916 414
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2067 3979 4880 5142 820
Size: 197 926 124 1912 901 262 1553
Fragments arranged by size:
1912 1553 926 901 262 197 124
SnaBI TAC'GTA
Cuts at: 493 2701 3666 493
Size: 2208 965 2702
Fragments arranged by size:
2702 2208 965
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5875
SphI G_CATG'C
Cuts at: 1132 3233 1132
Size: 2101 3774
SspI AAT'ATT
Cuts at: 5 52 3799 5
Size: 47 3747 2081
Fragments arranged by size:
3747 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5875
StyI C'CwwG_G
Cuts at: 513 1065 2004 2109 2184 2950 3314 513
Size: 552 939 105 75 766 364 3074
Fragments arranged by size:
3074 939 766 552 364 105 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2636 2791 3902 3919 4072 4257 4702
Size: 1255 1056 155 1111 17 153 185 445
Cuts at: 4702 325
Size: 1498
Fragments arranged by size:
1498 1255 1111 1056 445 185 155 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2152 2328 2472
Size: 274 80 33 51 1443 175 176 144
Cuts at: 2472 4121 5833 96
Size: 1649 1712 138
Fragments arranged by size:
1712 1649 1443 274 176 175 144 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2841 2873 5835 5867 2841
Size: 32 2962 32 2849
Fragments arranged by size:
2962 2849 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5875
XbaI T'CTAG_A
Cuts at: 3211 3211
Size: 5875
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5875
XmaI C'CCGG_G
Cuts at: 1870 2786 3200 1870
Size: 916 414 4545
Fragments arranged by size:
4545 916 414
XmnI GAAnn'nnTTC
Cuts at: 4004 4004
Size: 5875
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3683 276
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI