Restriction map for
pFN21AB9851
(Circular) (Six-base) MAPSORT of: pFN21AB9851.seq Check: 8648 from: 1 to: 5845
pFN21AB9851 5845 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3655 278
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3188 1937
Size: 1251 4594
Acc65I G'GTAC_C
Cuts at: 3166 3166
Size: 5845
AclI AA'CG_TT
Cuts at: 1563 3972 4345 1563
Size: 2409 373 3063
Fragments arranged by size:
3063 2409 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2912 3042 3908 5292 5560 1632
Size: 1280 130 866 1384 268 1917
Fragments arranged by size:
1917 1384 1280 866 268 130
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5648
AflIII A'CryG_T
Cuts at: 1203 4744 5721 1203
Size: 3541 977 1327
Fragments arranged by size:
3541 1327 977
AgeI A'CCGG_T
Cuts at: 4676 4676
Size: 5845
AhdI GACnn_n'nnGTC
Cuts at: 2878 4574 2878
Size: 1696 4149
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5845
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2450 2585 1184
Size: 1266 135 4444
Fragments arranged by size:
4444 1266 135
AlwNI CAG_nnn'CTG
Cuts at: 2131 2650 2882 3588 5160 2131
Size: 519 232 706 1572 2816
Fragments arranged by size:
2816 1572 706 519 232
ApaI G_GGCC'C
Cuts at: 2733 2733
Size: 5845
ApaLI G'TGCA_C
Cuts at: 3902 5058 3902
Size: 1156 4689
ApoI r'AATT_y
Cuts at: 1493 2010 2378 2621 3154 3380 3532 5641 1493
Size: 517 368 243 533 226 152 2109 1697
Fragments arranged by size:
2109 1697 533 517 368 243 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4399 160
Size: 872 3367 1606
Fragments arranged by size:
3367 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
AvaI C'yCGr_G
Cuts at: 1870 1943 3170 1870
Size: 73 1227 4545
Fragments arranged by size:
4545 1227 73
BamHI G'GATC_C
Cuts at: 3089 3175 3542 3089
Size: 86 367 5392
Fragments arranged by size:
5392 367 86
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3166 4621 618
Size: 325 217 627 1379 1455 1842
Fragments arranged by size:
1842 1455 1379 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2733 3164 1391
Size: 1342 431 4072
Fragments arranged by size:
4072 1342 431
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4874
BbvCI CC'TCA_GC
Cuts at: 2319 2319
Size: 5845
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4036 4070 1903
Size: 34 2099 34 3678
Fragments arranged by size:
3678 2099 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2726 3737 4947 2726
Size: 1011 1210 3624
Fragments arranged by size:
3624 1210 1011
BclI T'GATC_A
Cuts at: 1548 2922 1548
Size: 1374 4471
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2448 3003 4456
Size: 107 122 71 631 605 776 555 1453
Cuts at: 4456 136
Size: 1525
Fragments arranged by size:
1525 1453 776 631 605 555 122 107
71
BglII A'GATC_T
Cuts at: 5840 5840
Size: 5845
BlpI GC'TnA_GC
Cuts at: 2438 3063 3262 2438
Size: 625 199 5021
Fragments arranged by size:
5021 625 199
Bme1580I G_kGCm'C
Cuts at: 1790 2733 3906 5062 1790
Size: 943 1173 1156 2573
Fragments arranged by size:
2573 1173 1156 943
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5845
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2043 2466 3123
Size: 416 53 83 399 24 626 423 657
Cuts at: 3123 3576 4534 442
Size: 453 958 1753
Fragments arranged by size:
1753 958 657 626 453 423 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4505 1239
Size: 3266 2579
Bpu10I CC'TnA_GC
Cuts at: 2319 2346 2630 2319
Size: 27 284 5534
Fragments arranged by size:
5534 284 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2264 3970 4835 5133 2264
Size: 1706 865 298 2976
Fragments arranged by size:
2976 1706 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2174 4508 882
Size: 617 675 2334 2219
Fragments arranged by size:
2334 2219 675 617
BsaAI yAC'GTr
Cuts at: 493 1204 3636 493
Size: 711 2432 2702
Fragments arranged by size:
2702 2432 711
BsaBI GATnn'nnATC
Cuts at: 5839 5839
Size: 5845
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3652 4034 4669 275
Size: 53 83 186 936 2119 382 635 1451
Fragments arranged by size:
2119 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4278 4676 4950 5097 5508 4278
Size: 398 274 147 411 4615
Fragments arranged by size:
4615 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2879 2909 1178
Size: 30 165 30 1476 30 4114
Fragments arranged by size:
4114 1476 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 2789 2972 1186
Size: 1000 603 183 4059
Fragments arranged by size:
4059 1000 603 183
BseYI C'CCAG_C
Cuts at: 2354 2650 5048 5544 2354
Size: 296 2398 496 2655
Fragments arranged by size:
2655 2398 496 296
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5272
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3295 4056 5084 1540
Size: 275 186 1294 761 1028 2301
Fragments arranged by size:
2301 1294 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3906 3991 5062 3906
Size: 85 1071 4689
Fragments arranged by size:
4689 1071 85
BsmI GAATG_Cn'
Cuts at: 3356 3449 3356
Size: 93 5752
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2733 3164 3906 3991 5062 1391
Size: 399 943 431 742 85 1071 2174
Fragments arranged by size:
2174 1071 943 742 431 399 85
BspEI T'CCGG_A
Cuts at: 5508 5508
Size: 5845
BspHI T'CATG_A
Cuts at: 3734 5464 3734
Size: 1730 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3200 844
Size: 2356 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2311 3732 1394
Size: 429 488 1421 3507
Fragments arranged by size:
3507 1421 488 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4340 4514 65
Size: 1183 3092 174 1396
Fragments arranged by size:
3092 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3044 4489 4676 1700
Size: 110 1234 1445 187 2869
Fragments arranged by size:
2869 1445 1234 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5845
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5845
BssSI C'ACGA_G
Cuts at: 3905 4917 3905
Size: 1012 4833
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5845
BstBI TT'CG_AA
Cuts at: 4649 4649
Size: 5845
BstEII G'GTnAC_C
Cuts at: 1184 3040 1184
Size: 1856 3989
BstXI CCAn_nnnn'nTGG
Cuts at: 2823 2823
Size: 5845
BstYI r'GATC_y
Cuts at: 1972 2677 3089 3175 3542 3927 3944 5396 5482
Size: 705 412 86 367 385 17 1452 86
Cuts at: 5482 5494 5840 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 705 412 385 367 346 86
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2862 2862
Size: 5845
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4354
Fragments arranged by size:
4354 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5106
Fragments arranged by size:
5106 683 56
BtsI GCAGTG_nn'
Cuts at: 2311 2704 2977 3361 4155 4175 2311
Size: 393 273 384 794 20 3981
Fragments arranged by size:
3981 794 393 384 273 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5845
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5810
DraI TTT'AAA
Cuts at: 3133 3149 3133
Size: 16 5829
DraIII CAC_nnn'GTG
Cuts at: 2474 2474
Size: 5845
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4852 809
Size: 507 3536 1802
Fragments arranged by size:
3536 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2086 2097
Size: 54 1425 50 275 18 192 64 11
Cuts at: 2097 2295 3292 4181 5810 8
Size: 198 997 889 1629 43
Fragments arranged by size:
1629 1425 997 889 275 198 192 64
54 50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 3292 1537
Size: 275 1480 4090
Fragments arranged by size:
4090 1480 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2855 2910 3061 3775 1353
Size: 1502 55 151 714 3423
Fragments arranged by size:
3423 1502 714 151 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4432 4806 4952 387
Size: 1064 138 2843 374 146 1280
Fragments arranged by size:
2843 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2912 3042 3908 4505 5292 5560 1239
Size: 393 1280 130 866 597 787 268 1524
Fragments arranged by size:
1524 1280 866 787 597 393 268 130
EcoNI CCTnn'n_nnAGG
Cuts at: 2504 2660 2504
Size: 156 5689
EcoRI G'AATT_C
Cuts at: 2010 2621 3154 3532 2010
Size: 611 533 378 4323
Fragments arranged by size:
4323 611 533 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 5845
FspI TGC'GCA
Cuts at: 4351 4351
Size: 5845
HaeII r_GCGC'y
Cuts at: 1678 4992 1678
Size: 3314 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2107 2139 2864 2896 4486
Size: 32 135 32 535 32 725 32 1590
Cuts at: 4486 4518 4560 4592 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1590 725 535 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3189 3435 669
Size: 988 281 1251 246 3079
Fragments arranged by size:
3079 1251 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5845
HpaI GTT'AAC
Cuts at: 3435 3435
Size: 5845
KpnI G_GTAC'C
Cuts at: 3170 3170
Size: 5845
MfeI C'AATT_G
Cuts at: 3444 3444
Size: 5845
MluI A'CGCG_T
Cuts at: 5721 5721
Size: 5845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4959 5143 1305
Size: 3654 184 2007
Fragments arranged by size:
3654 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2024 2099 2297 10
Size: 54 1425 343 192 75 198 3558
Fragments arranged by size:
3558 1425 343 198 192 75 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 2418 3803 4162 4321 518
Size: 648 1056 196 1385 359 159 2042
Fragments arranged by size:
2042 1385 1056 648 359 196 159
MspA1I CmG'CkG
Cuts at: 1606 2335 2490 3261 3938 5086 5331 1606
Size: 729 155 771 677 1148 245 2120
Fragments arranged by size:
2120 1148 771 729 677 245 155
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4354
Fragments arranged by size:
4354 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5845
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5845
NotI GC'GGCC_GC
Cuts at: 3292 3292
Size: 5845
NspI r_CATG'y
Cuts at: 1132 1229 2206 3203 4748 1132
Size: 97 977 997 1545 2229
Fragments arranged by size:
2229 1545 997 977 97
PasI CC'CwG_GG
Cuts at: 1348 3013 1348
Size: 1665 4180
PciI A'CATG_T
Cuts at: 4744 4744
Size: 5845
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2197 1108
Size: 1089 4756
PfoI T'CCnGG_A
Cuts at: 2058 2616 2058
Size: 558 5287
PmeI GTTT'AAAC
Cuts at: 3149 3149
Size: 5845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3946 5427 5459 3914
Size: 32 1481 32 4300
Fragments arranged by size:
4300 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5845
PsiI TTA'TAA
Cuts at: 3415 3415
Size: 5845
PspOMI G'GGCC_C
Cuts at: 2729 2729
Size: 5845
PstI C_TGCA'G
Cuts at: 830 2166 2340 2646 3197 830
Size: 1336 174 306 551 3478
Fragments arranged by size:
3478 1336 551 306 174
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5845
PvuII CAG'CTG
Cuts at: 2335 2490 2335
Size: 155 5690
SalI G'TCGA_C
Cuts at: 1936 3187 1936
Size: 1251 4594
SapI GCTCTTCn'nnn_
Cuts at: 2855 2910 2855
Size: 55 5790
SbfI CC_TGCA'GG
Cuts at: 3197 3197
Size: 5845
ScaI AGT'ACT
Cuts at: 4093 5805 4093
Size: 1712 4133
SfcI C'TryA_G
Cuts at: 826 1045 2162 2336 2642 3193 4328 5009 5200
Size: 219 1117 174 306 551 1135 681 191
Cuts at: 5200 826
Size: 1471
Fragments arranged by size:
1471 1135 1117 681 551 306 219 191
174
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5845
SmaI CCC'GGG
Cuts at: 1872 3172 1872
Size: 1300 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2279 3949 4850 5112 820
Size: 197 926 336 1670 901 262 1553
Fragments arranged by size:
1670 1553 926 901 336 262 197
SnaBI TAC'GTA
Cuts at: 493 3636 493
Size: 3143 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5845
SphI G_CATG'C
Cuts at: 1132 3203 1132
Size: 2071 3774
SspI AAT'ATT
Cuts at: 5 52 3769 5
Size: 47 3717 2081
Fragments arranged by size:
3717 2081 47
StuI AGG'CCT
Cuts at: 1517 2019 1517
Size: 502 5343
StyI C'CwwG_G
Cuts at: 513 1065 2004 3001 3084 3284 513
Size: 552 939 997 83 200 3074
Fragments arranged by size:
3074 997 939 552 200 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2485 3026 3872 3889 4042 4227 4672
Size: 1255 905 541 846 17 153 185 445
Cuts at: 4672 325
Size: 1498
Fragments arranged by size:
1498 1255 905 846 541 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4091 5803 96
Size: 274 80 33 51 1443 2114 1712 138
Fragments arranged by size:
2114 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2879 2911 5805 5837 2879
Size: 32 2894 32 2887
Fragments arranged by size:
2894 2887 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5845
XbaI T'CTAG_A
Cuts at: 3181 3181
Size: 5845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2194 2224 2740 2194
Size: 30 516 5299
Fragments arranged by size:
5299 516 30
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5845
XmaI C'CCGG_G
Cuts at: 1870 3170 1870
Size: 1300 4545
XmnI GAAnn'nnTTC
Cuts at: 3974 3974
Size: 5845
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3653 276
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I EcoICRI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI