Restriction map for
pFN21AB9848
(Circular) (Six-base) MAPSORT of: pFN21AB9848.seq Check: 9894 from: 1 to: 5833
pFN21AB9848 5833 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2835 2835
Size: 5833
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2810 3643 278
Size: 53 83 186 936 1274 833 2468
Fragments arranged by size:
2468 1274 936 833 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3176 1937
Size: 1239 4594
Acc65I G'GTAC_C
Cuts at: 3154 3154
Size: 5833
AclI AA'CG_TT
Cuts at: 1563 3132 3960 4333 1563
Size: 1569 828 373 3063
Fragments arranged by size:
3063 1569 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2432 2837 3896 5280 5548 1632
Size: 800 405 1059 1384 268 1917
Fragments arranged by size:
1917 1384 1059 800 405 268
AfeI AGC'GCT
Cuts at: 2885 2885
Size: 5833
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5636
AflIII A'CryG_T
Cuts at: 1203 2301 4732 5709 1203
Size: 1098 2431 977 1327
Fragments arranged by size:
2431 1327 1098 977
AgeI A'CCGG_T
Cuts at: 4664 4664
Size: 5833
AhdI GACnn_n'nnGTC
Cuts at: 2685 4562 2685
Size: 1877 3956
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5833
AlwNI CAG_nnn'CTG
Cuts at: 3576 5148 3576
Size: 1572 4261
ApaI G_GGCC'C
Cuts at: 2180 2276 2739 2180
Size: 96 463 5274
Fragments arranged by size:
5274 463 96
ApaLI G'TGCA_C
Cuts at: 3890 5046 3890
Size: 1156 4677
ApoI r'AATT_y
Cuts at: 1493 3142 3368 3520 5629 1493
Size: 1649 226 152 2109 1697
Fragments arranged by size:
2109 1697 1649 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4387 160
Size: 872 3355 1606
Fragments arranged by size:
3355 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 2269 2437 2779 3046 3083 3158 1870
Size: 73 326 168 342 267 37 75 4545
Fragments arranged by size:
4545 342 326 267 168 75 73 37
AvrII C'CTAG_G
Cuts at: 2551 2551
Size: 5833
BamHI G'GATC_C
Cuts at: 3163 3530 3163
Size: 367 5466
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2381 2659 2933 3017 3154
Size: 325 217 627 594 278 274 84 137
Cuts at: 3154 4609 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 594 325 278 274 217
137 84
BanII G_rGCy'C
Cuts at: 1391 2117 2180 2276 2728 2739 3081 3152 1391
Size: 726 63 96 452 11 342 71 4072
Fragments arranged by size:
4072 726 452 342 96 71 63 11
BbeI G_GCGC'C
Cuts at: 3021 3021
Size: 5833
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2962 928
Size: 971 1063 3799
Fragments arranged by size:
3799 1063 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4024 4058 1903
Size: 34 50 34 2003 34 3678
Fragments arranged by size:
3678 2003 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3725 4935 3725
Size: 1210 4623
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5833
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4444 136
Size: 107 122 71 631 605 2772 1525
Fragments arranged by size:
2772 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5828 5828
Size: 5833
BlpI GC'TnA_GC
Cuts at: 3250 3250
Size: 5833
Bme1580I G_kGCm'C
Cuts at: 1790 2180 2276 2384 2494 2664 2739 3894 5050
Size: 390 96 108 110 170 75 1155 1156
Cuts at: 5050 1790
Size: 2573
Fragments arranged by size:
2573 1156 1155 390 170 110 108 96
75
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5833
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2729 3564 4522
Size: 416 53 83 399 24 1312 835 958
Cuts at: 4522 442
Size: 1753
Fragments arranged by size:
1753 1312 958 835 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5833
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 5833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2172 2726 2903 4493 1239
Size: 933 554 177 1590 2579
Fragments arranged by size:
2579 1590 933 554 177
Bpu10I CC'TnA_GC
Cuts at: 2571 2671 2571
Size: 100 5733
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2586 3958 4823 5121 2586
Size: 1372 865 298 3298
Fragments arranged by size:
3298 1372 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2469 4496 882
Size: 617 970 2027 2219
Fragments arranged by size:
2219 2027 970 617
BsaAI yAC'GTr
Cuts at: 493 1204 3624 493
Size: 711 2420 2702
Fragments arranged by size:
2702 2420 711
BsaBI GATnn'nnATC
Cuts at: 5827 5827
Size: 5833
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2807 3018 3640 4022
Size: 53 83 186 936 1274 211 622 382
Cuts at: 4022 4657 275
Size: 635 1451
Fragments arranged by size:
1451 1274 936 635 622 382 211 186
83 53
BsaWI w'CCGG_w
Cuts at: 4266 4664 4938 5085 5496 4266
Size: 398 274 147 411 4603
Fragments arranged by size:
4603 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2120 2150 1178
Size: 30 165 30 717 30 4861
Fragments arranged by size:
4861 717 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2212 3041 1186
Size: 1026 829 3978
Fragments arranged by size:
3978 1026 829
BseYI C'CCAG_C
Cuts at: 2173 3035 5036 5532 2173
Size: 862 2001 496 2474
Fragments arranged by size:
2474 2001 862 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2973 1163
Size: 573 1237 4023
Fragments arranged by size:
4023 1237 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2657 2848 3283 4044 5072 1540
Size: 275 186 656 191 435 761 1028 2301
Fragments arranged by size:
2301 1028 761 656 435 275 191 186
BsiHKAI G_wGCw'C
Cuts at: 3894 3979 5050 3894
Size: 85 1071 4677
Fragments arranged by size:
4677 1071 85
BsmI GAATG_Cn'
Cuts at: 3344 3437 3344
Size: 93 5740
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 2180 2276 2384 2494 2664 2728
Size: 399 327 63 96 108 110 170 64
Cuts at: 2728 2739 3081 3152 3894 3979 5050 1391
Size: 11 342 71 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 342 327 170 110
108 96 85 71 64 63 11
BspEI T'CCGG_A
Cuts at: 5496 5496
Size: 5833
BspHI T'CATG_A
Cuts at: 3722 5452 3722
Size: 1730 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2088 2243 2835 3188 844
Size: 1244 155 592 353 3489
Fragments arranged by size:
3489 1244 592 353 155
BsrBI CCG'CTC
Cuts at: 1394 1823 3720 1394
Size: 429 1897 3507
Fragments arranged by size:
3507 1897 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4328 4502 65
Size: 1183 3080 174 1396
Fragments arranged by size:
3080 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2930 3062 4477 4664 1700
Size: 110 1120 132 1415 187 2869
Fragments arranged by size:
2869 1415 1120 187 132 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5833
BssHII G'CGCG_C
Cuts at: 1925 2415 1925
Size: 490 5343
BssSI C'ACGA_G
Cuts at: 2110 2359 3011 3893 4905 2110
Size: 249 652 882 1012 3038
Fragments arranged by size:
3038 1012 882 652 249
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5833
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 2230 1184
Size: 1046 4787
BstXI CCAn_nnnn'nTGG
Cuts at: 2392 2704 2392
Size: 312 5521
BstYI r'GATC_y
Cuts at: 1972 3163 3530 3915 3932 5384 5470 5482 5828
Size: 1191 367 385 17 1452 86 12 346
Cuts at: 5828 1972
Size: 1977
Fragments arranged by size:
1977 1452 1191 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2118 2118
Size: 5833
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5281
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5094
Fragments arranged by size:
5094 683 56
BtsI GCAGTG_nn'
Cuts at: 3078 3349 4143 4163 3078
Size: 271 794 20 4748
Fragments arranged by size:
4748 794 271 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2468 2503 529
Size: 35 1904 35 3859
Fragments arranged by size:
3859 1904 35 35
DraI TTT'AAA
Cuts at: 3137 3137
Size: 5833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4840 809
Size: 507 3524 1802
Fragments arranged by size:
3524 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2431 2991 3280
Size: 54 1425 50 275 18 601 560 289
Cuts at: 3280 4169 5798 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 601 560 289 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3280 1537
Size: 275 1468 4090
Fragments arranged by size:
4090 1468 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2876 3763 1353
Size: 1523 887 3423
Fragments arranged by size:
3423 1523 887
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2726 4420 4794 4940 387
Size: 1064 138 1137 1694 374 146 1280
Fragments arranged by size:
1694 1280 1137 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2172 2432 2726 2837 2903 3896 4493
Size: 393 540 260 294 111 66 993 597
Cuts at: 4493 5280 5548 1239
Size: 787 268 1524
Fragments arranged by size:
1524 993 787 597 540 393 294 268
260 111 66
EcoO109I rG'GnC_Cy
Cuts at: 2217 2242 2273 2368 2217
Size: 25 31 95 5682
Fragments arranged by size:
5682 95 31 25
EcoRI G'AATT_C
Cuts at: 3142 3520 3142
Size: 378 5455
FspI TGC'GCA
Cuts at: 2514 2978 4339 2514
Size: 464 1361 4008
Fragments arranged by size:
4008 1361 464
FspAI rTGC'GCAy
Cuts at: 2514 2514
Size: 5833
HaeII r_GCGC'y
Cuts at: 1678 2054 2447 2887 2914 3021 4980 1678
Size: 376 393 440 27 107 1959 2531
Fragments arranged by size:
2531 1959 440 393 376 107 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2120 2152 2671 2703 4474
Size: 32 135 32 548 32 519 32 1771
Cuts at: 4474 4506 4548 4580 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1771 548 519 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3177 3423 669
Size: 988 281 1239 246 3079
Fragments arranged by size:
3079 1239 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5833
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KasI G'GCGC_C
Cuts at: 3017 3017
Size: 5833
KpnI G_GTAC'C
Cuts at: 3158 3158
Size: 5833
MfeI C'AATT_G
Cuts at: 3432 3432
Size: 5833
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2452 3153 4947 5131 1305
Size: 1147 701 1794 184 2007
Fragments arranged by size:
2007 1794 1147 701 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2433 2993 10
Size: 54 1425 343 601 560 2850
Fragments arranged by size:
2850 1425 601 560 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2313 2508 3791 4150 4309 518
Size: 648 1147 195 1283 359 159 2042
Fragments arranged by size:
2042 1283 1147 648 359 195 159
MspA1I CmG'CkG
Cuts at: 1606 2015 2143 2173 2388 2531 2841 3249 3926
Size: 409 128 30 215 143 310 408 677
Cuts at: 3926 5074 5319 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 409 408 310 245 215
143 128 30
NaeI GCC'GGC
Cuts at: 3064 3064
Size: 5833
NarI GG'CG_CC
Cuts at: 3018 3018
Size: 5833
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5281
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5833
NgoMIV G'CCGG_C
Cuts at: 3062 3062
Size: 5833
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5833
NotI GC'GGCC_GC
Cuts at: 3280 3280
Size: 5833
NspI r_CATG'y
Cuts at: 1132 1229 2305 2513 3191 4736 1132
Size: 97 1076 208 678 1545 2229
Fragments arranged by size:
2229 1545 1076 678 208 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5833
PciI A'CATG_T
Cuts at: 2301 4732 2301
Size: 2431 3402
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5833
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 5415 5447 3902
Size: 32 1481 32 4288
Fragments arranged by size:
4288 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2242 2368 2242
Size: 126 5707
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5833
PsiI TTA'TAA
Cuts at: 3403 3403
Size: 5833
PspOMI G'GGCC_C
Cuts at: 2176 2272 2735 2176
Size: 96 463 5274
Fragments arranged by size:
5274 463 96
PstI C_TGCA'G
Cuts at: 830 2085 2148 2163 2295 2910 2963 3185 830
Size: 1255 63 15 132 615 53 222 3478
Fragments arranged by size:
3478 1255 615 222 132 63 53 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
PvuII CAG'CTG
Cuts at: 2388 2531 2388
Size: 143 5690
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SapI GCTCTTCn'nnn_
Cuts at: 2876 2876
Size: 5833
SbfI CC_TGCA'GG
Cuts at: 3185 3185
Size: 5833
ScaI AGT'ACT
Cuts at: 4081 5793 4081
Size: 1712 4121
SfcI C'TryA_G
Cuts at: 826 1045 2081 2144 2159 2291 2906 2959 3181
Size: 219 1036 63 15 132 615 53 222
Cuts at: 3181 4316 4997 5188 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1036 681 615 222 219 191
132 63 53 15
SfoI GGC'GCC
Cuts at: 3019 3019
Size: 5833
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5833
SmaI CCC'GGG
Cuts at: 1872 2271 2439 2781 3085 3160 1872
Size: 399 168 342 304 75 4545
Fragments arranged by size:
4545 399 342 304 168 75
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2601 3937 4838 5100 820
Size: 197 926 658 1336 901 262 1553
Fragments arranged by size:
1553 1336 926 901 658 262 197
SnaBI TAC'GTA
Cuts at: 493 3624 493
Size: 3131 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5833
SphI G_CATG'C
Cuts at: 1132 2513 3191 1132
Size: 1381 678 3774
Fragments arranged by size:
3774 1381 678
SspI AAT'ATT
Cuts at: 5 52 3757 5
Size: 47 3705 2081
Fragments arranged by size:
3705 2081 47
StuI AGG'CCT
Cuts at: 1517 2576 1517
Size: 1059 4774
StyI C'CwwG_G
Cuts at: 513 1065 2262 2551 2577 3021 3272 513
Size: 552 1197 289 26 444 251 3074
Fragments arranged by size:
3074 1197 552 444 289 251 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2355 3860 3877 4030 4215 4660 325
Size: 1255 775 1505 17 153 185 445 1498
Fragments arranged by size:
1505 1498 1255 775 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4079 5791 96
Size: 274 80 33 51 1443 2102 1712 138
Fragments arranged by size:
2102 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2345 2377 5793 5825 2345
Size: 32 3416 32 2353
Fragments arranged by size:
3416 2353 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5833
XbaI T'CTAG_A
Cuts at: 3169 3169
Size: 5833
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 2269 2437 2779 3083 3158 1870
Size: 399 168 342 304 75 4545
Fragments arranged by size:
4545 399 342 304 168 75
XmnI GAAnn'nnTTC
Cuts at: 3962 3962
Size: 5833
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2808 3641 276
Size: 53 83 186 936 1274 833 2468
Fragments arranged by size:
2468 1274 936 833 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I
DraIII EcoICRI EcoNI EcoRV FalI FseI NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SrfI SwaI XcmI