Restriction map for
pFN21AB9847
(Circular) (Six-base) MAPSORT of: pFN21AB9847.seq Check: 2218 from: 1 to: 5830
pFN21AB9847 5830 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2957 2957
Size: 5830
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2304 3173 1937
Size: 367 869 4594
Fragments arranged by size:
4594 869 367
Acc65I G'GTAC_C
Cuts at: 3151 3151
Size: 5830
AclI AA'CG_TT
Cuts at: 1563 3957 4330 1563
Size: 2394 373 3063
Fragments arranged by size:
3063 2394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3893 5277 5545 1632
Size: 2261 1384 268 1917
Fragments arranged by size:
2261 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5633
AflIII A'CryG_T
Cuts at: 1203 4729 5706 1203
Size: 3526 977 1327
Fragments arranged by size:
3526 1327 977
AgeI A'CCGG_T
Cuts at: 4661 4661
Size: 5830
AhdI GACnn_n'nnGTC
Cuts at: 4559 4559
Size: 5830
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5830
AlwNI CAG_nnn'CTG
Cuts at: 2293 3573 5145 2293
Size: 1280 1572 2978
Fragments arranged by size:
2978 1572 1280
ApaLI G'TGCA_C
Cuts at: 3887 5043 3887
Size: 1156 4674
ApoI r'AATT_y
Cuts at: 1493 3139 3365 3517 5626 1493
Size: 1646 226 152 2109 1697
Fragments arranged by size:
2109 1697 1646 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4384 160
Size: 872 3352 1606
Fragments arranged by size:
3352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 3155 1870
Size: 73 1212 4545
Fragments arranged by size:
4545 1212 73
BamHI G'GATC_C
Cuts at: 3160 3527 3160
Size: 367 5463
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 2721 2756 2874 3151
Size: 325 217 627 291 643 35 118 277
Cuts at: 3151 4606 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 643 627 325 291 277 217
118 35
BanII G_rGCy'C
Cuts at: 1391 3149 1391
Size: 1758 4072
BbeI G_GCGC'C
Cuts at: 2725 2725
Size: 5830
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2122 2545 2874 928
Size: 971 223 423 329 3884
Fragments arranged by size:
3884 971 423 329 223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4021 4055 1903
Size: 34 2084 34 3678
Fragments arranged by size:
3678 2084 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3722 4932 3722
Size: 1210 4620
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2252 4441 136
Size: 107 122 71 631 605 580 2189 1525
Fragments arranged by size:
2189 1525 631 605 580 122 107 71
BglII A'GATC_T
Cuts at: 5825 5825
Size: 5830
BlpI GC'TnA_GC
Cuts at: 3247 3247
Size: 5830
Bme1580I G_kGCm'C
Cuts at: 1790 2081 2759 2781 3891 5047 1790
Size: 291 678 22 1110 1156 2573
Fragments arranged by size:
2573 1156 1110 678 291 22
BmgBI CAC'GTC
Cuts at: 1318 2122 1318
Size: 804 5026
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2693 3561 4519
Size: 416 53 83 399 24 1276 868 958
Cuts at: 4519 442
Size: 1753
Fragments arranged by size:
1753 1276 958 868 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5830
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2357 2357
Size: 5830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2435 2564 2600 3062 4490 1239
Size: 1196 129 36 462 1428 2579
Fragments arranged by size:
2579 1428 1196 462 129 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3955 4820 5118 3955
Size: 865 298 4667
Fragments arranged by size:
4667 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2330 2389 4493 882
Size: 617 831 59 2104 2219
Fragments arranged by size:
2219 2104 831 617 59
BsaAI yAC'GTr
Cuts at: 493 1204 3621 493
Size: 711 2417 2702
Fragments arranged by size:
2702 2417 711
BsaBI GATnn'nnATC
Cuts at: 5824 5824
Size: 5830
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2722 3637 4019 4654
Size: 53 83 186 936 1189 915 382 635
Cuts at: 4654 275
Size: 1451
Fragments arranged by size:
1451 1189 936 915 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4263 4661 4935 5082 5493 4263
Size: 398 274 147 411 4600
Fragments arranged by size:
4600 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5605
Fragments arranged by size:
5605 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 2651 3059 1186
Size: 1084 381 408 3957
Fragments arranged by size:
3957 1084 408 381
BseYI C'CCAG_C
Cuts at: 2456 2774 5033 5529 2456
Size: 318 2259 496 2757
Fragments arranged by size:
2757 2259 496 318
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5257
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3280 4041 5069 1540
Size: 275 186 1279 761 1028 2301
Fragments arranged by size:
2301 1279 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2490 2835 3891 3976 5047 2490
Size: 345 1056 85 1071 3273
Fragments arranged by size:
3273 1071 1056 345 85
BsmI GAATG_Cn'
Cuts at: 3341 3434 3341
Size: 93 5737
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2490 2759 2781 2835 3149 3891
Size: 399 291 409 269 22 54 314 742
Cuts at: 3891 3976 5047 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 409 399 314 291 269
85 54 22
BspEI T'CCGG_A
Cuts at: 5493 5493
Size: 5830
BspHI T'CATG_A
Cuts at: 3719 5449 3719
Size: 1730 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2244 2779 2957 3105 3185 844
Size: 1400 535 178 148 80 3489
Fragments arranged by size:
3489 1400 535 178 148 80
BsrBI CCG'CTC
Cuts at: 1394 1823 3717 1394
Size: 429 1894 3507
Fragments arranged by size:
3507 1894 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2101 2499 4325 4499 65
Size: 1183 853 398 1826 174 1396
Fragments arranged by size:
1826 1396 1183 853 398 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2718 4474 4661 1700
Size: 110 908 1756 187 2869
Fragments arranged by size:
2869 1756 908 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5830
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5830
BssSI C'ACGA_G
Cuts at: 2405 3890 4902 2405
Size: 1485 1012 3333
Fragments arranged by size:
3333 1485 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5830
BstBI TT'CG_AA
Cuts at: 4634 4634
Size: 5830
BstEII G'GTnAC_C
Cuts at: 1184 2177 1184
Size: 993 4837
BstXI CCAn_nnnn'nTGG
Cuts at: 2448 2578 2448
Size: 130 5700
BstYI r'GATC_y
Cuts at: 1972 2325 3160 3527 3912 3929 5381 5467 5479
Size: 353 835 367 385 17 1452 86 12
Cuts at: 5479 5825 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 835 385 367 353 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3101 3101
Size: 5830
BtgI C'CryG_G
Cuts at: 513 1065 2115 2899 2937 513
Size: 552 1050 784 38 3406
Fragments arranged by size:
3406 1050 784 552 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5091
Fragments arranged by size:
5091 683 56
BtsI GCAGTG_nn'
Cuts at: 3346 4140 4160 3346
Size: 794 20 5016
Fragments arranged by size:
5016 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5795
DraI TTT'AAA
Cuts at: 3134 3134
Size: 5830
DraIII CAC_nnn'GTG
Cuts at: 2054 2054
Size: 5830
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2126 4837 809
Size: 507 810 2711 1802
Fragments arranged by size:
2711 1802 810 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2529 2618 3277
Size: 54 1425 50 275 18 699 89 659
Cuts at: 3277 4166 5795 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 699 659 275 89 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3277 1537
Size: 275 1465 4090
Fragments arranged by size:
4090 1465 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2669 3760 1353
Size: 1316 1091 3423
Fragments arranged by size:
3423 1316 1091
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2606 4417 4791 4937 387
Size: 1064 138 1017 1811 374 146 1280
Fragments arranged by size:
1811 1280 1064 1017 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2435 2564 2600 3062 3893 4490 5277
Size: 393 803 129 36 462 831 597 787
Cuts at: 5277 5545 1239
Size: 268 1524
Fragments arranged by size:
1524 831 803 787 597 462 393 268
129 36
EcoO109I rG'GnC_Cy
Cuts at: 2170 3007 3096 2170
Size: 837 89 4904
Fragments arranged by size:
4904 837 89
EcoRI G'AATT_C
Cuts at: 3139 3517 3139
Size: 378 5452
FspI TGC'GCA
Cuts at: 4336 4336
Size: 5830
HaeII r_GCGC'y
Cuts at: 1678 2725 3036 4977 1678
Size: 1047 311 1941 2531
Fragments arranged by size:
2531 1941 1047 311
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4471 4503 4545 4577 1373
Size: 32 135 32 2899 32 42 32 2626
Fragments arranged by size:
2899 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2956 3174 3420 669
Size: 988 281 1018 218 246 3079
Fragments arranged by size:
3079 1018 988 281 246 218
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5830
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KasI G'GCGC_C
Cuts at: 2721 2721
Size: 5830
KpnI G_GTAC'C
Cuts at: 3155 3155
Size: 5830
MfeI C'AATT_G
Cuts at: 3429 3429
Size: 5830
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3030 4944 5128 1305
Size: 1725 1914 184 2007
Fragments arranged by size:
2007 1914 1725 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2531 2620 10
Size: 54 1425 343 699 89 3220
Fragments arranged by size:
3220 1425 699 343 89 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2312 2375 3788 4147 4306 518
Size: 648 1146 63 1413 359 159 2042
Fragments arranged by size:
2042 1413 1146 648 359 159 63
MspA1I CmG'CkG
Cuts at: 1606 2293 3246 3923 5071 5316 1606
Size: 687 953 677 1148 245 2120
Fragments arranged by size:
2120 1148 953 687 677 245
NarI GG'CG_CC
Cuts at: 2722 2722
Size: 5830
NcoI C'CATG_G
Cuts at: 513 1065 2937 513
Size: 552 1872 3406
Fragments arranged by size:
3406 1872 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5830
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5830
NotI GC'GGCC_GC
Cuts at: 3277 3277
Size: 5830
NspI r_CATG'y
Cuts at: 1132 1229 2374 2852 3188 4733 1132
Size: 97 1145 478 336 1545 2229
Fragments arranged by size:
2229 1545 1145 478 336 97
PasI CC'CwG_GG
Cuts at: 1348 2074 1348
Size: 726 5104
PciI A'CATG_T
Cuts at: 4729 4729
Size: 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5830
PfoI T'CCnGG_A
Cuts at: 2541 2541
Size: 5830
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 5412 5444 3899
Size: 32 1481 32 4285
Fragments arranged by size:
4285 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2170 3096 2170
Size: 926 4904
PshAI GACnn'nnGTC
Cuts at: 1613 2300 1613
Size: 687 5143
PsiI TTA'TAA
Cuts at: 3400 3400
Size: 5830
PstI C_TGCA'G
Cuts at: 830 2730 3182 830
Size: 1900 452 3478
Fragments arranged by size:
3478 1900 452
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
SalI G'TCGA_C
Cuts at: 1936 3172 1936
Size: 1236 4594
SanDI GG'GwC_CC
Cuts at: 3096 3096
Size: 5830
SbfI CC_TGCA'GG
Cuts at: 3182 3182
Size: 5830
ScaI AGT'ACT
Cuts at: 2672 4078 5790 2672
Size: 1406 1712 2712
Fragments arranged by size:
2712 1712 1406
SfcI C'TryA_G
Cuts at: 826 1045 2146 2726 2761 3178 4313 4994 5185
Size: 219 1101 580 35 417 1135 681 191
Cuts at: 5185 826
Size: 1471
Fragments arranged by size:
1471 1135 1101 681 580 417 219 191
35
SfoI GGC'GCC
Cuts at: 2723 2723
Size: 5830
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 3157 1872
Size: 1285 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3934 4835 5097 820
Size: 197 926 1991 901 262 1553
Fragments arranged by size:
1991 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3621 493
Size: 3128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5830
SphI G_CATG'C
Cuts at: 1132 3188 1132
Size: 2056 3774
SspI AAT'ATT
Cuts at: 5 52 3754 5
Size: 47 3702 2081
Fragments arranged by size:
3702 2081 47
StuI AGG'CCT
Cuts at: 1517 2471 1517
Size: 954 4876
StyI C'CwwG_G
Cuts at: 513 1065 2571 2937 3269 513
Size: 552 1506 366 332 3074
Fragments arranged by size:
3074 1506 552 366 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3857 3874 4027 4212 4657 325
Size: 1255 2277 17 153 185 445 1498
Fragments arranged by size:
2277 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2670 3073 4076
Size: 274 80 33 51 1443 693 403 1003
Cuts at: 4076 5788 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1003 693 403 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5790 5822 5790
Size: 32 5798
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5830
XbaI T'CTAG_A
Cuts at: 3166 3166
Size: 5830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2539 2539
Size: 5830
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5830
XmaI C'CCGG_G
Cuts at: 1870 3155 1870
Size: 1285 4545
XmnI GAAnn'nnTTC
Cuts at: 2552 3959 2552
Size: 1407 4423
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI
SexAI SfiI SrfI SwaI