Restriction map for
pFN21AB9846
(Circular) (Six-base) MAPSORT of: pFN21AB9846.seq Check: 7598 from: 1 to: 5827
pFN21AB9846 5827 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2277 3637 278
Size: 53 83 186 936 741 1360 2468
Fragments arranged by size:
2468 1360 936 741 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3170 1937
Size: 1233 4594
Acc65I G'GTAC_C
Cuts at: 3148 3148
Size: 5827
AclI AA'CG_TT
Cuts at: 1563 2854 3954 4327 1563
Size: 1291 1100 373 3063
Fragments arranged by size:
3063 1291 1100 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2630 2855 3890 5274 5542 1632
Size: 998 225 1035 1384 268 1917
Fragments arranged by size:
1917 1384 1035 998 268 225
AfeI AGC'GCT
Cuts at: 2588 2811 2588
Size: 223 5604
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5630
AflIII A'CryG_T
Cuts at: 1203 4726 5703 1203
Size: 3523 977 1327
Fragments arranged by size:
3523 1327 977
AgeI A'CCGG_T
Cuts at: 4658 4658
Size: 5827
AhdI GACnn_n'nnGTC
Cuts at: 4556 4556
Size: 5827
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5827
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5827
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2664 2696 2664
Size: 32 5795
AlwNI CAG_nnn'CTG
Cuts at: 2026 2079 3570 5142 2026
Size: 53 1491 1572 2711
Fragments arranged by size:
2711 1572 1491 53
ApaI G_GGCC'C
Cuts at: 2311 2311
Size: 5827
ApaLI G'TGCA_C
Cuts at: 2432 3884 5040 2432
Size: 1452 1156 3219
Fragments arranged by size:
3219 1452 1156
ApoI r'AATT_y
Cuts at: 1493 2734 3136 3362 3514 5623 1493
Size: 1241 402 226 152 2109 1697
Fragments arranged by size:
2109 1697 1241 402 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4381 160
Size: 872 3349 1606
Fragments arranged by size:
3349 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
AvaI C'yCGr_G
Cuts at: 1870 1943 2312 2359 3152 1870
Size: 73 369 47 793 4545
Fragments arranged by size:
4545 793 369 73 47
BamHI G'GATC_C
Cuts at: 3157 3524 3157
Size: 367 5460
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3148 4603 618
Size: 325 217 627 1361 1455 1842
Fragments arranged by size:
1842 1455 1361 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2311 3146 1391
Size: 920 835 4072
Fragments arranged by size:
4072 920 835
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2501 928
Size: 971 602 4254
Fragments arranged by size:
4254 971 602
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2012 2046 4018 4052 1903
Size: 34 75 34 1972 34 3678
Fragments arranged by size:
3678 1972 75 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3719 4929 3719
Size: 1210 4617
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5827
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4438 136
Size: 107 122 71 631 605 2766 1525
Fragments arranged by size:
2766 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5822 5822
Size: 5827
BlpI GC'TnA_GC
Cuts at: 3244 3244
Size: 5827
Bme1580I G_kGCm'C
Cuts at: 1790 2311 2436 2913 3888 5044 1790
Size: 521 125 477 975 1156 2573
Fragments arranged by size:
2573 1156 975 521 477 125
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5827
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2854 3558 4516
Size: 416 53 83 399 24 1437 704 958
Cuts at: 4516 442
Size: 1753
Fragments arranged by size:
1753 1437 958 704 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 2318 2459 2537 2715 3011 4487 1239
Size: 959 120 141 78 178 296 1476 2579
Fragments arranged by size:
2579 1476 959 296 178 141 120 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2041 3952 4817 5115 2041
Size: 1911 865 298 2753
Fragments arranged by size:
2753 1911 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2059 2987 3107 4490 882
Size: 617 560 928 120 1383 2219
Fragments arranged by size:
2219 1383 928 617 560 120
BsaAI yAC'GTr
Cuts at: 493 1204 3618 493
Size: 711 2414 2702
Fragments arranged by size:
2702 2414 711
BsaBI GATnn'nnATC
Cuts at: 5821 5821
Size: 5827
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2274 2364 2373 3634
Size: 53 83 186 936 741 90 9 1261
Cuts at: 3634 4016 4651 275
Size: 382 635 1451
Fragments arranged by size:
1451 1261 936 741 635 382 186 90
83 53 9
BsaWI w'CCGG_w
Cuts at: 4260 4658 4932 5079 5490 4260
Size: 398 274 147 411 4597
Fragments arranged by size:
4597 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5602
Fragments arranged by size:
5602 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2396 2933 1186
Size: 1210 537 4080
Fragments arranged by size:
4080 1210 537
BseYI C'CCAG_C
Cuts at: 2770 2806 5030 5526 2770
Size: 36 2224 496 3071
Fragments arranged by size:
3071 2224 496 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2174 2360 1163
Size: 573 438 186 4630
Fragments arranged by size:
4630 573 438 186
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3277 4038 5066 1540
Size: 275 186 1276 761 1028 2301
Fragments arranged by size:
2301 1276 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2436 3888 3973 5044 2436
Size: 1452 85 1071 3219
Fragments arranged by size:
3219 1452 1071 85
BsmI GAATG_Cn'
Cuts at: 3338 3431 3338
Size: 93 5734
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2311 2436 2913 3146 3888 3973 5044
Size: 399 521 125 477 233 742 85 1071
Cuts at: 5044 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 521 477 399 233 125
85
BspEI T'CCGG_A
Cuts at: 5490 5490
Size: 5827
BspHI T'CATG_A
Cuts at: 3716 5446 3716
Size: 1730 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2133 3182 844
Size: 1289 1049 3489
Fragments arranged by size:
3489 1289 1049
BsrBI CCG'CTC
Cuts at: 1394 1823 2182 3714 1394
Size: 429 359 1532 3507
Fragments arranged by size:
3507 1532 429 359
BsrDI GCAATG_nn'
Cuts at: 65 1248 4322 4496 65
Size: 1183 3074 174 1396
Fragments arranged by size:
3074 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2418 4471 4658 1700
Size: 110 608 2053 187 2869
Fragments arranged by size:
2869 2053 608 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5827
BssHII G'CGCG_C
Cuts at: 1925 2117 1925
Size: 192 5635
BssSI C'ACGA_G
Cuts at: 3887 4899 3887
Size: 1012 4815
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2440 794
Size: 1646 4181
BstBI TT'CG_AA
Cuts at: 4631 4631
Size: 5827
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5827
BstYI r'GATC_y
Cuts at: 1972 3157 3524 3909 3926 5378 5464 5476 5822
Size: 1185 367 385 17 1452 86 12 346
Cuts at: 5822 1972
Size: 1977
Fragments arranged by size:
1977 1452 1185 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4336
Fragments arranged by size:
4336 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5088
Fragments arranged by size:
5088 683 56
BtsI GCAGTG_nn'
Cuts at: 2117 3343 4137 4157 2117
Size: 1226 794 20 3787
Fragments arranged by size:
3787 1226 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5827
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5792
DraI TTT'AAA
Cuts at: 3131 3131
Size: 5827
DraIII CAC_nnn'GTG
Cuts at: 2338 2338
Size: 5827
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4834 809
Size: 507 3518 1802
Fragments arranged by size:
3518 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3274 4163 5792
Size: 54 1425 50 275 18 1444 889 1629
Cuts at: 5792 8
Size: 43
Fragments arranged by size:
1629 1444 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3274 1537
Size: 275 1462 4090
Fragments arranged by size:
4090 1462 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2658 3069 3757 1353
Size: 1305 411 688 3423
Fragments arranged by size:
3423 1305 688 411
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2519 4414 4788 4934 387
Size: 1064 138 930 1895 374 146 1280
Fragments arranged by size:
1895 1280 1064 930 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2318 2459 2537 2630 2715 2855
Size: 393 566 120 141 78 93 85 140
Cuts at: 2855 3011 3890 4487 5274 5542 1239
Size: 156 879 597 787 268 1524
Fragments arranged by size:
1524 879 787 597 566 393 268 156
141 140 120 93 85 78
EcoNI CCTnn'n_nnAGG
Cuts at: 2948 2948
Size: 5827
EcoO109I rG'GnC_Cy
Cuts at: 2083 2257 2308 2953 2083
Size: 174 51 645 4957
Fragments arranged by size:
4957 645 174 51
EcoRI G'AATT_C
Cuts at: 2734 3136 3514 2734
Size: 402 378 5047
Fragments arranged by size:
5047 402 378
FspI TGC'GCA
Cuts at: 4333 4333
Size: 5827
HaeII r_GCGC'y
Cuts at: 1678 2146 2590 2630 2813 4974 1678
Size: 468 444 40 183 2161 2531
Fragments arranged by size:
2531 2161 468 444 183 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4468 4500 4542 4574 1373
Size: 32 135 32 2896 32 42 32 2626
Fragments arranged by size:
2896 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2852 3171 3417 669
Size: 988 281 914 319 246 3079
Fragments arranged by size:
3079 988 914 319 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5827
HpaI GTT'AAC
Cuts at: 3417 3417
Size: 5827
KpnI G_GTAC'C
Cuts at: 3152 3152
Size: 5827
MfeI C'AATT_G
Cuts at: 3426 3426
Size: 5827
MluI A'CGCG_T
Cuts at: 5703 5703
Size: 5827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2649 2869 4941 5125 1305
Size: 1344 220 2072 184 2007
Fragments arranged by size:
2072 2007 1344 220 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4005
Fragments arranged by size:
4005 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3785 4144 4303 518
Size: 648 2619 359 159 2042
Fragments arranged by size:
2619 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3243 3920 5068 5313 1606
Size: 1637 677 1148 245 2120
Fragments arranged by size:
2120 1637 1148 677 245
NaeI GCC'GGC
Cuts at: 2420 2420
Size: 5827
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4336
Fragments arranged by size:
4336 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5827
NgoMIV G'CCGG_C
Cuts at: 2418 2418
Size: 5827
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5827
NotI GC'GGCC_GC
Cuts at: 3274 3274
Size: 5827
NspI r_CATG'y
Cuts at: 1132 1229 2440 3185 4730 1132
Size: 97 1211 745 1545 2229
Fragments arranged by size:
2229 1545 1211 745 97
PasI CC'CwG_GG
Cuts at: 1348 2913 1348
Size: 1565 4262
PciI A'CATG_T
Cuts at: 4726 4726
Size: 5827
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5827
PfoI T'CCnGG_A
Cuts at: 2236 2367 2475 2236
Size: 131 108 5588
Fragments arranged by size:
5588 131 108
PmeI GTTT'AAAC
Cuts at: 3131 3131
Size: 5827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 5409 5441 3896
Size: 32 1481 32 4282
Fragments arranged by size:
4282 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2083 2083
Size: 5827
PshAI GACnn'nnGTC
Cuts at: 1613 2847 1613
Size: 1234 4593
PsiI TTA'TAA
Cuts at: 3397 3397
Size: 5827
PspOMI G'GGCC_C
Cuts at: 2307 2307
Size: 5827
PstI C_TGCA'G
Cuts at: 830 2022 2529 3179 830
Size: 1192 507 650 3478
Fragments arranged by size:
3478 1192 650 507
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5827
SalI G'TCGA_C
Cuts at: 1936 3169 1936
Size: 1233 4594
SanDI GG'GwC_CC
Cuts at: 2083 2083
Size: 5827
SapI GCTCTTCn'nnn_
Cuts at: 3069 3069
Size: 5827
SbfI CC_TGCA'GG
Cuts at: 3179 3179
Size: 5827
ScaI AGT'ACT
Cuts at: 4075 5787 4075
Size: 1712 4115
SexAI A'CCwGG_T
Cuts at: 2334 3028 2334
Size: 694 5133
SfcI C'TryA_G
Cuts at: 826 1045 2018 2455 2525 3123 3175 4310 4991
Size: 219 973 437 70 598 52 1135 681
Cuts at: 4991 5182 826
Size: 191 1471
Fragments arranged by size:
1471 1135 973 681 598 437 219 191
70 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
SgrAI Cr'CCGG_yG
Cuts at: 1700 2418 1700
Size: 718 5109
SmaI CCC'GGG
Cuts at: 1872 2361 3154 1872
Size: 489 793 4545
Fragments arranged by size:
4545 793 489
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2056 3931 4832 5094 820
Size: 197 926 113 1875 901 262 1553
Fragments arranged by size:
1875 1553 926 901 262 197 113
SnaBI TAC'GTA
Cuts at: 493 3618 493
Size: 3125 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5827
SphI G_CATG'C
Cuts at: 1132 3185 1132
Size: 2053 3774
SrfI GCCC'GGGC
Cuts at: 2361 2361
Size: 5827
SspI AAT'ATT
Cuts at: 5 52 3751 5
Size: 47 3699 2081
Fragments arranged by size:
3699 2081 47
StuI AGG'CCT
Cuts at: 1517 3114 1517
Size: 1597 4230
StyI C'CwwG_G
Cuts at: 513 1065 2004 3266 513
Size: 552 939 1262 3074
Fragments arranged by size:
3074 1262 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2252 2820 3854 3871 4024 4209 4654
Size: 1255 672 568 1034 17 153 185 445
Cuts at: 4654 325
Size: 1498
Fragments arranged by size:
1498 1255 1034 672 568 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4073 5785 96
Size: 274 80 33 51 1443 2096 1712 138
Fragments arranged by size:
2096 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5787 5819 5787
Size: 32 5795
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5827
XbaI T'CTAG_A
Cuts at: 3163 3163
Size: 5827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2144 2690 2144
Size: 546 5281
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5827
XmaI C'CCGG_G
Cuts at: 1870 2359 3152 1870
Size: 489 793 4545
Fragments arranged by size:
4545 793 489
XmnI GAAnn'nnTTC
Cuts at: 2013 2603 3956 2013
Size: 590 1353 3884
Fragments arranged by size:
3884 1353 590
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2275 3635 276
Size: 53 83 186 936 741 1360 2468
Fragments arranged by size:
2468 1360 936 741 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI PvuII RsrII SacI SacII SfiI
SfoI SwaI