Restriction map for
pFN21AB9843
(Circular) (Six-base) MAPSORT of: pFN21AB9843.seq Check: 9272 from: 1 to: 5803
pFN21AB9843 5803 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3613 278
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3146 1937
Size: 1209 4594
Acc65I G'GTAC_C
Cuts at: 3124 3124
Size: 5803
AclI AA'CG_TT
Cuts at: 1563 3930 4303 1563
Size: 2367 373 3063
Fragments arranged by size:
3063 2367 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2224 3000 3866 5250 5518 1632
Size: 592 776 866 1384 268 1917
Fragments arranged by size:
1917 1384 866 776 592 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5606
AflIII A'CryG_T
Cuts at: 1203 4702 5679 1203
Size: 3499 977 1327
Fragments arranged by size:
3499 1327 977
AgeI A'CCGG_T
Cuts at: 4634 4634
Size: 5803
AhdI GACnn_n'nnGTC
Cuts at: 4532 4532
Size: 5803
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5803
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5803
AlwNI CAG_nnn'CTG
Cuts at: 2387 3546 5118 2387
Size: 1159 1572 3072
Fragments arranged by size:
3072 1572 1159
ApaLI G'TGCA_C
Cuts at: 3860 5016 3860
Size: 1156 4647
ApoI r'AATT_y
Cuts at: 1493 2017 2073 2784 2816 2973 3112 3338 3490
Size: 524 56 711 32 157 139 226 152
Cuts at: 3490 5599 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 711 524 226 157 152 139
56 32
AseI AT'TA_AT
Cuts at: 160 1032 2894 4357 160
Size: 872 1862 1463 1606
Fragments arranged by size:
1862 1606 1463 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
AvaI C'yCGr_G
Cuts at: 1870 1943 3128 1870
Size: 73 1185 4545
Fragments arranged by size:
4545 1185 73
BamHI G'GATC_C
Cuts at: 3133 3500 3133
Size: 367 5436
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2490 3124 4579 618
Size: 325 217 627 703 634 1455 1842
Fragments arranged by size:
1842 1455 703 634 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3122 1391
Size: 1731 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3066 928
Size: 971 1167 3665
Fragments arranged by size:
3665 1167 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3994 4028 1903
Size: 34 2057 34 3678
Fragments arranged by size:
3678 2057 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3695 4905 3695
Size: 1210 4593
BclI T'GATC_A
Cuts at: 1548 2026 1548
Size: 478 5325
BfrBI ATG'CAT
Cuts at: 2520 2520
Size: 5803
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4414 136
Size: 107 122 71 631 605 2742 1525
Fragments arranged by size:
2742 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2850 5798 2850
Size: 2948 2855
BlpI GC'TnA_GC
Cuts at: 3220 3220
Size: 5803
Bme1580I G_kGCm'C
Cuts at: 1790 3864 5020 1790
Size: 2074 1156 2573
Fragments arranged by size:
2573 2074 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5803
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3534 4492 442
Size: 416 53 83 399 24 2117 958 1753
Fragments arranged by size:
2117 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2506 4463 1239
Size: 1267 1957 2579
Fragments arranged by size:
2579 1957 1267
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3928 4793 5091 3928
Size: 865 298 4640
Fragments arranged by size:
4640 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4466 882
Size: 617 2967 2219
Fragments arranged by size:
2967 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2328 3594 493
Size: 711 1124 1266 2702
Fragments arranged by size:
2702 1266 1124 711
BsaBI GATnn'nnATC
Cuts at: 2367 2597 5797 2367
Size: 230 3200 2373
Fragments arranged by size:
3200 2373 230
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3610 3992 4627 275
Size: 53 83 186 936 2077 382 635 1451
Fragments arranged by size:
2077 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4236 4634 4908 5055 5466 4236
Size: 398 274 147 411 4573
Fragments arranged by size:
4573 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5578
Fragments arranged by size:
5578 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2466 2687 1186
Size: 1280 221 4302
Fragments arranged by size:
4302 1280 221
BseYI C'CCAG_C
Cuts at: 5006 5502 5006
Size: 496 5307
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2150 2308 1163
Size: 573 414 158 4658
Fragments arranged by size:
4658 573 414 158
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3253 4014 5042 1540
Size: 275 186 1252 761 1028 2301
Fragments arranged by size:
2301 1252 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2333 3864 3949 5020 2333
Size: 1531 85 1071 3116
Fragments arranged by size:
3116 1531 1071 85
BsmI GAATG_Cn'
Cuts at: 2067 2522 3074 3314 3407 2067
Size: 455 552 240 93 4463
Fragments arranged by size:
4463 552 455 240 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2333 3122 3864 3949 5020 1391
Size: 399 543 789 742 85 1071 2174
Fragments arranged by size:
2174 1071 789 742 543 399 85
BspEI T'CCGG_A
Cuts at: 5466 5466
Size: 5803
BspHI T'CATG_A
Cuts at: 3692 5422 3692
Size: 1730 4073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2327 3158 844
Size: 1483 831 3489
Fragments arranged by size:
3489 1483 831
BsrBI CCG'CTC
Cuts at: 1394 1823 3690 1394
Size: 429 1867 3507
Fragments arranged by size:
3507 1867 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4298 4472 65
Size: 1183 3050 174 1396
Fragments arranged by size:
3050 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4447 4634 1700
Size: 110 2637 187 2869
Fragments arranged by size:
2869 2637 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5803
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5803
BssSI C'ACGA_G
Cuts at: 3863 4875 3863
Size: 1012 4791
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5803
BstBI TT'CG_AA
Cuts at: 4607 4607
Size: 5803
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5803
BstXI CCAn_nnnn'nTGG
Cuts at: 2077 2887 2998 2077
Size: 810 111 4882
Fragments arranged by size:
4882 810 111
BstYI r'GATC_y
Cuts at: 1972 2850 3133 3500 3885 3902 5354 5440 5452
Size: 878 283 367 385 17 1452 86 12
Cuts at: 5452 5798 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 878 385 367 346 283 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4312
Fragments arranged by size:
4312 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3106 487
Size: 683 56 1880 3184
Fragments arranged by size:
3184 1880 683 56
BtsI GCAGTG_nn'
Cuts at: 3319 4113 4133 3319
Size: 794 20 4989
Fragments arranged by size:
4989 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5803
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2571 2606 529
Size: 35 2007 35 3726
Fragments arranged by size:
3726 2007 35 35
DraI TTT'AAA
Cuts at: 3107 3107
Size: 5803
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4810 809
Size: 507 3494 1802
Fragments arranged by size:
3494 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2683 3250 4139
Size: 54 1425 50 275 18 853 567 889
Cuts at: 4139 5768 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 853 567 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3250 1537
Size: 275 1438 4090
Fragments arranged by size:
4090 1438 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3733 1353
Size: 2380 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4390 4764 4910 387
Size: 1064 138 2801 374 146 1280
Fragments arranged by size:
2801 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2224 2506 3000 3866 4463 5250 5518
Size: 393 592 282 494 866 597 787 268
Cuts at: 5518 1239
Size: 1524
Fragments arranged by size:
1524 866 787 597 592 494 393 282
268
EcoRI G'AATT_C
Cuts at: 2816 3112 3490 2816
Size: 296 378 5129
Fragments arranged by size:
5129 378 296
FspI TGC'GCA
Cuts at: 4309 4309
Size: 5803
HaeII r_GCGC'y
Cuts at: 1678 4950 1678
Size: 3272 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4444 4476 4518 4550 1373
Size: 32 135 32 2872 32 42 32 2626
Fragments arranged by size:
2872 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2311 3147 3393 669
Size: 988 281 373 836 246 3079
Fragments arranged by size:
3079 988 836 373 281 246
HindIII A'AGCT_T
Cuts at: 748 3083 748
Size: 2335 3468
HpaI GTT'AAC
Cuts at: 3393 3393
Size: 5803
KpnI G_GTAC'C
Cuts at: 3128 3128
Size: 5803
MfeI C'AATT_G
Cuts at: 3402 3402
Size: 5803
MluI A'CGCG_T
Cuts at: 5679 5679
Size: 5803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4917 5101 1305
Size: 3612 184 2007
Fragments arranged by size:
3612 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2685 10
Size: 54 1425 343 853 3128
Fragments arranged by size:
3128 1425 853 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3761 4120 4279 518
Size: 648 2595 359 159 2042
Fragments arranged by size:
2595 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3219 3896 5044 5289 1606
Size: 1613 677 1148 245 2120
Fragments arranged by size:
2120 1613 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4312
Fragments arranged by size:
4312 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5803
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5803
NotI GC'GGCC_GC
Cuts at: 3250 3250
Size: 5803
NsiI A_TGCA'T
Cuts at: 2522 2522
Size: 5803
NspI r_CATG'y
Cuts at: 1132 1229 2423 3161 4706 1132
Size: 97 1194 738 1545 2229
Fragments arranged by size:
2229 1545 1194 738 97
PasI CC'CwG_GG
Cuts at: 1348 2887 1348
Size: 1539 4264
PciI A'CATG_T
Cuts at: 4702 4702
Size: 5803
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5803
PmeI GTTT'AAAC
Cuts at: 3107 3107
Size: 5803
PmlI CAC'GTG
Cuts at: 2328 2328
Size: 5803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3872 3904 5385 5417 3872
Size: 32 1481 32 4258
Fragments arranged by size:
4258 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5803
PsiI TTA'TAA
Cuts at: 3029 3373 3029
Size: 344 5459
PstI C_TGCA'G
Cuts at: 830 3155 830
Size: 2325 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5803
SalI G'TCGA_C
Cuts at: 1936 3145 1936
Size: 1209 4594
SbfI CC_TGCA'GG
Cuts at: 3155 3155
Size: 5803
ScaI AGT'ACT
Cuts at: 4051 5763 4051
Size: 1712 4091
SfcI C'TryA_G
Cuts at: 826 1045 2352 3151 4286 4967 5158 826
Size: 219 1307 799 1135 681 191 1471
Fragments arranged by size:
1471 1307 1135 799 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5803
SmaI CCC'GGG
Cuts at: 1872 3130 1872
Size: 1258 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3907 4808 5070 820
Size: 197 926 1964 901 262 1553
Fragments arranged by size:
1964 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3594 493
Size: 3101 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5803
SphI G_CATG'C
Cuts at: 1132 2423 3161 1132
Size: 1291 738 3774
Fragments arranged by size:
3774 1291 738
SspI AAT'ATT
Cuts at: 5 52 3727 5
Size: 47 3675 2081
Fragments arranged by size:
3675 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5803
StyI C'CwwG_G
Cuts at: 513 1065 2004 2494 3242 513
Size: 552 939 490 748 3074
Fragments arranged by size:
3074 939 748 552 490
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2891 3830 3847 4000 4185 4630 325
Size: 1255 1311 939 17 153 185 445 1498
Fragments arranged by size:
1498 1311 1255 939 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2740 4049 5761
Size: 274 80 33 51 1443 763 1309 1712
Cuts at: 5761 96
Size: 138
Fragments arranged by size:
1712 1443 1309 763 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2528 2560 5763 5795 2528
Size: 32 3203 32 2536
Fragments arranged by size:
3203 2536 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5803
XbaI T'CTAG_A
Cuts at: 3139 3139
Size: 5803
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5803
XmaI C'CCGG_G
Cuts at: 1870 3128 1870
Size: 1258 4545
XmnI GAAnn'nnTTC
Cuts at: 3074 3932 3074
Size: 858 4945
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3611 276
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI