Restriction map for
pFN21AB9842
(Circular) (Six-base) MAPSORT of: pFN21AB9842.seq Check: 5925 from: 1 to: 5785
pFN21AB9842 5785 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3595 278
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2259 2565 3128 1937
Size: 322 306 563 4594
Fragments arranged by size:
4594 563 322 306
Acc65I G'GTAC_C
Cuts at: 3106 3106
Size: 5785
AclI AA'CG_TT
Cuts at: 1563 3912 4285 1563
Size: 2349 373 3063
Fragments arranged by size:
3063 2349 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2399 3045 3848 5232 5500 1632
Size: 767 646 803 1384 268 1917
Fragments arranged by size:
1917 1384 803 767 646 268
AfeI AGC'GCT
Cuts at: 2480 2480
Size: 5785
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5588
AflIII A'CryG_T
Cuts at: 1203 4684 5661 1203
Size: 3481 977 1327
Fragments arranged by size:
3481 1327 977
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 4514 4514
Size: 5785
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5785
AlwNI CAG_nnn'CTG
Cuts at: 2511 3528 5100 2511
Size: 1017 1572 3196
Fragments arranged by size:
3196 1572 1017
ApaLI G'TGCA_C
Cuts at: 3842 4998 3842
Size: 1156 4629
ApoI r'AATT_y
Cuts at: 1493 2492 3094 3320 3472 5581 1493
Size: 999 602 226 152 2109 1697
Fragments arranged by size:
2109 1697 999 602 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4339 160
Size: 872 3307 1606
Fragments arranged by size:
3307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 3110 1870
Size: 73 1167 4545
Fragments arranged by size:
4545 1167 73
BamHI G'GATC_C
Cuts at: 2640 3115 3482 2640
Size: 475 367 4943
Fragments arranged by size:
4943 475 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3106 4561 618
Size: 325 217 627 1319 1455 1842
Fragments arranged by size:
1842 1455 1319 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2890 3104 1391
Size: 1499 214 4072
Fragments arranged by size:
4072 1499 214
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4814
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2860 2894 3976 4010 1903
Size: 34 923 34 1082 34 3678
Fragments arranged by size:
3678 1082 923 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3677 4887 3677
Size: 1210 4575
BclI T'GATC_A
Cuts at: 1548 2679 3006 1548
Size: 1131 327 4327
Fragments arranged by size:
4327 1131 327
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4396 136
Size: 107 122 71 631 605 2724 1525
Fragments arranged by size:
2724 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2059 5780 2059
Size: 3721 2064
BlpI GC'TnA_GC
Cuts at: 2186 3202 2186
Size: 1016 4769
Bme1580I G_kGCm'C
Cuts at: 1790 3069 3846 5002 1790
Size: 1279 777 1156 2573
Fragments arranged by size:
2573 1279 1156 777
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5785
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2644 3516 4474
Size: 416 53 83 399 24 1227 872 958
Cuts at: 4474 442
Size: 1753
Fragments arranged by size:
1753 1227 958 872 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4445 1239
Size: 3206 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2966 3910 4775 5073 2966
Size: 944 865 298 3678
Fragments arranged by size:
3678 944 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2374 2849 4448 882
Size: 617 875 475 1599 2219
Fragments arranged by size:
2219 1599 875 617 475
BsaAI yAC'GTr
Cuts at: 493 1204 3576 493
Size: 711 2372 2702
Fragments arranged by size:
2702 2372 711
BsaBI GATnn'nnATC
Cuts at: 5779 5779
Size: 5785
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3592 3974 4609 275
Size: 53 83 186 936 2059 382 635 1451
Fragments arranged by size:
2059 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4218 4616 4890 5037 5448 4218
Size: 398 274 147 411 4555
Fragments arranged by size:
4555 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5560
Fragments arranged by size:
5560 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2969 1186
Size: 1783 4002
BseYI C'CCAG_C
Cuts at: 2194 2584 4988 5484 2194
Size: 390 2404 496 2495
Fragments arranged by size:
2495 2404 496 390
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2242 2884 3235 3996 5024 1540
Size: 275 186 241 642 351 761 1028 2301
Fragments arranged by size:
2301 1028 761 642 351 275 241 186
BsiHKAI G_wGCw'C
Cuts at: 3846 3931 5002 3846
Size: 85 1071 4629
Fragments arranged by size:
4629 1071 85
BsiWI C'GTAC_G
Cuts at: 2711 2711
Size: 5785
BsmI GAATG_Cn'
Cuts at: 3296 3389 3296
Size: 93 5692
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2890 3069 3104 3846 3931 5002 1391
Size: 399 1100 179 35 742 85 1071 2174
Fragments arranged by size:
2174 1100 1071 742 399 179 85 35
BspEI T'CCGG_A
Cuts at: 5448 5448
Size: 5785
BspHI T'CATG_A
Cuts at: 2832 3674 5404 2832
Size: 842 1730 3213
Fragments arranged by size:
3213 1730 842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2271 3140 844
Size: 1427 869 3489
Fragments arranged by size:
3489 1427 869
BsrBI CCG'CTC
Cuts at: 1394 1823 2244 3672 1394
Size: 429 421 1428 3507
Fragments arranged by size:
3507 1428 429 421
BsrDI GCAATG_nn'
Cuts at: 65 1248 4280 4454 65
Size: 1183 3032 174 1396
Fragments arranged by size:
3032 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4429 4616 1700
Size: 110 2619 187 2869
Fragments arranged by size:
2869 2619 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 2207 3845 4857 2207
Size: 1638 1012 3135
Fragments arranged by size:
3135 1638 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5785
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5785
BstXI CCAn_nnnn'nTGG
Cuts at: 2657 2657
Size: 5785
BstYI r'GATC_y
Cuts at: 1972 2059 2640 3115 3482 3867 3884 5336 5422
Size: 87 581 475 367 385 17 1452 86
Cuts at: 5422 5434 5780 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 581 475 385 367 346 87
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2971 513
Size: 552 939 967 3327
Fragments arranged by size:
3327 967 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5046
Fragments arranged by size:
5046 683 56
BtsI GCAGTG_nn'
Cuts at: 2672 3301 4095 4115 2672
Size: 629 794 20 4342
Fragments arranged by size:
4342 794 629 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 3089 3089
Size: 5785
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4792 809
Size: 507 3476 1802
Fragments arranged by size:
3476 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2239 2280 2442
Size: 54 1425 50 275 18 409 41 162
Cuts at: 2442 2956 3232 4121 5750 8
Size: 514 276 889 1629 43
Fragments arranged by size:
1629 1425 889 514 409 276 275 162
54 50 43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 2239 3232 1537
Size: 275 427 993 4090
Fragments arranged by size:
4090 993 427 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3061 3715 1353
Size: 1708 654 3423
Fragments arranged by size:
3423 1708 654
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2641 4372 4746 4892 387
Size: 1064 138 1052 1731 374 146 1280
Fragments arranged by size:
1731 1280 1064 1052 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2399 3045 3848 4445 5232 5500 1239
Size: 393 767 646 803 597 787 268 1524
Fragments arranged by size:
1524 803 787 767 646 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 2360
Size: 5785
EcoO109I rG'GnC_Cy
Cuts at: 2838 2838
Size: 5785
EcoRI G'AATT_C
Cuts at: 3094 3472 3094
Size: 378 5407
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2500 2500
Size: 5785
FspI TGC'GCA
Cuts at: 4291 4291
Size: 5785
HaeII r_GCGC'y
Cuts at: 1678 2482 2869 4932 1678
Size: 804 387 2063 2531
Fragments arranged by size:
2531 2063 804 387
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4426 4458 4500 4532 1373
Size: 32 135 32 2854 32 42 32 2626
Fragments arranged by size:
2854 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2389 3129 3375 669
Size: 988 281 451 740 246 3079
Fragments arranged by size:
3079 988 740 451 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5785
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KpnI G_GTAC'C
Cuts at: 3110 3110
Size: 5785
MfeI C'AATT_G
Cuts at: 3384 3384
Size: 5785
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4899 5083 1305
Size: 3594 184 2007
Fragments arranged by size:
3594 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2444 2958 10
Size: 54 1425 343 612 514 2837
Fragments arranged by size:
2837 1425 612 514 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2356 2426 2831 3743 4102 4261 518
Size: 648 1190 70 405 912 359 159 2042
Fragments arranged by size:
2042 1190 912 648 405 359 159 70
MspA1I CmG'CkG
Cuts at: 1606 2925 3201 3878 5026 5271 1606
Size: 1319 276 677 1148 245 2120
Fragments arranged by size:
2120 1319 1148 677 276 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2971 513
Size: 552 939 967 3327
Fragments arranged by size:
3327 967 939 552
NdeI CA'TA_TG
Cuts at: 387 2498 387
Size: 2111 3674
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5785
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NspI r_CATG'y
Cuts at: 1132 1229 3143 4688 1132
Size: 97 1914 1545 2229
Fragments arranged by size:
2229 1914 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5785
PciI A'CATG_T
Cuts at: 4684 4684
Size: 5785
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5785
PfoI T'CCnGG_A
Cuts at: 2850 2850
Size: 5785
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 5367 5399 3854
Size: 32 1481 32 4240
Fragments arranged by size:
4240 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2838 2838
Size: 5785
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5785
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PstI C_TGCA'G
Cuts at: 830 3137 830
Size: 2307 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5785
PvuII CAG'CTG
Cuts at: 2925 2925
Size: 5785
SalI G'TCGA_C
Cuts at: 1936 3127 1936
Size: 1191 4594
SapI GCTCTTCn'nnn_
Cuts at: 3061 3061
Size: 5785
SbfI CC_TGCA'GG
Cuts at: 3137 3137
Size: 5785
ScaI AGT'ACT
Cuts at: 4033 5745 4033
Size: 1712 4073
SexAI A'CCwGG_T
Cuts at: 2808 2808
Size: 5785
SfcI C'TryA_G
Cuts at: 826 1045 2395 3133 4268 4949 5140 826
Size: 219 1350 738 1135 681 191 1471
Fragments arranged by size:
1471 1350 1135 738 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 3112 1872
Size: 1240 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2981 3889 4790 5052 820
Size: 197 926 1038 908 901 262 1553
Fragments arranged by size:
1553 1038 926 908 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5785
SphI G_CATG'C
Cuts at: 1132 3143 1132
Size: 2011 3774
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 3040 1517
Size: 1523 4262
StyI C'CwwG_G
Cuts at: 513 1065 2004 2277 2325 2971 3224 513
Size: 552 939 273 48 646 253 3074
Fragments arranged by size:
3074 939 646 552 273 253 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3812 3829 3982 4167 4612 325
Size: 1255 2232 17 153 185 445 1498
Fragments arranged by size:
2232 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4031 5743 96
Size: 274 80 33 51 1443 2054 1712 138
Fragments arranged by size:
2054 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5745 5777 5745
Size: 32 5753
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5785
XbaI T'CTAG_A
Cuts at: 3121 3121
Size: 5785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2905 2905
Size: 5785
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5785
XmaI C'CCGG_G
Cuts at: 1870 3110 1870
Size: 1240 4545
XmnI GAAnn'nnTTC
Cuts at: 3914 3914
Size: 5785
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3593 276
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI