Restriction map for
pFN21AB9840
(Circular) (Six-base) MAPSORT of: pFN21AB9840.seq Check: 1506 from: 1 to: 5773
pFN21AB9840 5773 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3583 278
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3116 1937
Size: 1179 4594
Acc65I G'GTAC_C
Cuts at: 3094 3094
Size: 5773
AclI AA'CG_TT
Cuts at: 1563 3900 4273 1563
Size: 2337 373 3063
Fragments arranged by size:
3063 2337 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3836 5220 5488 1632
Size: 2204 1384 268 1917
Fragments arranged by size:
2204 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5576
AflIII A'CryG_T
Cuts at: 1203 4672 5649 1203
Size: 3469 977 1327
Fragments arranged by size:
3469 1327 977
AgeI A'CCGG_T
Cuts at: 4604 4604
Size: 5773
AhdI GACnn_n'nnGTC
Cuts at: 4502 4502
Size: 5773
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5773
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5773
AlwNI CAG_nnn'CTG
Cuts at: 3516 5088 3516
Size: 1572 4201
ApaI G_GGCC'C
Cuts at: 2938 2938
Size: 5773
ApaLI G'TGCA_C
Cuts at: 3830 4986 3830
Size: 1156 4617
ApoI r'AATT_y
Cuts at: 1493 3082 3308 3460 5569 1493
Size: 1589 226 152 2109 1697
Fragments arranged by size:
2109 1697 1589 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2910 4327 160
Size: 872 1878 1417 1606
Fragments arranged by size:
1878 1606 1417 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
AvaI C'yCGr_G
Cuts at: 1870 1943 3098 1870
Size: 73 1155 4545
Fragments arranged by size:
4545 1155 73
BamHI G'GATC_C
Cuts at: 2118 3103 3470 2118
Size: 985 367 4421
Fragments arranged by size:
4421 985 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2314 2892 2900 3094 4549
Size: 325 217 627 527 578 8 194 1455
Cuts at: 4549 618
Size: 1842
Fragments arranged by size:
1842 1455 627 578 527 325 217 194
8
BanII G_rGCy'C
Cuts at: 1391 2938 3092 1391
Size: 1547 154 4072
Fragments arranged by size:
4072 1547 154
BbeI G_GCGC'C
Cuts at: 2896 2904 2896
Size: 8 5765
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4802
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3964 3998 1903
Size: 34 2027 34 3678
Fragments arranged by size:
3678 2027 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3665 4875 3665
Size: 1210 4563
BclI T'GATC_A
Cuts at: 1548 2226 1548
Size: 678 5095
BfrBI ATG'CAT
Cuts at: 2831 2831
Size: 5773
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4384 136
Size: 107 122 71 631 605 2712 1525
Fragments arranged by size:
2712 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5768 5768
Size: 5773
BlpI GC'TnA_GC
Cuts at: 3190 3190
Size: 5773
Bme1580I G_kGCm'C
Cuts at: 1790 2938 3834 4990 1790
Size: 1148 896 1156 2573
Fragments arranged by size:
2573 1156 1148 896
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5773
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2320 2512 2570
Size: 416 53 83 399 24 903 192 58
Cuts at: 2570 2951 3016 3504 4462 442
Size: 381 65 488 958 1753
Fragments arranged by size:
1753 958 903 488 416 399 381 192
83 65 58 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5773
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 5773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2525 2666 2773 2781 4433 1239
Size: 1022 264 141 107 8 1652 2579
Fragments arranged by size:
2579 1652 1022 264 141 107 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3898 4763 5061 3898
Size: 865 298 4610
Fragments arranged by size:
4610 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4436 882
Size: 617 2937 2219
Fragments arranged by size:
2937 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3564 493
Size: 711 2360 2702
Fragments arranged by size:
2702 2360 711
BsaBI GATnn'nnATC
Cuts at: 2147 5767 2147
Size: 3620 2153
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2893 2901 3580 3962
Size: 53 83 186 936 1360 8 679 382
Cuts at: 3962 4597 275
Size: 635 1451
Fragments arranged by size:
1451 1360 936 679 635 382 186 83
53 8
BsaWI w'CCGG_w
Cuts at: 2167 2768 4206 4604 4878 5025 5436 2167
Size: 601 1438 398 274 147 411 2504
Fragments arranged by size:
2504 1438 601 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2680 2710 1178
Size: 30 165 30 1277 30 4241
Fragments arranged by size:
4241 1277 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 2959 1186
Size: 955 818 4000
Fragments arranged by size:
4000 955 818
BseYI C'CCAG_C
Cuts at: 4976 5472 4976
Size: 496 5277
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2626 3088 1163
Size: 573 890 462 3848
Fragments arranged by size:
3848 890 573 462
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3223 3984 5012 1540
Size: 275 186 1222 761 1028 2301
Fragments arranged by size:
2301 1222 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3834 3919 4990 3834
Size: 85 1071 4617
Fragments arranged by size:
4617 1071 85
BsmI GAATG_Cn'
Cuts at: 3284 3377 3284
Size: 93 5680
BsmBI CGTCTCn'nnnn_
Cuts at: 2501 2501
Size: 5773
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2938 3092 3834 3919 4990 1391
Size: 399 1148 154 742 85 1071 2174
Fragments arranged by size:
2174 1148 1071 742 399 154 85
BspEI T'CCGG_A
Cuts at: 2167 5436 2167
Size: 3269 2504
BspHI T'CATG_A
Cuts at: 2538 3662 5392 2538
Size: 1124 1730 2919
Fragments arranged by size:
2919 1730 1124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2383 2971 3128 844
Size: 1539 588 157 3489
Fragments arranged by size:
3489 1539 588 157
BsrBI CCG'CTC
Cuts at: 1394 1823 2434 2701 3660 1394
Size: 429 611 267 959 3507
Fragments arranged by size:
3507 959 611 429 267
BsrDI GCAATG_nn'
Cuts at: 65 1248 4268 4442 65
Size: 1183 3020 174 1396
Fragments arranged by size:
3020 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2012 2189 2317 2563 4417 4604 1700
Size: 110 202 177 128 246 1854 187 2869
Fragments arranged by size:
2869 1854 246 202 187 177 128 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5773
BssHII G'CGCG_C
Cuts at: 1925 2136 1925
Size: 211 5562
BssSI C'ACGA_G
Cuts at: 3833 4845 3833
Size: 1012 4761
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3066 794
Size: 2272 3501
BstBI TT'CG_AA
Cuts at: 4577 4577
Size: 5773
BstEII G'GTnAC_C
Cuts at: 1184 2296 1184
Size: 1112 4661
BstXI CCAn_nnnn'nTGG
Cuts at: 2959 2959
Size: 5773
BstYI r'GATC_y
Cuts at: 1972 2118 2370 2916 3103 3470 3855 3872 5324
Size: 146 252 546 187 367 385 17 1452
Cuts at: 5324 5410 5422 5768 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 546 385 367 346 252 187
146 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2303 2303
Size: 5773
BtgI C'CryG_G
Cuts at: 513 1065 2004 2277 513
Size: 552 939 273 4009
Fragments arranged by size:
4009 939 552 273
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2431 487
Size: 683 56 1205 3829
Fragments arranged by size:
3829 1205 683 56
BtsI GCAGTG_nn'
Cuts at: 3289 4083 4103 3289
Size: 794 20 4959
Fragments arranged by size:
4959 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5773
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5738
DraI TTT'AAA
Cuts at: 3077 3077
Size: 5773
DraIII CAC_nnn'GTG
Cuts at: 2328 2475 2328
Size: 147 5626
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4780 809
Size: 507 3464 1802
Fragments arranged by size:
3464 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2280 2319 2565
Size: 54 1425 50 275 18 450 39 246
Cuts at: 2565 2593 2709 3220 4109 5738 8
Size: 28 116 511 889 1629 43
Fragments arranged by size:
1629 1425 889 511 450 275 246 116
54 50 43 39 28 18
EagI C'GGCC_G
Cuts at: 1537 1812 3220 1537
Size: 275 1408 4090
Fragments arranged by size:
4090 1408 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3703 1353
Size: 2350 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2909 4360 4734 4880 387
Size: 1064 138 1320 1451 374 146 1280
Fragments arranged by size:
1451 1320 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2525 2666 2773 2781 3836 4433
Size: 393 629 264 141 107 8 1055 597
Cuts at: 4433 5220 5488 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1055 787 629 597 393 268 264
141 107 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2974 2974
Size: 5773
EcoO109I rG'GnC_Cy
Cuts at: 2289 2934 2289
Size: 645 5128
EcoRI G'AATT_C
Cuts at: 3082 3460 3082
Size: 378 5395
EcoRV GAT'ATC
Cuts at: 2251 2251
Size: 5773
FspI TGC'GCA
Cuts at: 4279 4279
Size: 5773
HaeII r_GCGC'y
Cuts at: 1678 2896 2904 4920 1678
Size: 1218 8 2016 2531
Fragments arranged by size:
2531 2016 1218 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2654 2686 2777 2809 4414
Size: 32 135 32 1082 32 91 32 1605
Cuts at: 4414 4446 4488 4520 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1605 1082 135 91 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3117 3363 669
Size: 988 281 1179 246 3079
Fragments arranged by size:
3079 1179 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5773
HpaI GTT'AAC
Cuts at: 3363 3363
Size: 5773
KasI G'GCGC_C
Cuts at: 2892 2900 2892
Size: 8 5765
KpnI G_GTAC'C
Cuts at: 3098 3098
Size: 5773
MfeI C'AATT_G
Cuts at: 3372 3372
Size: 5773
MluI A'CGCG_T
Cuts at: 5649 5649
Size: 5773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4887 5071 1305
Size: 3582 184 2007
Fragments arranged by size:
3582 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2711 10
Size: 54 1425 343 879 3072
Fragments arranged by size:
3072 1425 879 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3731 4090 4249 518
Size: 648 2565 359 159 2042
Fragments arranged by size:
2565 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2279 3189 3866 5014 5259 1606
Size: 673 910 677 1148 245 2120
Fragments arranged by size:
2120 1148 910 677 673 245
NaeI GCC'GGC
Cuts at: 2319 2565 2319
Size: 246 5527
NarI GG'CG_CC
Cuts at: 2893 2901 2893
Size: 8 5765
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4282
Fragments arranged by size:
4282 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5773
NgoMIV G'CCGG_C
Cuts at: 2317 2563 2317
Size: 246 5527
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5773
NotI GC'GGCC_GC
Cuts at: 3220 3220
Size: 5773
NsiI A_TGCA'T
Cuts at: 2833 2833
Size: 5773
NspI r_CATG'y
Cuts at: 1132 1229 3131 4676 1132
Size: 97 1902 1545 2229
Fragments arranged by size:
2229 1902 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5773
PciI A'CATG_T
Cuts at: 4672 4672
Size: 5773
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2644 1108
Size: 1536 4237
PmeI GTTT'AAAC
Cuts at: 3077 3077
Size: 5773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 5355 5387 3842
Size: 32 1481 32 4228
Fragments arranged by size:
4228 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2737 1613
Size: 1124 4649
PsiI TTA'TAA
Cuts at: 3343 3343
Size: 5773
PspOMI G'GGCC_C
Cuts at: 2934 2934
Size: 5773
PstI C_TGCA'G
Cuts at: 830 3125 830
Size: 2295 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5773
SacII CC_GC'GG
Cuts at: 2280 2280
Size: 5773
SalI G'TCGA_C
Cuts at: 1936 3115 1936
Size: 1179 4594
SbfI CC_TGCA'GG
Cuts at: 3125 3125
Size: 5773
ScaI AGT'ACT
Cuts at: 4021 5733 4021
Size: 1712 4061
SexAI A'CCwGG_T
Cuts at: 2721 2721
Size: 5773
SfcI C'TryA_G
Cuts at: 826 1045 3121 4256 4937 5128 826
Size: 219 2076 1135 681 191 1471
Fragments arranged by size:
2076 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2894 2902 2894
Size: 8 5765
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5773
SmaI CCC'GGG
Cuts at: 1872 3100 1872
Size: 1228 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3877 4778 5040 820
Size: 197 926 1934 901 262 1553
Fragments arranged by size:
1934 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3564 493
Size: 3071 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5773
SphI G_CATG'C
Cuts at: 1132 3131 1132
Size: 1999 3774
SspI AAT'ATT
Cuts at: 5 52 3697 5
Size: 47 3645 2081
Fragments arranged by size:
3645 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5773
StyI C'CwwG_G
Cuts at: 513 1065 2004 2896 3212 513
Size: 552 939 892 316 3074
Fragments arranged by size:
3074 939 892 552 316
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2339 2651 2861 3800 3817 3970 4155
Size: 1255 759 312 210 939 17 153 185
Cuts at: 4155 4600 325
Size: 445 1498
Fragments arranged by size:
1498 1255 939 759 445 312 210 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4019 5731 96
Size: 274 80 33 51 1443 2042 1712 138
Fragments arranged by size:
2042 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2022 2054 2680 2712 5733 5765 2022
Size: 32 626 32 3021 32 2030
Fragments arranged by size:
3021 2030 626 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5773
XbaI T'CTAG_A
Cuts at: 2659 3109 2659
Size: 450 5323
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2641 3013 2641
Size: 372 5401
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5773
XmaI C'CCGG_G
Cuts at: 1870 3098 1870
Size: 1228 4545
XmnI GAAnn'nnTTC
Cuts at: 3902 3902
Size: 5773
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3581 276
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI Bpu10I
BsiWI BstZ17I EcoICRI FalI FseI FspAI NruI PacI
PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SanDI SapI SfiI SrfI SwaI