Restriction map for
pFN21AB9838
(Circular) (Six-base) MAPSORT of: pFN21AB9838.seq Check: 9616 from: 1 to: 5761
pFN21AB9838 5761 bp
With 182 enzymes: SgfI *
March 28, 2012 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3571 278
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3104 1937
Size: 1167 4594
Acc65I G'GTAC_C
Cuts at: 3082 3082
Size: 5761
AclI AA'CG_TT
Cuts at: 1563 3888 4261 1563
Size: 2325 373 3063
Fragments arranged by size:
3063 2325 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2725 2909 3824 5208 5476 1632
Size: 1093 184 915 1384 268 1917
Fragments arranged by size:
1917 1384 1093 915 268 184
AfeI AGC'GCT
Cuts at: 2123 2678 2123
Size: 555 5206
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5564
AflIII A'CryG_T
Cuts at: 1203 4660 5637 1203
Size: 3457 977 1327
Fragments arranged by size:
3457 1327 977
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 4490 4490
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1166 2643 1166
Size: 1477 4284
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5761
AlwNI CAG_nnn'CTG
Cuts at: 2208 3504 5076 2208
Size: 1296 1572 2893
Fragments arranged by size:
2893 1572 1296
ApaI G_GGCC'C
Cuts at: 2306 2306
Size: 5761
ApaLI G'TGCA_C
Cuts at: 3818 4974 3818
Size: 1156 4605
ApoI r'AATT_y
Cuts at: 1493 3070 3296 3448 5557 1493
Size: 1577 226 152 2109 1697
Fragments arranged by size:
2109 1697 1577 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 2111 2221 2285 2326 2387 2408 2509
Size: 73 168 110 64 41 61 21 101
Cuts at: 2509 2969 3086 1870
Size: 460 117 4545
Fragments arranged by size:
4545 460 168 117 110 101 73 64
61 41 21
BamHI G'GATC_C
Cuts at: 3091 3458 3091
Size: 367 5394
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2027 2225 2279 2309 2321
Size: 325 217 627 240 198 54 30 12
Cuts at: 2321 2546 2904 3082 4537 618
Size: 225 358 178 1455 1842
Fragments arranged by size:
1842 1455 627 358 325 240 225 217
198 178 54 30 12
BanII G_rGCy'C
Cuts at: 1391 2306 3080 1391
Size: 915 774 4072
Fragments arranged by size:
4072 915 774
BbeI G_GCGC'C
Cuts at: 2031 2283 2313 2550 2908 2031
Size: 252 30 237 358 4884
Fragments arranged by size:
4884 358 252 237 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4790
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3952 3986 1903
Size: 34 2015 34 3678
Fragments arranged by size:
3678 2015 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3653 4863 3653
Size: 1210 4551
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5761
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2448 2495 2964
Size: 107 122 71 631 605 776 47 469
Cuts at: 2964 4372 136
Size: 1408 1525
Fragments arranged by size:
1525 1408 776 631 605 469 122 107
71 47
BglII A'GATC_T
Cuts at: 5756 5756
Size: 5761
BlpI GC'TnA_GC
Cuts at: 2168 3178 2168
Size: 1010 4751
Bme1580I G_kGCm'C
Cuts at: 1790 2193 2228 2306 2324 3044 3822 4978 1790
Size: 403 35 78 18 720 778 1156 2573
Fragments arranged by size:
2573 1156 778 720 403 78 35 18
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5761
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3492 4450 442
Size: 416 53 83 399 24 2075 958 1753
Fragments arranged by size:
2075 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5761
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2363 2690 2852 2867 2915 4421 1239
Size: 1124 327 162 15 48 1506 2579
Fragments arranged by size:
2579 1506 1124 327 162 48 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2750 2771 3886 4751 5049 2750
Size: 21 1115 865 298 3462
Fragments arranged by size:
3462 1115 865 298 21
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4424 882
Size: 617 2925 2219
Fragments arranged by size:
2925 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2662 3552 493
Size: 711 1458 890 2702
Fragments arranged by size:
2702 1458 890 711
BsaBI GATnn'nnATC
Cuts at: 5755 5755
Size: 5761
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2028 2162 2280 2310
Size: 53 83 186 936 495 134 118 30
Cuts at: 2310 2405 2547 2905 3568 3950 4585 275
Size: 95 142 358 663 382 635 1451
Fragments arranged by size:
1451 936 663 635 495 382 358 186
142 134 118 95 83 53 30
BsaWI w'CCGG_w
Cuts at: 4194 4592 4866 5013 5424 4194
Size: 398 274 147 411 4531
Fragments arranged by size:
4531 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2336 2366 1178
Size: 30 165 30 933 30 4573
Fragments arranged by size:
4573 933 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2173 2185 2687 2765 1186
Size: 987 12 502 78 4182
Fragments arranged by size:
4182 987 502 78 12
BseYI C'CCAG_C
Cuts at: 2039 2201 2216 4964 5460 2039
Size: 162 15 2748 496 2340
Fragments arranged by size:
2748 2340 496 162 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2849 1163
Size: 573 1113 4075
Fragments arranged by size:
4075 1113 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2481 3048 3211 3972 5000 1540
Size: 275 186 480 567 163 761 1028 2301
Fragments arranged by size:
2301 1028 761 567 480 275 186 163
BsiHKAI G_wGCw'C
Cuts at: 3822 3907 4978 3822
Size: 85 1071 4605
Fragments arranged by size:
4605 1071 85
BsmI GAATG_Cn'
Cuts at: 3272 3365 3272
Size: 93 5668
BsmBI CGTCTCn'nnnn_
Cuts at: 2271 2271
Size: 5761
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2193 2228 2306 2324 3044 3080 3822
Size: 399 403 35 78 18 720 36 742
Cuts at: 3822 3907 4978 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 720 403 399 85 78
36 35 18
BspEI T'CCGG_A
Cuts at: 5424 5424
Size: 5761
BspHI T'CATG_A
Cuts at: 3650 5380 3650
Size: 1730 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3116 844
Size: 2272 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2167 2298 2364 2534 2920 3648 1394
Size: 429 344 131 66 170 386 728 3507
Fragments arranged by size:
3507 728 429 386 344 170 131 66
BsrDI GCAATG_nn'
Cuts at: 65 1248 4256 4430 65
Size: 1183 3008 174 1396
Fragments arranged by size:
3008 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2228 2335 2549 2724 2987 3020 4405
Size: 110 418 107 214 175 263 33 1385
Cuts at: 4405 4592 1700
Size: 187 2869
Fragments arranged by size:
2869 1385 418 263 214 187 175 110
107 33
BsrGI T'GTAC_A
Cuts at: 96 2928 96
Size: 2832 2929
BssHII G'CGCG_C
Cuts at: 1925 2211 2451 2463 2565 1925
Size: 286 240 12 102 5121
Fragments arranged by size:
5121 286 240 102 12
BssSI C'ACGA_G
Cuts at: 3821 4833 3821
Size: 1012 4749
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2178 794
Size: 1384 4377
BstBI TT'CG_AA
Cuts at: 4565 4565
Size: 5761
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5761
BstXI CCAn_nnnn'nTGG
Cuts at: 2624 2624
Size: 5761
BstYI r'GATC_y
Cuts at: 1972 2347 3091 3458 3843 3860 5312 5398 5410
Size: 375 744 367 385 17 1452 86 12
Cuts at: 5410 5756 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 744 385 375 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2010 2446 2458 2503 2560 2623
Size: 552 939 6 436 12 45 57 63
Cuts at: 2623 2734 2794 2996 513
Size: 111 60 202 3278
Fragments arranged by size:
3278 939 552 436 202 111 63 60
57 45 12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2036 487
Size: 683 56 810 4212
Fragments arranged by size:
4212 810 683 56
BtsI GCAGTG_nn'
Cuts at: 3277 4071 4091 3277
Size: 794 20 4947
Fragments arranged by size:
4947 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5726
DraI TTT'AAA
Cuts at: 3065 3065
Size: 5761
DraIII CAC_nnn'GTG
Cuts at: 2662 2662
Size: 5761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4768 809
Size: 507 3452 1802
Fragments arranged by size:
3452 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2052 2440
Size: 54 1425 50 275 18 177 45 388
Cuts at: 2440 2478 2551 2910 3045 3208 4097 5726 8
Size: 38 73 359 135 163 889 1629 43
Fragments arranged by size:
1629 1425 889 388 359 275 177 163
135 73 54 50 45 43 38 18
EagI C'GGCC_G
Cuts at: 1537 1812 2478 3045 3208 1537
Size: 275 666 567 163 4090
Fragments arranged by size:
4090 666 567 275 163
EarI CTCTTCn'nnn_
Cuts at: 1353 3691 1353
Size: 2338 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2544 4348 4722 4868 387
Size: 1064 138 955 1804 374 146 1280
Fragments arranged by size:
1804 1280 1064 955 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2363 2690 2725 2852 2867 2909 2915
Size: 393 731 327 35 127 15 42 6
Cuts at: 2915 3824 4421 5208 5476 1239
Size: 909 597 787 268 1524
Fragments arranged by size:
1524 909 787 731 597 393 327 268
127 42 35 15 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2930 2930
Size: 5761
EcoO109I rG'GnC_Cy
Cuts at: 2302 2303 2923 2302
Size: 1 620 5140
Fragments arranged by size:
5140 620 1
EcoRI G'AATT_C
Cuts at: 3070 3448 3070
Size: 378 5383
FspI TGC'GCA
Cuts at: 4267 4267
Size: 5761
HaeII r_GCGC'y
Cuts at: 1678 2031 2125 2283 2313 2342 2396 2429 2550
Size: 353 94 158 30 29 54 33 121
Cuts at: 2550 2680 2908 4908 1678
Size: 130 228 2000 2531
Fragments arranged by size:
2531 2000 353 228 158 130 121 94
54 33 30 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4402 4434 4476 4508 1373
Size: 32 135 32 2830 32 42 32 2626
Fragments arranged by size:
2830 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2068 3105 3351 669
Size: 988 281 130 1037 246 3079
Fragments arranged by size:
3079 1037 988 281 246 130
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5761
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KasI G'GCGC_C
Cuts at: 2027 2279 2309 2546 2904 2027
Size: 252 30 237 358 4884
Fragments arranged by size:
4884 358 252 237 30
KpnI G_GTAC'C
Cuts at: 3086 3086
Size: 5761
MfeI C'AATT_G
Cuts at: 3360 3360
Size: 5761
MluI A'CGCG_T
Cuts at: 5637 5637
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2709 4875 5059 1305
Size: 1404 2166 184 2007
Fragments arranged by size:
2166 2007 1404 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2054 2912 10
Size: 54 1425 343 222 858 2859
Fragments arranged by size:
2859 1425 858 343 222 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2622 2643 2708 3719 4078 4237 518
Size: 648 1456 21 65 1011 359 159 2042
Fragments arranged by size:
2042 1456 1011 648 359 159 65 21
MspA1I CmG'CkG
Cuts at: 1606 2012 2050 2439 2505 2695 2736 2796 2836
Size: 406 38 389 66 190 41 60 40
Cuts at: 2836 2887 2998 3177 3854 5002 5247 1606
Size: 51 111 179 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 406 389 245 190 179
111 66 60 51 41 40 38
NaeI GCC'GGC
Cuts at: 2337 2551 2337
Size: 214 5547
NarI GG'CG_CC
Cuts at: 2028 2280 2310 2547 2905 2028
Size: 252 30 237 358 4884
Fragments arranged by size:
4884 358 252 237 30
NcoI C'CATG_G
Cuts at: 513 1065 2004 2623 513
Size: 552 939 619 3651
Fragments arranged by size:
3651 939 619 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NgoMIV G'CCGG_C
Cuts at: 2335 2549 2335
Size: 214 5547
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5761
NotI GC'GGCC_GC
Cuts at: 2478 3208 2478
Size: 730 5031
NspI r_CATG'y
Cuts at: 1132 1229 3119 4664 1132
Size: 97 1890 1545 2229
Fragments arranged by size:
2229 1890 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5761
PciI A'CATG_T
Cuts at: 4660 4660
Size: 5761
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5761
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PmlI CAC'GTG
Cuts at: 2662 2662
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 5343 5375 3830
Size: 32 1481 32 4216
Fragments arranged by size:
4216 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2923 2923
Size: 5761
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 3331 3331
Size: 5761
PspOMI G'GGCC_C
Cuts at: 2302 2302
Size: 5761
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2783 2815 2783
Size: 32 5729
PstI C_TGCA'G
Cuts at: 830 3113 830
Size: 2283 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
PvuII CAG'CTG
Cuts at: 2695 2887 2695
Size: 192 5569
SacII CC_GC'GG
Cuts at: 2013 2506 2737 2797 2999 2013
Size: 493 231 60 202 4775
Fragments arranged by size:
4775 493 231 202 60
SalI G'TCGA_C
Cuts at: 1936 3103 1936
Size: 1167 4594
SbfI CC_TGCA'GG
Cuts at: 3113 3113
Size: 5761
ScaI AGT'ACT
Cuts at: 4009 5721 4009
Size: 1712 4049
SfcI C'TryA_G
Cuts at: 826 1045 3109 4244 4925 5116 826
Size: 219 2064 1135 681 191 1471
Fragments arranged by size:
2064 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2964 2964
Size: 5761
SfoI GGC'GCC
Cuts at: 2029 2281 2311 2548 2906 2029
Size: 252 30 237 358 4884
Fragments arranged by size:
4884 358 252 237 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 2228 1700
Size: 528 5233
SmaI CCC'GGG
Cuts at: 1872 2223 2287 2511 2971 3088 1872
Size: 351 64 224 460 117 4545
Fragments arranged by size:
4545 460 351 224 117 64
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2765 2786 3865 4766 5028 820
Size: 197 926 822 21 1079 901 262 1553
Fragments arranged by size:
1553 1079 926 901 822 262 197 21
SnaBI TAC'GTA
Cuts at: 493 3552 493
Size: 3059 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5761
SphI G_CATG'C
Cuts at: 1132 3119 1132
Size: 1987 3774
SrfI GCCC'GGGC
Cuts at: 2223 2511 2223
Size: 288 5473
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5761
StyI C'CwwG_G
Cuts at: 513 1065 2004 2623 3036 3200 513
Size: 552 939 619 413 164 3074
Fragments arranged by size:
3074 939 619 552 413 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3788 3805 3958 4143 4588 325
Size: 1255 2208 17 153 185 445 1498
Fragments arranged by size:
2208 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2352 2928 4007
Size: 274 80 33 51 1443 375 576 1079
Cuts at: 4007 5719 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1079 576 375 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5721 5753 5721
Size: 32 5729
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5761
XbaI T'CTAG_A
Cuts at: 3097 3097
Size: 5761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2047 2621 2047
Size: 574 5187
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 2221 2285 2509 2969 3086 1870
Size: 351 64 224 460 117 4545
Fragments arranged by size:
4545 460 351 224 117 64
XmnI GAAnn'nnTTC
Cuts at: 3890 3890
Size: 5761
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3569 276
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacII SalI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PspXI RsrII SacI SanDI
SapI SexAI SwaI