Restriction map for
pFN21AB9837
(Circular) (Six-base) MAPSORT of: pFN21AB9837.seq Check: 4783 from: 1 to: 5761
pFN21AB9837 5761 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2543 2833 2543
Size: 290 5471
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3571 278
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3104 1937
Size: 1167 4594
Acc65I G'GTAC_C
Cuts at: 2382 3082 2382
Size: 700 5061
AclI AA'CG_TT
Cuts at: 1563 3888 4261 1563
Size: 2325 373 3063
Fragments arranged by size:
3063 2325 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2207 3824 5208 5476 1632
Size: 575 1617 1384 268 1917
Fragments arranged by size:
1917 1617 1384 575 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5564
AflIII A'CryG_T
Cuts at: 1203 4660 5637 1203
Size: 3457 977 1327
Fragments arranged by size:
3457 1327 977
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 4490 4490
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1166 2462 1166
Size: 1296 4465
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5761
AlwNI CAG_nnn'CTG
Cuts at: 3504 5076 3504
Size: 1572 4189
ApaLI G'TGCA_C
Cuts at: 3818 4974 3818
Size: 1156 4605
ApoI r'AATT_y
Cuts at: 1493 2578 2789 3070 3296 3448 5557 1493
Size: 1085 211 281 226 152 2109 1697
Fragments arranged by size:
2109 1697 1085 281 226 211 152
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 2647 2999 3086 1870
Size: 73 704 352 87 4545
Fragments arranged by size:
4545 704 352 87 73
BamHI G'GATC_C
Cuts at: 3091 3458 3091
Size: 367 5394
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2382 3082 4537 618
Size: 325 217 627 595 700 1455 1842
Fragments arranged by size:
1842 1455 700 627 595 325 217
BanII G_rGCy'C
Cuts at: 1391 2061 2266 2325 2752 3080 1391
Size: 670 205 59 427 328 4072
Fragments arranged by size:
4072 670 427 328 205 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2516 2926 928
Size: 971 617 410 3763
Fragments arranged by size:
3763 971 617 410
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3952 3986 1903
Size: 34 2015 34 3678
Fragments arranged by size:
3678 2015 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3653 4863 3653
Size: 1210 4551
BclI T'GATC_A
Cuts at: 1548 2454 1548
Size: 906 4855
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4372 136
Size: 107 122 71 631 605 2700 1525
Fragments arranged by size:
2700 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2507 5756 2507
Size: 3249 2512
BlpI GC'TnA_GC
Cuts at: 3178 3178
Size: 5761
Bme1580I G_kGCm'C
Cuts at: 1790 3822 4978 1790
Size: 2032 1156 2573
Fragments arranged by size:
2573 2032 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5761
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2275 3042 3492
Size: 416 53 83 399 24 858 767 450
Cuts at: 3492 4450 442
Size: 958 1753
Fragments arranged by size:
1753 958 858 767 450 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4421 1239
Size: 3182 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3886 4751 5049 3886
Size: 865 298 4598
Fragments arranged by size:
4598 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4424 882
Size: 617 2925 2219
Fragments arranged by size:
2925 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3552 493
Size: 711 2348 2702
Fragments arranged by size:
2702 2348 711
BsaBI GATnn'nnATC
Cuts at: 2600 5755 2600
Size: 3155 2606
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3568 3950 4585 275
Size: 53 83 186 936 2035 382 635 1451
Fragments arranged by size:
2035 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4194 4592 4866 5013 5424 4194
Size: 398 274 147 411 4531
Fragments arranged by size:
4531 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5536
Fragments arranged by size:
5536 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5761
BseYI C'CCAG_C
Cuts at: 4964 5460 4964
Size: 496 5265
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5188
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3211 3972 5000 1540
Size: 275 186 1210 761 1028 2301
Fragments arranged by size:
2301 1210 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3822 3907 4978 3822
Size: 85 1071 4605
Fragments arranged by size:
4605 1071 85
BsmI GAATG_Cn'
Cuts at: 3272 3365 3272
Size: 93 5668
BsmBI CGTCTCn'nnnn_
Cuts at: 2487 2487
Size: 5761
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2266 2325 2752 3080 3822 3907
Size: 399 271 205 59 427 328 742 85
Cuts at: 3907 4978 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 427 399 328 271 205
85 59
BspEI T'CCGG_A
Cuts at: 5424 5424
Size: 5761
BspHI T'CATG_A
Cuts at: 2724 3650 5380 2724
Size: 926 1730 3105
Fragments arranged by size:
3105 1730 926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2217 2543 2833 3116 844
Size: 1373 326 290 283 3489
Fragments arranged by size:
3489 1373 326 290 283
BsrBI CCG'CTC
Cuts at: 1394 1823 2110 3648 1394
Size: 429 287 1538 3507
Fragments arranged by size:
3507 1538 429 287
BsrDI GCAATG_nn'
Cuts at: 65 1248 2949 4256 4430 65
Size: 1183 1701 1307 174 1396
Fragments arranged by size:
1701 1396 1307 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4405 4592 1700
Size: 110 2595 187 2869
Fragments arranged by size:
2869 2595 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5761
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5761
BssSI C'ACGA_G
Cuts at: 3821 4833 3821
Size: 1012 4749
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5761
BstBI TT'CG_AA
Cuts at: 4565 4565
Size: 5761
BstEII G'GTnAC_C
Cuts at: 1184 2428 1184
Size: 1244 4517
BstYI r'GATC_y
Cuts at: 1972 2507 3091 3458 3843 3860 5312 5398 5410
Size: 535 584 367 385 17 1452 86 12
Cuts at: 5410 5756 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 584 535 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4270
Fragments arranged by size:
4270 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5022
Fragments arranged by size:
5022 683 56
BtsI GCAGTG_nn'
Cuts at: 2869 3277 4071 4091 2869
Size: 408 794 20 4539
Fragments arranged by size:
4539 794 408 20
ClaI AT'CG_AT
Cuts at: 1554 2376 1554
Size: 822 4939
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5726
DraI TTT'AAA
Cuts at: 2415 3065 2415
Size: 650 5111
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4768 809
Size: 507 3452 1802
Fragments arranged by size:
3452 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2220 3208 4097
Size: 54 1425 50 275 18 390 988 889
Cuts at: 4097 5726 8
Size: 1629 43
Fragments arranged by size:
1629 1425 988 889 390 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3208 1537
Size: 275 1396 4090
Fragments arranged by size:
4090 1396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2183 2684 2747 3691 1353
Size: 830 501 63 944 3423
Fragments arranged by size:
3423 944 830 501 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4348 4722 4868 387
Size: 1064 138 2759 374 146 1280
Fragments arranged by size:
2759 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 3824 4421 5208 5476 1239
Size: 393 575 1617 597 787 268 1524
Fragments arranged by size:
1617 1524 787 597 575 393 268
EcoRI G'AATT_C
Cuts at: 2789 3070 3448 2789
Size: 281 378 5102
Fragments arranged by size:
5102 378 281
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2540 2540
Size: 5761
FspI TGC'GCA
Cuts at: 4267 4267
Size: 5761
HaeII r_GCGC'y
Cuts at: 1678 4908 1678
Size: 3230 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2839 2871 4402 4434 4476
Size: 32 135 32 1267 32 1531 32 42
Cuts at: 4476 4508 1373
Size: 32 2626
Fragments arranged by size:
2626 1531 1267 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2309 3105 3351 669
Size: 988 281 371 796 246 3079
Fragments arranged by size:
3079 988 796 371 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5761
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KpnI G_GTAC'C
Cuts at: 2386 3086 2386
Size: 700 5061
MfeI C'AATT_G
Cuts at: 3360 3360
Size: 5761
MluI A'CGCG_T
Cuts at: 5637 5637
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2808 4875 5059 1305
Size: 1503 2067 184 2007
Fragments arranged by size:
2067 2007 1503 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2222 10
Size: 54 1425 343 390 3549
Fragments arranged by size:
3549 1425 390 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2462 2723 3719 4078 4237 518
Size: 648 1296 261 996 359 159 2042
Fragments arranged by size:
2042 1296 996 648 359 261 159
MspA1I CmG'CkG
Cuts at: 1606 2623 3177 3854 5002 5247 1606
Size: 1017 554 677 1148 245 2120
Fragments arranged by size:
2120 1148 1017 677 554 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4270
Fragments arranged by size:
4270 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5761
NotI GC'GGCC_GC
Cuts at: 3208 3208
Size: 5761
NspI r_CATG'y
Cuts at: 1132 1229 2152 3119 4664 1132
Size: 97 923 967 1545 2229
Fragments arranged by size:
2229 1545 967 923 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5761
PciI A'CATG_T
Cuts at: 4660 4660
Size: 5761
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5761
PfoI T'CCnGG_A
Cuts at: 2784 2784
Size: 5761
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 5343 5375 3830
Size: 32 1481 32 4216
Fragments arranged by size:
4216 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 3331 3331
Size: 5761
PstI C_TGCA'G
Cuts at: 830 2540 3113 830
Size: 1710 573 3478
Fragments arranged by size:
3478 1710 573
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
SalI G'TCGA_C
Cuts at: 1936 3103 1936
Size: 1167 4594
SapI GCTCTTCn'nnn_
Cuts at: 2183 2183
Size: 5761
SbfI CC_TGCA'GG
Cuts at: 3113 3113
Size: 5761
ScaI AGT'ACT
Cuts at: 4009 5721 4009
Size: 1712 4049
SfcI C'TryA_G
Cuts at: 826 1045 2536 3109 4244 4925 5116 826
Size: 219 1491 573 1135 681 191 1471
Fragments arranged by size:
1491 1471 1135 681 573 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5761
SmaI CCC'GGG
Cuts at: 1872 3088 1872
Size: 1216 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3865 4766 5028 820
Size: 197 926 1922 901 262 1553
Fragments arranged by size:
1922 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3552 493
Size: 3059 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5761
SphI G_CATG'C
Cuts at: 1132 3119 1132
Size: 1987 3774
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5761
StyI C'CwwG_G
Cuts at: 513 1065 2004 3200 513
Size: 552 939 1196 3074
Fragments arranged by size:
3074 1196 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2452 3788 3805 3958 4143 4588 325
Size: 1255 872 1336 17 153 185 445 1498
Fragments arranged by size:
1498 1336 1255 872 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4007 5719 96
Size: 274 80 33 51 1443 2030 1712 138
Fragments arranged by size:
2030 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5721 5753 5721
Size: 32 5729
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5761
XbaI T'CTAG_A
Cuts at: 3097 3097
Size: 5761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2288 2792 2288
Size: 504 5257
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 3086 1870
Size: 1216 4545
XmnI GAAnn'nnTTC
Cuts at: 2523 2579 2780 3890 2523
Size: 56 201 1110 4394
Fragments arranged by size:
4394 1110 201 56
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3569 276
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI