Restriction map for
pFN21AB9836
(Circular) (Six-base) MAPSORT of: pFN21AB9836.seq Check: 1742 from: 1 to: 5758
pFN21AB9836 5758 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2581 2581
Size: 5758
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3101 1937
Size: 1164 4594
Acc65I G'GTAC_C
Cuts at: 3079 3079
Size: 5758
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2447 3821 5205 5473 1632
Size: 815 1374 1384 268 1917
Fragments arranged by size:
1917 1384 1374 815 268
AfeI AGC'GCT
Cuts at: 2064 2064
Size: 5758
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5561
AflIII A'CryG_T
Cuts at: 1203 4657 5634 1203
Size: 3454 977 1327
Fragments arranged by size:
3454 1327 977
AgeI A'CCGG_T
Cuts at: 4589 4589
Size: 5758
AhdI GACnn_n'nnGTC
Cuts at: 4487 4487
Size: 5758
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5758
AlwNI CAG_nnn'CTG
Cuts at: 2839 2963 3501 5073 2839
Size: 124 538 1572 3524
Fragments arranged by size:
3524 1572 538 124
ApaI G_GGCC'C
Cuts at: 2434 2434
Size: 5758
ApaLI G'TGCA_C
Cuts at: 3815 4971 3815
Size: 1156 4602
ApoI r'AATT_y
Cuts at: 1493 2327 2543 3067 3293 3445 5554 1493
Size: 834 216 524 226 152 2109 1697
Fragments arranged by size:
2109 1697 834 524 226 216 152
AseI AT'TA_AT
Cuts at: 160 1032 4312 160
Size: 872 3280 1606
Fragments arranged by size:
3280 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 2509 3083 1870
Size: 73 566 574 4545
Fragments arranged by size:
4545 574 566 73
AvrII C'CTAG_G
Cuts at: 2744 2744
Size: 5758
BamHI G'GATC_C
Cuts at: 3088 3455 3088
Size: 367 5391
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2593 3079 4534 618
Size: 325 217 627 806 486 1455 1842
Fragments arranged by size:
1842 1455 806 627 486 325 217
BanII G_rGCy'C
Cuts at: 1391 2374 2434 3077 1391
Size: 983 60 643 4072
Fragments arranged by size:
4072 983 643 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2031 928
Size: 971 132 4655
Fragments arranged by size:
4655 971 132
BbvCI CC'TCA_GC
Cuts at: 3009 3009
Size: 5758
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3949 3983 1903
Size: 34 2012 34 3678
Fragments arranged by size:
3678 2012 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3650 4860 3650
Size: 1210 4548
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2637 4369 136
Size: 107 122 71 631 605 965 1732 1525
Fragments arranged by size:
1732 1525 965 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5753 5753
Size: 5758
BlpI GC'TnA_GC
Cuts at: 3175 3175
Size: 5758
Bme1580I G_kGCm'C
Cuts at: 1790 2434 3819 4975 1790
Size: 644 1385 1156 2573
Fragments arranged by size:
2573 1385 1156 644
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3489 4447 442
Size: 416 53 83 399 24 2072 958 1753
Fragments arranged by size:
2072 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2388 2388
Size: 5758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2290 2508 2840 3014 4418 1239
Size: 1051 218 332 174 1404 2579
Fragments arranged by size:
2579 1404 1051 332 218 174
Bpu10I CC'TnA_GC
Cuts at: 3009 3009
Size: 5758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2651 2735 3883 4748 5046 2651
Size: 84 1148 865 298 3363
Fragments arranged by size:
3363 1148 865 298 84
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4421 882
Size: 617 2922 2219
Fragments arranged by size:
2922 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3549 493
Size: 711 2345 2702
Fragments arranged by size:
2702 2345 711
BsaBI GATnn'nnATC
Cuts at: 5752 5752
Size: 5758
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3565 3947 4582 275
Size: 53 83 186 936 2032 382 635 1451
Fragments arranged by size:
2032 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4191 4589 4863 5010 5421 4191
Size: 398 274 147 411 4528
Fragments arranged by size:
4528 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2813 2843 1178
Size: 30 165 30 1410 30 4093
Fragments arranged by size:
4093 1410 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 2732 2865 3026 3029 3032 1186
Size: 940 606 133 161 3 3 3912
Fragments arranged by size:
3912 940 606 161 133 3 3
BseYI C'CCAG_C
Cuts at: 2641 2903 2956 4961 5457 2641
Size: 262 53 2005 496 2942
Fragments arranged by size:
2942 2005 496 262 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2053 1163
Size: 573 317 4868
Fragments arranged by size:
4868 573 317
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3208 3969 4997 1540
Size: 275 186 1207 761 1028 2301
Fragments arranged by size:
2301 1207 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2374 3819 3904 4975 2374
Size: 1445 85 1071 3157
Fragments arranged by size:
3157 1445 1071 85
BsmI GAATG_Cn'
Cuts at: 3269 3362 3269
Size: 93 5665
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2374 2434 3077 3819 3904 4975 1391
Size: 399 584 60 643 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 643 584 399 85 60
BspEI T'CCGG_A
Cuts at: 5421 5421
Size: 5758
BspHI T'CATG_A
Cuts at: 3647 5377 3647
Size: 1730 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2581 2746 3113 844
Size: 1737 165 367 3489
Fragments arranged by size:
3489 1737 367 165
BsrBI CCG'CTC
Cuts at: 1394 1823 3645 1394
Size: 429 1822 3507
Fragments arranged by size:
3507 1822 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2483 2898 4253 4427 65
Size: 1183 1235 415 1355 174 1396
Fragments arranged by size:
1396 1355 1235 1183 415 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2075 4402 4589 1700
Size: 110 265 2327 187 2869
Fragments arranged by size:
2869 2327 265 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5758
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 3818 4830 3818
Size: 1012 4746
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5758
BstBI TT'CG_AA
Cuts at: 4562 4562
Size: 5758
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5758
BstYI r'GATC_y
Cuts at: 1972 3088 3455 3840 3857 5309 5395 5407 5753
Size: 1116 367 385 17 1452 86 12 346
Cuts at: 5753 1972
Size: 1977
Fragments arranged by size:
1977 1452 1116 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2241 2241
Size: 5758
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5206
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5019
Fragments arranged by size:
5019 683 56
BtsI GCAGTG_nn'
Cuts at: 2101 3274 4068 4088 2101
Size: 1173 794 20 3771
Fragments arranged by size:
3771 1173 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5723
DraI TTT'AAA
Cuts at: 3062 3062
Size: 5758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 809
Size: 507 3449 1802
Fragments arranged by size:
3449 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3205 4094 5723
Size: 54 1425 50 275 18 1375 889 1629
Cuts at: 5723 8
Size: 43
Fragments arranged by size:
1629 1425 1375 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3205 1537
Size: 275 1393 4090
Fragments arranged by size:
4090 1393 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2466 2603 3688 1353
Size: 1113 137 1085 3423
Fragments arranged by size:
3423 1113 1085 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4345 4719 4865 387
Size: 1064 138 2756 374 146 1280
Fragments arranged by size:
2756 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2372 2372
Size: 5758
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2290 2447 2508 2840 3014 3821 4418
Size: 393 658 157 61 332 174 807 597
Cuts at: 4418 5205 5473 1239
Size: 787 268 1524
Fragments arranged by size:
1524 807 787 658 597 393 332 268
174 157 61
EcoNI CCTnn'n_nnAGG
Cuts at: 3029 3029
Size: 5758
EcoO109I rG'GnC_Cy
Cuts at: 2431 3022 2431
Size: 591 5167
EcoRI G'AATT_C
Cuts at: 3067 3445 3067
Size: 378 5380
FspI TGC'GCA
Cuts at: 4264 4264
Size: 5758
HaeII r_GCGC'y
Cuts at: 1678 2066 4905 1678
Size: 388 2839 2531
Fragments arranged by size:
2839 2531 388
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2662 2694 4399 4431 4473
Size: 32 135 32 1090 32 1705 32 42
Cuts at: 4473 4505 1373
Size: 32 2626
Fragments arranged by size:
2626 1705 1090 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3102 3348 669
Size: 988 281 1164 246 3079
Fragments arranged by size:
3079 1164 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KpnI G_GTAC'C
Cuts at: 3083 3083
Size: 5758
MfeI C'AATT_G
Cuts at: 2856 3357 2856
Size: 501 5257
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4872 5056 1305
Size: 3567 184 2007
Fragments arranged by size:
3567 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3936
Fragments arranged by size:
3936 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 2385 3716 4075 4234 518
Size: 648 1185 34 1331 359 159 2042
Fragments arranged by size:
2042 1331 1185 648 359 159 34
MspA1I CmG'CkG
Cuts at: 1606 3008 3174 3851 4999 5244 1606
Size: 1402 166 677 1148 245 2120
Fragments arranged by size:
2120 1402 1148 677 245 166
NaeI GCC'GGC
Cuts at: 2077 2077
Size: 5758
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5206
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5758
NgoMIV G'CCGG_C
Cuts at: 2075 2075
Size: 5758
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 3116 4661 1132
Size: 97 1887 1545 2229
Fragments arranged by size:
2229 1887 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5758
PciI A'CATG_T
Cuts at: 4657 4657
Size: 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2951 2963 1108
Size: 1843 12 3903
Fragments arranged by size:
3903 1843 12
PfoI T'CCnGG_A
Cuts at: 2418 2418
Size: 5758
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3827 3859 5340 5372 3827
Size: 32 1481 32 4213
Fragments arranged by size:
4213 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5758
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PspOMI G'GGCC_C
Cuts at: 2430 2430
Size: 5758
PstI C_TGCA'G
Cuts at: 830 2096 2124 2709 3110 830
Size: 1266 28 585 401 3478
Fragments arranged by size:
3478 1266 585 401 28
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
PvuII CAG'CTG
Cuts at: 3008 3008
Size: 5758
SacI G_AGCT'C
Cuts at: 2374 2374
Size: 5758
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SapI GCTCTTCn'nnn_
Cuts at: 2466 2466
Size: 5758
SbfI CC_TGCA'GG
Cuts at: 3110 3110
Size: 5758
ScaI AGT'ACT
Cuts at: 4006 5718 4006
Size: 1712 4046
SfcI C'TryA_G
Cuts at: 826 1045 2092 2120 2705 3106 4241 4922 5113
Size: 219 1047 28 585 401 1135 681 191
Cuts at: 5113 826
Size: 1471
Fragments arranged by size:
1471 1135 1047 681 585 401 219 191
28
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5758
SmaI CCC'GGG
Cuts at: 1872 3085 1872
Size: 1213 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2666 2714 3862 4763 5025 820
Size: 197 926 723 48 1148 901 262 1553
Fragments arranged by size:
1553 1148 926 901 723 262 197 48
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 2222 152
Size: 2070 3688
SphI G_CATG'C
Cuts at: 1132 3116 1132
Size: 1984 3774
SspI AAT'ATT
Cuts at: 5 52 3039 3682 5
Size: 47 2987 643 2081
Fragments arranged by size:
2987 2081 643 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5758
StyI C'CwwG_G
Cuts at: 513 1065 2139 2744 3197 513
Size: 552 1074 605 453 3074
Fragments arranged by size:
3074 1074 605 552 453
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2345 3785 3802 3955 4140 4585 325
Size: 1255 765 1440 17 153 185 445 1498
Fragments arranged by size:
1498 1440 1255 765 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2148 2809 4004
Size: 274 80 33 51 1443 171 661 1195
Cuts at: 4004 5716 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1195 661 274 171 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5718 5750 5718
Size: 32 5726
Tth111I GACn'n_nGTC
Cuts at: 1316 2894 1316
Size: 1578 4180
XbaI T'CTAG_A
Cuts at: 3094 3094
Size: 5758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2900 2900
Size: 5758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5758
XmaI C'CCGG_G
Cuts at: 1870 3083 1870
Size: 1213 4545
XmnI GAAnn'nnTTC
Cuts at: 3887 3887
Size: 5758
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BfrBI BsiWI BsmBI BstXI
BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI