Restriction map for
pFN21AB9835
(Circular) (Six-base) MAPSORT of: pFN21AB9835.seq Check: 173 from: 1 to: 5755
pFN21AB9835 5755 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2077 2077
Size: 5755
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2232 3098 1937
Size: 295 866 4594
Fragments arranged by size:
4594 866 295
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2372 3818 5202 5470 1632
Size: 740 1446 1384 268 1917
Fragments arranged by size:
1917 1446 1384 740 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 4654 5631 1203
Size: 3451 977 1327
Fragments arranged by size:
3451 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 2029 1166
Size: 863 4892
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5755
AlwNI CAG_nnn'CTG
Cuts at: 2091 3498 5070 2091
Size: 1407 1572 2776
Fragments arranged by size:
2776 1572 1407
ApaI G_GGCC'C
Cuts at: 2863 2863
Size: 5755
ApaLI G'TGCA_C
Cuts at: 3812 4968 3812
Size: 1156 4599
ApoI r'AATT_y
Cuts at: 1493 2050 2935 3064 3290 3442 5551 1493
Size: 557 885 129 226 152 2109 1697
Fragments arranged by size:
2109 1697 885 557 226 152 129
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 3080 1870
Size: 73 1137 4545
Fragments arranged by size:
4545 1137 73
BamHI G'GATC_C
Cuts at: 2613 3085 3452 2613
Size: 472 367 4916
Fragments arranged by size:
4916 472 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3076 4531 618
Size: 325 217 627 1289 1455 1842
Fragments arranged by size:
1842 1455 1289 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2863 3074 1391
Size: 1472 211 4072
Fragments arranged by size:
4072 1472 211
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4784
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2833 2867 3946 3980 1903
Size: 34 896 34 1079 34 3678
Fragments arranged by size:
3678 1079 896 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3647 4857 3647
Size: 1210 4545
BclI T'GATC_A
Cuts at: 1548 2130 1548
Size: 582 5173
BfrBI ATG'CAT
Cuts at: 2971 2971
Size: 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4366 136
Size: 107 122 71 631 605 2694 1525
Fragments arranged by size:
2694 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 2159 3172 2159
Size: 1013 4742
Bme1580I G_kGCm'C
Cuts at: 1790 2863 3816 4972 1790
Size: 1073 953 1156 2573
Fragments arranged by size:
2573 1156 1073 953
BmgBI CAC'GTC
Cuts at: 1318 3002 1318
Size: 1684 4071
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3486 4444 442
Size: 416 53 83 399 24 2069 958 1753
Fragments arranged by size:
2069 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4415 1239
Size: 3176 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2939 3880 4745 5043 2939
Size: 941 865 298 3651
Fragments arranged by size:
3651 941 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2347 4418 882
Size: 617 848 2071 2219
Fragments arranged by size:
2219 2071 848 617
BsaAI yAC'GTr
Cuts at: 493 1204 3546 493
Size: 711 2342 2702
Fragments arranged by size:
2702 2342 711
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3562 3944 4579 275
Size: 53 83 186 936 2029 382 635 1451
Fragments arranged by size:
2029 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4188 4586 4860 5007 5418 4188
Size: 398 274 147 411 4525
Fragments arranged by size:
4525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5530
Fragments arranged by size:
5530 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5755
BseYI C'CCAG_C
Cuts at: 2167 2484 4958 5454 2167
Size: 317 2474 496 2468
Fragments arranged by size:
2474 2468 496 317
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2215 2857 3205 3966 4994 1540
Size: 275 186 214 642 348 761 1028 2301
Fragments arranged by size:
2301 1028 761 642 348 275 214 186
BsiHKAI G_wGCw'C
Cuts at: 3816 3901 4972 3816
Size: 85 1071 4599
Fragments arranged by size:
4599 1071 85
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2863 3074 3816 3901 4972 1391
Size: 399 1073 211 742 85 1071 2174
Fragments arranged by size:
2174 1073 1071 742 399 211 85
BspEI T'CCGG_A
Cuts at: 5418 5418
Size: 5755
BspHI T'CATG_A
Cuts at: 2805 3644 5374 2805
Size: 839 1730 3186
Fragments arranged by size:
3186 1730 839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2077 2244 3110 844
Size: 1233 167 866 3489
Fragments arranged by size:
3489 1233 866 167
BsrBI CCG'CTC
Cuts at: 1394 1823 2217 3642 1394
Size: 429 394 1425 3507
Fragments arranged by size:
3507 1425 429 394
BsrDI GCAATG_nn'
Cuts at: 65 1248 4250 4424 65
Size: 1183 3002 174 1396
Fragments arranged by size:
3002 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4399 4586 1700
Size: 110 2589 187 2869
Fragments arranged by size:
2869 2589 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5755
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 3815 4827 3815
Size: 1012 4743
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5755
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstXI CCAn_nnnn'nTGG
Cuts at: 2031 2031
Size: 5755
BstYI r'GATC_y
Cuts at: 1972 2020 2613 2958 3085 3452 3837 3854 5306
Size: 48 593 345 127 367 385 17 1452
Cuts at: 5306 5392 5404 5750 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 593 385 367 346 345 127
86 48 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2944 513
Size: 552 1879 3324
Fragments arranged by size:
3324 1879 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2119 487
Size: 683 56 893 4123
Fragments arranged by size:
4123 893 683 56
BtsI GCAGTG_nn'
Cuts at: 3271 4065 4085 3271
Size: 794 20 4941
Fragments arranged by size:
4941 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4762 809
Size: 507 3446 1802
Fragments arranged by size:
3446 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2212 2253 2415
Size: 54 1425 50 275 18 382 41 162
Cuts at: 2415 2854 2929 3202 4091 5720 8
Size: 439 75 273 889 1629 43
Fragments arranged by size:
1629 1425 889 439 382 275 273 162
75 54 50 43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 2212 2854 3202 1537
Size: 275 400 642 348 4090
Fragments arranged by size:
4090 642 400 348 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3049 3685 1353
Size: 1696 636 3423
Fragments arranged by size:
3423 1696 636
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2516 2614 4342 4716 4862 387
Size: 1064 138 927 98 1728 374 146 1280
Fragments arranged by size:
1728 1280 1064 927 374 146 138 98
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2372 3818 4415 5202 5470 1239
Size: 393 740 1446 597 787 268 1524
Fragments arranged by size:
1524 1446 787 740 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2333 2333
Size: 5755
EcoO109I rG'GnC_Cy
Cuts at: 2572 2811 2860 2572
Size: 239 49 5467
Fragments arranged by size:
5467 239 49
EcoRI G'AATT_C
Cuts at: 3064 3442 3064
Size: 378 5377
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2473 3010 2473
Size: 537 5218
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 2842 4902 1678
Size: 1164 2060 2531
Fragments arranged by size:
2531 2060 1164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2605 2637 4396 4428 4470
Size: 32 135 32 1033 32 1759 32 42
Cuts at: 4470 4502 1373
Size: 32 2626
Fragments arranged by size:
2626 1759 1033 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2362 3099 3345 669
Size: 988 281 424 737 246 3079
Fragments arranged by size:
3079 988 737 424 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3072 4869 5053 1305
Size: 1767 1797 184 2007
Fragments arranged by size:
2007 1797 1767 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2417 10
Size: 54 1425 343 585 3348
Fragments arranged by size:
3348 1425 585 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2029 2060 2329 2399 2804 3713 4072
Size: 648 863 31 269 70 405 909 359
Cuts at: 4072 4231 518
Size: 159 2042
Fragments arranged by size:
2042 909 863 648 405 359 269 159
70 31
MspA1I CmG'CkG
Cuts at: 1606 2647 3171 3848 4996 5241 1606
Size: 1041 524 677 1148 245 2120
Fragments arranged by size:
2120 1148 1041 677 524 245
NcoI C'CATG_G
Cuts at: 513 1065 2944 513
Size: 552 1879 3324
Fragments arranged by size:
3324 1879 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NsiI A_TGCA'T
Cuts at: 2973 2973
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 2065 3113 4658 1132
Size: 97 836 1048 1545 2229
Fragments arranged by size:
2229 1545 1048 836 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5755
PciI A'CATG_T
Cuts at: 4654 4654
Size: 5755
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5755
PfoI T'CCnGG_A
Cuts at: 2823 2823
Size: 5755
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2572 2811 2572
Size: 239 5516
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PspOMI G'GGCC_C
Cuts at: 2859 2859
Size: 5755
PstI C_TGCA'G
Cuts at: 830 2074 3107 830
Size: 1244 1033 3478
Fragments arranged by size:
3478 1244 1033
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SexAI A'CCwGG_T
Cuts at: 2781 2781
Size: 5755
SfcI C'TryA_G
Cuts at: 826 1045 2070 2368 3103 4238 4919 5110 826
Size: 219 1025 298 735 1135 681 191 1471
Fragments arranged by size:
1471 1135 1025 735 681 298 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 3082 1872
Size: 1210 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2954 3859 4760 5022 820
Size: 197 926 1011 905 901 262 1553
Fragments arranged by size:
1553 1011 926 905 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 2065 3113 1132
Size: 933 1048 3774
Fragments arranged by size:
3774 1048 933
SspI AAT'ATT
Cuts at: 5 52 2014 3679 5
Size: 47 1962 1665 2081
Fragments arranged by size:
2081 1962 1665 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5755
StyI C'CwwG_G
Cuts at: 513 1065 2250 2298 2944 3194 513
Size: 552 1185 48 646 250 3074
Fragments arranged by size:
3074 1185 646 552 250 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3782 3799 3952 4137 4582 325
Size: 1255 2202 17 153 185 445 1498
Fragments arranged by size:
2202 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4001 5713 96
Size: 274 80 33 51 1443 2024 1712 138
Fragments arranged by size:
2024 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5715 5747 5715
Size: 32 5723
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5755
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2878 2878
Size: 5755
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 3080 1870
Size: 1210 4545
XmnI GAAnn'nnTTC
Cuts at: 3884 3884
Size: 5755
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI