Restriction map for
pFN21AB9834
(Circular) (Six-base) MAPSORT of: pFN21AB9834.seq Check: 9340 from: 1 to: 5743
pFN21AB9834 5743 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2170 2170
Size: 5743
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2952 3553 278
Size: 53 83 186 936 1416 601 2468
Fragments arranged by size:
2468 1416 936 601 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3086 1937
Size: 1149 4594
Acc65I G'GTAC_C
Cuts at: 3064 3064
Size: 5743
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2630 3806 5190 5458 1632
Size: 998 1176 1384 268 1917
Fragments arranged by size:
1917 1384 1176 998 268
AfeI AGC'GCT
Cuts at: 2960 2960
Size: 5743
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5546
AflIII A'CryG_T
Cuts at: 1203 4642 5619 1203
Size: 3439 977 1327
Fragments arranged by size:
3439 1327 977
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 4472 4472
Size: 5743
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5743
AlwNI CAG_nnn'CTG
Cuts at: 2200 3486 5058 2200
Size: 1286 1572 2885
Fragments arranged by size:
2885 1572 1286
ApaI G_GGCC'C
Cuts at: 2134 2134
Size: 5743
ApaLI G'TGCA_C
Cuts at: 2278 2597 3800 4956 2278
Size: 319 1203 1156 3065
Fragments arranged by size:
3065 1203 1156 319
ApoI r'AATT_y
Cuts at: 1493 3052 3278 3430 5539 1493
Size: 1559 226 152 2109 1697
Fragments arranged by size:
2109 1697 1559 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 2127 2930 3068 1870
Size: 73 184 803 138 4545
Fragments arranged by size:
4545 803 184 138 73
AvrII C'CTAG_G
Cuts at: 2404 2404
Size: 5743
BamHI G'GATC_C
Cuts at: 3073 3440 3073
Size: 367 5376
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2462 2833 3064 4519 618
Size: 325 217 627 675 371 231 1455 1842
Fragments arranged by size:
1842 1455 675 627 371 325 231 217
BanII G_rGCy'C
Cuts at: 1391 2134 2325 2754 3062 1391
Size: 743 191 429 308 4072
Fragments arranged by size:
4072 743 429 308 191
BbeI G_GCGC'C
Cuts at: 2466 2837 2466
Size: 371 5372
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2905 928
Size: 971 1006 3766
Fragments arranged by size:
3766 1006 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3934 3968 1903
Size: 34 50 34 1913 34 3678
Fragments arranged by size:
3678 1913 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3635 4845 3635
Size: 1210 4533
BclI T'GATC_A
Cuts at: 1548 2628 1548
Size: 1080 4663
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2149 4354 136
Size: 107 122 71 631 605 477 2205 1525
Fragments arranged by size:
2205 1525 631 605 477 122 107 71
BglII A'GATC_T
Cuts at: 2686 5738 2686
Size: 3052 2691
BlpI GC'TnA_GC
Cuts at: 2319 3160 2319
Size: 841 4902
Bme1580I G_kGCm'C
Cuts at: 1790 2134 2282 2601 3804 4960 1790
Size: 344 148 319 1203 1156 2573
Fragments arranged by size:
2573 1203 1156 344 319 148
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5743
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2182 3474 4432
Size: 416 53 83 399 24 765 1292 958
Cuts at: 4432 442
Size: 1753
Fragments arranged by size:
1753 1292 958 765 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2229 2532 2986 4403 1239
Size: 990 303 454 1417 2579
Fragments arranged by size:
2579 1417 990 454 303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2525 3868 4733 5031 2525
Size: 1343 865 298 3237
Fragments arranged by size:
3237 1343 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2962 4406 882
Size: 617 1463 1444 2219
Fragments arranged by size:
2219 1463 1444 617
BsaAI yAC'GTr
Cuts at: 493 1204 3534 493
Size: 711 2330 2702
Fragments arranged by size:
2702 2330 711
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2463 2774 2834 2949
Size: 53 83 186 936 930 311 60 115
Cuts at: 2949 3550 3932 4567 275
Size: 601 382 635 1451
Fragments arranged by size:
1451 936 930 635 601 382 311 186
115 83 60 53
BsaWI w'CCGG_w
Cuts at: 2037 4176 4574 4848 4995 5406 2037
Size: 2139 398 274 147 411 2374
Fragments arranged by size:
2374 2139 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2168 2198 2754 2784 1178
Size: 30 165 30 765 30 556 30 4137
Fragments arranged by size:
4137 765 556 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5743
BseYI C'CCAG_C
Cuts at: 2200 2972 4946 5442 2200
Size: 772 1974 496 2501
Fragments arranged by size:
2501 1974 772 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5170
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3193 3954 4982 1540
Size: 275 186 1192 761 1028 2301
Fragments arranged by size:
2301 1192 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2165 2282 2325 2601 2754 3804 3889 4960 2165
Size: 117 43 276 153 1050 85 1071 2948
Fragments arranged by size:
2948 1071 1050 276 153 117 85 43
BsmI GAATG_Cn'
Cuts at: 3254 3347 3254
Size: 93 5650
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2134 2165 2282 2325 2601 2754 3062
Size: 399 344 31 117 43 276 153 308
Cuts at: 3062 3804 3889 4960 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 344 308 276 153
117 85 43 31
BspEI T'CCGG_A
Cuts at: 2037 5406 2037
Size: 3369 2374
BspHI T'CATG_A
Cuts at: 3632 5362 3632
Size: 1730 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2104 2170 2895 3098 844
Size: 1260 66 725 203 3489
Fragments arranged by size:
3489 1260 725 203 66
BsrBI CCG'CTC
Cuts at: 1394 1823 2851 3630 1394
Size: 429 1028 779 3507
Fragments arranged by size:
3507 1028 779 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4238 4412 65
Size: 1183 2990 174 1396
Fragments arranged by size:
2990 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2148 2468 4387 4574 1700
Size: 110 338 320 1919 187 2869
Fragments arranged by size:
2869 1919 338 320 187 110
BsrGI T'GTAC_A
Cuts at: 96 2226 96
Size: 2130 3613
BssHII G'CGCG_C
Cuts at: 1925 2994 1925
Size: 1069 4674
BssSI C'ACGA_G
Cuts at: 3803 4815 3803
Size: 1012 4731
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5743
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstXI CCAn_nnnn'nTGG
Cuts at: 2419 2419
Size: 5743
BstYI r'GATC_y
Cuts at: 1972 2686 3013 3073 3440 3825 3842 5294 5380
Size: 714 327 60 367 385 17 1452 86
Cuts at: 5380 5392 5738 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 714 385 367 346 327 86
60 17 12
Bsu36I CC'TnA_GG
Cuts at: 2478 2478
Size: 5743
BtgI C'CryG_G
Cuts at: 513 1065 2135 2242 513
Size: 552 1070 107 4014
Fragments arranged by size:
4014 1070 552 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5004
Fragments arranged by size:
5004 683 56
BtsI GCAGTG_nn'
Cuts at: 2500 3259 4053 4073 2500
Size: 759 794 20 4170
Fragments arranged by size:
4170 794 759 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5708
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4750 809
Size: 507 3434 1802
Fragments arranged by size:
3434 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2256 2470 3190
Size: 54 1425 50 275 18 426 214 720
Cuts at: 3190 4079 5708 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 720 426 275 214 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3190 1537
Size: 275 1378 4090
Fragments arranged by size:
4090 1378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 2579 3673 1353
Size: 774 452 1094 3423
Fragments arranged by size:
3423 1094 774 452
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2320 4330 4704 4850 387
Size: 1064 138 731 2010 374 146 1280
Fragments arranged by size:
2010 1280 1064 731 374 146 138
EcoICRI GAG'CTC
Cuts at: 2323 2752 2323
Size: 429 5314
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2229 2532 2630 2986 3806 4403 5190
Size: 393 597 303 98 356 820 597 787
Cuts at: 5190 5458 1239
Size: 268 1524
Fragments arranged by size:
1524 820 787 597 597 393 356 303
268 98
EcoNI CCTnn'n_nnAGG
Cuts at: 2721 2721
Size: 5743
EcoO109I rG'GnC_Cy
Cuts at: 2131 2131
Size: 5743
EcoRI G'AATT_C
Cuts at: 3052 3430 3052
Size: 378 5365
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 2466 2837 2941 2962 4890 1678
Size: 788 371 104 21 1928 2531
Fragments arranged by size:
2531 1928 788 371 104 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3005 3037 4384 4416 4458
Size: 32 135 32 1433 32 1347 32 42
Cuts at: 4458 4490 1373
Size: 32 2626
Fragments arranged by size:
2626 1433 1347 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3087 3333 669
Size: 988 281 1149 246 3079
Fragments arranged by size:
3079 1149 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5743
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KasI G'GCGC_C
Cuts at: 2462 2833 2462
Size: 371 5372
KpnI G_GTAC'C
Cuts at: 3068 3068
Size: 5743
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2661 4857 5041 1305
Size: 1356 2196 184 2007
Fragments arranged by size:
2196 2007 1356 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2258 10
Size: 54 1425 343 426 3495
Fragments arranged by size:
3495 1425 426 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 3701 4060 4219 518
Size: 648 1251 1284 359 159 2042
Fragments arranged by size:
2042 1284 1251 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2137 2244 2906 3159 3836 4984 5229 1606
Size: 531 107 662 253 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 662 531 253 245 107
NaeI GCC'GGC
Cuts at: 2150 2470 2150
Size: 320 5423
NarI GG'CG_CC
Cuts at: 2463 2834 2463
Size: 371 5372
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5191
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5743
NgoMIV G'CCGG_C
Cuts at: 2148 2468 2148
Size: 320 5423
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5743
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 3101 4646 1132
Size: 97 1872 1545 2229
Fragments arranged by size:
2229 1872 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5743
PciI A'CATG_T
Cuts at: 4642 4642
Size: 5743
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2172 1108
Size: 1064 4679
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 3313 3313
Size: 5743
PspOMI G'GGCC_C
Cuts at: 2130 2130
Size: 5743
PstI C_TGCA'G
Cuts at: 830 2078 2196 3095 830
Size: 1248 118 899 3478
Fragments arranged by size:
3478 1248 899 118
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
SacI G_AGCT'C
Cuts at: 2325 2754 2325
Size: 429 5314
SacII CC_GC'GG
Cuts at: 2138 2245 2138
Size: 107 5636
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SapI GCTCTTCn'nnn_
Cuts at: 2579 2579
Size: 5743
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 2546 3991 5703 2546
Size: 1445 1712 2586
Fragments arranged by size:
2586 1712 1445
SfcI C'TryA_G
Cuts at: 826 1045 2074 2192 3091 4226 4907 5098 826
Size: 219 1029 118 899 1135 681 191 1471
Fragments arranged by size:
1471 1135 1029 899 681 219 191 118
SfoI GGC'GCC
Cuts at: 2464 2835 2464
Size: 371 5372
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 2932 3070 1872
Size: 1060 138 4545
Fragments arranged by size:
4545 1060 138
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2540 3847 4748 5010 820
Size: 197 926 597 1307 901 262 1553
Fragments arranged by size:
1553 1307 926 901 597 262 197
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 3101 1132
Size: 1969 3774
SspI AAT'ATT
Cuts at: 5 52 3667 5
Size: 47 3615 2081
Fragments arranged by size:
3615 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5743
StyI C'CwwG_G
Cuts at: 513 1065 2404 2941 3182 513
Size: 552 1339 537 241 3074
Fragments arranged by size:
3074 1339 552 537 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2750 3770 3787 3940 4125 4570 325
Size: 1255 1170 1020 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1170 1020 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2226 2544 3989
Size: 274 80 33 51 1443 249 318 1445
Cuts at: 3989 5701 96
Size: 1712 138
Fragments arranged by size:
1712 1445 1443 318 274 249 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2168 2200 2754 2786 5703 5735 2168
Size: 32 554 32 2917 32 2176
Fragments arranged by size:
2917 2176 554 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5743
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2456 2456
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5743
XmaI C'CCGG_G
Cuts at: 1870 2930 3068 1870
Size: 1060 138 4545
Fragments arranged by size:
4545 1060 138
XmnI GAAnn'nnTTC
Cuts at: 3872 3872
Size: 5743
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2950 3551 276
Size: 53 83 186 936 1416 601 2468
Fragments arranged by size:
2468 1416 936 601 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI
BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII
RsrII SanDI SexAI SfiI SrfI SwaI