Restriction map for
pFN21AB9833
(Circular) (Six-base) MAPSORT of: pFN21AB9833.seq Check: 6948 from: 1 to: 5740
pFN21AB9833 5740 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3550 278
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2176 3083 1937
Size: 239 907 4594
Fragments arranged by size:
4594 907 239
Acc65I G'GTAC_C
Cuts at: 3061 3061
Size: 5740
AclI AA'CG_TT
Cuts at: 1563 3039 3867 4240 1563
Size: 1476 828 373 3063
Fragments arranged by size:
3063 1476 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3803 5187 5455 1632
Size: 2171 1384 268 1917
Fragments arranged by size:
2171 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2111 820
Size: 197 1094 4449
Fragments arranged by size:
4449 1094 197
AflIII A'CryG_T
Cuts at: 1203 2074 4639 5616 1203
Size: 871 2565 977 1327
Fragments arranged by size:
2565 1327 977 871
AgeI A'CCGG_T
Cuts at: 4571 4571
Size: 5740
AhdI GACnn_n'nnGTC
Cuts at: 4469 4469
Size: 5740
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5740
AlwNI CAG_nnn'CTG
Cuts at: 3483 5055 3483
Size: 1572 4168
ApaLI G'TGCA_C
Cuts at: 3797 4953 3797
Size: 1156 4584
ApoI r'AATT_y
Cuts at: 1493 2032 2822 2980 3049 3275 3427 5536 1493
Size: 539 790 158 69 226 152 2109 1697
Fragments arranged by size:
2109 1697 790 539 226 158 152 69
AseI AT'TA_AT
Cuts at: 160 1032 4294 160
Size: 872 3262 1606
Fragments arranged by size:
3262 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
AvaI C'yCGr_G
Cuts at: 1870 1943 3065 1870
Size: 73 1122 4545
Fragments arranged by size:
4545 1122 73
BamHI G'GATC_C
Cuts at: 3070 3437 3070
Size: 367 5373
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3061 4516 618
Size: 325 217 627 1274 1455 1842
Fragments arranged by size:
1842 1455 1274 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3059 1391
Size: 1668 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4769
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3931 3965 1903
Size: 34 50 34 1910 34 3678
Fragments arranged by size:
3678 1910 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2148 3632 4842 2148
Size: 1484 1210 3046
Fragments arranged by size:
3046 1484 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5740
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4351 136
Size: 107 122 71 631 605 2679 1525
Fragments arranged by size:
2679 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5735 5735
Size: 5740
BlpI GC'TnA_GC
Cuts at: 3157 3157
Size: 5740
Bme1580I G_kGCm'C
Cuts at: 1790 2133 3801 4957 1790
Size: 343 1668 1156 2573
Fragments arranged by size:
2573 1668 1156 343
BmgBI CAC'GTC
Cuts at: 1318 2075 1318
Size: 757 4983
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2195 3471 4429
Size: 416 53 83 399 24 778 1276 958
Cuts at: 4429 442
Size: 1753
Fragments arranged by size:
1753 1276 958 778 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2891 4400 1239
Size: 1652 1509 2579
Fragments arranged by size:
2579 1652 1509
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3865 4730 5028 3865
Size: 865 298 4577
Fragments arranged by size:
4577 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4403 882
Size: 617 2904 2219
Fragments arranged by size:
2904 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2344 2612 3531 493
Size: 711 1140 268 919 2702
Fragments arranged by size:
2702 1140 919 711 268
BsaBI GATnn'nnATC
Cuts at: 5734 5734
Size: 5740
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3547 3929 4564 275
Size: 53 83 186 936 2014 382 635 1451
Fragments arranged by size:
2014 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4173 4571 4845 4992 5403 4173
Size: 398 274 147 411 4510
Fragments arranged by size:
4510 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5515
Fragments arranged by size:
5515 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5740
BseYI C'CCAG_C
Cuts at: 2437 2745 4943 5439 2437
Size: 308 2198 496 2738
Fragments arranged by size:
2738 2198 496 308
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5167
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3190 3951 4979 1540
Size: 275 186 1189 761 1028 2301
Fragments arranged by size:
2301 1189 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3801 3886 4957 3801
Size: 85 1071 4584
Fragments arranged by size:
4584 1071 85
BsmI GAATG_Cn'
Cuts at: 3251 3344 3251
Size: 93 5647
BsmBI CGTCTCn'nnnn_
Cuts at: 2065 2065
Size: 5740
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 3059 3801 3886 4957 1391
Size: 399 343 926 742 85 1071 2174
Fragments arranged by size:
2174 1071 926 742 399 343 85
BspEI T'CCGG_A
Cuts at: 5403 5403
Size: 5740
BspHI T'CATG_A
Cuts at: 3629 5359 3629
Size: 1730 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3095 844
Size: 2251 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3627 1394
Size: 429 1804 3507
Fragments arranged by size:
3507 1804 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4235 4409 65
Size: 1183 2987 174 1396
Fragments arranged by size:
2987 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4384 4571 1700
Size: 110 2574 187 2869
Fragments arranged by size:
2869 2574 187 110
BsrGI T'GTAC_A
Cuts at: 96 2052 96
Size: 1956 3784
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5740
BssSI C'ACGA_G
Cuts at: 3800 4812 3800
Size: 1012 4728
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5740
BstBI TT'CG_AA
Cuts at: 4544 4544
Size: 5740
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5740
BstYI r'GATC_y
Cuts at: 1972 2269 3070 3437 3822 3839 5291 5377 5389
Size: 297 801 367 385 17 1452 86 12
Cuts at: 5389 5735 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 801 385 367 346 297 86
17 12
BstZ17I GTA'TAC
Cuts at: 2177 2177
Size: 5740
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2448 487
Size: 683 56 1222 3779
Fragments arranged by size:
3779 1222 683 56
BtsI GCAGTG_nn'
Cuts at: 2538 3256 4050 4070 2538
Size: 718 794 20 4208
Fragments arranged by size:
4208 794 718 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5740
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2209 2244 529
Size: 35 1645 35 4025
Fragments arranged by size:
4025 1645 35 35
DraI TTT'AAA
Cuts at: 3044 3044
Size: 5740
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4747 809
Size: 507 3431 1802
Fragments arranged by size:
3431 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2590 3187 4076
Size: 54 1425 50 275 18 760 597 889
Cuts at: 4076 5705 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 760 597 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3187 1537
Size: 275 1375 4090
Fragments arranged by size:
4090 1375 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2677 3670 1353
Size: 1324 993 3423
Fragments arranged by size:
3423 1324 993
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4327 4701 4847 387
Size: 1064 138 2738 374 146 1280
Fragments arranged by size:
2738 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2891 3803 4400 5187 5455 1239
Size: 393 1259 912 597 787 268 1524
Fragments arranged by size:
1524 1259 912 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3049 3427 3049
Size: 378 5362
FspI TGC'GCA
Cuts at: 4246 4246
Size: 5740
HaeII r_GCGC'y
Cuts at: 1678 4887 1678
Size: 3209 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2426 2458 4381 4413 4455
Size: 32 135 32 854 32 1923 32 42
Cuts at: 4455 4487 1373
Size: 32 2626
Fragments arranged by size:
2626 1923 854 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2080 3084 3330 669
Size: 988 281 142 1004 246 3079
Fragments arranged by size:
3079 1004 988 281 246 142
HindIII A'AGCT_T
Cuts at: 748 2060 748
Size: 1312 4428
HpaI GTT'AAC
Cuts at: 3330 3330
Size: 5740
KpnI G_GTAC'C
Cuts at: 3065 3065
Size: 5740
MfeI C'AATT_G
Cuts at: 3339 3339
Size: 5740
MluI A'CGCG_T
Cuts at: 5616 5616
Size: 5740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2355 4854 5038 1305
Size: 1050 2499 184 2007
Fragments arranged by size:
2499 2007 1050 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3918
Fragments arranged by size:
3918 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3698 4057 4216 518
Size: 648 2532 359 159 2042
Fragments arranged by size:
2532 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3156 3833 4981 5226 1606
Size: 1550 677 1148 245 2120
Fragments arranged by size:
2120 1550 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5188
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5740
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5740
NotI GC'GGCC_GC
Cuts at: 3187 3187
Size: 5740
NspI r_CATG'y
Cuts at: 1132 1229 3001 3098 4643 1132
Size: 97 1772 97 1545 2229
Fragments arranged by size:
2229 1772 1545 97 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5740
PciI A'CATG_T
Cuts at: 4639 4639
Size: 5740
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5740
PmeI GTTT'AAAC
Cuts at: 3044 3044
Size: 5740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 5322 5354 3809
Size: 32 1481 32 4195
Fragments arranged by size:
4195 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5740
PsiI TTA'TAA
Cuts at: 3310 3310
Size: 5740
PstI C_TGCA'G
Cuts at: 830 3092 830
Size: 2262 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5740
SalI G'TCGA_C
Cuts at: 1936 3082 1936
Size: 1146 4594
SbfI CC_TGCA'GG
Cuts at: 3092 3092
Size: 5740
ScaI AGT'ACT
Cuts at: 3988 5700 3988
Size: 1712 4028
SfcI C'TryA_G
Cuts at: 826 1045 3088 4223 4904 5095 826
Size: 219 2043 1135 681 191 1471
Fragments arranged by size:
2043 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5740
SmaI CCC'GGG
Cuts at: 1872 3067 1872
Size: 1195 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2111 3844 4745 5007 820
Size: 197 926 168 1733 901 262 1553
Fragments arranged by size:
1733 1553 926 901 262 197 168
SnaBI TAC'GTA
Cuts at: 493 3531 493
Size: 3038 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5740
SphI G_CATG'C
Cuts at: 1132 3098 1132
Size: 1966 3774
SspI AAT'ATT
Cuts at: 5 52 3664 5
Size: 47 3612 2081
Fragments arranged by size:
3612 2081 47
StuI AGG'CCT
Cuts at: 1517 2949 1517
Size: 1432 4308
StyI C'CwwG_G
Cuts at: 513 1065 2817 3179 513
Size: 552 1752 362 3074
Fragments arranged by size:
3074 1752 552 362
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3767 3784 3937 4122 4567 325
Size: 1255 2187 17 153 185 445 1498
Fragments arranged by size:
2187 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2052 2910 3986
Size: 274 80 33 51 1443 75 858 1076
Cuts at: 3986 5698 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1076 858 274 138 80 75
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5700 5732 5700
Size: 32 5708
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5740
XbaI T'CTAG_A
Cuts at: 3076 3076
Size: 5740
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5740
XmaI C'CCGG_G
Cuts at: 1870 3065 1870
Size: 1195 4545
XmnI GAAnn'nnTTC
Cuts at: 2106 3869 2106
Size: 1763 3977
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3548 276
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI