Restriction map for
pFN21AB9830
(Circular) (Six-base) MAPSORT of: pFN21AB9830.seq Check: 9704 from: 1 to: 5731
pFN21AB9830 5731 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3541 278
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2016 3074 1937
Size: 79 1058 4594
Fragments arranged by size:
4594 1058 79
Acc65I G'GTAC_C
Cuts at: 3052 3052
Size: 5731
AclI AA'CG_TT
Cuts at: 1563 3858 4231 1563
Size: 2295 373 3063
Fragments arranged by size:
3063 2295 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2902 2963 3794 5178 5446 1632
Size: 1270 61 831 1384 268 1917
Fragments arranged by size:
1917 1384 1270 831 268 61
AflII C'TTAA_G
Cuts at: 820 1017 2192 820
Size: 197 1175 4359
Fragments arranged by size:
4359 1175 197
AflIII A'CryG_T
Cuts at: 1203 4630 5607 1203
Size: 3427 977 1327
Fragments arranged by size:
3427 1327 977
AgeI A'CCGG_T
Cuts at: 2655 4562 2655
Size: 1907 3824
AhdI GACnn_n'nnGTC
Cuts at: 4460 4460
Size: 5731
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5731
AlwNI CAG_nnn'CTG
Cuts at: 3474 5046 3474
Size: 1572 4159
ApaLI G'TGCA_C
Cuts at: 2053 3788 4944 2053
Size: 1735 1156 2840
Fragments arranged by size:
2840 1735 1156
ApoI r'AATT_y
Cuts at: 1493 2244 2772 2923 3040 3266 3418 5527 1493
Size: 751 528 151 117 226 152 2109 1697
Fragments arranged by size:
2109 1697 751 528 226 152 151 117
AseI AT'TA_AT
Cuts at: 160 1032 2604 4285 160
Size: 872 1572 1681 1606
Fragments arranged by size:
1681 1606 1572 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
AvaI C'yCGr_G
Cuts at: 1870 1943 2796 3056 1870
Size: 73 853 260 4545
Fragments arranged by size:
4545 853 260 73
BamHI G'GATC_C
Cuts at: 2884 3061 3428 2884
Size: 177 367 5187
Fragments arranged by size:
5187 367 177
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3052 4507 618
Size: 325 217 627 1265 1455 1842
Fragments arranged by size:
1842 1455 1265 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3050 1391
Size: 1659 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2384 928
Size: 971 485 4275
Fragments arranged by size:
4275 971 485
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3922 3956 1903
Size: 34 50 34 1901 34 3678
Fragments arranged by size:
3678 1901 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3623 4833 3623
Size: 1210 4521
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5731
BfrBI ATG'CAT
Cuts at: 2876 2876
Size: 5731
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4342 136
Size: 107 122 71 631 605 2670 1525
Fragments arranged by size:
2670 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5726 5726
Size: 5731
BlpI GC'TnA_GC
Cuts at: 3148 3148
Size: 5731
Bme1580I G_kGCm'C
Cuts at: 1790 2057 2951 3792 4948 1790
Size: 267 894 841 1156 2573
Fragments arranged by size:
2573 1156 894 841 267
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2273 3462 4420
Size: 416 53 83 399 24 856 1189 958
Cuts at: 4420 442
Size: 1753
Fragments arranged by size:
1753 1189 958 856 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4391 1239
Size: 3152 2579
Bpu10I CC'TnA_GC
Cuts at: 2786 2786
Size: 5731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3856 4721 5019 3856
Size: 865 298 4568
Fragments arranged by size:
4568 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4394 882
Size: 617 2895 2219
Fragments arranged by size:
2895 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2665 3522 493
Size: 711 1461 857 2702
Fragments arranged by size:
2702 1461 857 711
BsaBI GATnn'nnATC
Cuts at: 2360 5725 2360
Size: 3365 2366
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3538 3920 4555 275
Size: 53 83 186 936 2005 382 635 1451
Fragments arranged by size:
2005 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2163 2655 4164 4562 4836 4983 5394 2163
Size: 492 1509 398 274 147 411 2500
Fragments arranged by size:
2500 1509 492 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5506
Fragments arranged by size:
5506 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5731
BseYI C'CCAG_C
Cuts at: 4934 5430 4934
Size: 496 5235
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2797 1163
Size: 573 1061 4097
Fragments arranged by size:
4097 1061 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2368 3181 3942 4970 1540
Size: 275 186 367 813 761 1028 2301
Fragments arranged by size:
2301 1028 813 761 367 275 186
BsiHKAI G_wGCw'C
Cuts at: 2057 2852 3792 3877 4948 2057
Size: 795 940 85 1071 2840
Fragments arranged by size:
2840 1071 940 795 85
BsmI GAATG_Cn'
Cuts at: 2453 3242 3335 2453
Size: 789 93 4849
Fragments arranged by size:
4849 789 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2852 2951 3050 3792 3877 4948
Size: 399 267 795 99 99 742 85 1071
Cuts at: 4948 1391
Size: 2174
Fragments arranged by size:
2174 1071 795 742 399 267 99 99
85
BspEI T'CCGG_A
Cuts at: 2163 5394 2163
Size: 3231 2500
BspHI T'CATG_A
Cuts at: 2220 3620 5350 2220
Size: 1400 1730 2601
Fragments arranged by size:
2601 1730 1400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3086 844
Size: 2242 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3618 1394
Size: 429 1795 3507
Fragments arranged by size:
3507 1795 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2727 4226 4400 65
Size: 1183 1479 1499 174 1396
Fragments arranged by size:
1499 1479 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2655 4375 4562 1700
Size: 110 845 1720 187 2869
Fragments arranged by size:
2869 1720 845 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5731
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5731
BssSI C'ACGA_G
Cuts at: 3791 4803 3791
Size: 1012 4719
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5731
BstBI TT'CG_AA
Cuts at: 4535 4535
Size: 5731
BstEII G'GTnAC_C
Cuts at: 1184 3005 1184
Size: 1821 3910
BstYI r'GATC_y
Cuts at: 1972 2884 3061 3428 3813 3830 5282 5368 5380
Size: 912 177 367 385 17 1452 86 12
Cuts at: 5380 5726 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 912 385 367 346 177 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5179
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2156 487
Size: 683 56 930 4062
Fragments arranged by size:
4062 930 683 56
BtsI GCAGTG_nn'
Cuts at: 2090 2814 3247 4041 4061 2090
Size: 724 433 794 20 3760
Fragments arranged by size:
3760 794 724 433 20
ClaI AT'CG_AT
Cuts at: 1554 2364 1554
Size: 810 4921
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5696
DraI TTT'AAA
Cuts at: 2395 3035 2395
Size: 640 5091
DraIII CAC_nnn'GTG
Cuts at: 2856 2856
Size: 5731
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4738 809
Size: 507 3422 1802
Fragments arranged by size:
3422 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3178 4067 5696
Size: 54 1425 50 275 18 1348 889 1629
Cuts at: 5696 8
Size: 43
Fragments arranged by size:
1629 1425 1348 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3178 1537
Size: 275 1366 4090
Fragments arranged by size:
4090 1366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 3012 3661 1353
Size: 737 922 649 3423
Fragments arranged by size:
3423 922 737 649
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4318 4692 4838 387
Size: 1064 138 2729 374 146 1280
Fragments arranged by size:
2729 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2902 2963 3794 4391 5178 5446 1239
Size: 393 1270 61 831 597 787 268 1524
Fragments arranged by size:
1524 1270 831 787 597 393 268 61
EcoO109I rG'GnC_Cy
Cuts at: 2080 2080
Size: 5731
EcoRI G'AATT_C
Cuts at: 2772 3040 3418 2772
Size: 268 378 5085
Fragments arranged by size:
5085 378 268
FspI TGC'GCA
Cuts at: 4237 4237
Size: 5731
HaeII r_GCGC'y
Cuts at: 1678 4878 1678
Size: 3200 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4372 4404 4446 4478 1373
Size: 32 135 32 2800 32 42 32 2626
Fragments arranged by size:
2800 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2017 2862 3075 3321 669
Size: 988 281 79 845 213 246 3079
Fragments arranged by size:
3079 988 845 281 246 213 79
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5731
HpaI GTT'AAC
Cuts at: 2862 3321 2862
Size: 459 5272
KpnI G_GTAC'C
Cuts at: 3056 3056
Size: 5731
MfeI C'AATT_G
Cuts at: 3330 3330
Size: 5731
MluI A'CGCG_T
Cuts at: 5607 5607
Size: 5731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2098 2427 4845 5029 1305
Size: 793 329 2418 184 2007
Fragments arranged by size:
2418 2007 793 329 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3909
Fragments arranged by size:
3909 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3689 4048 4207 518
Size: 648 2523 359 159 2042
Fragments arranged by size:
2523 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3147 3824 4972 5217 1606
Size: 1541 677 1148 245 2120
Fragments arranged by size:
2120 1541 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5179
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5731
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5731
NotI GC'GGCC_GC
Cuts at: 3178 3178
Size: 5731
NsiI A_TGCA'T
Cuts at: 2878 2878
Size: 5731
NspI r_CATG'y
Cuts at: 1132 1229 2880 3089 4634 1132
Size: 97 1651 209 1545 2229
Fragments arranged by size:
2229 1651 1545 209 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5731
PciI A'CATG_T
Cuts at: 4630 4630
Size: 5731
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5731
PmeI GTTT'AAAC
Cuts at: 3035 3035
Size: 5731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 5313 5345 3800
Size: 32 1481 32 4186
Fragments arranged by size:
4186 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2080 2080
Size: 5731
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5731
PsiI TTA'TAA
Cuts at: 3301 3301
Size: 5731
PstI C_TGCA'G
Cuts at: 830 3083 830
Size: 2253 3478
PvuI CG_AT'CG
Cuts at: 2001 2368 2001
Size: 367 5364
SalI G'TCGA_C
Cuts at: 1936 2015 3073 1936
Size: 79 1058 4594
Fragments arranged by size:
4594 1058 79
SbfI CC_TGCA'GG
Cuts at: 3083 3083
Size: 5731
ScaI AGT'ACT
Cuts at: 3979 5691 3979
Size: 1712 4019
SfcI C'TryA_G
Cuts at: 826 1045 3079 4214 4895 5086 826
Size: 219 2034 1135 681 191 1471
Fragments arranged by size:
2034 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5731
SmaI CCC'GGG
Cuts at: 1872 2798 3058 1872
Size: 926 260 4545
Fragments arranged by size:
4545 926 260
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2192 3835 4736 4998 820
Size: 197 926 249 1643 901 262 1553
Fragments arranged by size:
1643 1553 926 901 262 249 197
SnaBI TAC'GTA
Cuts at: 493 2665 3522 493
Size: 2172 857 2702
Fragments arranged by size:
2702 2172 857
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5731
SphI G_CATG'C
Cuts at: 1132 2880 3089 1132
Size: 1748 209 3774
Fragments arranged by size:
3774 1748 209
SspI AAT'ATT
Cuts at: 5 52 3655 5
Size: 47 3603 2081
Fragments arranged by size:
3603 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5731
StyI C'CwwG_G
Cuts at: 513 1065 3170 513
Size: 552 2105 3074
Fragments arranged by size:
3074 2105 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2090 2540 3758 3775 3928 4113 4558
Size: 1255 510 450 1218 17 153 185 445
Cuts at: 4558 325
Size: 1498
Fragments arranged by size:
1498 1255 1218 510 450 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3977 5689 96
Size: 274 80 33 51 1443 2000 1712 138
Fragments arranged by size:
2000 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5691 5723 5691
Size: 32 5699
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5731
XbaI T'CTAG_A
Cuts at: 3067 3067
Size: 5731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2416 2416
Size: 5731
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5731
XmaI C'CCGG_G
Cuts at: 1870 2796 3056 1870
Size: 926 260 4545
Fragments arranged by size:
4545 926 260
XmnI GAAnn'nnTTC
Cuts at: 3860 3860
Size: 5731
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3539 276
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI