Restriction map for
pFN21AB9829
(Circular) (Six-base) MAPSORT of: pFN21AB9829.seq Check: 4887 from: 1 to: 5725
pFN21AB9829 5725 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2329 2329
Size: 5725
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3535 278
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2620 3068 1937
Size: 683 448 4594
Fragments arranged by size:
4594 683 448
Acc65I G'GTAC_C
Cuts at: 3046 3046
Size: 5725
AclI AA'CG_TT
Cuts at: 1563 3852 4225 1563
Size: 2289 373 3063
Fragments arranged by size:
3063 2289 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3788 5172 5440 1632
Size: 2156 1384 268 1917
Fragments arranged by size:
2156 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5528
AflIII A'CryG_T
Cuts at: 1203 2282 4624 5601 1203
Size: 1079 2342 977 1327
Fragments arranged by size:
2342 1327 1079 977
AgeI A'CCGG_T
Cuts at: 4556 4556
Size: 5725
AhdI GACnn_n'nnGTC
Cuts at: 4454 4454
Size: 5725
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2162 2285 2361 1184
Size: 978 123 76 4548
Fragments arranged by size:
4548 978 123 76
AlwNI CAG_nnn'CTG
Cuts at: 3468 5040 3468
Size: 1572 4153
ApaI G_GGCC'C
Cuts at: 2177 2785 2177
Size: 608 5117
ApaLI G'TGCA_C
Cuts at: 3782 4938 3782
Size: 1156 4569
ApoI r'AATT_y
Cuts at: 1493 3034 3260 3412 5521 1493
Size: 1541 226 152 2109 1697
Fragments arranged by size:
2109 1697 1541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4279 160
Size: 872 3247 1606
Fragments arranged by size:
3247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
AvaI C'yCGr_G
Cuts at: 1870 1943 2153 3050 1870
Size: 73 210 897 4545
Fragments arranged by size:
4545 897 210 73
BamHI G'GATC_C
Cuts at: 3055 3422 3055
Size: 367 5358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2393 2708 2746 3046 4501
Size: 325 217 627 606 315 38 300 1455
Cuts at: 4501 618
Size: 1842
Fragments arranged by size:
1842 1455 627 606 325 315 300 217
38
BanII G_rGCy'C
Cuts at: 1391 2077 2087 2177 2785 3044 1391
Size: 686 10 90 608 259 4072
Fragments arranged by size:
4072 686 608 259 90 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2165 2285 2437 2533 928
Size: 971 266 120 152 96 4120
Fragments arranged by size:
4120 971 266 152 120 96
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3916 3950 1903
Size: 34 50 34 1895 34 3678
Fragments arranged by size:
3678 1895 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3617 4827 3617
Size: 1210 4515
BclI T'GATC_A
Cuts at: 1548 2049 1548
Size: 501 5224
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2014 2433 4336
Size: 107 122 71 631 605 342 419 1903
Cuts at: 4336 136
Size: 1525
Fragments arranged by size:
1903 1525 631 605 419 342 122 107
71
BglII A'GATC_T
Cuts at: 5720 5720
Size: 5725
BlpI GC'TnA_GC
Cuts at: 3142 3142
Size: 5725
Bme1580I G_kGCm'C
Cuts at: 1790 2177 2398 2713 2751 2785 3786 4942 1790
Size: 387 221 315 38 34 1001 1156 2573
Fragments arranged by size:
2573 1156 1001 387 315 221 38 34
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5725
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3456 4414 442
Size: 416 53 83 399 24 2039 958 1753
Fragments arranged by size:
2039 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5725
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 5725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2747 4385 1239
Size: 1508 1638 2579
Fragments arranged by size:
2579 1638 1508
Bpu10I CC'TnA_GC
Cuts at: 2121 2349 2526 2121
Size: 228 177 5320
Fragments arranged by size:
5320 228 177
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3850 4715 5013 3850
Size: 865 298 4562
Fragments arranged by size:
4562 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4388 882
Size: 617 2889 2219
Fragments arranged by size:
2889 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2285 3516 493
Size: 711 1081 1231 2702
Fragments arranged by size:
2702 1231 1081 711
BsaBI GATnn'nnATC
Cuts at: 5719 5719
Size: 5725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3532 3914 4549 275
Size: 53 83 186 936 1999 382 635 1451
Fragments arranged by size:
1999 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4158 4556 4830 4977 5388 4158
Size: 398 274 147 411 4495
Fragments arranged by size:
4495 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2984 3014 1178
Size: 30 165 30 1581 30 3889
Fragments arranged by size:
3889 1581 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2579 1186
Size: 1393 4332
BseYI C'CCAG_C
Cuts at: 2063 2125 2428 2459 4928 5424 2063
Size: 62 303 31 2469 496 2364
Fragments arranged by size:
2469 2364 496 303 62 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5152
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3175 3936 4964 1540
Size: 275 186 1174 761 1028 2301
Fragments arranged by size:
2301 1174 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2370 2470 2979 3786 3871 4942 2370
Size: 100 509 807 85 1071 3153
Fragments arranged by size:
3153 1071 807 509 100 85
BsmI GAATG_Cn'
Cuts at: 2686 3236 3329 2686
Size: 550 93 5082
Fragments arranged by size:
5082 550 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2087 2177 2370 2398 2470 2713
Size: 399 287 10 90 193 28 72 243
Cuts at: 2713 2751 2785 2979 3044 3786 3871 4942 1391
Size: 38 34 194 65 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 287 243 194 193
90 85 72 65 38 34 28 10
BspEI T'CCGG_A
Cuts at: 5388 5388
Size: 5725
BspHI T'CATG_A
Cuts at: 3614 5344 3614
Size: 1730 3995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2329 3080 844
Size: 1485 751 3489
Fragments arranged by size:
3489 1485 751
BsrBI CCG'CTC
Cuts at: 1394 1823 3612 1394
Size: 429 1789 3507
Fragments arranged by size:
3507 1789 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2669 4220 4394 65
Size: 1183 1421 1551 174 1396
Fragments arranged by size:
1551 1421 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2334 4369 4556 1700
Size: 110 524 2035 187 2869
Fragments arranged by size:
2869 2035 524 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5725
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5725
BssSI C'ACGA_G
Cuts at: 3785 4797 3785
Size: 1012 4713
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2671 794
Size: 1877 3848
BstBI TT'CG_AA
Cuts at: 4529 4529
Size: 5725
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5725
BstXI CCAn_nnnn'nTGG
Cuts at: 2641 2641
Size: 5725
BstYI r'GATC_y
Cuts at: 1972 3055 3422 3807 3824 5276 5362 5374 5720
Size: 1083 367 385 17 1452 86 12 346
Cuts at: 5720 1972
Size: 1977
Fragments arranged by size:
1977 1452 1083 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2178 2178
Size: 5725
BtgI C'CryG_G
Cuts at: 513 1065 2055 513
Size: 552 990 4183
Fragments arranged by size:
4183 990 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4986
Fragments arranged by size:
4986 683 56
BtsI GCAGTG_nn'
Cuts at: 2221 3241 4035 4055 2221
Size: 1020 794 20 3891
Fragments arranged by size:
3891 1020 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5690
DraI TTT'AAA
Cuts at: 3029 3029
Size: 5725
DraIII CAC_nnn'GTG
Cuts at: 2474 2778 2474
Size: 304 5421
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4732 809
Size: 507 3416 1802
Fragments arranged by size:
3416 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2015 3172 4061
Size: 54 1425 50 275 18 185 1157 889
Cuts at: 4061 5690 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1157 889 275 185 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3172 1537
Size: 275 1360 4090
Fragments arranged by size:
4090 1360 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2940 3655 1353
Size: 1587 715 3423
Fragments arranged by size:
3423 1587 715
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4312 4686 4832 387
Size: 1064 138 2723 374 146 1280
Fragments arranged by size:
2723 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2747 3788 4385 5172 5440 1239
Size: 393 1115 1041 597 787 268 1524
Fragments arranged by size:
1524 1115 1041 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2174 2782 2915 2950 2174
Size: 608 133 35 4949
Fragments arranged by size:
4949 608 133 35
EcoRI G'AATT_C
Cuts at: 3034 3412 3034
Size: 378 5347
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2969 2969
Size: 5725
FspI TGC'GCA
Cuts at: 4231 4231
Size: 5725
HaeII r_GCGC'y
Cuts at: 1678 2307 2490 2992 4872 1678
Size: 629 183 502 1880 2531
Fragments arranged by size:
2531 1880 629 502 183
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4366 4398 4440 4472 1373
Size: 32 135 32 2794 32 42 32 2626
Fragments arranged by size:
2794 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2621 3069 3315 669
Size: 988 281 683 448 246 3079
Fragments arranged by size:
3079 988 683 448 281 246
HindIII A'AGCT_T
Cuts at: 748 2636 748
Size: 1888 3837
HpaI GTT'AAC
Cuts at: 3315 3315
Size: 5725
KpnI G_GTAC'C
Cuts at: 3050 3050
Size: 5725
MfeI C'AATT_G
Cuts at: 3324 3324
Size: 5725
MluI A'CGCG_T
Cuts at: 5601 5601
Size: 5725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2892 4839 5023 1305
Size: 1587 1947 184 2007
Fragments arranged by size:
2007 1947 1587 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2017 10
Size: 54 1425 343 185 3718
Fragments arranged by size:
3718 1425 343 185 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3683 4042 4201 518
Size: 648 2517 359 159 2042
Fragments arranged by size:
2517 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2063 2269 2404 2432 2463 3141 3818 4966
Size: 457 206 135 28 31 678 677 1148
Cuts at: 4966 5211 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 678 677 457 245 206 135
31 28
NcoI C'CATG_G
Cuts at: 513 1065 2055 513
Size: 552 990 4183
Fragments arranged by size:
4183 990 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5725
NotI GC'GGCC_GC
Cuts at: 3172 3172
Size: 5725
NspI r_CATG'y
Cuts at: 1132 1229 2887 2912 3083 4628 1132
Size: 97 1658 25 171 1545 2229
Fragments arranged by size:
2229 1658 1545 171 97 25
PasI CC'CwG_GG
Cuts at: 1348 2569 2809 1348
Size: 1221 240 4264
Fragments arranged by size:
4264 1221 240
PciI A'CATG_T
Cuts at: 4624 4624
Size: 5725
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2778 1108
Size: 1670 4055
PfoI T'CCnGG_A
Cuts at: 2982 2982
Size: 5725
PmeI GTTT'AAAC
Cuts at: 3029 3029
Size: 5725
PmlI CAC'GTG
Cuts at: 2285 2285
Size: 5725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 5307 5339 3794
Size: 32 1481 32 4180
Fragments arranged by size:
4180 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5725
PsiI TTA'TAA
Cuts at: 3295 3295
Size: 5725
PspOMI G'GGCC_C
Cuts at: 2173 2781 2173
Size: 608 5117
PstI C_TGCA'G
Cuts at: 830 2551 2563 3077 830
Size: 1721 12 514 3478
Fragments arranged by size:
3478 1721 514 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5725
PvuII CAG'CTG
Cuts at: 2063 2404 2432 2063
Size: 341 28 5356
Fragments arranged by size:
5356 341 28
SalI G'TCGA_C
Cuts at: 1936 2619 3067 1936
Size: 683 448 4594
Fragments arranged by size:
4594 683 448
SbfI CC_TGCA'GG
Cuts at: 2551 3077 2551
Size: 526 5199
ScaI AGT'ACT
Cuts at: 3973 5685 3973
Size: 1712 4013
SexAI A'CCwGG_T
Cuts at: 2389 2871 2389
Size: 482 5243
SfcI C'TryA_G
Cuts at: 826 1045 2547 2559 2598 2692 3073 4208 4889
Size: 219 1502 12 39 94 381 1135 681
Cuts at: 4889 5080 826
Size: 191 1471
Fragments arranged by size:
1502 1471 1135 681 381 219 191 94
39 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5725
SmaI CCC'GGG
Cuts at: 1872 3052 1872
Size: 1180 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2153 3829 4730 4992 820
Size: 197 926 210 1676 901 262 1553
Fragments arranged by size:
1676 1553 926 901 262 210 197
SnaBI TAC'GTA
Cuts at: 493 3516 493
Size: 3023 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5725
SphI G_CATG'C
Cuts at: 1132 2912 3083 1132
Size: 1780 171 3774
Fragments arranged by size:
3774 1780 171
SspI AAT'ATT
Cuts at: 5 52 3649 5
Size: 47 3597 2081
Fragments arranged by size:
3597 2081 47
StuI AGG'CCT
Cuts at: 1517 2524 1517
Size: 1007 4718
StyI C'CwwG_G
Cuts at: 513 1065 2055 3164 513
Size: 552 990 1109 3074
Fragments arranged by size:
3074 1109 990 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2696 2809 2837 3752 3769 3922 4107
Size: 1255 1116 113 28 915 17 153 185
Cuts at: 4107 4552 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1116 915 445 185 153 113
28 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3971 5683 96
Size: 274 80 33 51 1443 1994 1712 138
Fragments arranged by size:
1994 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2873 2905 2984 3016 5685 5717 2873
Size: 32 79 32 2669 32 2881
Fragments arranged by size:
2881 2669 79 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2873 1316
Size: 1557 4168
XbaI T'CTAG_A
Cuts at: 3061 3061
Size: 5725
XhoI C'TCGA_G
Cuts at: 1943 2153 1943
Size: 210 5515
XmaI C'CCGG_G
Cuts at: 1870 3050 1870
Size: 1180 4545
XmnI GAAnn'nnTTC
Cuts at: 3854 3854
Size: 5725
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3533 276
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI XcmI