Restriction map for
pFN21AB9828
(Circular) (Six-base) MAPSORT of: pFN21AB9828.seq Check: 7403 from: 1 to: 5695
pFN21AB9828 5695 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2860 2860
Size: 5695
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3505 278
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3038 1937
Size: 1101 4594
Acc65I G'GTAC_C
Cuts at: 2229 3016 2229
Size: 787 4908
AclI AA'CG_TT
Cuts at: 1563 3822 4195 1563
Size: 2259 373 3063
Fragments arranged by size:
3063 2259 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2820 3758 5142 5410 1632
Size: 1188 938 1384 268 1917
Fragments arranged by size:
1917 1384 1188 938 268
AfeI AGC'GCT
Cuts at: 2324 2324
Size: 5695
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5498
AflIII A'CryG_T
Cuts at: 1203 2721 4594 5571 1203
Size: 1518 1873 977 1327
Fragments arranged by size:
1873 1518 1327 977
AgeI A'CCGG_T
Cuts at: 4526 4526
Size: 5695
AhdI GACnn_n'nnGTC
Cuts at: 4424 4424
Size: 5695
AleI CACnn'nnGTG
Cuts at: 1166 2432 1166
Size: 1266 4429
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5695
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2959 2991 2959
Size: 32 5663
AlwNI CAG_nnn'CTG
Cuts at: 2875 3438 5010 2875
Size: 563 1572 3560
Fragments arranged by size:
3560 1572 563
ApaI G_GGCC'C
Cuts at: 2647 2647
Size: 5695
ApaLI G'TGCA_C
Cuts at: 2135 3752 4908 2135
Size: 1617 1156 2922
Fragments arranged by size:
2922 1617 1156
ApoI r'AATT_y
Cuts at: 1493 3004 3230 3382 5491 1493
Size: 1511 226 152 2109 1697
Fragments arranged by size:
2109 1697 1511 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4249 160
Size: 872 3217 1606
Fragments arranged by size:
3217 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
AvaI C'yCGr_G
Cuts at: 1870 1943 3020 1870
Size: 73 1077 4545
Fragments arranged by size:
4545 1077 73
BamHI G'GATC_C
Cuts at: 2109 3025 3392 2109
Size: 916 367 4412
Fragments arranged by size:
4412 916 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2229 2655 3016 4471 618
Size: 325 217 627 442 426 361 1455 1842
Fragments arranged by size:
1842 1455 627 442 426 361 325 217
BanII G_rGCy'C
Cuts at: 1391 2027 2647 2891 3014 1391
Size: 636 620 244 123 4072
Fragments arranged by size:
4072 636 620 244 123
BbeI G_GCGC'C
Cuts at: 2659 2659
Size: 5695
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4724
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2299 2333 3886 3920 1903
Size: 34 50 34 278 34 1553 34 3678
Fragments arranged by size:
3678 1553 278 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3587 4797 3587
Size: 1210 4485
BclI T'GATC_A
Cuts at: 1548 2436 1548
Size: 888 4807
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2093 2542 4306
Size: 107 122 71 631 605 421 449 1764
Cuts at: 4306 136
Size: 1525
Fragments arranged by size:
1764 1525 631 605 449 421 122 107
71
BglII A'GATC_T
Cuts at: 5690 5690
Size: 5695
BlpI GC'TnA_GC
Cuts at: 2150 3112 2150
Size: 962 4733
Bme1580I G_kGCm'C
Cuts at: 1790 2139 2647 3756 4912 1790
Size: 349 508 1109 1156 2573
Fragments arranged by size:
2573 1156 1109 508 349
BmgBI CAC'GTC
Cuts at: 1318 2527 1318
Size: 1209 4486
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3426 4384 442
Size: 416 53 83 399 24 2009 958 1753
Fragments arranged by size:
2009 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2567 4355 1239
Size: 1328 1788 2579
Fragments arranged by size:
2579 1788 1328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3820 4685 4983 3820
Size: 865 298 4532
Fragments arranged by size:
4532 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4358 882
Size: 617 2859 2219
Fragments arranged by size:
2859 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2295 3486 493
Size: 711 1091 1191 2702
Fragments arranged by size:
2702 1191 1091 711
BsaBI GATnn'nnATC
Cuts at: 5689 5689
Size: 5695
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2656 3502 3884 4519
Size: 53 83 186 936 1123 846 382 635
Cuts at: 4519 275
Size: 1451
Fragments arranged by size:
1451 1123 936 846 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4128 4526 4800 4947 5358 4128
Size: 398 274 147 411 4465
Fragments arranged by size:
4465 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2345 2375 1178
Size: 30 165 30 942 30 4498
Fragments arranged by size:
4498 942 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 2881 1186
Size: 976 719 4000
Fragments arranged by size:
4000 976 719
BseYI C'CCAG_C
Cuts at: 2121 2628 4898 5394 2121
Size: 507 2270 496 2422
Fragments arranged by size:
2422 2270 507 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2112 2369 2410 1163
Size: 573 376 257 41 4448
Fragments arranged by size:
4448 573 376 257 41
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3145 3906 4934 1540
Size: 275 186 1144 761 1028 2301
Fragments arranged by size:
2301 1144 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2027 2139 2565 3756 3841 4912 2027
Size: 112 426 1191 85 1071 2810
Fragments arranged by size:
2810 1191 1071 426 112 85
BsmI GAATG_Cn'
Cuts at: 3206 3299 3206
Size: 93 5602
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2139 2565 2647 2891 3014 3756
Size: 399 237 112 426 82 244 123 742
Cuts at: 3756 3841 4912 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 426 399 244 237 123
112 85 82
BspEI T'CCGG_A
Cuts at: 5358 5358
Size: 5695
BspHI T'CATG_A
Cuts at: 3584 5314 3584
Size: 1730 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2241 2458 2501 2860 3050 844
Size: 1397 217 43 359 190 3489
Fragments arranged by size:
3489 1397 359 217 190 43
BsrBI CCG'CTC
Cuts at: 1394 1823 3582 1394
Size: 429 1759 3507
Fragments arranged by size:
3507 1759 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2488 4190 4364 65
Size: 1183 1240 1702 174 1396
Fragments arranged by size:
1702 1396 1240 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2367 4339 4526 1700
Size: 110 557 1972 187 2869
Fragments arranged by size:
2869 1972 557 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5695
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5695
BssSI C'ACGA_G
Cuts at: 2257 3755 4767 2257
Size: 1498 1012 3185
Fragments arranged by size:
3185 1498 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2286 2875 794
Size: 1492 589 3614
Fragments arranged by size:
3614 1492 589
BstBI TT'CG_AA
Cuts at: 4499 4499
Size: 5695
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5695
BstXI CCAn_nnnn'nTGG
Cuts at: 2083 2083
Size: 5695
BstYI r'GATC_y
Cuts at: 1972 2109 3025 3392 3777 3794 5246 5332 5344
Size: 137 916 367 385 17 1452 86 12
Cuts at: 5344 5690 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 916 385 367 346 137 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2577 2664 2778 513
Size: 552 1512 87 114 3430
Fragments arranged by size:
3430 1512 552 114 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4956
Fragments arranged by size:
4956 683 56
BtsI GCAGTG_nn'
Cuts at: 2422 3211 4005 4025 2422
Size: 789 794 20 4092
Fragments arranged by size:
4092 794 789 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2760 2795 529
Size: 35 2196 35 3429
Fragments arranged by size:
3429 2196 35 35
DraI TTT'AAA
Cuts at: 2999 2999
Size: 5695
DraIII CAC_nnn'GTG
Cuts at: 2734 2734
Size: 5695
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4702 809
Size: 507 3386 1802
Fragments arranged by size:
3386 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2163 3142 4031
Size: 54 1425 50 275 18 333 979 889
Cuts at: 4031 5660 8
Size: 1629 43
Fragments arranged by size:
1629 1425 979 889 333 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3142 1537
Size: 275 1330 4090
Fragments arranged by size:
4090 1330 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2015 2570 2825 2836 3625 1353
Size: 662 555 255 11 789 3423
Fragments arranged by size:
3423 789 662 555 255 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2102 2122 4282 4656 4802 387
Size: 1064 138 513 20 2160 374 146 1280
Fragments arranged by size:
2160 1280 1064 513 374 146 138 20
EcoICRI GAG'CTC
Cuts at: 2025 2025
Size: 5695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2567 2820 3758 4355 5142 5410 1239
Size: 393 935 253 938 597 787 268 1524
Fragments arranged by size:
1524 938 935 787 597 393 268 253
EcoNI CCTnn'n_nnAGG
Cuts at: 2237 2237
Size: 5695
EcoO109I rG'GnC_Cy
Cuts at: 2643 2643
Size: 5695
EcoRI G'AATT_C
Cuts at: 3004 3382 3004
Size: 378 5317
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2044 2044
Size: 5695
FspI TGC'GCA
Cuts at: 2846 4201 2846
Size: 1355 4340
HaeII r_GCGC'y
Cuts at: 1678 2326 2659 4842 1678
Size: 648 333 2183 2531
Fragments arranged by size:
2531 2183 648 333
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2198 2230 2377 2409 4336
Size: 32 135 32 626 32 147 32 1927
Cuts at: 4336 4368 4410 4442 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1927 626 147 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3039 3285 669
Size: 988 281 1101 246 3079
Fragments arranged by size:
3079 1101 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5695
HpaI GTT'AAC
Cuts at: 3285 3285
Size: 5695
KasI G'GCGC_C
Cuts at: 2655 2655
Size: 5695
KpnI G_GTAC'C
Cuts at: 2233 3020 2233
Size: 787 4908
MfeI C'AATT_G
Cuts at: 3294 3294
Size: 5695
MluI A'CGCG_T
Cuts at: 5571 5571
Size: 5695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2502 4809 4993 1305
Size: 1197 2307 184 2007
Fragments arranged by size:
2307 2007 1197 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3873
Fragments arranged by size:
3873 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2432 2576 3653 4012 4171 518
Size: 648 1266 144 1077 359 159 2042
Fragments arranged by size:
2042 1266 1077 648 359 159 144
MspA1I CmG'CkG
Cuts at: 1606 2226 3111 3788 4936 5181 1606
Size: 620 885 677 1148 245 2120
Fragments arranged by size:
2120 1148 885 677 620 245
NaeI GCC'GGC
Cuts at: 2369 2369
Size: 5695
NarI GG'CG_CC
Cuts at: 2656 2656
Size: 5695
NcoI C'CATG_G
Cuts at: 513 1065 2577 513
Size: 552 1512 3631
Fragments arranged by size:
3631 1512 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5695
NgoMIV G'CCGG_C
Cuts at: 2367 2367
Size: 5695
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5695
NotI GC'GGCC_GC
Cuts at: 3142 3142
Size: 5695
NspI r_CATG'y
Cuts at: 1132 1229 3053 4598 1132
Size: 97 1824 1545 2229
Fragments arranged by size:
2229 1824 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5695
PciI A'CATG_T
Cuts at: 4594 4594
Size: 5695
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2734 1108
Size: 1626 4069
PmeI GTTT'AAAC
Cuts at: 2999 2999
Size: 5695
PmlI CAC'GTG
Cuts at: 2295 2295
Size: 5695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 5277 5309 3764
Size: 32 1481 32 4150
Fragments arranged by size:
4150 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5695
PsiI TTA'TAA
Cuts at: 3265 3265
Size: 5695
PspOMI G'GGCC_C
Cuts at: 2643 2643
Size: 5695
PstI C_TGCA'G
Cuts at: 830 2291 2871 3047 830
Size: 1461 580 176 3478
Fragments arranged by size:
3478 1461 580 176
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5695
SacI G_AGCT'C
Cuts at: 2027 2027
Size: 5695
SalI G'TCGA_C
Cuts at: 1936 3037 1936
Size: 1101 4594
SapI GCTCTTCn'nnn_
Cuts at: 2015 2570 2825 2015
Size: 555 255 4885
Fragments arranged by size:
4885 555 255
SbfI CC_TGCA'GG
Cuts at: 3047 3047
Size: 5695
ScaI AGT'ACT
Cuts at: 3943 5655 3943
Size: 1712 3983
SexAI A'CCwGG_T
Cuts at: 2730 2730
Size: 5695
SfcI C'TryA_G
Cuts at: 826 1045 2272 2287 2867 2988 3043 4178 4859
Size: 219 1227 15 580 121 55 1135 681
Cuts at: 4859 5050 826
Size: 191 1471
Fragments arranged by size:
1471 1227 1135 681 580 219 191 121
55 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 2093 2093
Size: 5695
SfoI GGC'GCC
Cuts at: 2657 2657
Size: 5695
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5695
SmaI CCC'GGG
Cuts at: 1872 3022 1872
Size: 1150 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3799 4700 4962 820
Size: 197 926 1856 901 262 1553
Fragments arranged by size:
1856 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3486 493
Size: 2993 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5695
SphI G_CATG'C
Cuts at: 1132 3053 1132
Size: 1921 3774
SspI AAT'ATT
Cuts at: 5 52 3619 5
Size: 47 3567 2081
Fragments arranged by size:
3567 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5695
StyI C'CwwG_G
Cuts at: 513 1065 2238 2577 3134 513
Size: 552 1173 339 557 3074
Fragments arranged by size:
3074 1173 557 552 339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3722 3739 3892 4077 4522 325
Size: 1255 2142 17 153 185 445 1498
Fragments arranged by size:
2142 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2352 3941 5653
Size: 274 80 33 51 1443 375 1589 1712
Cuts at: 5653 96
Size: 138
Fragments arranged by size:
1712 1589 1443 375 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5655 5687 5655
Size: 32 5663
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5695
XbaI T'CTAG_A
Cuts at: 3031 3031
Size: 5695
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5695
XmaI C'CCGG_G
Cuts at: 1870 3020 1870
Size: 1150 4545
XmnI GAAnn'nnTTC
Cuts at: 3824 3824
Size: 5695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3503 276
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI
PacI PfoI PpuMI PspXI PsrI PvuII RsrII SacII
SanDI SrfI SwaI XcmI