Restriction map for
pFN21AB9827
(Circular) (Six-base) MAPSORT of: pFN21AB9827.seq Check: 5118 from: 1 to: 5680
pFN21AB9827 5680 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2125 2125
Size: 5680
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 1937
Size: 1086 4594
Acc65I G'GTAC_C
Cuts at: 3001 3001
Size: 5680
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2117 2204 2554 2663 2785 3743 5127 5395
Size: 485 87 350 109 122 958 1384 268
Cuts at: 5395 1632
Size: 1917
Fragments arranged by size:
1917 1384 958 485 350 268 122 109
87
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 4579 5556 1203
Size: 3376 977 1327
Fragments arranged by size:
3376 1327 977
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 4409 4409
Size: 5680
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2645 2897 1184
Size: 1461 252 3967
Fragments arranged by size:
3967 1461 252
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2132 2164 2132
Size: 32 5648
AlwNI CAG_nnn'CTG
Cuts at: 2284 2299 2577 2641 2662 2931 3423 4995 2284
Size: 15 278 64 21 269 492 1572 2969
Fragments arranged by size:
2969 1572 492 278 269 64 21 15
ApaLI G'TGCA_C
Cuts at: 2813 3737 4893 2813
Size: 924 1156 3600
Fragments arranged by size:
3600 1156 924
ApoI r'AATT_y
Cuts at: 1493 2989 3215 3367 5476 1493
Size: 1496 226 152 2109 1697
Fragments arranged by size:
2109 1697 1496 226 152
AscI GG'CGCG_CC
Cuts at: 2416 2416
Size: 5680
AseI AT'TA_AT
Cuts at: 160 1032 4234 160
Size: 872 3202 1606
Fragments arranged by size:
3202 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 2587 2948 3005 1870
Size: 73 644 361 57 4545
Fragments arranged by size:
4545 644 361 73 57
BamHI G'GATC_C
Cuts at: 3010 3377 3010
Size: 367 5313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 3001 4456 618
Size: 325 217 627 234 980 1455 1842
Fragments arranged by size:
1842 1455 980 627 325 234 217
BanII G_rGCy'C
Cuts at: 1391 2999 1391
Size: 1608 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4709
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3871 3905 1903
Size: 34 1934 34 3678
Fragments arranged by size:
3678 1934 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3572 4782 3572
Size: 1210 4470
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5680
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2174 2402 4291
Size: 107 122 71 631 605 502 228 1889
Cuts at: 4291 136
Size: 1525
Fragments arranged by size:
1889 1525 631 605 502 228 122 107
71
BglII A'GATC_T
Cuts at: 2352 5675 2352
Size: 3323 2357
BlpI GC'TnA_GC
Cuts at: 2834 3097 2834
Size: 263 5417
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2817 3741 4897 1790
Size: 234 793 924 1156 2573
Fragments arranged by size:
2573 1156 924 793 234
BmgBI CAC'GTC
Cuts at: 1318 2446 2824 1318
Size: 1128 378 4174
Fragments arranged by size:
4174 1128 378
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2587 3411 4369
Size: 416 53 83 399 24 1170 824 958
Cuts at: 4369 442
Size: 1753
Fragments arranged by size:
1753 1170 958 824 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2704 2704
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2306 2699 4340 1239
Size: 1067 393 1641 2579
Fragments arranged by size:
2579 1641 1067 393
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2042 2111 3805 4670 4968 2042
Size: 69 1694 865 298 2754
Fragments arranged by size:
2754 1694 865 298 69
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2111 4343 882
Size: 617 612 2232 2219
Fragments arranged by size:
2232 2219 617 612
BsaAI yAC'GTr
Cuts at: 493 1204 3471 493
Size: 711 2267 2702
Fragments arranged by size:
2702 2267 711
BsaBI GATnn'nnATC
Cuts at: 5674 5674
Size: 5680
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2166 2841 3487 3869
Size: 53 83 186 936 633 675 646 382
Cuts at: 3869 4504 275
Size: 635 1451
Fragments arranged by size:
1451 936 675 646 635 633 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4113 4511 4785 4932 5343 4113
Size: 398 274 147 411 4450
Fragments arranged by size:
4450 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5455
Fragments arranged by size:
5455 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2129 2303 2495 2498 2501 2519 2567
Size: 859 84 174 192 3 3 18 48
Cuts at: 2567 1186
Size: 4299
Fragments arranged by size:
4299 859 192 174 84 48 18 3
3
BseYI C'CCAG_C
Cuts at: 2931 4883 5379 2931
Size: 1952 496 3232
Fragments arranged by size:
3232 1952 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2651 1163
Size: 573 915 4192
Fragments arranged by size:
4192 915 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2406 2476 3130 3891 4919 1540
Size: 275 186 405 70 654 761 1028 2301
Fragments arranged by size:
2301 1028 761 654 405 275 186 70
BsiHKAI G_wGCw'C
Cuts at: 2817 3741 3826 4897 2817
Size: 924 85 1071 3600
Fragments arranged by size:
3600 1071 924 85
BsmI GAATG_Cn'
Cuts at: 3191 3284 3191
Size: 93 5587
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2817 2999 3741 3826 4897 1391
Size: 399 234 793 182 742 85 1071 2174
Fragments arranged by size:
2174 1071 793 742 399 234 182 85
BspEI T'CCGG_A
Cuts at: 5343 5343
Size: 5680
BspHI T'CATG_A
Cuts at: 3569 5299 3569
Size: 1730 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2125 3035 844
Size: 1281 910 3489
Fragments arranged by size:
3489 1281 910
BsrBI CCG'CTC
Cuts at: 1394 1823 2260 2311 2790 2797 3567 1394
Size: 429 437 51 479 7 770 3507
Fragments arranged by size:
3507 770 479 437 429 51 7
BsrDI GCAATG_nn'
Cuts at: 65 1248 4175 4349 65
Size: 1183 2927 174 1396
Fragments arranged by size:
2927 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2493 2595 2693 4324 4511 1700
Size: 110 683 102 98 1631 187 2869
Fragments arranged by size:
2869 1631 683 187 110 102 98
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5680
BssHII G'CGCG_C
Cuts at: 1925 2416 1925
Size: 491 5189
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2042 2299 2662 794
Size: 1248 257 363 3812
Fragments arranged by size:
3812 1248 363 257
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 2206 2563 1184
Size: 1022 357 4301
Fragments arranged by size:
4301 1022 357
BstXI CCAn_nnnn'nTGG
Cuts at: 2197 2197
Size: 5680
BstYI r'GATC_y
Cuts at: 1972 2352 3010 3377 3762 3779 5231 5317 5329
Size: 380 658 367 385 17 1452 86 12
Cuts at: 5329 5675 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 658 385 380 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2169 2847 513
Size: 552 939 165 678 3346
Fragments arranged by size:
3346 939 678 552 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2092 2752 487
Size: 683 56 866 660 3415
Fragments arranged by size:
3415 866 683 660 56
BtsI GCAGTG_nn'
Cuts at: 3196 3990 4010 3196
Size: 794 20 4866
Fragments arranged by size:
4866 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5645
DraI TTT'AAA
Cuts at: 2984 2984
Size: 5680
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4687 809
Size: 507 3371 1802
Fragments arranged by size:
3371 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2268 2403 3127
Size: 54 1425 50 275 18 438 135 724
Cuts at: 3127 4016 5645 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 724 438 275 135 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2403 3127 1537
Size: 275 591 724 4090
Fragments arranged by size:
4090 724 591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2804 3610 1353
Size: 1451 806 3423
Fragments arranged by size:
3423 1451 806
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2680 4267 4641 4787 387
Size: 1064 138 1091 1587 374 146 1280
Fragments arranged by size:
1587 1280 1091 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2204 2306 2554 2663 2699 2785
Size: 393 485 87 102 248 109 36 86
Cuts at: 2785 3743 4340 5127 5395 1239
Size: 958 597 787 268 1524
Fragments arranged by size:
1524 958 787 597 485 393 268 248
109 102 87 86 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2645 2645
Size: 5680
EcoO109I rG'GnC_Cy
Cuts at: 2239 2725 2239
Size: 486 5194
EcoRI G'AATT_C
Cuts at: 2989 3367 2989
Size: 378 5302
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2932 2932
Size: 5680
FspI TGC'GCA
Cuts at: 2615 2666 2888 4186 2615
Size: 51 222 1298 4109
Fragments arranged by size:
4109 1298 222 51
HaeII r_GCGC'y
Cuts at: 1678 2278 2654 4827 1678
Size: 600 376 2173 2531
Fragments arranged by size:
2531 2173 600 376
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 4321 4353 4395
Size: 32 135 32 599 32 2118 32 42
Cuts at: 4395 4427 1373
Size: 32 2626
Fragments arranged by size:
2626 2118 599 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 3270 669
Size: 988 281 1086 246 3079
Fragments arranged by size:
3079 1086 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5680
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KpnI G_GTAC'C
Cuts at: 3005 3005
Size: 5680
MfeI C'AATT_G
Cuts at: 3279 3279
Size: 5680
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4794 4978 1305
Size: 3489 184 2007
Fragments arranged by size:
3489 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2270 10
Size: 54 1425 343 438 3420
Fragments arranged by size:
3420 1425 438 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3638 3997 4156 518
Size: 648 2472 359 159 2042
Fragments arranged by size:
2472 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2077 2266 2296 2317 2332 2892 2931 3096
Size: 471 189 30 21 15 560 39 165
Cuts at: 3096 3773 4921 5166 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 560 471 245 189 165
39 30 21 15
NaeI GCC'GGC
Cuts at: 2695 2695
Size: 5680
NcoI C'CATG_G
Cuts at: 513 1065 2004 2847 513
Size: 552 939 843 3346
Fragments arranged by size:
3346 939 843 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NgoMIV G'CCGG_C
Cuts at: 2693 2693
Size: 5680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 3038 4583 1132
Size: 97 1809 1545 2229
Fragments arranged by size:
2229 1809 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5680
PciI A'CATG_T
Cuts at: 4579 4579
Size: 5680
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2284 2931 1108
Size: 1176 647 3857
Fragments arranged by size:
3857 1176 647
PfoI T'CCnGG_A
Cuts at: 2676 2676
Size: 5680
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2725 2725
Size: 5680
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5680
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PstI C_TGCA'G
Cuts at: 830 2304 2658 3032 830
Size: 1474 354 374 3478
Fragments arranged by size:
3478 1474 374 354
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
PvuII CAG'CTG
Cuts at: 2266 2892 2266
Size: 626 5054
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SapI GCTCTTCn'nnn_
Cuts at: 2804 2804
Size: 5680
SbfI CC_TGCA'GG
Cuts at: 3032 3032
Size: 5680
ScaI AGT'ACT
Cuts at: 3928 5640 3928
Size: 1712 3968
SfcI C'TryA_G
Cuts at: 826 1045 2300 2654 3028 4163 4844 5035 826
Size: 219 1255 354 374 1135 681 191 1471
Fragments arranged by size:
1471 1255 1135 681 374 354 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2402 2402
Size: 5680
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 2595 2693 1700
Size: 895 98 4687
Fragments arranged by size:
4687 895 98
SmaI CCC'GGG
Cuts at: 1872 3007 1872
Size: 1135 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2057 2126 3784 4685 4947 820
Size: 197 926 114 69 1658 901 262 1553
Fragments arranged by size:
1658 1553 926 901 262 197 114 69
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 3038 1132
Size: 1906 3774
SspI AAT'ATT
Cuts at: 5 52 3604 5
Size: 47 3552 2081
Fragments arranged by size:
3552 2081 47
StuI AGG'CCT
Cuts at: 1517 2939 1517
Size: 1422 4258
StyI C'CwwG_G
Cuts at: 513 1065 2004 2847 3119 513
Size: 552 939 843 272 3074
Fragments arranged by size:
3074 939 843 552 272
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3707 3724 3877 4062 4507 325
Size: 1255 2127 17 153 185 445 1498
Fragments arranged by size:
2127 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2013 2967 3926
Size: 274 80 33 51 1443 36 954 959
Cuts at: 3926 5638 96
Size: 1712 138
Fragments arranged by size:
1712 1443 959 954 274 138 80 51
36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5640 5672 5640
Size: 32 5648
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5680
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5680
XmaI C'CCGG_G
Cuts at: 1870 3005 1870
Size: 1135 4545
XmnI GAAnn'nnTTC
Cuts at: 3809 3809
Size: 5680
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfoI
SrfI SwaI XcmI