Restriction map for
pFN21AB9826
(Circular) (Six-base) MAPSORT of: pFN21AB9826.seq Check: 1145 from: 1 to: 5668
pFN21AB9826 5668 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3478 278
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3011 1937
Size: 1074 4594
Acc65I G'GTAC_C
Cuts at: 2989 2989
Size: 5668
AclI AA'CG_TT
Cuts at: 1563 3795 4168 1563
Size: 2232 373 3063
Fragments arranged by size:
3063 2232 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2759 3731 5115 5383 1632
Size: 1127 972 1384 268 1917
Fragments arranged by size:
1917 1384 1127 972 268
AfeI AGC'GCT
Cuts at: 2514 2514
Size: 5668
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5471
AflIII A'CryG_T
Cuts at: 1203 4567 5544 1203
Size: 3364 977 1327
Fragments arranged by size:
3364 1327 977
AgeI A'CCGG_T
Cuts at: 4499 4499
Size: 5668
AhdI GACnn_n'nnGTC
Cuts at: 4397 4397
Size: 5668
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2238 2573 1184
Size: 1054 335 4279
Fragments arranged by size:
4279 1054 335
AlwNI CAG_nnn'CTG
Cuts at: 3411 4983 3411
Size: 1572 4096
ApaI G_GGCC'C
Cuts at: 2273 2273
Size: 5668
ApaLI G'TGCA_C
Cuts at: 2069 3725 4881 2069
Size: 1656 1156 2856
Fragments arranged by size:
2856 1656 1156
ApoI r'AATT_y
Cuts at: 1493 2977 3203 3355 5464 1493
Size: 1484 226 152 2109 1697
Fragments arranged by size:
2109 1697 1484 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4222 160
Size: 872 3190 1606
Fragments arranged by size:
3190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
AvaI C'yCGr_G
Cuts at: 1870 1943 2251 2448 2522 2796 2993 1870
Size: 73 308 197 74 274 197 4545
Fragments arranged by size:
4545 308 274 197 197 74 73
BamHI G'GATC_C
Cuts at: 2998 3365 2998
Size: 367 5301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2275 2615 2989 4444 618
Size: 325 217 627 488 340 374 1455 1842
Fragments arranged by size:
1842 1455 627 488 374 340 325 217
BanII G_rGCy'C
Cuts at: 1391 2135 2273 2319 2523 2795 2914 2987 1391
Size: 744 138 46 204 272 119 73 4072
Fragments arranged by size:
4072 744 272 204 138 119 73 46
BbeI G_GCGC'C
Cuts at: 2619 2619
Size: 5668
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4697
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2194 2228 3859 3893 1903
Size: 34 257 34 1631 34 3678
Fragments arranged by size:
3678 1631 257 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3560 4770 3560
Size: 1210 4458
BclI T'GATC_A
Cuts at: 1548 2355 1548
Size: 807 4861
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2246 4279 136
Size: 107 122 71 631 605 574 2033 1525
Fragments arranged by size:
2033 1525 631 605 574 122 107 71
BglII A'GATC_T
Cuts at: 5663 5663
Size: 5668
BlpI GC'TnA_GC
Cuts at: 2546 3085 2546
Size: 539 5129
Bme1580I G_kGCm'C
Cuts at: 1790 2073 2273 3729 4885 1790
Size: 283 200 1456 1156 2573
Fragments arranged by size:
2573 1456 1156 283 200
BmgBI CAC'GTC
Cuts at: 1318 2695 1318
Size: 1377 4291
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3399 4357 442
Size: 416 53 83 399 24 1982 958 1753
Fragments arranged by size:
1982 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5668
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2154 2154
Size: 5668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2537 2918 4328 1239
Size: 1298 381 1410 2579
Fragments arranged by size:
2579 1410 1298 381
Bpu10I CC'TnA_GC
Cuts at: 2588 2588
Size: 5668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3793 4658 4956 3793
Size: 865 298 4505
Fragments arranged by size:
4505 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4331 882
Size: 617 2832 2219
Fragments arranged by size:
2832 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2329 2602 2761 3459 493
Size: 711 1125 273 159 698 2702
Fragments arranged by size:
2702 1125 711 698 273 159
BsaBI GATnn'nnATC
Cuts at: 2357 2825 5662 2357
Size: 468 2837 2363
Fragments arranged by size:
2837 2363 468
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2616 3475 3857 4492
Size: 53 83 186 936 1083 859 382 635
Cuts at: 4492 275
Size: 1451
Fragments arranged by size:
1451 1083 936 859 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4101 4499 4773 4920 5331 4101
Size: 398 274 147 411 4438
Fragments arranged by size:
4438 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2140 2170 1178
Size: 30 165 30 737 30 4676
Fragments arranged by size:
4676 737 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2127 2243 2540 2729 2915 1186
Size: 941 116 297 189 186 3939
Fragments arranged by size:
3939 941 297 189 186 116
BseYI C'CCAG_C
Cuts at: 2262 4871 5367 2262
Size: 2609 496 2563
Fragments arranged by size:
2609 2563 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2321 2431 2447 2468 1163
Size: 573 585 110 16 21 4363
Fragments arranged by size:
4363 585 573 110 21 16
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2154 3118 3879 4907 1540
Size: 275 186 153 964 761 1028 2301
Fragments arranged by size:
2301 1028 964 761 275 186 153
BsiHKAI G_wGCw'C
Cuts at: 2073 2319 2914 3729 3814 4885 2073
Size: 246 595 815 85 1071 2856
Fragments arranged by size:
2856 1071 815 595 246 85
BsmI GAATG_Cn'
Cuts at: 3179 3272 3179
Size: 93 5575
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2135 2273 2319 2523 2795 2914
Size: 399 283 62 138 46 204 272 119
Cuts at: 2914 2987 3729 3814 4885 1391
Size: 73 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 283 272 204 138
119 85 73 62 46
BspEI T'CCGG_A
Cuts at: 5331 5331
Size: 5668
BspHI T'CATG_A
Cuts at: 2580 3557 5287 2580
Size: 977 1730 2961
Fragments arranged by size:
2961 1730 977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3023 844
Size: 2179 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2156 3555 1394
Size: 429 333 1399 3507
Fragments arranged by size:
3507 1399 429 333
BsrDI GCAATG_nn'
Cuts at: 65 1248 4163 4337 65
Size: 1183 2915 174 1396
Fragments arranged by size:
2915 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4312 4499 1700
Size: 110 2502 187 2869
Fragments arranged by size:
2869 2502 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5668
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5668
BssSI C'ACGA_G
Cuts at: 2312 3728 4740 2312
Size: 1416 1012 3240
Fragments arranged by size:
3240 1416 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2299 794
Size: 1505 4163
BstBI TT'CG_AA
Cuts at: 4472 4472
Size: 5668
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5668
BstYI r'GATC_y
Cuts at: 1972 2023 2998 3365 3750 3767 5219 5305 5317
Size: 51 975 367 385 17 1452 86 12
Cuts at: 5317 5663 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 975 385 367 346 86 51
17 12
Bsu36I CC'TnA_GG
Cuts at: 2468 2468
Size: 5668
BtgI C'CryG_G
Cuts at: 513 1065 2490 2611 513
Size: 552 1425 121 3570
Fragments arranged by size:
3570 1425 552 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4929
Fragments arranged by size:
4929 683 56
BtsI GCAGTG_nn'
Cuts at: 2931 2959 3184 3978 3998 2931
Size: 28 225 794 20 4601
Fragments arranged by size:
4601 794 225 28 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5633
DraI TTT'AAA
Cuts at: 2972 2972
Size: 5668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4675 809
Size: 507 3359 1802
Fragments arranged by size:
3359 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2151 2841 3115
Size: 54 1425 50 275 18 321 690 274
Cuts at: 3115 4004 5633 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 690 321 275 274 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2151 3115 1537
Size: 275 339 964 4090
Fragments arranged by size:
4090 964 339 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3598 1353
Size: 2245 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4255 4629 4775 387
Size: 1064 138 2666 374 146 1280
Fragments arranged by size:
2666 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2317 2912 2317
Size: 595 5073
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2537 2759 2918 3731 4328 5115 5383
Size: 393 905 222 159 813 597 787 268
Cuts at: 5383 1239
Size: 1524
Fragments arranged by size:
1524 905 813 787 597 393 268 222
159
EcoNI CCTnn'n_nnAGG
Cuts at: 2139 2216 2139
Size: 77 5591
EcoO109I rG'GnC_Cy
Cuts at: 2053 2182 2209 2248 2053
Size: 129 27 39 5473
Fragments arranged by size:
5473 129 39 27
EcoRI G'AATT_C
Cuts at: 2977 3355 2977
Size: 378 5290
FspI TGC'GCA
Cuts at: 2648 4174 2648
Size: 1526 4142
HaeII r_GCGC'y
Cuts at: 1678 2516 2546 2619 2631 4815 1678
Size: 838 30 73 12 2184 2531
Fragments arranged by size:
2531 2184 838 73 30 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2140 2172 4309 4341 4383
Size: 32 135 32 568 32 2137 32 42
Cuts at: 4383 4415 1373
Size: 32 2626
Fragments arranged by size:
2626 2137 568 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3012 3258 669
Size: 988 281 1074 246 3079
Fragments arranged by size:
3079 1074 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5668
HpaI GTT'AAC
Cuts at: 3258 3258
Size: 5668
KasI G'GCGC_C
Cuts at: 2615 2615
Size: 5668
KpnI G_GTAC'C
Cuts at: 2993 2993
Size: 5668
MfeI C'AATT_G
Cuts at: 3267 3267
Size: 5668
MluI A'CGCG_T
Cuts at: 5544 5544
Size: 5668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4782 4966 1305
Size: 3477 184 2007
Fragments arranged by size:
3477 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2843 10
Size: 54 1425 343 1011 2835
Fragments arranged by size:
2835 1425 1011 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2561 2600 2819 3626 3985 4144 518
Size: 648 1395 39 219 807 359 159 2042
Fragments arranged by size:
2042 1395 807 648 359 219 159 39
MspA1I CmG'CkG
Cuts at: 1606 2296 2613 3084 3761 4909 5154 1606
Size: 690 317 471 677 1148 245 2120
Fragments arranged by size:
2120 1148 690 677 471 317 245
NarI GG'CG_CC
Cuts at: 2616 2616
Size: 5668
NcoI C'CATG_G
Cuts at: 513 1065 2490 513
Size: 552 1425 3691
Fragments arranged by size:
3691 1425 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5668
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5668
NotI GC'GGCC_GC
Cuts at: 3115 3115
Size: 5668
NspI r_CATG'y
Cuts at: 1132 1229 2560 3026 4571 1132
Size: 97 1331 466 1545 2229
Fragments arranged by size:
2229 1545 1331 466 97
PasI CC'CwG_GG
Cuts at: 1348 2081 1348
Size: 733 4935
PciI A'CATG_T
Cuts at: 4567 4567
Size: 5668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5668
PmeI GTTT'AAAC
Cuts at: 2972 2972
Size: 5668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 5250 5282 3737
Size: 32 1481 32 4123
Fragments arranged by size:
4123 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5668
PsiI TTA'TAA
Cuts at: 3238 3238
Size: 5668
PspOMI G'GGCC_C
Cuts at: 2269 2269
Size: 5668
PstI C_TGCA'G
Cuts at: 830 2217 3020 830
Size: 1387 803 3478
Fragments arranged by size:
3478 1387 803
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5668
PvuII CAG'CTG
Cuts at: 2296 2296
Size: 5668
SacI G_AGCT'C
Cuts at: 2319 2914 2319
Size: 595 5073
SacII CC_GC'GG
Cuts at: 2614 2614
Size: 5668
SalI G'TCGA_C
Cuts at: 1936 3010 1936
Size: 1074 4594
SbfI CC_TGCA'GG
Cuts at: 3020 3020
Size: 5668
ScaI AGT'ACT
Cuts at: 3916 5628 3916
Size: 1712 3956
SfcI C'TryA_G
Cuts at: 826 1045 2213 3016 4151 4832 5023 826
Size: 219 1168 803 1135 681 191 1471
Fragments arranged by size:
1471 1168 1135 803 681 219 191
SfoI GGC'GCC
Cuts at: 2617 2617
Size: 5668
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5668
SmaI CCC'GGG
Cuts at: 1872 2450 2798 2995 1872
Size: 578 348 197 4545
Fragments arranged by size:
4545 578 348 197
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3772 4673 4935 820
Size: 197 926 1829 901 262 1553
Fragments arranged by size:
1829 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3459 493
Size: 2966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5668
SphI G_CATG'C
Cuts at: 1132 2560 3026 1132
Size: 1428 466 3774
Fragments arranged by size:
3774 1428 466
SspI AAT'ATT
Cuts at: 5 52 3592 5
Size: 47 3540 2081
Fragments arranged by size:
3540 2081 47
StuI AGG'CCT
Cuts at: 1517 2571 2870 1517
Size: 1054 299 4315
Fragments arranged by size:
4315 1054 299
StyI C'CwwG_G
Cuts at: 513 1065 2490 3107 513
Size: 552 1425 617 3074
Fragments arranged by size:
3074 1425 617 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3695 3712 3865 4050 4495 325
Size: 1255 2115 17 153 185 445 1498
Fragments arranged by size:
2115 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3914 5626 96
Size: 274 80 33 51 1443 1937 1712 138
Fragments arranged by size:
1937 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5628 5660 5628
Size: 32 5636
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5668
XbaI T'CTAG_A
Cuts at: 3004 3004
Size: 5668
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5668
XmaI C'CCGG_G
Cuts at: 1870 2448 2796 2993 1870
Size: 578 348 197 4545
Fragments arranged by size:
4545 578 348 197
XmnI GAAnn'nnTTC
Cuts at: 3797 3797
Size: 5668
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3476 276
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI
SwaI XcmI