Restriction map for
pFN21AB9825
(Circular) (Six-base) MAPSORT of: pFN21AB9825.seq Check: 6815 from: 1 to: 5656
pFN21AB9825 5656 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2555 2555
Size: 5656
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2999 1937
Size: 1062 4594
Acc65I G'GTAC_C
Cuts at: 2788 2977 2788
Size: 189 5467
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2501 3719 5103 5371 1632
Size: 869 1218 1384 268 1917
Fragments arranged by size:
1917 1384 1218 869 268
AfeI AGC'GCT
Cuts at: 2441 2441
Size: 5656
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 4555 5532 1203
Size: 3352 977 1327
Fragments arranged by size:
3352 1327 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 2311 4385 2311
Size: 2074 3582
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2133 1184
Size: 949 4707
AlwNI CAG_nnn'CTG
Cuts at: 3399 4971 3399
Size: 1572 4084
ApaLI G'TGCA_C
Cuts at: 3713 4869 3713
Size: 1156 4500
ApoI r'AATT_y
Cuts at: 1493 2965 3191 3343 5452 1493
Size: 1472 226 152 2109 1697
Fragments arranged by size:
2109 1697 1472 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2981 1870
Size: 73 1038 4545
Fragments arranged by size:
4545 1038 73
BamHI G'GATC_C
Cuts at: 2986 3353 2986
Size: 367 5289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 2788 2977 4432 618
Size: 325 217 627 363 638 189 1455 1842
Fragments arranged by size:
1842 1455 638 627 363 325 217 189
BanII G_rGCy'C
Cuts at: 1391 2733 2839 2975 1391
Size: 1342 106 136 4072
Fragments arranged by size:
4072 1342 136 106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2001 928
Size: 971 102 4583
Fragments arranged by size:
4583 971 102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3847 3881 1903
Size: 34 1910 34 3678
Fragments arranged by size:
3678 1910 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5656
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4267 136
Size: 107 122 71 631 605 2595 1525
Fragments arranged by size:
2595 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2589 5651 2589
Size: 3062 2594
BlpI GC'TnA_GC
Cuts at: 3073 3073
Size: 5656
Bme1580I G_kGCm'C
Cuts at: 1790 2630 3717 4873 1790
Size: 840 1087 1156 2573
Fragments arranged by size:
2573 1156 1087 840
BmgBI CAC'GTC
Cuts at: 1318 2503 1318
Size: 1185 4471
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3387 4345 442
Size: 416 53 83 399 24 1970 958 1753
Fragments arranged by size:
1970 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2348 2348
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2177 2266 2435 2795 2888 4316 1239
Size: 938 89 169 360 93 1428 2579
Fragments arranged by size:
2579 1428 938 360 169 93 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3781 4646 4944 3781
Size: 865 298 4493
Fragments arranged by size:
4493 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4319 882
Size: 617 2820 2219
Fragments arranged by size:
2820 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3447 493
Size: 711 2243 2702
Fragments arranged by size:
2702 2243 711
BsaBI GATnn'nnATC
Cuts at: 5650 5650
Size: 5656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3463 3845 4480 275
Size: 53 83 186 936 1930 382 635 1451
Fragments arranged by size:
1930 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4089 4487 4761 4908 5319 4089
Size: 398 274 147 411 4426
Fragments arranged by size:
4426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5431
Fragments arranged by size:
5431 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2116 2119 2120 2122 2127 2297 2675 2690
Size: 930 3 1 2 5 170 378 15
Cuts at: 2690 2954 1186
Size: 264 3888
Fragments arranged by size:
3888 930 378 264 170 15 5 3
2 1
BseYI C'CCAG_C
Cuts at: 2575 2680 4859 5355 2575
Size: 105 2179 496 2876
Fragments arranged by size:
2876 2179 496 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2780 1163
Size: 573 1044 4039
Fragments arranged by size:
4039 1044 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3106 3867 4895 1540
Size: 275 186 1105 761 1028 2301
Fragments arranged by size:
2301 1105 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2715 3717 3802 4873 2715
Size: 1002 85 1071 3498
Fragments arranged by size:
3498 1071 1002 85
BsmI GAATG_Cn'
Cuts at: 3167 3260 3167
Size: 93 5563
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2630 2715 2733 2839 2975 3717 3802
Size: 399 840 85 18 106 136 742 85
Cuts at: 3802 4873 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 840 742 399 136 106 85
85 18
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 3545 5275 3545
Size: 1730 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2174 2555 2776 3011 844
Size: 1330 381 221 235 3489
Fragments arranged by size:
3489 1330 381 235 221
BsrBI CCG'CTC
Cuts at: 1394 1823 3543 1394
Size: 429 1720 3507
Fragments arranged by size:
3507 1720 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2643 2901 4151 4325 65
Size: 1183 1395 258 1250 174 1396
Fragments arranged by size:
1396 1395 1250 1183 258 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2781 4300 4487 1700
Size: 110 971 1519 187 2869
Fragments arranged by size:
2869 1519 971 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5656
BssHII G'CGCG_C
Cuts at: 1925 2807 1925
Size: 882 4774
BssSI C'ACGA_G
Cuts at: 3716 4728 3716
Size: 1012 4644
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5656
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstXI CCAn_nnnn'nTGG
Cuts at: 2392 2392
Size: 5656
BstYI r'GATC_y
Cuts at: 1972 2589 2986 3353 3738 3755 5207 5293 5305
Size: 617 397 367 385 17 1452 86 12
Cuts at: 5305 5651 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 617 397 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2040 2202 2040
Size: 162 5494
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4917
Fragments arranged by size:
4917 683 56
BtsI GCAGTG_nn'
Cuts at: 2029 3172 3966 3986 2029
Size: 1143 794 20 3699
Fragments arranged by size:
3699 1143 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5621
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4663 809
Size: 507 3347 1802
Fragments arranged by size:
3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3103 3992 5621
Size: 54 1425 50 275 18 1273 889 1629
Cuts at: 5621 8
Size: 43
Fragments arranged by size:
1629 1425 1273 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3103 1537
Size: 275 1291 4090
Fragments arranged by size:
4090 1291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2015 2058 2274 2940 3586 1353
Size: 662 43 216 666 646 3423
Fragments arranged by size:
3423 666 662 646 216 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4243 4617 4763 387
Size: 1064 138 2654 374 146 1280
Fragments arranged by size:
2654 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2177 2266 2435 2501 2795 2888 3719
Size: 393 545 89 169 66 294 93 831
Cuts at: 3719 4316 5103 5371 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 831 787 597 545 393 294 268
169 93 89 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2043 2043
Size: 5656
EcoO109I rG'GnC_Cy
Cuts at: 2068 2186 2068
Size: 118 5538
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2461 2506 2461
Size: 45 5611
FspI TGC'GCA
Cuts at: 2088 4162 2088
Size: 2074 3582
HaeII r_GCGC'y
Cuts at: 1678 2443 2655 4803 1678
Size: 765 212 2148 2531
Fragments arranged by size:
2531 2148 765 212
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2297 2329 2639 2671 4297
Size: 32 135 32 725 32 310 32 1626
Cuts at: 4297 4329 4371 4403 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1626 725 310 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3000 3246 669
Size: 988 281 1062 246 3079
Fragments arranged by size:
3079 1062 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5656
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KpnI G_GTAC'C
Cuts at: 2792 2981 2792
Size: 189 5467
MfeI C'AATT_G
Cuts at: 2631 3255 2631
Size: 624 5032
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4770 4954 1305
Size: 3465 184 2007
Fragments arranged by size:
3465 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3834
Fragments arranged by size:
3834 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2501 3614 3973 4132 518
Size: 648 1335 1113 359 159 2042
Fragments arranged by size:
2042 1335 1113 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3072 3749 4897 5142 1606
Size: 1466 677 1148 245 2120
Fragments arranged by size:
2120 1466 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5104
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 2876 3014 4559 1132
Size: 97 1647 138 1545 2229
Fragments arranged by size:
2229 1647 1545 138 97
PasI CC'CwG_GG
Cuts at: 1348 2071 1348
Size: 723 4933
PciI A'CATG_T
Cuts at: 4555 4555
Size: 5656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5656
PfoI T'CCnGG_A
Cuts at: 2865 2865
Size: 5656
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2303 2335 3725 3757 5238 5270 2303
Size: 32 1390 32 1481 32 2689
Fragments arranged by size:
2689 1481 1390 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2186 2186
Size: 5656
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5656
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PstI C_TGCA'G
Cuts at: 830 3008 830
Size: 2178 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SfcI C'TryA_G
Cuts at: 826 1045 3004 4139 4820 5011 826
Size: 219 1959 1135 681 191 1471
Fragments arranged by size:
1959 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2983 1872
Size: 1111 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3760 4661 4923 820
Size: 197 926 1817 901 262 1553
Fragments arranged by size:
1817 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 2607 1517
Size: 1090 4566
StyI C'CwwG_G
Cuts at: 513 1065 2385 2831 3095 513
Size: 552 1320 446 264 3074
Fragments arranged by size:
3074 1320 552 446 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2072 2672 2746 2762 3683 3700 3853
Size: 1255 492 600 74 16 921 17 153
Cuts at: 3853 4038 4483 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 921 600 492 445 185 153
74 17 16
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3902 5614 96
Size: 274 80 33 51 1443 1925 1712 138
Fragments arranged by size:
1925 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5616 5648 5616
Size: 32 5624
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5656
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5656
XmaI C'CCGG_G
Cuts at: 1870 2981 1870
Size: 1111 4545
XmnI GAAnn'nnTTC
Cuts at: 3785 3785
Size: 5656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI