Restriction map for
pFN21AB9822
(Circular) (Six-base) MAPSORT of: pFN21AB9822.seq Check: 5064 from: 1 to: 5641
pFN21AB9822 5641 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2984 1937
Size: 1047 4594
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3704 5088 5356 1632
Size: 2072 1384 268 1917
Fragments arranged by size:
2072 1917 1384 268
AfeI AGC'GCT
Cuts at: 2138 2696 2138
Size: 558 5083
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 4540 5517 1203
Size: 3337 977 1327
Fragments arranged by size:
3337 1327 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 2104 3384 4956 2104
Size: 1280 1572 2789
Fragments arranged by size:
2789 1572 1280
ApaI G_GGCC'C
Cuts at: 2300 2486 2636 2300
Size: 186 150 5305
Fragments arranged by size:
5305 186 150
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2369 2950 3176 3328 5437 1493
Size: 876 581 226 152 2109 1697
Fragments arranged by size:
2109 1697 876 581 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2309 2486 2614 2966 1870
Size: 73 366 177 128 352 4545
Fragments arranged by size:
4545 366 352 177 128 73
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2327 2624 2669 2813 2962
Size: 325 217 627 540 297 45 144 149
Cuts at: 2962 4417 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 540 325 297 217 149
144 45
BanII G_rGCy'C
Cuts at: 1391 2300 2486 2636 2661 2960 1391
Size: 909 186 150 25 299 4072
Fragments arranged by size:
4072 909 299 186 150 25
BbeI G_GCGC'C
Cuts at: 2331 2628 2817 2331
Size: 297 189 5155
Fragments arranged by size:
5155 297 189
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4670
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2619 2640 4252
Size: 107 122 71 631 605 947 21 1612
Cuts at: 4252 136
Size: 1525
Fragments arranged by size:
1612 1525 947 631 605 122 107 71
21
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 3058 3058
Size: 5641
Bme1580I G_kGCm'C
Cuts at: 1790 2300 2486 2636 2672 3702 4858 1790
Size: 510 186 150 36 1030 1156 2573
Fragments arranged by size:
2573 1156 1030 510 186 150 36
BmgBI CAC'GTC
Cuts at: 1318 2885 1318
Size: 1567 4074
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3372 4330 442
Size: 416 53 83 399 24 1955 958 1753
Fragments arranged by size:
1955 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2125 2689 2125
Size: 564 5077
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4301 1239
Size: 3062 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2165 2345 2393 3766 4631 4929 2165
Size: 180 48 1373 865 298 2877
Fragments arranged by size:
2877 1373 865 298 180 48
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4304 882
Size: 617 2805 2219
Fragments arranged by size:
2805 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3432 493
Size: 711 2228 2702
Fragments arranged by size:
2702 2228 711
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2328 2625 2814 3448
Size: 53 83 186 936 795 297 189 634
Cuts at: 3448 3830 4465 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 795 635 634 382 297 189
186 83 53
BsaWI w'CCGG_w
Cuts at: 4074 4472 4746 4893 5304 4074
Size: 398 274 147 411 4411
Fragments arranged by size:
4411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5416
Fragments arranged by size:
5416 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 2939 1186
Size: 1162 591 3888
Fragments arranged by size:
3888 1162 591
BseYI C'CCAG_C
Cuts at: 2263 2293 4844 5340 2263
Size: 30 2551 496 2564
Fragments arranged by size:
2564 2551 496 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5068
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2428 3091 3852 4880 1540
Size: 275 186 427 663 761 1028 2301
Fragments arranged by size:
2301 1028 761 663 427 275 186
BsiHKAI G_wGCw'C
Cuts at: 2661 3702 3787 4858 2661
Size: 1041 85 1071 3444
Fragments arranged by size:
3444 1071 1041 85
BsmI GAATG_Cn'
Cuts at: 3152 3245 3152
Size: 93 5548
BsmBI CGTCTCn'nnnn_
Cuts at: 2087 2087
Size: 5641
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2300 2486 2636 2661 2672 2960 3702
Size: 399 510 186 150 25 11 288 742
Cuts at: 3702 3787 4858 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 510 399 288 186 150
85 25 11
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 3530 5260 3530
Size: 1730 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2996 844
Size: 2152 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2508 3528 1394
Size: 429 685 1020 3507
Fragments arranged by size:
3507 1020 685 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2776 4136 4310 65
Size: 1183 1528 1360 174 1396
Fragments arranged by size:
1528 1396 1360 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2823 4285 4472 1700
Size: 110 1013 1462 187 2869
Fragments arranged by size:
2869 1462 1013 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 2353 2355 1925
Size: 428 2 5211
Fragments arranged by size:
5211 428 2
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5641
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5641
BstXI CCAn_nnnn'nTGG
Cuts at: 2239 2683 2239
Size: 444 5197
BstYI r'GATC_y
Cuts at: 1972 2971 3338 3723 3740 5192 5278 5290 5636
Size: 999 367 385 17 1452 86 12 346
Cuts at: 5636 1972
Size: 1977
Fragments arranged by size:
1977 1452 999 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2074 2232 2348 2458 2682 513
Size: 552 939 70 158 116 110 224 3472
Fragments arranged by size:
3472 939 552 224 158 116 110 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2503 487
Size: 683 56 1277 3625
Fragments arranged by size:
3625 1277 683 56
BtsI GCAGTG_nn'
Cuts at: 3157 3951 3971 3157
Size: 794 20 4827
Fragments arranged by size:
4827 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2241 2425 2509
Size: 54 1425 50 275 18 411 184 84
Cuts at: 2509 3088 3977 5606 8
Size: 579 889 1629 43
Fragments arranged by size:
1629 1425 889 579 411 275 184 84
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2425 3088 1537
Size: 275 613 663 4090
Fragments arranged by size:
4090 663 613 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2904 3571 1353
Size: 1551 667 3423
Fragments arranged by size:
3423 1551 667
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2432 4228 4602 4748 387
Size: 1064 138 435 408 1796 374 146 1280
Fragments arranged by size:
1796 1280 1064 435 408 374 146 138
EcoICRI GAG'CTC
Cuts at: 2659 2659
Size: 5641
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3704 4301 5088 5356 1239
Size: 393 2072 597 787 268 1524
Fragments arranged by size:
2072 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2297 2483 2536 2632 2297
Size: 186 53 96 5306
Fragments arranged by size:
5306 186 96 53
EcoRI G'AATT_C
Cuts at: 2369 2950 3328 2369
Size: 581 378 4682
Fragments arranged by size:
4682 581 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2191 2716 2191
Size: 525 5116
FspI TGC'GCA
Cuts at: 2438 2532 4147 2438
Size: 94 1615 3932
Fragments arranged by size:
3932 1615 94
HaeII r_GCGC'y
Cuts at: 1678 2140 2331 2628 2698 2817 4788 1678
Size: 462 191 297 70 119 1971 2531
Fragments arranged by size:
2531 1971 462 297 191 119 70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373
Size: 32 135 32 2710 32 42 32 2626
Fragments arranged by size:
2710 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2455 2985 3231 669
Size: 988 281 517 530 246 3079
Fragments arranged by size:
3079 988 530 517 281 246
HindIII A'AGCT_T
Cuts at: 748 2870 748
Size: 2122 3519
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KasI G'GCGC_C
Cuts at: 2327 2624 2813 2327
Size: 297 189 5155
Fragments arranged by size:
5155 297 189
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4755 4939 1305
Size: 3450 184 2007
Fragments arranged by size:
3450 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3819
Fragments arranged by size:
3819 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 2681 3599 3958 4117 518
Size: 648 1071 444 918 359 159 2042
Fragments arranged by size:
2042 1071 918 648 444 359 159
MspA1I CmG'CkG
Cuts at: 1606 2022 2076 2293 2317 2350 2460 2898 3057
Size: 416 54 217 24 33 110 438 159
Cuts at: 3057 3734 4882 5127 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 438 416 245 217 159
110 54 33 24
NarI GG'CG_CC
Cuts at: 2328 2625 2814 2328
Size: 297 189 5155
Fragments arranged by size:
5155 297 189
NcoI C'CATG_G
Cuts at: 513 1065 2004 2232 513
Size: 552 939 228 3922
Fragments arranged by size:
3922 939 552 228
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 2425 3088 2425
Size: 663 4978
NspI r_CATG'y
Cuts at: 1132 1229 2999 4544 1132
Size: 97 1770 1545 2229
Fragments arranged by size:
2229 1770 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2636 1348
Size: 1288 4353
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5641
PfoI T'CCnGG_A
Cuts at: 2661 2850 2661
Size: 189 5452
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PspOMI G'GGCC_C
Cuts at: 2296 2482 2632 2296
Size: 186 150 5305
Fragments arranged by size:
5305 186 150
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2378 2410 2378
Size: 32 5609
PstI C_TGCA'G
Cuts at: 830 2993 830
Size: 2163 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
PvuII CAG'CTG
Cuts at: 2293 2317 2293
Size: 24 5617
SacI G_AGCT'C
Cuts at: 2661 2661
Size: 5641
SacII CC_GC'GG
Cuts at: 2077 2351 2461 2077
Size: 274 110 5257
Fragments arranged by size:
5257 274 110
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 2396 3889 5601 2396
Size: 1493 1712 2436
Fragments arranged by size:
2436 1712 1493
SfcI C'TryA_G
Cuts at: 826 1045 2989 4124 4805 4996 826
Size: 219 1944 1135 681 191 1471
Fragments arranged by size:
1944 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2329 2626 2815 2329
Size: 297 189 5155
Fragments arranged by size:
5155 297 189
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2488 2616 2968 1872
Size: 616 128 352 4545
Fragments arranged by size:
4545 616 352 128
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2180 2360 2408 3745 4646 4908
Size: 197 926 237 180 48 1337 901 262
Cuts at: 4908 820
Size: 1553
Fragments arranged by size:
1553 1337 926 901 262 237 197 180
48
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5641
StyI C'CwwG_G
Cuts at: 513 1065 2004 2166 2232 3080 513
Size: 552 939 162 66 848 3074
Fragments arranged by size:
3074 939 848 552 162 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3668 3685 3838 4023 4468 325
Size: 1255 2088 17 153 185 445 1498
Fragments arranged by size:
2088 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2394 3887 5599
Size: 274 80 33 51 1443 417 1493 1712
Cuts at: 5599 96
Size: 138
Fragments arranged by size:
1712 1493 1443 417 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5601 5633 5601
Size: 32 5609
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2486 2614 2966 1870
Size: 616 128 352 4545
Fragments arranged by size:
4545 616 352 128
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI