Restriction map for
pFN21AB9821
(Circular) (Six-base) MAPSORT of: pFN21AB9821.seq Check: 6474 from: 1 to: 5641
pFN21AB9821 5641 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2984 1937
Size: 1047 4594
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3704 5088 5356 1632
Size: 2072 1384 268 1917
Fragments arranged by size:
2072 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 4540 5517 1203
Size: 3337 977 1327
Fragments arranged by size:
3337 1327 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 2790 3384 4956 2790
Size: 594 1572 3475
Fragments arranged by size:
3475 1572 594
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2950 3176 3328 5437 1493
Size: 1457 226 152 2109 1697
Fragments arranged by size:
2109 1697 1457 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2458 2966 1870
Size: 73 515 508 4545
Fragments arranged by size:
4545 515 508 73
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2920 2962 4417 618
Size: 325 217 627 1133 42 1455 1842
Fragments arranged by size:
1842 1455 1133 627 325 217 42
BanII G_rGCy'C
Cuts at: 1391 2207 2255 2306 2753 2813 2960 1391
Size: 816 48 51 447 60 147 4072
Fragments arranged by size:
4072 816 447 147 60 51 48
BbeI G_GCGC'C
Cuts at: 2924 2924
Size: 5641
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2063 928
Size: 971 164 4506
Fragments arranged by size:
4506 971 164
BbvCI CC'TCA_GC
Cuts at: 2034 2034
Size: 5641
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2929 4252 136
Size: 107 122 71 631 605 1257 1323 1525
Fragments arranged by size:
1525 1323 1257 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 3058 3058
Size: 5641
Bme1580I G_kGCm'C
Cuts at: 1790 3702 4858 1790
Size: 1912 1156 2573
Fragments arranged by size:
2573 1912 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2036 3372 4330
Size: 416 53 83 399 24 619 1336 958
Cuts at: 4330 442
Size: 1753
Fragments arranged by size:
1753 1336 958 619 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2302 2377 2521 2713 2856 4301 1239
Size: 1063 75 144 192 143 1445 2579
Fragments arranged by size:
2579 1445 1063 192 144 143 75
Bpu10I CC'TnA_GC
Cuts at: 2034 2128 2034
Size: 94 5547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4631 4929 3766
Size: 865 298 4478
Fragments arranged by size:
4478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4304 882
Size: 617 2805 2219
Fragments arranged by size:
2805 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3432 493
Size: 711 2228 2702
Fragments arranged by size:
2702 2228 711
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2493 2886 2921 3448
Size: 53 83 186 936 960 393 35 527
Cuts at: 3448 3830 4465 275
Size: 382 635 1451
Fragments arranged by size:
1451 960 936 635 527 393 382 186
83 53 35
BsaWI w'CCGG_w
Cuts at: 2255 2276 2351 4074 4472 4746 4893 5304 2255
Size: 21 75 1723 398 274 147 411 2592
Fragments arranged by size:
2592 1723 411 398 274 147 75 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5416
Fragments arranged by size:
5416 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2197 2281 2338 2356 2401 2428 2500 2593
Size: 1011 84 57 18 45 27 72 93
Cuts at: 2593 2623 2761 2845 1186
Size: 30 138 84 3982
Fragments arranged by size:
3982 1011 138 93 84 84 72 57
45 30 27 18
BseYI C'CCAG_C
Cuts at: 2667 2907 4844 5340 2667
Size: 240 1937 496 2968
Fragments arranged by size:
2968 1937 496 240
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5068
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3852 4880 1540
Size: 275 186 1090 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2753 3702 3787 4858 2753
Size: 949 85 1071 3536
Fragments arranged by size:
3536 1071 949 85
BsmI GAATG_Cn'
Cuts at: 3152 3245 3152
Size: 93 5548
BsmBI CGTCTCn'nnnn_
Cuts at: 2500 2635 2500
Size: 135 5506
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2207 2255 2306 2753 2813 2960 3702
Size: 399 417 48 51 447 60 147 742
Cuts at: 3702 3787 4858 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 447 417 399 147 85
60 51 48
BspEI T'CCGG_A
Cuts at: 2276 5304 2276
Size: 3028 2613
BspHI T'CATG_A
Cuts at: 3530 5260 3530
Size: 1730 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2632 2996 844
Size: 1788 364 3489
Fragments arranged by size:
3489 1788 364
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4136 4310 65
Size: 1183 2888 174 1396
Fragments arranged by size:
2888 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4285 4472 1700
Size: 110 2475 187 2869
Fragments arranged by size:
2869 2475 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 2661 1925
Size: 736 4905
BssSI C'ACGA_G
Cuts at: 2656 3701 4713 2656
Size: 1045 1012 3584
Fragments arranged by size:
3584 1045 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2790 794
Size: 1996 3645
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5641
BstYI r'GATC_y
Cuts at: 1972 2971 3338 3723 3740 5192 5278 5290 5636
Size: 999 367 385 17 1452 86 12 346
Cuts at: 5636 1972
Size: 1977
Fragments arranged by size:
1977 1452 999 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2859 513
Size: 552 1794 3295
Fragments arranged by size:
3295 1794 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 3157 3951 3971 3157
Size: 794 20 4827
Fragments arranged by size:
4827 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2930 3088 3977
Size: 54 1425 50 275 18 1100 158 889
Cuts at: 3977 5606 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1100 889 275 158 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3571 1353
Size: 2218 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2610 2727 4228 4602 4748 387
Size: 1064 138 1021 117 1501 374 146 1280
Fragments arranged by size:
1501 1280 1064 1021 374 146 138 117
EcoICRI GAG'CTC
Cuts at: 2751 2751
Size: 5641
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2302 2377 2521 2713 2856 3704 4301
Size: 393 670 75 144 192 143 848 597
Cuts at: 4301 5088 5356 1239
Size: 787 268 1524
Fragments arranged by size:
1524 848 787 670 597 393 268 192
144 143 75
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 2924 4788 1678
Size: 1246 1864 2531
Fragments arranged by size:
2531 1864 1246
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373
Size: 32 135 32 2710 32 42 32 2626
Fragments arranged by size:
2710 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2985 3231 669
Size: 988 281 1047 246 3079
Fragments arranged by size:
3079 1047 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KasI G'GCGC_C
Cuts at: 2920 2920
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4755 4939 1305
Size: 3450 184 2007
Fragments arranged by size:
3450 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3819
Fragments arranged by size:
3819 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3599 3958 4117 518
Size: 648 2433 359 159 2042
Fragments arranged by size:
2433 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2091 2145 2322 2397 2541 2616 2709 2733
Size: 485 54 177 75 144 75 93 24
Cuts at: 2733 2787 3057 3734 4882 5127 1606
Size: 54 270 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 485 270 245 177 144
93 75 75 54 54 24
NarI GG'CG_CC
Cuts at: 2921 2921
Size: 5641
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5089
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2999 4544 1132
Size: 97 1770 1545 2229
Fragments arranged by size:
2229 1770 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5641
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5641
PfoI T'CCnGG_A
Cuts at: 2774 2870 2774
Size: 96 5545
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2131 2163 2131
Size: 32 5609
PstI C_TGCA'G
Cuts at: 830 2993 830
Size: 2163 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
SacI G_AGCT'C
Cuts at: 2753 2753
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 2273 3889 5601 2273
Size: 1616 1712 2313
Fragments arranged by size:
2313 1712 1616
SfcI C'TryA_G
Cuts at: 826 1045 2989 4124 4805 4996 826
Size: 219 1944 1135 681 191 1471
Fragments arranged by size:
1944 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2922 2922
Size: 5641
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2968 1872
Size: 1096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3745 4646 4908 820
Size: 197 926 1802 901 262 1553
Fragments arranged by size:
1802 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5641
StyI C'CwwG_G
Cuts at: 513 1065 3080 513
Size: 552 2015 3074
Fragments arranged by size:
3074 2015 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2852 3668 3685 3838 4023 4468 325
Size: 1255 1272 816 17 153 185 445 1498
Fragments arranged by size:
1498 1272 1255 816 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2271 3887 5599
Size: 274 80 33 51 1443 294 1616 1712
Cuts at: 5599 96
Size: 138
Fragments arranged by size:
1712 1616 1443 294 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5601 5633 5601
Size: 32 5609
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2966 1870
Size: 1096 4545
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PsrI PstI PvuI SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI