Restriction map for
pFN21AB9813
(Circular) (Six-base) MAPSORT of: pFN21AB9813.seq Check: 5228 from: 1 to: 5521
pFN21AB9813 5521 bp
With 182 enzymes: SgfI *
December 8, 2014 13:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3331 278
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2864 1937
Size: 927 4594
Acc65I G'GTAC_C
Cuts at: 2842 2842
Size: 5521
AclI AA'CG_TT
Cuts at: 1563 3648 4021 1563
Size: 2085 373 3063
Fragments arranged by size:
3063 2085 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2058 3584 4968 5236 1632
Size: 426 1526 1384 268 1917
Fragments arranged by size:
1917 1526 1384 426 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5324
AflIII A'CryG_T
Cuts at: 1203 4420 5397 1203
Size: 3217 977 1327
Fragments arranged by size:
3217 1327 977
AgeI A'CCGG_T
Cuts at: 4352 4352
Size: 5521
AhdI GACnn_n'nnGTC
Cuts at: 4250 4250
Size: 5521
AleI CACnn'nnGTG
Cuts at: 1166 2186 1166
Size: 1020 4501
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2204 1184
Size: 1020 4501
AlwNI CAG_nnn'CTG
Cuts at: 2779 3264 4836 2779
Size: 485 1572 3464
Fragments arranged by size:
3464 1572 485
ApaI G_GGCC'C
Cuts at: 2210 2577 2210
Size: 367 5154
ApaLI G'TGCA_C
Cuts at: 3578 4734 3578
Size: 1156 4365
ApoI r'AATT_y
Cuts at: 1493 2830 3056 3208 5317 1493
Size: 1337 226 152 2109 1697
Fragments arranged by size:
2109 1697 1337 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4075 160
Size: 872 3043 1606
Fragments arranged by size:
3043 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
AvaI C'yCGr_G
Cuts at: 1870 1943 2344 2435 2609 2738 2846 1870
Size: 73 401 91 174 129 108 4545
Fragments arranged by size:
4545 401 174 129 108 91 73
BamHI G'GATC_C
Cuts at: 2851 3218 2851
Size: 367 5154
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2083 2090 2180 2331 2804
Size: 325 217 627 296 7 90 151 473
Cuts at: 2804 2842 4297 618
Size: 38 1455 1842
Fragments arranged by size:
1842 1455 627 473 325 296 217 151
90 38 7
BanII G_rGCy'C
Cuts at: 1391 2159 2210 2577 2840 1391
Size: 768 51 367 263 4072
Fragments arranged by size:
4072 768 367 263 51
BbeI G_GCGC'C
Cuts at: 2087 2335 2808 2087
Size: 248 473 4800
Fragments arranged by size:
4800 473 248
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4550
BbvCI CC'TCA_GC
Cuts at: 2558 2558
Size: 5521
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3712 3746 1903
Size: 34 50 34 1691 34 3678
Fragments arranged by size:
3678 1691 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3413 4623 3413
Size: 1210 4311
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5521
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2587 2742 2802
Size: 107 122 71 631 605 915 155 60
Cuts at: 2802 4132 136
Size: 1330 1525
Fragments arranged by size:
1525 1330 915 631 605 155 122 107
71 60
BglII A'GATC_T
Cuts at: 5516 5516
Size: 5521
BlpI GC'TnA_GC
Cuts at: 2141 2938 2141
Size: 797 4724
Bme1580I G_kGCm'C
Cuts at: 1790 2210 2577 3582 4738 1790
Size: 420 367 1005 1156 2573
Fragments arranged by size:
2573 1156 1005 420 367
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5521
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3252 4210 442
Size: 416 53 83 399 24 1835 958 1753
Fragments arranged by size:
1835 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2414 2477 4181 1239
Size: 950 225 63 1704 2579
Fragments arranged by size:
2579 1704 950 225 63
Bpu10I CC'TnA_GC
Cuts at: 2558 2558
Size: 5521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3646 4511 4809 3646
Size: 865 298 4358
Fragments arranged by size:
4358 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4184 882
Size: 617 2685 2219
Fragments arranged by size:
2685 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3312 493
Size: 711 2108 2702
Fragments arranged by size:
2702 2108 711
BsaBI GATnn'nnATC
Cuts at: 5515 5515
Size: 5521
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2084 2332 2805 3328
Size: 53 83 186 936 551 248 473 523
Cuts at: 3328 3710 4345 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 635 551 523 473 382 248
186 83 53
BsaWI w'CCGG_w
Cuts at: 3954 4352 4626 4773 5184 3954
Size: 398 274 147 411 4291
Fragments arranged by size:
4291 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5296
Fragments arranged by size:
5296 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2093 2372 1186
Size: 904 3 279 4335
Fragments arranged by size:
4335 904 279 3
BseYI C'CCAG_C
Cuts at: 2680 2712 2814 4724 5220 2680
Size: 32 102 1910 496 2981
Fragments arranged by size:
2981 1910 496 102 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4948
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2070 2250 2274 2287 2971 3732
Size: 275 186 69 180 24 13 684 761
Cuts at: 3732 4760 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 684 275 186 180 69
24 13
BsiHKAI G_wGCw'C
Cuts at: 3582 3667 4738 3582
Size: 85 1071 4365
Fragments arranged by size:
4365 1071 85
BsiWI C'GTAC_G
Cuts at: 2725 2725
Size: 5521
BsmI GAATG_Cn'
Cuts at: 3032 3125 3032
Size: 93 5428
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2210 2577 2840 3582 3667 4738
Size: 399 369 51 367 263 742 85 1071
Cuts at: 4738 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 369 367 263 85
51
BspEI T'CCGG_A
Cuts at: 5184 5184
Size: 5521
BspHI T'CATG_A
Cuts at: 3410 5140 3410
Size: 1730 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2876 844
Size: 2032 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3408 1394
Size: 429 1585 3507
Fragments arranged by size:
3507 1585 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4016 4190 65
Size: 1183 2768 174 1396
Fragments arranged by size:
2768 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2328 2398 2630 4165 4352 1700
Size: 110 518 70 232 1535 187 2869
Fragments arranged by size:
2869 1535 518 232 187 110 70
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5521
BssHII G'CGCG_C
Cuts at: 1925 2126 2128 2425 2427 2620 1925
Size: 201 2 297 2 193 4826
Fragments arranged by size:
4826 297 201 193 2 2
BssSI C'ACGA_G
Cuts at: 3581 4593 3581
Size: 1012 4509
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5521
BstBI TT'CG_AA
Cuts at: 4325 4325
Size: 5521
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5521
BstYI r'GATC_y
Cuts at: 1972 2851 3218 3603 3620 5072 5158 5170 5516
Size: 879 367 385 17 1452 86 12 346
Cuts at: 5516 1972
Size: 1977
Fragments arranged by size:
1977 1452 879 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2029 2640 513
Size: 552 964 611 3394
Fragments arranged by size:
3394 964 611 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4782
Fragments arranged by size:
4782 683 56
BtsI GCAGTG_nn'
Cuts at: 3037 3831 3851 3037
Size: 794 20 4707
Fragments arranged by size:
4707 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5521
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5486
DraI TTT'AAA
Cuts at: 2825 2825
Size: 5521
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4528 809
Size: 507 3212 1802
Fragments arranged by size:
3212 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2247 2271
Size: 54 1425 50 275 18 237 180 24
Cuts at: 2271 2478 2511 2670 2968 3857 5486 8
Size: 207 33 159 298 889 1629 43
Fragments arranged by size:
1629 1425 889 298 275 237 207 180
159 54 50 43 33 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2067 2247 2271 2968 1537
Size: 275 255 180 24 697 4090
Fragments arranged by size:
4090 697 275 255 180 24
EarI CTCTTCn'nnn_
Cuts at: 1353 2610 3451 1353
Size: 1257 841 3423
Fragments arranged by size:
3423 1257 841
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2294 2732 4108 4482 4628 387
Size: 1064 138 705 438 1376 374 146 1280
Fragments arranged by size:
1376 1280 1064 705 438 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2058 2189 2414 2477 3584 4181 4968
Size: 393 426 131 225 63 1107 597 787
Cuts at: 4968 5236 1239
Size: 268 1524
Fragments arranged by size:
1524 1107 787 597 426 393 268 225
131 63
EcoO109I rG'GnC_Cy
Cuts at: 2266 2573 2266
Size: 307 5214
EcoRI G'AATT_C
Cuts at: 2830 3208 2830
Size: 378 5143
FspI TGC'GCA
Cuts at: 4027 4027
Size: 5521
HaeII r_GCGC'y
Cuts at: 1678 2087 2335 2375 2381 2393 2450 2648 2755
Size: 409 248 40 6 12 57 198 107
Cuts at: 2755 2808 4668 1678
Size: 53 1860 2531
Fragments arranged by size:
2531 1860 409 248 198 107 57 53
40 12 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2453 2485 4162 4194 4236
Size: 32 135 32 881 32 1677 32 42
Cuts at: 4236 4268 1373
Size: 32 2626
Fragments arranged by size:
2626 1677 881 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2865 3111 669
Size: 988 281 927 246 3079
Fragments arranged by size:
3079 988 927 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5521
HpaI GTT'AAC
Cuts at: 3111 3111
Size: 5521
KasI G'GCGC_C
Cuts at: 2083 2331 2804 2083
Size: 248 473 4800
Fragments arranged by size:
4800 473 248
KpnI G_GTAC'C
Cuts at: 2846 2846
Size: 5521
MfeI C'AATT_G
Cuts at: 3120 3120
Size: 5521
MluI A'CGCG_T
Cuts at: 5397 5397
Size: 5521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4635 4819 1305
Size: 3330 184 2007
Fragments arranged by size:
3330 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3699
Fragments arranged by size:
3699 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 2654 3479 3838 3997 518
Size: 648 1020 468 825 359 159 2042
Fragments arranged by size:
2042 1020 825 648 468 359 159
MspA1I CmG'CkG
Cuts at: 1606 2031 2059 2688 2937 3614 4762 5007 1606
Size: 425 28 629 249 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 629 425 249 245 28
NaeI GCC'GGC
Cuts at: 2330 2400 2632 2330
Size: 70 232 5219
Fragments arranged by size:
5219 232 70
NarI GG'CG_CC
Cuts at: 2084 2332 2805 2084
Size: 248 473 4800
Fragments arranged by size:
4800 473 248
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4969
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5521
NgoMIV G'CCGG_C
Cuts at: 2328 2398 2630 2328
Size: 70 232 5219
Fragments arranged by size:
5219 232 70
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5521
NotI GC'GGCC_GC
Cuts at: 2968 2968
Size: 5521
NspI r_CATG'y
Cuts at: 1132 1229 2879 4424 1132
Size: 97 1650 1545 2229
Fragments arranged by size:
2229 1650 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5521
PciI A'CATG_T
Cuts at: 4420 4420
Size: 5521
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5521
PfoI T'CCnGG_A
Cuts at: 2592 2592
Size: 5521
PmeI GTTT'AAAC
Cuts at: 2825 2825
Size: 5521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3622 5103 5135 3590
Size: 32 1481 32 3976
Fragments arranged by size:
3976 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5521
PsiI TTA'TAA
Cuts at: 3091 3091
Size: 5521
PspOMI G'GGCC_C
Cuts at: 2206 2573 2206
Size: 367 5154
PspXI vC'TCGA_Gb
Cuts at: 2435 2609 2738 2435
Size: 174 129 5218
Fragments arranged by size:
5218 174 129
PstI C_TGCA'G
Cuts at: 830 2385 2772 2873 830
Size: 1555 387 101 3478
Fragments arranged by size:
3478 1555 387 101
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5521
PvuII CAG'CTG
Cuts at: 2059 2059
Size: 5521
SacII CC_GC'GG
Cuts at: 2032 2032
Size: 5521
SalI G'TCGA_C
Cuts at: 1936 2863 1936
Size: 927 4594
SapI GCTCTTCn'nnn_
Cuts at: 2610 2610
Size: 5521
SbfI CC_TGCA'GG
Cuts at: 2873 2873
Size: 5521
ScaI AGT'ACT
Cuts at: 3769 5481 3769
Size: 1712 3809
SexAI A'CCwGG_T
Cuts at: 2538 2538
Size: 5521
SfcI C'TryA_G
Cuts at: 826 1045 2381 2768 2869 4004 4685 4876 826
Size: 219 1336 387 101 1135 681 191 1471
Fragments arranged by size:
1471 1336 1135 681 387 219 191 101
SfoI GGC'GCC
Cuts at: 2085 2333 2806 2085
Size: 248 473 4800
Fragments arranged by size:
4800 473 248
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
SgrAI Cr'CCGG_yG
Cuts at: 1700 2328 1700
Size: 628 4893
SmaI CCC'GGG
Cuts at: 1872 2346 2848 1872
Size: 474 502 4545
Fragments arranged by size:
4545 502 474
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2435 2609 2738 3625 4526 4788
Size: 197 926 492 174 129 887 901 262
Cuts at: 4788 820
Size: 1553
Fragments arranged by size:
1553 926 901 887 492 262 197 174
129
SnaBI TAC'GTA
Cuts at: 493 3312 493
Size: 2819 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5521
SphI G_CATG'C
Cuts at: 1132 2879 1132
Size: 1747 3774
SspI AAT'ATT
Cuts at: 5 52 3445 5
Size: 47 3393 2081
Fragments arranged by size:
3393 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5521
StyI C'CwwG_G
Cuts at: 513 1065 2151 2184 2960 513
Size: 552 1086 33 776 3074
Fragments arranged by size:
3074 1086 776 552 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3548 3565 3718 3903 4348 325
Size: 1255 1968 17 153 185 445 1498
Fragments arranged by size:
1968 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3767 5479 96
Size: 274 80 33 51 1443 1790 1712 138
Fragments arranged by size:
1790 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5481 5513 5481
Size: 32 5489
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5521
XbaI T'CTAG_A
Cuts at: 2857 2857
Size: 5521
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2590 2590
Size: 5521
XhoI C'TCGA_G
Cuts at: 1943 2435 2609 2738 1943
Size: 492 174 129 4726
Fragments arranged by size:
4726 492 174 129
XmaI C'CCGG_G
Cuts at: 1870 2344 2846 1870
Size: 474 502 4545
Fragments arranged by size:
4545 502 474
XmnI GAAnn'nnTTC
Cuts at: 3650 3650
Size: 5521
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3329 276
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PpuMI
PsrI RsrII SacI SanDI SfiI SrfI SwaI