Restriction map for
pFN21AB9811
(Circular) (Six-base) MAPSORT of: pFN21AB9811.seq Check: 1308 from: 1 to: 5449
pFN21AB9811 5449 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3259 278
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2792 1937
Size: 855 4594
Acc65I G'GTAC_C
Cuts at: 2770 2770
Size: 5449
AclI AA'CG_TT
Cuts at: 1563 3576 3949 1563
Size: 2013 373 3063
Fragments arranged by size:
3063 2013 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2387 3512 4896 5164 1632
Size: 755 1125 1384 268 1917
Fragments arranged by size:
1917 1384 1125 755 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5252
AflIII A'CryG_T
Cuts at: 1203 4348 5325 1203
Size: 3145 977 1327
Fragments arranged by size:
3145 1327 977
AgeI A'CCGG_T
Cuts at: 2411 4280 2411
Size: 1869 3580
AhdI GACnn_n'nnGTC
Cuts at: 4178 4178
Size: 5449
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5449
AlwNI CAG_nnn'CTG
Cuts at: 2671 3192 4764 2671
Size: 521 1572 3356
Fragments arranged by size:
3356 1572 521
ApaI G_GGCC'C
Cuts at: 2241 2241
Size: 5449
ApaLI G'TGCA_C
Cuts at: 3506 4662 3506
Size: 1156 4293
ApoI r'AATT_y
Cuts at: 1493 2758 2984 3136 5245 1493
Size: 1265 226 152 2109 1697
Fragments arranged by size:
2109 1697 1265 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4003 160
Size: 872 2971 1606
Fragments arranged by size:
2971 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
AvaI C'yCGr_G
Cuts at: 1870 1943 2240 2564 2774 1870
Size: 73 297 324 210 4545
Fragments arranged by size:
4545 324 297 210 73
BamHI G'GATC_C
Cuts at: 2779 3146 2779
Size: 367 5082
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2087 2770 4225 618
Size: 325 217 627 300 683 1455 1842
Fragments arranged by size:
1842 1455 683 627 325 300 217
BanII G_rGCy'C
Cuts at: 1391 2112 2225 2241 2280 2742 2768 1391
Size: 721 113 16 39 462 26 4072
Fragments arranged by size:
4072 721 462 113 39 26 16
BbeI G_GCGC'C
Cuts at: 2091 2091
Size: 5449
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2350 2530 928
Size: 971 451 180 3847
Fragments arranged by size:
3847 971 451 180
BbvCI CC'TCA_GC
Cuts at: 2627 2627
Size: 5449
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3640 3674 1903
Size: 34 1703 34 3678
Fragments arranged by size:
3678 1703 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3341 4551 3341
Size: 1210 4239
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5449
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 2405 4060
Size: 107 122 71 631 605 367 366 1655
Cuts at: 4060 136
Size: 1525
Fragments arranged by size:
1655 1525 631 605 367 366 122 107
71
BglII A'GATC_T
Cuts at: 5444 5444
Size: 5449
BlpI GC'TnA_GC
Cuts at: 2866 2866
Size: 5449
Bme1580I G_kGCm'C
Cuts at: 1790 2241 3510 4666 1790
Size: 451 1269 1156 2573
Fragments arranged by size:
2573 1269 1156 451
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5449
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3180 4138 442
Size: 416 53 83 399 24 1763 958 1753
Fragments arranged by size:
1763 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5449
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2128 2239 2593 2128
Size: 111 354 4984
Fragments arranged by size:
4984 354 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2200 2264 4109 1239
Size: 961 64 1845 2579
Fragments arranged by size:
2579 1845 961 64
Bpu10I CC'TnA_GC
Cuts at: 2627 2627
Size: 5449
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3574 4439 4737 3574
Size: 865 298 4286
Fragments arranged by size:
4286 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4112 882
Size: 617 2613 2219
Fragments arranged by size:
2613 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3240 493
Size: 711 2036 2702
Fragments arranged by size:
2702 2036 711
BsaBI GATnn'nnATC
Cuts at: 5443 5443
Size: 5449
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2088 2343 3256 3638
Size: 53 83 186 936 555 255 913 382
Cuts at: 3638 4273 275
Size: 635 1451
Fragments arranged by size:
1451 936 913 635 555 382 255 186
83 53
BsaWI w'CCGG_w
Cuts at: 2411 3882 4280 4554 4701 5112 2411
Size: 1471 398 274 147 411 2748
Fragments arranged by size:
2748 1471 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5224
Fragments arranged by size:
5224 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2231 2243 2582 1186
Size: 934 111 12 339 4053
Fragments arranged by size:
4053 934 339 111 12
BseYI C'CCAG_C
Cuts at: 2302 4652 5148 2302
Size: 2350 496 2603
Fragments arranged by size:
2603 2350 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2603 1163
Size: 573 867 4009
Fragments arranged by size:
4009 867 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2700 2899 3660 4688 1540
Size: 275 186 699 199 761 1028 2301
Fragments arranged by size:
2301 1028 761 699 275 199 186
BsiHKAI G_wGCw'C
Cuts at: 2112 2280 3510 3595 4666 2112
Size: 168 1230 85 1071 2895
Fragments arranged by size:
2895 1230 1071 168 85
BsmI GAATG_Cn'
Cuts at: 2960 3053 2960
Size: 93 5356
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2225 2241 2280 2742 2768 3510
Size: 399 322 113 16 39 462 26 742
Cuts at: 3510 3595 4666 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 462 399 322 113 85
39 26 16
BspEI T'CCGG_A
Cuts at: 5112 5112
Size: 5449
BspHI T'CATG_A
Cuts at: 2079 3338 5068 2079
Size: 1259 1730 2460
Fragments arranged by size:
2460 1730 1259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 2804 844
Size: 1295 665 3489
Fragments arranged by size:
3489 1295 665
BsrBI CCG'CTC
Cuts at: 1394 1823 3336 1394
Size: 429 1513 3507
Fragments arranged by size:
3507 1513 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3944 4118 65
Size: 1183 2696 174 1396
Fragments arranged by size:
2696 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2411 4093 4280 1700
Size: 110 601 1682 187 2869
Fragments arranged by size:
2869 1682 601 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5449
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5449
BssSI C'ACGA_G
Cuts at: 3509 4521 3509
Size: 1012 4437
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2671 794
Size: 1877 3572
BstBI TT'CG_AA
Cuts at: 4253 4253
Size: 5449
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5449
BstXI CCAn_nnnn'nTGG
Cuts at: 2512 2581 2512
Size: 69 5380
BstYI r'GATC_y
Cuts at: 1972 2779 3146 3531 3548 5000 5086 5098 5444
Size: 807 367 385 17 1452 86 12 346
Cuts at: 5444 1972
Size: 1977
Fragments arranged by size:
1977 1452 807 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2091 2466 2607 513
Size: 552 939 87 375 141 3355
Fragments arranged by size:
3355 939 552 375 141 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4710
Fragments arranged by size:
4710 683 56
BtsI GCAGTG_nn'
Cuts at: 2965 3759 3779 2965
Size: 794 20 4635
Fragments arranged by size:
4635 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5449
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5414
DraI TTT'AAA
Cuts at: 2753 2753
Size: 5449
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4456 809
Size: 507 3140 1802
Fragments arranged by size:
3140 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2164 2397 2406
Size: 54 1425 50 275 18 334 233 9
Cuts at: 2406 2583 2697 2896 3785 5414 8
Size: 177 114 199 889 1629 43
Fragments arranged by size:
1629 1425 889 334 275 233 199 177
114 54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2697 2896 1537
Size: 275 885 199 4090
Fragments arranged by size:
4090 885 275 199
EarI CTCTTCn'nnn_
Cuts at: 1353 2115 2709 3379 1353
Size: 762 594 670 3423
Fragments arranged by size:
3423 762 670 594
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2498 4036 4410 4556 387
Size: 1064 138 909 1538 374 146 1280
Fragments arranged by size:
1538 1280 1064 909 374 146 138
EcoICRI GAG'CTC
Cuts at: 2110 2278 2110
Size: 168 5281
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2200 2264 2387 3512 4109 4896 5164
Size: 393 568 64 123 1125 597 787 268
Cuts at: 5164 1239
Size: 1524
Fragments arranged by size:
1524 1125 787 597 568 393 268 123
64
EcoNI CCTnn'n_nnAGG
Cuts at: 2327 2399 2630 2327
Size: 72 231 5146
Fragments arranged by size:
5146 231 72
EcoO109I rG'GnC_Cy
Cuts at: 2237 2237
Size: 5449
EcoRI G'AATT_C
Cuts at: 2758 3136 2758
Size: 378 5071
FspI TGC'GCA
Cuts at: 3955 3955
Size: 5449
HaeII r_GCGC'y
Cuts at: 1678 2091 4596 1678
Size: 413 2505 2531
Fragments arranged by size:
2531 2505 413
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2002 2034 4090 4122 4164
Size: 32 135 32 430 32 2056 32 42
Cuts at: 4164 4196 1373
Size: 32 2626
Fragments arranged by size:
2626 2056 430 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2793 3039 669
Size: 988 281 855 246 3079
Fragments arranged by size:
3079 988 855 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5449
HpaI GTT'AAC
Cuts at: 3039 3039
Size: 5449
KasI G'GCGC_C
Cuts at: 2087 2087
Size: 5449
KpnI G_GTAC'C
Cuts at: 2774 2774
Size: 5449
MfeI C'AATT_G
Cuts at: 3048 3048
Size: 5449
MluI A'CGCG_T
Cuts at: 5325 5325
Size: 5449
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4563 4747 1305
Size: 3258 184 2007
Fragments arranged by size:
3258 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2408 2585 10
Size: 54 1425 343 576 177 2874
Fragments arranged by size:
2874 1425 576 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3407 3766 3925 518
Size: 648 2241 359 159 2042
Fragments arranged by size:
2241 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2668 2865 3542 4690 4935 1606
Size: 1062 197 677 1148 245 2120
Fragments arranged by size:
2120 1148 1062 677 245 197
NarI GG'CG_CC
Cuts at: 2088 2088
Size: 5449
NcoI C'CATG_G
Cuts at: 513 1065 2004 2466 2607 513
Size: 552 939 462 141 3355
Fragments arranged by size:
3355 939 552 462 141
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5449
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5449
NotI GC'GGCC_GC
Cuts at: 2896 2896
Size: 5449
NspI r_CATG'y
Cuts at: 1132 1229 2807 4352 1132
Size: 97 1578 1545 2229
Fragments arranged by size:
2229 1578 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5449
PciI A'CATG_T
Cuts at: 4348 4348
Size: 5449
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5449
PmeI GTTT'AAAC
Cuts at: 2753 2753
Size: 5449
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3550 5031 5063 3518
Size: 32 1481 32 3904
Fragments arranged by size:
3904 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5449
PsiI TTA'TAA
Cuts at: 3019 3019
Size: 5449
PspOMI G'GGCC_C
Cuts at: 2237 2237
Size: 5449
PstI C_TGCA'G
Cuts at: 830 2801 830
Size: 1971 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5449
PvuII CAG'CTG
Cuts at: 2668 2668
Size: 5449
SacI G_AGCT'C
Cuts at: 2112 2280 2112
Size: 168 5281
SalI G'TCGA_C
Cuts at: 1936 2791 1936
Size: 855 4594
SapI GCTCTTCn'nnn_
Cuts at: 2115 2115
Size: 5449
SbfI CC_TGCA'GG
Cuts at: 2801 2801
Size: 5449
ScaI AGT'ACT
Cuts at: 3697 5409 3697
Size: 1712 3737
SfcI C'TryA_G
Cuts at: 826 1045 2797 3932 4613 4804 826
Size: 219 1752 1135 681 191 1471
Fragments arranged by size:
1752 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2405 2405
Size: 5449
SfoI GGC'GCC
Cuts at: 2089 2089
Size: 5449
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
SgrAI Cr'CCGG_yG
Cuts at: 1700 2411 1700
Size: 711 4738
SmaI CCC'GGG
Cuts at: 1872 2776 1872
Size: 904 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3553 4454 4716 820
Size: 197 926 1610 901 262 1553
Fragments arranged by size:
1610 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3240 493
Size: 2747 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5449
SphI G_CATG'C
Cuts at: 1132 2807 1132
Size: 1675 3774
SspI AAT'ATT
Cuts at: 5 52 3373 5
Size: 47 3321 2081
Fragments arranged by size:
3321 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5449
StyI C'CwwG_G
Cuts at: 513 1065 2004 2466 2607 2888 513
Size: 552 939 462 141 281 3074
Fragments arranged by size:
3074 939 552 462 281 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3476 3493 3646 3831 4276 325
Size: 1255 1896 17 153 185 445 1498
Fragments arranged by size:
1896 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3695 5407 96
Size: 274 80 33 51 1443 1718 1712 138
Fragments arranged by size:
1718 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5409 5441 5409
Size: 32 5417
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5449
XbaI T'CTAG_A
Cuts at: 2785 2785
Size: 5449
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5449
XmaI C'CCGG_G
Cuts at: 1870 2774 1870
Size: 904 4545
XmnI GAAnn'nnTTC
Cuts at: 3578 3578
Size: 5449
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3257 276
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI
XcmI