Restriction map for
pFN21AB9809
(Circular) (Six-base) MAPSORT of: pFN21AB9809.seq Check: 6659 from: 1 to: 5428
pFN21AB9809 5428 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2415 2415
Size: 5428
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3238 278
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2771 1937
Size: 834 4594
Acc65I G'GTAC_C
Cuts at: 2749 2749
Size: 5428
AclI AA'CG_TT
Cuts at: 1563 3555 3928 1563
Size: 1992 373 3063
Fragments arranged by size:
3063 1992 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2223 2629 3491 4875 5143 1632
Size: 591 406 862 1384 268 1917
Fragments arranged by size:
1917 1384 862 591 406 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5231
AflIII A'CryG_T
Cuts at: 1203 4327 5304 1203
Size: 3124 977 1327
Fragments arranged by size:
3124 1327 977
AgeI A'CCGG_T
Cuts at: 4259 4259
Size: 5428
AhdI GACnn_n'nnGTC
Cuts at: 4157 4157
Size: 5428
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5428
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2672 1184
Size: 1488 3940
AlwNI CAG_nnn'CTG
Cuts at: 3171 4743 3171
Size: 1572 3856
ApaI G_GGCC'C
Cuts at: 2448 2448
Size: 5428
ApaLI G'TGCA_C
Cuts at: 3485 4641 3485
Size: 1156 4272
ApoI r'AATT_y
Cuts at: 1493 2737 2963 3115 5224 1493
Size: 1244 226 152 2109 1697
Fragments arranged by size:
2109 1697 1244 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3982 160
Size: 872 2950 1606
Fragments arranged by size:
2950 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
AvaI C'yCGr_G
Cuts at: 1870 1943 2753 1870
Size: 73 810 4545
Fragments arranged by size:
4545 810 73
BamHI G'GATC_C
Cuts at: 2758 3125 2758
Size: 367 5061
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2749 4204 618
Size: 325 217 627 962 1455 1842
Fragments arranged by size:
1842 1455 962 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2448 2747 1391
Size: 1057 299 4072
Fragments arranged by size:
4072 1057 299
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2075 2276 2598 928
Size: 971 176 201 322 3758
Fragments arranged by size:
3758 971 322 201 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3619 3653 1903
Size: 34 1682 34 3678
Fragments arranged by size:
3678 1682 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3320 4530 3320
Size: 1210 4218
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5428
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2482 4039
Size: 107 122 71 631 605 337 473 1557
Cuts at: 4039 136
Size: 1525
Fragments arranged by size:
1557 1525 631 605 473 337 122 107
71
BglII A'GATC_T
Cuts at: 5423 5423
Size: 5428
BlpI GC'TnA_GC
Cuts at: 2845 2845
Size: 5428
Bme1580I G_kGCm'C
Cuts at: 1790 2448 3489 4645 1790
Size: 658 1041 1156 2573
Fragments arranged by size:
2573 1156 1041 658
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5428
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2442 3159 4117
Size: 416 53 83 399 24 1025 717 958
Cuts at: 4117 442
Size: 1753
Fragments arranged by size:
1753 1025 958 717 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2595 2610 2717 4088 1239
Size: 1356 15 107 1371 2579
Fragments arranged by size:
2579 1371 1356 107 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3553 4418 4716 3553
Size: 865 298 4265
Fragments arranged by size:
4265 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2027 4091 882
Size: 617 528 2064 2219
Fragments arranged by size:
2219 2064 617 528
BsaAI yAC'GTr
Cuts at: 493 1204 3219 493
Size: 711 2015 2702
Fragments arranged by size:
2702 2015 711
BsaBI GATnn'nnATC
Cuts at: 5422 5422
Size: 5428
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2423 3235 3617 4252
Size: 53 83 186 936 890 812 382 635
Cuts at: 4252 275
Size: 1451
Fragments arranged by size:
1451 936 890 812 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2527 2555 3861 4259 4533 4680 5091 2527
Size: 28 1306 398 274 147 411 2864
Fragments arranged by size:
2864 1306 411 398 274 147 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2313 2343 1178
Size: 30 165 30 910 30 4263
Fragments arranged by size:
4263 910 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2645 1186
Size: 1459 3969
BseYI C'CCAG_C
Cuts at: 2080 2565 4631 5127 2080
Size: 485 2066 496 2381
Fragments arranged by size:
2381 2066 496 485
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4855
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2878 3639 4667 1540
Size: 275 186 877 761 1028 2301
Fragments arranged by size:
2301 1028 877 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2151 2417 2637 3489 3574 4645 2151
Size: 266 220 852 85 1071 2934
Fragments arranged by size:
2934 1071 852 266 220 85
BsmI GAATG_Cn'
Cuts at: 2325 2939 3032 2325
Size: 614 93 4721
Fragments arranged by size:
4721 614 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2417 2448 2637 2747 3489 3574
Size: 399 361 266 31 189 110 742 85
Cuts at: 3574 4645 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 361 266 189 110
85 31
BspEI T'CCGG_A
Cuts at: 2527 5091 2527
Size: 2564 2864
BspHI T'CATG_A
Cuts at: 3317 5047 3317
Size: 1730 3698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2415 2783 844
Size: 1571 368 3489
Fragments arranged by size:
3489 1571 368
BsrBI CCG'CTC
Cuts at: 1394 1823 3315 1394
Size: 429 1492 3507
Fragments arranged by size:
3507 1492 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3923 4097 65
Size: 1183 2675 174 1396
Fragments arranged by size:
2675 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2431 4072 4259 1700
Size: 110 621 1641 187 2869
Fragments arranged by size:
2869 1641 621 187 110
BsrGI T'GTAC_A
Cuts at: 96 2464 96
Size: 2368 3060
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5428
BssSI C'ACGA_G
Cuts at: 3488 4500 3488
Size: 1012 4416
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5428
BstBI TT'CG_AA
Cuts at: 4232 4232
Size: 5428
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5428
BstYI r'GATC_y
Cuts at: 1972 2193 2524 2758 3125 3510 3527 4979 5065
Size: 221 331 234 367 385 17 1452 86
Cuts at: 5065 5077 5423 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 331 234 221
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3937
Fragments arranged by size:
3937 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4689
Fragments arranged by size:
4689 683 56
BtsI GCAGTG_nn'
Cuts at: 2944 3738 3758 2944
Size: 794 20 4614
Fragments arranged by size:
4614 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5428
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5393
DraI TTT'AAA
Cuts at: 2732 2732
Size: 5428
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2278 4435 809
Size: 507 962 2157 1802
Fragments arranged by size:
2157 1802 962 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2371 2433 2875
Size: 54 1425 50 275 18 541 62 442
Cuts at: 2875 3764 5393 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 541 442 275 62 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2875 1537
Size: 275 1063 4090
Fragments arranged by size:
4090 1063 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2241 3358 1353
Size: 888 1117 3423
Fragments arranged by size:
3423 1117 888
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4015 4389 4535 387
Size: 1064 138 2426 374 146 1280
Fragments arranged by size:
2426 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2223 2595 2610 2629 2717 3491 4088
Size: 393 591 372 15 19 88 774 597
Cuts at: 4088 4875 5143 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 774 597 591 393 372 268
88 19 15
EcoO109I rG'GnC_Cy
Cuts at: 2444 2444
Size: 5428
EcoRI G'AATT_C
Cuts at: 2737 3115 2737
Size: 378 5050
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2188
Size: 5428
FspI TGC'GCA
Cuts at: 3934 3934
Size: 5428
HaeII r_GCGC'y
Cuts at: 1678 4575 1678
Size: 2897 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2313 2345 2516 2548 4069
Size: 32 135 32 741 32 171 32 1521
Cuts at: 4069 4101 4143 4175 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1521 741 171 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2772 3018 669
Size: 988 281 834 246 3079
Fragments arranged by size:
3079 988 834 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5428
HpaI GTT'AAC
Cuts at: 3018 3018
Size: 5428
KpnI G_GTAC'C
Cuts at: 2753 2753
Size: 5428
MfeI C'AATT_G
Cuts at: 3027 3027
Size: 5428
MluI A'CGCG_T
Cuts at: 5304 5304
Size: 5428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4542 4726 1305
Size: 3237 184 2007
Fragments arranged by size:
3237 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2373 10
Size: 54 1425 343 541 3065
Fragments arranged by size:
3065 1425 541 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3386 3745 3904 518
Size: 648 2220 359 159 2042
Fragments arranged by size:
2220 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2015 2844 3521 4669 4914 1606
Size: 409 829 677 1148 245 2120
Fragments arranged by size:
2120 1148 829 677 409 245
NaeI GCC'GGC
Cuts at: 2433 2433
Size: 5428
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3937
Fragments arranged by size:
3937 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5428
NgoMIV G'CCGG_C
Cuts at: 2431 2431
Size: 5428
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5428
NotI GC'GGCC_GC
Cuts at: 2875 2875
Size: 5428
NspI r_CATG'y
Cuts at: 1132 1229 2786 4331 1132
Size: 97 1557 1545 2229
Fragments arranged by size:
2229 1557 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5428
PciI A'CATG_T
Cuts at: 4327 4327
Size: 5428
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5428
PfoI T'CCnGG_A
Cuts at: 2331 2331
Size: 5428
PmeI GTTT'AAAC
Cuts at: 2732 2732
Size: 5428
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 5010 5042 3497
Size: 32 1481 32 3883
Fragments arranged by size:
3883 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5428
PsiI TTA'TAA
Cuts at: 2998 2998
Size: 5428
PspOMI G'GGCC_C
Cuts at: 2444 2444
Size: 5428
PstI C_TGCA'G
Cuts at: 830 2412 2780 830
Size: 1582 368 3478
Fragments arranged by size:
3478 1582 368
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5428
SalI G'TCGA_C
Cuts at: 1936 2770 1936
Size: 834 4594
SbfI CC_TGCA'GG
Cuts at: 2780 2780
Size: 5428
ScaI AGT'ACT
Cuts at: 3676 5388 3676
Size: 1712 3716
SfcI C'TryA_G
Cuts at: 826 1045 2408 2776 3911 4592 4783 826
Size: 219 1363 368 1135 681 191 1471
Fragments arranged by size:
1471 1363 1135 681 368 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5428
SmaI CCC'GGG
Cuts at: 1872 2755 1872
Size: 883 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3532 4433 4695 820
Size: 197 926 1589 901 262 1553
Fragments arranged by size:
1589 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3219 493
Size: 2726 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5428
SphI G_CATG'C
Cuts at: 1132 2786 1132
Size: 1654 3774
SspI AAT'ATT
Cuts at: 5 52 3352 5
Size: 47 3300 2081
Fragments arranged by size:
3300 2081 47
StuI AGG'CCT
Cuts at: 1517 2231 1517
Size: 714 4714
StyI C'CwwG_G
Cuts at: 513 1065 2004 2867 513
Size: 552 939 863 3074
Fragments arranged by size:
3074 939 863 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2417 3455 3472 3625 3810 4255 325
Size: 1255 837 1038 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1038 837 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2464 2677 3674
Size: 274 80 33 51 1443 487 213 997
Cuts at: 3674 5386 96
Size: 1712 138
Fragments arranged by size:
1712 1443 997 487 274 213 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5388 5420 5388
Size: 32 5396
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5428
XbaI T'CTAG_A
Cuts at: 2764 2764
Size: 5428
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5428
XmaI C'CCGG_G
Cuts at: 1870 2753 1870
Size: 883 4545
XmnI GAAnn'nnTTC
Cuts at: 3557 3557
Size: 5428
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3236 276
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI