Restriction map for
pFN21AB9804
(Circular) (Six-base) MAPSORT of: pFN21AB9804.seq Check: 6963 from: 1 to: 5374
pFN21AB9804 5374 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3184 278
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2717 1937
Size: 780 4594
Acc65I G'GTAC_C
Cuts at: 2695 2695
Size: 5374
AclI AA'CG_TT
Cuts at: 1563 3501 3874 1563
Size: 1938 373 3063
Fragments arranged by size:
3063 1938 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3437 4821 5089 1632
Size: 1805 1384 268 1917
Fragments arranged by size:
1917 1805 1384 268
AfeI AGC'GCT
Cuts at: 2167 2167
Size: 5374
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5177
AflIII A'CryG_T
Cuts at: 1203 2671 4273 5250 1203
Size: 1468 1602 977 1327
Fragments arranged by size:
1602 1468 1327 977
AgeI A'CCGG_T
Cuts at: 4205 4205
Size: 5374
AhdI GACnn_n'nnGTC
Cuts at: 4103 4103
Size: 5374
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5374
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5374
AlwNI CAG_nnn'CTG
Cuts at: 3117 4689 3117
Size: 1572 3802
ApaI G_GGCC'C
Cuts at: 2313 2515 2313
Size: 202 5172
ApaLI G'TGCA_C
Cuts at: 3431 4587 3431
Size: 1156 4218
ApoI r'AATT_y
Cuts at: 1493 2545 2683 2909 3061 5170 1493
Size: 1052 138 226 152 2109 1697
Fragments arranged by size:
2109 1697 1052 226 152 138
AseI AT'TA_AT
Cuts at: 160 1032 3928 160
Size: 872 2896 1606
Fragments arranged by size:
2896 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
AvaI C'yCGr_G
Cuts at: 1870 1943 2516 2699 1870
Size: 73 573 183 4545
Fragments arranged by size:
4545 573 183 73
BamHI G'GATC_C
Cuts at: 2704 3071 2704
Size: 367 5007
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2421 2579 2695 4150 618
Size: 325 217 627 634 158 116 1455 1842
Fragments arranged by size:
1842 1455 634 627 325 217 158 116
BanII G_rGCy'C
Cuts at: 1391 2264 2313 2515 2693 1391
Size: 873 49 202 178 4072
Fragments arranged by size:
4072 873 202 178 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2306 928
Size: 971 407 3996
Fragments arranged by size:
3996 971 407
BbvCI CC'TCA_GC
Cuts at: 2507 2507
Size: 5374
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2141 2175 3565 3599 1903
Size: 34 50 34 120 34 1390 34 3678
Fragments arranged by size:
3678 1390 120 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3266 4476 3266
Size: 1210 4164
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5374
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2375 2384 3985
Size: 107 122 71 631 605 703 9 1601
Cuts at: 3985 136
Size: 1525
Fragments arranged by size:
1601 1525 703 631 605 122 107 71
9
BglII A'GATC_T
Cuts at: 5369 5369
Size: 5374
BlpI GC'TnA_GC
Cuts at: 2791 2791
Size: 5374
Bme1580I G_kGCm'C
Cuts at: 1790 2313 2515 3435 4591 1790
Size: 523 202 920 1156 2573
Fragments arranged by size:
2573 1156 920 523 202
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5374
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3105 4063 442
Size: 416 53 83 399 24 1688 958 1753
Fragments arranged by size:
1753 1688 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5374
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2230 2230
Size: 5374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4034 1239
Size: 2795 2579
Bpu10I CC'TnA_GC
Cuts at: 2507 2507
Size: 5374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3499 4364 4662 3499
Size: 865 298 4211
Fragments arranged by size:
4211 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2225 4037 882
Size: 617 726 1812 2219
Fragments arranged by size:
2219 1812 726 617
BsaAI yAC'GTr
Cuts at: 493 1204 2373 3165 493
Size: 711 1169 792 2702
Fragments arranged by size:
2702 1169 792 711
BsaBI GATnn'nnATC
Cuts at: 5368 5368
Size: 5374
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3181 3563 4198 275
Size: 53 83 186 936 1648 382 635 1451
Fragments arranged by size:
1648 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3807 4205 4479 4626 5037 3807
Size: 398 274 147 411 4144
Fragments arranged by size:
4144 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5149
Fragments arranged by size:
5149 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2041 2516 2534 1186
Size: 855 475 18 4026
Fragments arranged by size:
4026 855 475 18
BseYI C'CCAG_C
Cuts at: 2556 4577 5073 2556
Size: 2021 496 2857
Fragments arranged by size:
2857 2021 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2255 2377 1163
Size: 573 519 122 4160
Fragments arranged by size:
4160 573 519 122
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2824 3585 4613 1540
Size: 275 186 823 761 1028 2301
Fragments arranged by size:
2301 1028 823 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3435 3520 4591 3435
Size: 85 1071 4218
Fragments arranged by size:
4218 1071 85
BsmI GAATG_Cn'
Cuts at: 2885 2978 2885
Size: 93 5281
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2264 2313 2515 2693 3435 3520 4591
Size: 399 474 49 202 178 742 85 1071
Cuts at: 4591 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 474 399 202 178 85
49
BspEI T'CCGG_A
Cuts at: 5037 5037
Size: 5374
BspHI T'CATG_A
Cuts at: 3263 4993 3263
Size: 1730 3644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2729 844
Size: 1885 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2267 3261 1394
Size: 429 444 994 3507
Fragments arranged by size:
3507 994 444 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2182 3869 4043 65
Size: 1183 934 1687 174 1396
Fragments arranged by size:
1687 1396 1183 934 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4018 4205 1700
Size: 110 2208 187 2869
Fragments arranged by size:
2869 2208 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5374
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5374
BssSI C'ACGA_G
Cuts at: 2242 3434 4446 2242
Size: 1192 1012 3170
Fragments arranged by size:
3170 1192 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5374
BstBI TT'CG_AA
Cuts at: 4178 4178
Size: 5374
BstEII G'GTnAC_C
Cuts at: 1184 2471 1184
Size: 1287 4087
BstXI CCAn_nnnn'nTGG
Cuts at: 2342 2342
Size: 5374
BstYI r'GATC_y
Cuts at: 1972 2328 2704 3071 3456 3473 4925 5011 5023
Size: 356 376 367 385 17 1452 86 12
Cuts at: 5023 5369 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 376 367 356 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2217 2626 513
Size: 552 1152 409 3261
Fragments arranged by size:
3261 1152 552 409
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4635
Fragments arranged by size:
4635 683 56
BtsI GCAGTG_nn'
Cuts at: 2890 3684 3704 2890
Size: 794 20 4560
Fragments arranged by size:
4560 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5374
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5339
DraI TTT'AAA
Cuts at: 2678 2678
Size: 5374
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4381 809
Size: 507 3065 1802
Fragments arranged by size:
3065 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2605 2629 2821
Size: 54 1425 50 275 18 775 24 192
Cuts at: 2821 3710 5339 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 775 275 192 54 50
43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2821 1537
Size: 275 1009 4090
Fragments arranged by size:
4090 1009 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2130 2199 3304 1353
Size: 777 69 1105 3423
Fragments arranged by size:
3423 1105 777 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3961 4335 4481 387
Size: 1064 138 2372 374 146 1280
Fragments arranged by size:
2372 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3437 4034 4821 5089 1239
Size: 393 1805 597 787 268 1524
Fragments arranged by size:
1805 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2519 2519
Size: 5374
EcoO109I rG'GnC_Cy
Cuts at: 2310 2511 2512 2310
Size: 201 1 5172
Fragments arranged by size:
5172 201 1
EcoRI G'AATT_C
Cuts at: 2683 3061 2683
Size: 378 4996
FspI TGC'GCA
Cuts at: 3880 3880
Size: 5374
HaeII r_GCGC'y
Cuts at: 1678 2169 4521 1678
Size: 491 2352 2531
Fragments arranged by size:
2531 2352 491
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 4015 4047 4089
Size: 32 135 32 467 32 1944 32 42
Cuts at: 4089 4121 1373
Size: 32 2626
Fragments arranged by size:
2626 1944 467 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2718 2964 669
Size: 988 281 780 246 3079
Fragments arranged by size:
3079 988 780 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5374
HpaI GTT'AAC
Cuts at: 2964 2964
Size: 5374
KpnI G_GTAC'C
Cuts at: 2699 2699
Size: 5374
MfeI C'AATT_G
Cuts at: 2973 2973
Size: 5374
MluI A'CGCG_T
Cuts at: 5250 5250
Size: 5374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4488 4672 1305
Size: 3183 184 2007
Fragments arranged by size:
3183 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2607 2631 10
Size: 54 1425 343 775 24 2753
Fragments arranged by size:
2753 1425 775 343 54 24
MslI CAynn'nnrTG
Cuts at: 518 1166 3332 3691 3850 518
Size: 648 2166 359 159 2042
Fragments arranged by size:
2166 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2381 2446 2560 2790 3467 4615 4860 1606
Size: 775 65 114 230 677 1148 245 2120
Fragments arranged by size:
2120 1148 775 677 245 230 114 65
NcoI C'CATG_G
Cuts at: 513 1065 2626 513
Size: 552 1561 3261
Fragments arranged by size:
3261 1561 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5374
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5374
NotI GC'GGCC_GC
Cuts at: 2821 2821
Size: 5374
NspI r_CATG'y
Cuts at: 1132 1229 2732 4277 1132
Size: 97 1503 1545 2229
Fragments arranged by size:
2229 1545 1503 97
PasI CC'CwG_GG
Cuts at: 1348 2413 1348
Size: 1065 4309
PciI A'CATG_T
Cuts at: 4273 4273
Size: 5374
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4472
PmeI GTTT'AAAC
Cuts at: 2678 2678
Size: 5374
PmlI CAC'GTG
Cuts at: 2373 2373
Size: 5374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 4956 4988 3443
Size: 32 1481 32 3829
Fragments arranged by size:
3829 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5374
PsiI TTA'TAA
Cuts at: 2944 2944
Size: 5374
PspOMI G'GGCC_C
Cuts at: 2309 2511 2309
Size: 202 5172
PspXI vC'TCGA_Gb
Cuts at: 2516 2516
Size: 5374
PstI C_TGCA'G
Cuts at: 830 2445 2726 830
Size: 1615 281 3478
Fragments arranged by size:
3478 1615 281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5374
SalI G'TCGA_C
Cuts at: 1936 2716 1936
Size: 780 4594
SbfI CC_TGCA'GG
Cuts at: 2726 2726
Size: 5374
ScaI AGT'ACT
Cuts at: 3622 5334 3622
Size: 1712 3662
SfcI C'TryA_G
Cuts at: 826 1045 2441 2722 3857 4538 4729 826
Size: 219 1396 281 1135 681 191 1471
Fragments arranged by size:
1471 1396 1135 681 281 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2375 2375
Size: 5374
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5374
SmaI CCC'GGG
Cuts at: 1872 2701 1872
Size: 829 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2516 3478 4379 4641 820
Size: 197 926 573 962 901 262 1553
Fragments arranged by size:
1553 962 926 901 573 262 197
SnaBI TAC'GTA
Cuts at: 493 3165 493
Size: 2672 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5374
SphI G_CATG'C
Cuts at: 1132 2732 1132
Size: 1600 3774
SspI AAT'ATT
Cuts at: 5 52 3298 5
Size: 47 3246 2081
Fragments arranged by size:
3246 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5374
StyI C'CwwG_G
Cuts at: 513 1065 2397 2484 2626 2813 513
Size: 552 1332 87 142 187 3074
Fragments arranged by size:
3074 1332 552 187 142 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2426 2570 2649 3401 3418 3571 3756
Size: 1255 846 144 79 752 17 153 185
Cuts at: 3756 4201 325
Size: 445 1498
Fragments arranged by size:
1498 1255 846 752 445 185 153 144
79 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3620 5332 96
Size: 274 80 33 51 1443 1643 1712 138
Fragments arranged by size:
1712 1643 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2264 2296 2465 2497 5334 5366 2264
Size: 32 169 32 2837 32 2272
Fragments arranged by size:
2837 2272 169 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5374
XbaI T'CTAG_A
Cuts at: 2710 2710
Size: 5374
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2192 2339 2624 2192
Size: 147 285 4942
Fragments arranged by size:
4942 285 147
XhoI C'TCGA_G
Cuts at: 1943 2516 1943
Size: 573 4801
XmaI C'CCGG_G
Cuts at: 1870 2699 1870
Size: 829 4545
XmnI GAAnn'nnTTC
Cuts at: 3503 3503
Size: 5374
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3182 276
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PstI PvuI SalI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PpuMI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfoI SrfI SwaI