Restriction map for
pFN21AB9789
(Circular) (Six-base) MAPSORT of: pFN21AB9789.seq Check: 1226 from: 1 to: 5311
pFN21AB9789 5311 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3121 278
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2654 1937
Size: 717 4594
Acc65I G'GTAC_C
Cuts at: 2632 2632
Size: 5311
AclI AA'CG_TT
Cuts at: 1563 3438 3811 1563
Size: 1875 373 3063
Fragments arranged by size:
3063 1875 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3374 4758 5026 1632
Size: 1742 1384 268 1917
Fragments arranged by size:
1917 1742 1384 268
AfeI AGC'GCT
Cuts at: 2412 2412
Size: 5311
AflII C'TTAA_G
Cuts at: 820 1017 2414 820
Size: 197 1397 3717
Fragments arranged by size:
3717 1397 197
AflIII A'CryG_T
Cuts at: 1203 4210 5187 1203
Size: 3007 977 1327
Fragments arranged by size:
3007 1327 977
AgeI A'CCGG_T
Cuts at: 4142 4142
Size: 5311
AhdI GACnn_n'nnGTC
Cuts at: 4040 4040
Size: 5311
AleI CACnn'nnGTG
Cuts at: 1166 2293 1166
Size: 1127 4184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5311
AlwNI CAG_nnn'CTG
Cuts at: 2362 3054 4626 2362
Size: 692 1572 3047
Fragments arranged by size:
3047 1572 692
ApaI G_GGCC'C
Cuts at: 2453 2529 2453
Size: 76 5235
ApaLI G'TGCA_C
Cuts at: 3368 4524 3368
Size: 1156 4155
ApoI r'AATT_y
Cuts at: 1493 2620 2846 2998 5107 1493
Size: 1127 226 152 2109 1697
Fragments arranged by size:
2109 1697 1127 226 152
AscI GG'CGCG_CC
Cuts at: 2105 2281 2105
Size: 176 5135
AseI AT'TA_AT
Cuts at: 160 1032 3865 160
Size: 872 2833 1606
Fragments arranged by size:
2833 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
AvaI C'yCGr_G
Cuts at: 1870 1943 2322 2501 2636 1870
Size: 73 379 179 135 4545
Fragments arranged by size:
4545 379 179 135 73
AvrII C'CTAG_G
Cuts at: 2536 2536
Size: 5311
BamHI G'GATC_C
Cuts at: 2641 3008 2641
Size: 367 4944
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2082 2471 2632 4087 618
Size: 325 217 627 295 389 161 1455 1842
Fragments arranged by size:
1842 1455 627 389 325 295 217 161
BanII G_rGCy'C
Cuts at: 1391 2193 2424 2453 2529 2630 1391
Size: 802 231 29 76 101 4072
Fragments arranged by size:
4072 802 231 101 76 29
BbeI G_GCGC'C
Cuts at: 2086 2475 2086
Size: 389 4922
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3502 3536 1903
Size: 34 1565 34 3678
Fragments arranged by size:
3678 1565 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3203 4413 3203
Size: 1210 4101
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5311
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2175 2457
Size: 107 122 71 631 605 337 166 282
Cuts at: 2457 2469 3922 136
Size: 12 1453 1525
Fragments arranged by size:
1525 1453 631 605 337 282 166 122
107 71 12
BglII A'GATC_T
Cuts at: 5306 5306
Size: 5311
BlpI GC'TnA_GC
Cuts at: 2728 2728
Size: 5311
Bme1580I G_kGCm'C
Cuts at: 1790 2453 2529 3372 4528 1790
Size: 663 76 843 1156 2573
Fragments arranged by size:
2573 1156 843 663 76
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5311
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3042 4000 442
Size: 416 53 83 399 24 1625 958 1753
Fragments arranged by size:
1753 1625 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 2393 2536 3971 1239
Size: 809 345 143 1435 2579
Fragments arranged by size:
2579 1435 809 345 143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2177 3436 4301 4599 2177
Size: 1259 865 298 2889
Fragments arranged by size:
2889 1259 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3974 882
Size: 617 2475 2219
Fragments arranged by size:
2475 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3102 493
Size: 711 1898 2702
Fragments arranged by size:
2702 1898 711
BsaBI GATnn'nnATC
Cuts at: 5305 5305
Size: 5311
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2083 2209 2472 3118
Size: 53 83 186 936 550 126 263 646
Cuts at: 3118 3500 4135 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 646 635 550 382 263 186
126 83 53
BsaWI w'CCGG_w
Cuts at: 2575 3744 4142 4416 4563 4974 2575
Size: 1169 398 274 147 411 2912
Fragments arranged by size:
2912 1169 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5086
Fragments arranged by size:
5086 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2060 2111 2402 1186
Size: 859 15 51 291 4095
Fragments arranged by size:
4095 859 291 51 15
BseYI C'CCAG_C
Cuts at: 2434 2465 4514 5010 2434
Size: 31 2049 496 2735
Fragments arranged by size:
2735 2049 496 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2144 1163
Size: 573 408 4330
Fragments arranged by size:
4330 573 408
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2134 2761 3522 4550 1540
Size: 275 186 133 627 761 1028 2301
Fragments arranged by size:
2301 1028 761 627 275 186 133
BsiHKAI G_wGCw'C
Cuts at: 2193 2424 3372 3457 4528 2193
Size: 231 948 85 1071 2976
Fragments arranged by size:
2976 1071 948 231 85
BsmI GAATG_Cn'
Cuts at: 2822 2915 2822
Size: 93 5218
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2193 2424 2453 2529 2630 3372 3457
Size: 399 403 231 29 76 101 742 85
Cuts at: 3457 4528 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 403 399 231 101 85
76 29
BspEI T'CCGG_A
Cuts at: 2575 4974 2575
Size: 2399 2912
BspHI T'CATG_A
Cuts at: 3200 4930 3200
Size: 1730 3581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2666 844
Size: 1822 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3198 1394
Size: 429 1375 3507
Fragments arranged by size:
3507 1375 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3806 3980 65
Size: 1183 2558 174 1396
Fragments arranged by size:
2558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2205 3955 4142 1700
Size: 110 395 1750 187 2869
Fragments arranged by size:
2869 1750 395 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5311
BssHII G'CGCG_C
Cuts at: 1925 2105 2281 1925
Size: 180 176 4955
Fragments arranged by size:
4955 180 176
BssSI C'ACGA_G
Cuts at: 3371 4383 3371
Size: 1012 4299
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5311
BstBI TT'CG_AA
Cuts at: 4115 4115
Size: 5311
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5311
BstYI r'GATC_y
Cuts at: 1972 2181 2641 3008 3393 3410 4862 4948 4960
Size: 209 460 367 385 17 1452 86 12
Cuts at: 4960 5306 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 460 385 367 346 209 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2455 513
Size: 552 939 451 3369
Fragments arranged by size:
3369 939 552 451
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2556 487
Size: 683 56 1330 3242
Fragments arranged by size:
3242 1330 683 56
BtsI GCAGTG_nn'
Cuts at: 2827 3621 3641 2827
Size: 794 20 4497
Fragments arranged by size:
4497 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5276
DraI TTT'AAA
Cuts at: 2615 2615
Size: 5311
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4318 809
Size: 507 3002 1802
Fragments arranged by size:
3002 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2131 2364 2758
Size: 54 1425 50 275 18 301 233 394
Cuts at: 2758 3647 5276 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 394 301 275 233 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2131 2758 1537
Size: 275 319 627 4090
Fragments arranged by size:
4090 627 319 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2064 2309 3241 1353
Size: 711 245 932 3423
Fragments arranged by size:
3423 932 711 245
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3898 4272 4418 387
Size: 1064 138 2309 374 146 1280
Fragments arranged by size:
2309 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2191 2422 2191
Size: 231 5080
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2393 2536 3374 3971 4758 5026
Size: 393 416 345 143 838 597 787 268
Cuts at: 5026 1239
Size: 1524
Fragments arranged by size:
1524 838 787 597 416 393 345 268
143
EcoNI CCTnn'n_nnAGG
Cuts at: 2141 2141
Size: 5311
EcoO109I rG'GnC_Cy
Cuts at: 2310 2338 2449 2450 2526 2310
Size: 28 111 1 76 5095
Fragments arranged by size:
5095 111 76 28 1
EcoRI G'AATT_C
Cuts at: 2620 2998 2620
Size: 378 4933
FspI TGC'GCA
Cuts at: 3817 3817
Size: 5311
HaeII r_GCGC'y
Cuts at: 1678 2060 2086 2269 2414 2435 2475 4458 1678
Size: 382 26 183 145 21 40 1983 2531
Fragments arranged by size:
2531 1983 382 183 145 40 26 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2534 2566 3952 3984 4026
Size: 32 135 32 962 32 1386 32 42
Cuts at: 4026 4058 1373
Size: 32 2626
Fragments arranged by size:
2626 1386 962 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2655 2901 669
Size: 988 281 717 246 3079
Fragments arranged by size:
3079 988 717 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5311
HpaI GTT'AAC
Cuts at: 2901 2901
Size: 5311
KasI G'GCGC_C
Cuts at: 2082 2471 2082
Size: 389 4922
KpnI G_GTAC'C
Cuts at: 2636 2636
Size: 5311
MfeI C'AATT_G
Cuts at: 2910 2910
Size: 5311
MluI A'CGCG_T
Cuts at: 5187 5187
Size: 5311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4425 4609 1305
Size: 3120 184 2007
Fragments arranged by size:
3120 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3489
Fragments arranged by size:
3489 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2293 3269 3628 3787 518
Size: 648 1127 976 359 159 2042
Fragments arranged by size:
2042 1127 976 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2161 2457 2469 2727 3404 4552 4797 1606
Size: 555 296 12 258 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 555 296 258 245 12
NarI GG'CG_CC
Cuts at: 2083 2472 2083
Size: 389 4922
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3820
Fragments arranged by size:
3820 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5311
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5311
NotI GC'GGCC_GC
Cuts at: 2131 2758 2131
Size: 627 4684
NspI r_CATG'y
Cuts at: 1132 1229 2669 4214 1132
Size: 97 1440 1545 2229
Fragments arranged by size:
2229 1545 1440 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5311
PciI A'CATG_T
Cuts at: 4210 4210
Size: 5311
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5311
PfoI T'CCnGG_A
Cuts at: 2253 2253
Size: 5311
PmeI GTTT'AAAC
Cuts at: 2615 2615
Size: 5311
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 4893 4925 3380
Size: 32 1481 32 3766
Fragments arranged by size:
3766 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2310 2338 2310
Size: 28 5283
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5311
PsiI TTA'TAA
Cuts at: 2881 2881
Size: 5311
PspOMI G'GGCC_C
Cuts at: 2449 2525 2449
Size: 76 5235
PstI C_TGCA'G
Cuts at: 830 2166 2217 2663 830
Size: 1336 51 446 3478
Fragments arranged by size:
3478 1336 446 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5311
PvuII CAG'CTG
Cuts at: 2161 2161
Size: 5311
SacI G_AGCT'C
Cuts at: 2193 2424 2193
Size: 231 5080
SacII CC_GC'GG
Cuts at: 2458 2458
Size: 5311
SalI G'TCGA_C
Cuts at: 1936 2653 1936
Size: 717 4594
SanDI GG'GwC_CC
Cuts at: 2310 2310
Size: 5311
SapI GCTCTTCn'nnn_
Cuts at: 2064 2309 2064
Size: 245 5066
SbfI CC_TGCA'GG
Cuts at: 2663 2663
Size: 5311
ScaI AGT'ACT
Cuts at: 3559 5271 3559
Size: 1712 3599
SfcI C'TryA_G
Cuts at: 826 1045 2162 2213 2659 3794 4475 4666 826
Size: 219 1117 51 446 1135 681 191 1471
Fragments arranged by size:
1471 1135 1117 681 446 219 191 51
SfoI GGC'GCC
Cuts at: 2084 2473 2084
Size: 389 4922
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5311
SmaI CCC'GGG
Cuts at: 1872 2324 2638 1872
Size: 452 314 4545
Fragments arranged by size:
4545 452 314
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2192 2414 3415 4316 4578 820
Size: 197 926 249 222 1001 901 262 1553
Fragments arranged by size:
1553 1001 926 901 262 249 222 197
SnaBI TAC'GTA
Cuts at: 493 3102 493
Size: 2609 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5311
SphI G_CATG'C
Cuts at: 1132 2669 1132
Size: 1537 3774
SspI AAT'ATT
Cuts at: 5 52 3235 5
Size: 47 3183 2081
Fragments arranged by size:
3183 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5311
StyI C'CwwG_G
Cuts at: 513 1065 2004 2536 2750 513
Size: 552 939 532 214 3074
Fragments arranged by size:
3074 939 552 532 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3338 3355 3508 3693 4138 325
Size: 1255 1758 17 153 185 445 1498
Fragments arranged by size:
1758 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3557 5269 96
Size: 274 80 33 51 1443 1580 1712 138
Fragments arranged by size:
1712 1580 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5271 5303 5271
Size: 32 5279
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5311
XbaI T'CTAG_A
Cuts at: 2647 2647
Size: 5311
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5311
XmaI C'CCGG_G
Cuts at: 1870 2322 2636 1870
Size: 452 314 4545
Fragments arranged by size:
4545 452 314
XmnI GAAnn'nnTTC
Cuts at: 3440 3440
Size: 5311
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3119 276
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI BaeI BbvCI BfrBI BplI Bpu10I BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SexAI SfiI SrfI SwaI XcmI