Restriction map for
pFN21AB9784
(Circular) (Six-base) MAPSORT of: pFN21AB9784.seq Check: 2222 from: 1 to: 5272
pFN21AB9784 5272 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2467 2535 2467
Size: 68 5204
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3082 278
Size: 53 83 186 936 1546 2468
Fragments arranged by size:
2468 1546 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2615 1937
Size: 678 4594
Acc65I G'GTAC_C
Cuts at: 2593 2593
Size: 5272
AclI AA'CG_TT
Cuts at: 1563 3399 3772 1563
Size: 1836 373 3063
Fragments arranged by size:
3063 1836 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3335 4719 4987 1632
Size: 1703 1384 268 1917
Fragments arranged by size:
1917 1703 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5075
AflIII A'CryG_T
Cuts at: 1203 4171 5148 1203
Size: 2968 977 1327
Fragments arranged by size:
2968 1327 977
AgeI A'CCGG_T
Cuts at: 4103 4103
Size: 5272
AhdI GACnn_n'nnGTC
Cuts at: 4001 4001
Size: 5272
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5272
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5272
AlwNI CAG_nnn'CTG
Cuts at: 2296 3015 4587 2296
Size: 719 1572 2981
Fragments arranged by size:
2981 1572 719
ApaI G_GGCC'C
Cuts at: 2041 2202 2444 2041
Size: 161 242 4869
Fragments arranged by size:
4869 242 161
ApaLI G'TGCA_C
Cuts at: 3329 4485 3329
Size: 1156 4116
ApoI r'AATT_y
Cuts at: 1493 2581 2807 2959 5068 1493
Size: 1088 226 152 2109 1697
Fragments arranged by size:
2109 1697 1088 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3826 160
Size: 872 2794 1606
Fragments arranged by size:
2794 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5272
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 2101 2142 2597 1870
Size: 73 123 35 41 455 4545
Fragments arranged by size:
4545 455 123 73 41 35
BamHI G'GATC_C
Cuts at: 2602 2969 2602
Size: 367 4905
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2031 2567 2593 4048 618
Size: 325 217 627 244 536 26 1455 1842
Fragments arranged by size:
1842 1455 627 536 325 244 217 26
BanII G_rGCy'C
Cuts at: 1391 2041 2161 2202 2444 2591 1391
Size: 650 120 41 242 147 4072
Fragments arranged by size:
4072 650 242 147 120 41
BbeI G_GCGC'C
Cuts at: 2035 2035
Size: 5272
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2015 928
Size: 971 116 4185
Fragments arranged by size:
4185 971 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3463 3497 1903
Size: 34 1526 34 3678
Fragments arranged by size:
3678 1526 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3164 4374 3164
Size: 1210 4062
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5272
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2029 2291 2424
Size: 107 122 71 631 605 357 262 133
Cuts at: 2424 2507 3883 136
Size: 83 1376 1525
Fragments arranged by size:
1525 1376 631 605 357 262 133 122
107 83 71
BglII A'GATC_T
Cuts at: 5267 5267
Size: 5272
BlpI GC'TnA_GC
Cuts at: 2689 2689
Size: 5272
Bme1580I G_kGCm'C
Cuts at: 1790 2041 2202 2444 2570 3333 4489 1790
Size: 251 161 242 126 763 1156 2573
Fragments arranged by size:
2573 1156 763 251 242 161 126
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5272
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3003 3961 442
Size: 416 53 83 399 24 1586 958 1753
Fragments arranged by size:
1753 1586 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5272
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2258 2450 3932 1239
Size: 1019 192 1482 2579
Fragments arranged by size:
2579 1482 1019 192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3397 4262 4560 3397
Size: 865 298 4109
Fragments arranged by size:
4109 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3935 882
Size: 617 2436 2219
Fragments arranged by size:
2436 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2317 3063 493
Size: 711 1113 746 2702
Fragments arranged by size:
2702 1113 746 711
BsaBI GATnn'nnATC
Cuts at: 5266 5266
Size: 5272
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2032 2078 3079 3461
Size: 53 83 186 936 499 46 1001 382
Cuts at: 3461 4096 275
Size: 635 1451
Fragments arranged by size:
1451 1001 936 635 499 382 186 83
53 46
BsaWI w'CCGG_w
Cuts at: 3705 4103 4377 4524 4935 3705
Size: 398 274 147 411 4042
Fragments arranged by size:
4042 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5047
Fragments arranged by size:
5047 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5272
BseYI C'CCAG_C
Cuts at: 2483 2511 4475 4971 2483
Size: 28 1964 496 2784
Fragments arranged by size:
2784 1964 496 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2066 1163
Size: 573 330 4369
Fragments arranged by size:
4369 573 330
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2138 2722 3483 4511 1540
Size: 275 186 137 584 761 1028 2301
Fragments arranged by size:
2301 1028 761 584 275 186 137
BsiHKAI G_wGCw'C
Cuts at: 3333 3418 4489 3333
Size: 85 1071 4116
Fragments arranged by size:
4116 1071 85
BsmI GAATG_Cn'
Cuts at: 2783 2876 2783
Size: 93 5179
BsmBI CGTCTCn'nnnn_
Cuts at: 2342 2342
Size: 5272
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2161 2202 2444 2570 2591 3333
Size: 399 251 120 41 242 126 21 742
Cuts at: 3333 3418 4489 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 251 242 126 120
85 41 21
BspEI T'CCGG_A
Cuts at: 4935 4935
Size: 5272
BspHI T'CATG_A
Cuts at: 3161 4891 3161
Size: 1730 3542
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2467 2535 2627 844
Size: 1623 68 92 3489
Fragments arranged by size:
3489 1623 92 68
BsrBI CCG'CTC
Cuts at: 1394 1823 2257 3159 1394
Size: 429 434 902 3507
Fragments arranged by size:
3507 902 434 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3767 3941 65
Size: 1183 2519 174 1396
Fragments arranged by size:
2519 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2019 2113 2133 2137 3916 4103 1700
Size: 110 209 94 20 4 1779 187 2869
Fragments arranged by size:
2869 1779 209 187 110 94 20 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5272
BssHII G'CGCG_C
Cuts at: 1925 2320 1925
Size: 395 4877
BssSI C'ACGA_G
Cuts at: 3332 4344 3332
Size: 1012 4260
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2266 794
Size: 1472 3800
BstBI TT'CG_AA
Cuts at: 4076 4076
Size: 5272
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5272
BstYI r'GATC_y
Cuts at: 1972 2547 2602 2969 3354 3371 4823 4909 4921
Size: 575 55 367 385 17 1452 86 12
Cuts at: 4921 5267 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 575 385 367 346 86 55
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2027 2502 513
Size: 552 962 475 3283
Fragments arranged by size:
3283 962 552 475
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4533
Fragments arranged by size:
4533 683 56
BtsI GCAGTG_nn'
Cuts at: 2788 3582 3602 2788
Size: 794 20 4458
Fragments arranged by size:
4458 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5272
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5237
DraI TTT'AAA
Cuts at: 2576 2576
Size: 5272
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4279 809
Size: 507 2963 1802
Fragments arranged by size:
2963 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 2135 2183
Size: 54 1425 50 275 18 285 20 48
Cuts at: 2183 2206 2361 2388 2719 3608 5237 8
Size: 23 155 27 331 889 1629 43
Fragments arranged by size:
1629 1425 889 331 285 275 155 54
50 48 43 27 23 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 2135 2719 1537
Size: 275 323 584 4090
Fragments arranged by size:
4090 584 323 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3202 1353
Size: 1849 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3859 4233 4379 387
Size: 1064 138 2270 374 146 1280
Fragments arranged by size:
2270 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2258 2450 3335 3932 4719 4987 1239
Size: 393 626 192 885 597 787 268 1524
Fragments arranged by size:
1524 885 787 626 597 393 268 192
EcoO109I rG'GnC_Cy
Cuts at: 2038 2088 2189 2293 2441 2038
Size: 50 101 104 148 4869
Fragments arranged by size:
4869 148 104 101 50
EcoRI G'AATT_C
Cuts at: 2581 2959 2581
Size: 378 4894
FseI GG_CCGG'CC
Cuts at: 2141 2141
Size: 5272
FspI TGC'GCA
Cuts at: 3778 3778
Size: 5272
HaeII r_GCGC'y
Cuts at: 1678 2035 4419 1678
Size: 357 2384 2531
Fragments arranged by size:
2531 2384 357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3913 3945 3987 4019 1373
Size: 32 135 32 2341 32 42 32 2626
Fragments arranged by size:
2626 2341 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2616 2862 669
Size: 988 281 678 246 3079
Fragments arranged by size:
3079 988 678 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5272
HpaI GTT'AAC
Cuts at: 2862 2862
Size: 5272
KasI G'GCGC_C
Cuts at: 2031 2031
Size: 5272
KpnI G_GTAC'C
Cuts at: 2597 2597
Size: 5272
MfeI C'AATT_G
Cuts at: 2871 2871
Size: 5272
MluI A'CGCG_T
Cuts at: 5148 5148
Size: 5272
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4386 4570 1305
Size: 3081 184 2007
Fragments arranged by size:
3081 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 10
Size: 54 1425 343 531 2919
Fragments arranged by size:
2919 1425 531 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3230 3589 3748 518
Size: 648 2064 359 159 2042
Fragments arranged by size:
2064 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2230 2263 2688 3365 4513 4758 1606
Size: 624 33 425 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 624 425 245 33
NaeI GCC'GGC
Cuts at: 2115 2135 2139 2115
Size: 20 4 5248
Fragments arranged by size:
5248 20 4
NarI GG'CG_CC
Cuts at: 2032 2032
Size: 5272
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4720
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5272
NgoMIV G'CCGG_C
Cuts at: 2113 2133 2137 2113
Size: 20 4 5248
Fragments arranged by size:
5248 20 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5272
NotI GC'GGCC_GC
Cuts at: 2719 2719
Size: 5272
NspI r_CATG'y
Cuts at: 1132 1229 2630 4175 1132
Size: 97 1401 1545 2229
Fragments arranged by size:
2229 1545 1401 97
PasI CC'CwG_GG
Cuts at: 1348 2518 1348
Size: 1170 4102
PciI A'CATG_T
Cuts at: 4171 4171
Size: 5272
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5272
PmeI GTTT'AAAC
Cuts at: 2576 2576
Size: 5272
PmlI CAC'GTG
Cuts at: 2317 2317
Size: 5272
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3341 3373 4854 4886 3341
Size: 32 1481 32 3727
Fragments arranged by size:
3727 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2088 2189 2088
Size: 101 5171
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5272
PsiI TTA'TAA
Cuts at: 2842 2842
Size: 5272
PspOMI G'GGCC_C
Cuts at: 2037 2198 2440 2037
Size: 161 242 4869
Fragments arranged by size:
4869 242 161
PstI C_TGCA'G
Cuts at: 830 2532 2624 830
Size: 1702 92 3478
Fragments arranged by size:
3478 1702 92
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5272
SalI G'TCGA_C
Cuts at: 1936 2614 1936
Size: 678 4594
SanDI GG'GwC_CC
Cuts at: 2088 2088
Size: 5272
SbfI CC_TGCA'GG
Cuts at: 2624 2624
Size: 5272
ScaI AGT'ACT
Cuts at: 3520 5232 3520
Size: 1712 3560
SfcI C'TryA_G
Cuts at: 826 1045 2528 2620 3755 4436 4627 826
Size: 219 1483 92 1135 681 191 1471
Fragments arranged by size:
1483 1471 1135 681 219 191 92
SfiI GGCCn_nnn'nGGCC
Cuts at: 2507 2507
Size: 5272
SfoI GGC'GCC
Cuts at: 2033 2033
Size: 5272
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5272
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5272
SmaI CCC'GGG
Cuts at: 1872 2068 2103 2144 2599 1872
Size: 196 35 41 455 4545
Fragments arranged by size:
4545 455 196 41 35
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3376 4277 4539 820
Size: 197 926 1433 901 262 1553
Fragments arranged by size:
1553 1433 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3063 493
Size: 2570 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5272
SphI G_CATG'C
Cuts at: 1132 2630 1132
Size: 1498 3774
SrfI GCCC'GGGC
Cuts at: 2144 2144
Size: 5272
SspI AAT'ATT
Cuts at: 5 52 3196 5
Size: 47 3144 2081
Fragments arranged by size:
3144 2081 47
StuI AGG'CCT
Cuts at: 1517 2427 1517
Size: 910 4362
StyI C'CwwG_G
Cuts at: 513 1065 2118 2711 513
Size: 552 1053 593 3074
Fragments arranged by size:
3074 1053 593 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3299 3316 3469 3654 4099 325
Size: 1255 1719 17 153 185 445 1498
Fragments arranged by size:
1719 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3518 5230 96
Size: 274 80 33 51 1443 1541 1712 138
Fragments arranged by size:
1712 1541 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5232 5264 5232
Size: 32 5240
Tth111I GACn'n_nGTC
Cuts at: 1316 2404 1316
Size: 1088 4184
XbaI T'CTAG_A
Cuts at: 2608 2608
Size: 5272
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5272
XmaI C'CCGG_G
Cuts at: 1870 2066 2101 2142 2597 1870
Size: 196 35 41 455 4545
Fragments arranged by size:
4545 455 196 41 35
XmnI GAAnn'nnTTC
Cuts at: 3401 3401
Size: 5272
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3080 276
Size: 53 83 186 936 1546 2468
Fragments arranged by size:
2468 1546 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FspAI NruI NsiI PacI PfoI PspXI
PsrI PvuII RsrII SacI SacII SapI SexAI SwaI
XcmI