Restriction map for
pFN21AB9782
(Circular) (Six-base) MAPSORT of: pFN21AB9782.seq Check: 9905 from: 1 to: 5332
pFN21AB9782 5332 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3142 278
Size: 53 83 186 936 1606 2468
Fragments arranged by size:
2468 1606 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2675 1937
Size: 738 4594
Acc65I G'GTAC_C
Cuts at: 2653 2653
Size: 5332
AclI AA'CG_TT
Cuts at: 1563 3459 3832 1563
Size: 1896 373 3063
Fragments arranged by size:
3063 1896 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2309 2402 3395 4779 5047 1632
Size: 677 93 993 1384 268 1917
Fragments arranged by size:
1917 1384 993 677 268 93
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5135
AflIII A'CryG_T
Cuts at: 1203 4231 5208 1203
Size: 3028 977 1327
Fragments arranged by size:
3028 1327 977
AgeI A'CCGG_T
Cuts at: 4163 4163
Size: 5332
AhdI GACnn_n'nnGTC
Cuts at: 4061 4061
Size: 5332
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5332
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5332
AlwNI CAG_nnn'CTG
Cuts at: 3075 4647 3075
Size: 1572 3760
ApaI G_GGCC'C
Cuts at: 2255 2598 2255
Size: 343 4989
ApaLI G'TGCA_C
Cuts at: 2303 3389 4545 2303
Size: 1086 1156 3090
Fragments arranged by size:
3090 1156 1086
ApoI r'AATT_y
Cuts at: 1493 2641 2867 3019 5128 1493
Size: 1148 226 152 2109 1697
Fragments arranged by size:
2109 1697 1148 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3886 160
Size: 872 2854 1606
Fragments arranged by size:
2854 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5332
AvaI C'yCGr_G
Cuts at: 1870 1943 2657 1870
Size: 73 714 4545
Fragments arranged by size:
4545 714 73
BamHI G'GATC_C
Cuts at: 2662 3029 2662
Size: 367 4965
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2242 2388 2570 2653 4108
Size: 325 217 627 455 146 182 83 1455
Cuts at: 4108 618
Size: 1842
Fragments arranged by size:
1842 1455 627 455 325 217 182 146
83
BanII G_rGCy'C
Cuts at: 1391 2255 2447 2598 2651 1391
Size: 864 192 151 53 4072
Fragments arranged by size:
4072 864 192 151 53
BbeI G_GCGC'C
Cuts at: 2392 2392
Size: 5332
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2163 928
Size: 971 264 4097
Fragments arranged by size:
4097 971 264
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2354 2388 3523 3557 1903
Size: 34 417 34 1135 34 3678
Fragments arranged by size:
3678 1135 417 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3224 4434 3224
Size: 1210 4122
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5332
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3943 136
Size: 107 122 71 631 605 2271 1525
Fragments arranged by size:
2271 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5327 5327
Size: 5332
BlpI GC'TnA_GC
Cuts at: 2279 2749 2279
Size: 470 4862
Bme1580I G_kGCm'C
Cuts at: 1790 2247 2255 2307 2341 2598 3393 4549 1790
Size: 457 8 52 34 257 795 1156 2573
Fragments arranged by size:
2573 1156 795 457 257 52 34 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5332
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3063 4021 442
Size: 416 53 83 399 24 1646 958 1753
Fragments arranged by size:
1753 1646 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5332
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 2225 2032
Size: 193 5139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2072 2339 2414 3992 1239
Size: 815 18 267 75 1578 2579
Fragments arranged by size:
2579 1578 815 267 75 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2216 2243 3457 4322 4620 2216
Size: 27 1214 865 298 2928
Fragments arranged by size:
2928 1214 865 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3995 882
Size: 617 2496 2219
Fragments arranged by size:
2496 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3123 493
Size: 711 1919 2702
Fragments arranged by size:
2702 1919 711
BsaBI GATnn'nnATC
Cuts at: 5326 5326
Size: 5332
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2389 3139 3521 4156
Size: 53 83 186 936 856 750 382 635
Cuts at: 4156 275
Size: 1451
Fragments arranged by size:
1451 936 856 750 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3765 4163 4437 4584 4995 3765
Size: 398 274 147 411 4102
Fragments arranged by size:
4102 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2387 2417 1178
Size: 30 165 30 984 30 4093
Fragments arranged by size:
4093 984 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2336 1186
Size: 1150 4182
BseYI C'CCAG_C
Cuts at: 2611 4535 5031 2611
Size: 1924 496 2912
Fragments arranged by size:
2912 1924 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2429 1163
Size: 573 693 4066
Fragments arranged by size:
4066 693 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2043 2139 2782 3543 4571 1540
Size: 275 186 42 96 643 761 1028 2301
Fragments arranged by size:
2301 1028 761 643 275 186 96 42
BsiHKAI G_wGCw'C
Cuts at: 2079 2307 3393 3478 4549 2079
Size: 228 1086 85 1071 2862
Fragments arranged by size:
2862 1086 1071 228 85
BsmI GAATG_Cn'
Cuts at: 2843 2936 2843
Size: 93 5239
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2247 2255 2307 2341 2447 2598
Size: 399 289 168 8 52 34 106 151
Cuts at: 2598 2651 3393 3478 4549 1391
Size: 53 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 289 168 151 106
85 53 52 34 8
BspEI T'CCGG_A
Cuts at: 4995 4995
Size: 5332
BspHI T'CATG_A
Cuts at: 3221 4951 3221
Size: 1730 3602
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2687 844
Size: 1843 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3219 1394
Size: 429 1396 3507
Fragments arranged by size:
3507 1396 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3827 4001 65
Size: 1183 2579 174 1396
Fragments arranged by size:
2579 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2138 2573 2604 3976 4163 1700
Size: 110 328 435 31 1372 187 2869
Fragments arranged by size:
2869 1372 435 328 187 110 31
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5332
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5332
BssSI C'ACGA_G
Cuts at: 3392 4404 3392
Size: 1012 4320
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2242 794
Size: 1448 3884
BstBI TT'CG_AA
Cuts at: 4136 4136
Size: 5332
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5332
BstYI r'GATC_y
Cuts at: 1972 2662 3029 3414 3431 4883 4969 4981 5327
Size: 690 367 385 17 1452 86 12 346
Cuts at: 5327 1972
Size: 1977
Fragments arranged by size:
1977 1452 690 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2457 513
Size: 552 939 453 3388
Fragments arranged by size:
3388 939 552 453
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4593
Fragments arranged by size:
4593 683 56
BtsI GCAGTG_nn'
Cuts at: 2542 2599 2848 3642 3662 2542
Size: 57 249 794 20 4212
Fragments arranged by size:
4212 794 249 57 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5332
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5297
DraI TTT'AAA
Cuts at: 2636 2636
Size: 5332
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4339 809
Size: 507 3023 1802
Fragments arranged by size:
3023 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2136 2779
Size: 54 1425 50 275 18 210 96 643
Cuts at: 2779 3668 5297 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 643 275 210 96 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2040 2136 2779 1537
Size: 275 228 96 643 4090
Fragments arranged by size:
4090 643 275 228 96
EarI CTCTTCn'nnn_
Cuts at: 1353 3262 1353
Size: 1909 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3919 4293 4439 387
Size: 1064 138 2330 374 146 1280
Fragments arranged by size:
2330 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2072 2309 2339 2402 2414 3395
Size: 393 422 18 237 30 63 12 981
Cuts at: 3395 3992 4779 5047 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 981 787 597 422 393 268 237
63 30 18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2033 2033
Size: 5332
EcoO109I rG'GnC_Cy
Cuts at: 2155 2252 2595 2155
Size: 97 343 4892
Fragments arranged by size:
4892 343 97
EcoRI G'AATT_C
Cuts at: 2641 3019 2641
Size: 378 4954
FseI GG_CCGG'CC
Cuts at: 2142 2142
Size: 5332
FspI TGC'GCA
Cuts at: 2150 2429 3838 2150
Size: 279 1409 3644
Fragments arranged by size:
3644 1409 279
FspAI rTGC'GCAy
Cuts at: 2429 2429
Size: 5332
HaeII r_GCGC'y
Cuts at: 1678 2392 4479 1678
Size: 714 2087 2531
Fragments arranged by size:
2531 2087 714
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2387 2419 3973 4005 4047
Size: 32 135 32 815 32 1554 32 42
Cuts at: 4047 4079 1373
Size: 32 2626
Fragments arranged by size:
2626 1554 815 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2676 2922 669
Size: 988 281 738 246 3079
Fragments arranged by size:
3079 988 738 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5332
HpaI GTT'AAC
Cuts at: 2922 2922
Size: 5332
KasI G'GCGC_C
Cuts at: 2388 2388
Size: 5332
KpnI G_GTAC'C
Cuts at: 2657 2657
Size: 5332
MfeI C'AATT_G
Cuts at: 2931 2931
Size: 5332
MluI A'CGCG_T
Cuts at: 5208 5208
Size: 5332
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4446 4630 1305
Size: 3141 184 2007
Fragments arranged by size:
3141 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3510
Fragments arranged by size:
3510 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3290 3649 3808 518
Size: 648 2124 359 159 2042
Fragments arranged by size:
2124 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2359 2401 2748 3425 4573 4818 1606
Size: 753 42 347 677 1148 245 2120
Fragments arranged by size:
2120 1148 753 677 347 245 42
NaeI GCC'GGC
Cuts at: 2140 2606 2140
Size: 466 4866
NarI GG'CG_CC
Cuts at: 2389 2389
Size: 5332
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3841
Fragments arranged by size:
3841 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5332
NgoMIV G'CCGG_C
Cuts at: 2138 2604 2138
Size: 466 4866
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5332
NotI GC'GGCC_GC
Cuts at: 2779 2779
Size: 5332
NspI r_CATG'y
Cuts at: 1132 1229 2690 4235 1132
Size: 97 1461 1545 2229
Fragments arranged by size:
2229 1545 1461 97
PasI CC'CwG_GG
Cuts at: 1348 2248 1348
Size: 900 4432
PciI A'CATG_T
Cuts at: 4231 4231
Size: 5332
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5332
PmeI GTTT'AAAC
Cuts at: 2636 2636
Size: 5332
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3401 3433 4914 4946 3401
Size: 32 1481 32 3787
Fragments arranged by size:
3787 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5332
PsiI TTA'TAA
Cuts at: 2902 2902
Size: 5332
PspOMI G'GGCC_C
Cuts at: 2251 2594 2251
Size: 343 4989
PstI C_TGCA'G
Cuts at: 830 2364 2565 2684 830
Size: 1534 201 119 3478
Fragments arranged by size:
3478 1534 201 119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5332
PvuII CAG'CTG
Cuts at: 2359 2401 2359
Size: 42 5290
SalI G'TCGA_C
Cuts at: 1936 2674 1936
Size: 738 4594
SbfI CC_TGCA'GG
Cuts at: 2684 2684
Size: 5332
ScaI AGT'ACT
Cuts at: 3580 5292 3580
Size: 1712 3620
SfcI C'TryA_G
Cuts at: 826 1045 2360 2554 2561 2680 3815 4496 4687
Size: 219 1315 194 7 119 1135 681 191
Cuts at: 4687 826
Size: 1471
Fragments arranged by size:
1471 1315 1135 681 219 194 191 119
7
SfoI GGC'GCC
Cuts at: 2390 2390
Size: 5332
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5332
SgrAI Cr'CCGG_yG
Cuts at: 1700 2573 1700
Size: 873 4459
SmaI CCC'GGG
Cuts at: 1872 2659 1872
Size: 787 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2222 2231 3436 4337 4599 820
Size: 197 926 279 9 1205 901 262 1553
Fragments arranged by size:
1553 1205 926 901 279 262 197 9
SnaBI TAC'GTA
Cuts at: 493 3123 493
Size: 2630 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5332
SphI G_CATG'C
Cuts at: 1132 2690 1132
Size: 1558 3774
SspI AAT'ATT
Cuts at: 5 52 3256 5
Size: 47 3204 2081
Fragments arranged by size:
3204 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5332
StyI C'CwwG_G
Cuts at: 513 1065 2004 2298 2771 513
Size: 552 939 294 473 3074
Fragments arranged by size:
3074 939 552 473 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3359 3376 3529 3714 4159 325
Size: 1255 1779 17 153 185 445 1498
Fragments arranged by size:
1779 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3578 5290 96
Size: 274 80 33 51 1443 1601 1712 138
Fragments arranged by size:
1712 1601 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5292 5324 5292
Size: 32 5300
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5332
XbaI T'CTAG_A
Cuts at: 2668 2668
Size: 5332
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5332
XmaI C'CCGG_G
Cuts at: 1870 2657 1870
Size: 787 4545
XmnI GAAnn'nnTTC
Cuts at: 3461 3461
Size: 5332
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3140 276
Size: 53 83 186 936 1606 2468
Fragments arranged by size:
2468 1606 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI