Restriction map for
pFN21AB9778
(Circular) (Six-base) MAPSORT of: pFN21AB9778.seq Check: 4587 from: 1 to: 7099
pFN21AB9778 7099 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3462 3462
Size: 7099
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4909 278
Size: 53 83 186 936 3373 2468
Fragments arranged by size:
3373 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2119 3496 4442 1937
Size: 182 1377 946 4594
Fragments arranged by size:
4594 1377 946 182
Acc65I G'GTAC_C
Cuts at: 4420 4420
Size: 7099
AclI AA'CG_TT
Cuts at: 1563 5226 5599 1563
Size: 3663 373 3063
Fragments arranged by size:
3663 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2439 2527 3039 3362 5162 6546 6814 1632
Size: 807 88 512 323 1800 1384 268 1917
Fragments arranged by size:
1917 1800 1384 807 512 323 268 88
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6902
AflIII A'CryG_T
Cuts at: 1203 3890 5998 6975 1203
Size: 2687 2108 977 1327
Fragments arranged by size:
2687 2108 1327 977
AgeI A'CCGG_T
Cuts at: 5930 5930
Size: 7099
AhdI GACnn_n'nnGTC
Cuts at: 5828 5828
Size: 7099
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7099
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7099
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3258 3290 3258
Size: 32 7067
AlwNI CAG_nnn'CTG
Cuts at: 2980 3017 4842 6414 2980
Size: 37 1825 1572 3665
Fragments arranged by size:
3665 1825 1572 37
ApaLI G'TGCA_C
Cuts at: 2146 5156 6312 2146
Size: 3010 1156 2933
Fragments arranged by size:
3010 2933 1156
ApoI r'AATT_y
Cuts at: 1493 2008 2152 2405 2734 3023 3642 3656 4298
Size: 515 144 253 329 289 619 14 642
Cuts at: 4298 4408 4634 4786 6895 1493
Size: 110 226 152 2109 1697
Fragments arranged by size:
2109 1697 642 619 515 329 289 253
226 152 144 110 14
AseI AT'TA_AT
Cuts at: 160 1032 5653 160
Size: 872 4621 1606
Fragments arranged by size:
4621 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7099
AvaI C'yCGr_G
Cuts at: 1870 1943 4424 1870
Size: 73 2481 4545
Fragments arranged by size:
4545 2481 73
BamHI G'GATC_C
Cuts at: 4429 4796 4429
Size: 367 6732
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4420 5875 618
Size: 325 217 627 2633 1455 1842
Fragments arranged by size:
2633 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4418 1391
Size: 3027 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2427 3540 928
Size: 971 528 1113 4487
Fragments arranged by size:
4487 1113 971 528
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5290 5324 1903
Size: 34 3353 34 3678
Fragments arranged by size:
3678 3353 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3781 4991 6201 3781
Size: 1210 1210 4679
Fragments arranged by size:
4679 1210 1210
BclI T'GATC_A
Cuts at: 1548 2037 3114 1548
Size: 489 1077 5533
Fragments arranged by size:
5533 1077 489
BfrBI ATG'CAT
Cuts at: 3125 3125
Size: 7099
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5710 136
Size: 107 122 71 631 605 4038 1525
Fragments arranged by size:
4038 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2506 3819 7094 2506
Size: 1313 3275 2511
Fragments arranged by size:
3275 2511 1313
BlpI GC'TnA_GC
Cuts at: 2607 3927 4516 2607
Size: 1320 589 5190
Fragments arranged by size:
5190 1320 589
Bme1580I G_kGCm'C
Cuts at: 1790 2150 5160 6316 1790
Size: 360 3010 1156 2573
Fragments arranged by size:
3010 2573 1156 360
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7099
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4830 5788 442
Size: 416 53 83 399 24 3413 958 1753
Fragments arranged by size:
3413 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7099
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2217 2730 2824 2880 5759 1239
Size: 978 513 94 56 2879 2579
Fragments arranged by size:
2879 2579 978 513 94 56
Bpu10I CC'TnA_GC
Cuts at: 2612 2612
Size: 7099
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2306 5224 6089 6387 2306
Size: 2918 865 298 3018
Fragments arranged by size:
3018 2918 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5762 882
Size: 617 4263 2219
Fragments arranged by size:
4263 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4116 4890 493
Size: 711 2912 774 2702
Fragments arranged by size:
2912 2702 774 711
BsaBI GATnn'nnATC
Cuts at: 2374 7093 2374
Size: 4719 2380
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4906 5288 5923 275
Size: 53 83 186 936 3373 382 635 1451
Fragments arranged by size:
3373 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5532 5930 6204 6351 6762 5532
Size: 398 274 147 411 5869
Fragments arranged by size:
5869 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3258 3288 3582 3612 1178
Size: 30 165 30 1855 30 294 30 4665
Fragments arranged by size:
4665 1855 294 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2539 1186
Size: 1353 5746
BseYI C'CCAG_C
Cuts at: 4044 6302 6798 4044
Size: 2258 496 4345
Fragments arranged by size:
4345 2258 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2172 2626 3490 3970 1163
Size: 573 436 454 864 480 4292
Fragments arranged by size:
4292 864 573 480 454 436
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4549 5310 6338 1540
Size: 275 186 2548 761 1028 2301
Fragments arranged by size:
2548 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2150 2673 5160 5245 6316 2150
Size: 523 2487 85 1071 2933
Fragments arranged by size:
2933 2487 1071 523 85
BsmI GAATG_Cn'
Cuts at: 2181 3404 3512 4324 4610 4703 2181
Size: 1223 108 812 286 93 4577
Fragments arranged by size:
4577 1223 812 286 108 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2150 2673 4418 5160 5245 6316 1391
Size: 399 360 523 1745 742 85 1071 2174
Fragments arranged by size:
2174 1745 1071 742 523 399 360 85
BspEI T'CCGG_A
Cuts at: 6762 6762
Size: 7099
BspHI T'CATG_A
Cuts at: 3436 4276 4988 6718 3436
Size: 840 712 1730 3817
Fragments arranged by size:
3817 1730 840 712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3462 3917 4454 844
Size: 2618 455 537 3489
Fragments arranged by size:
3489 2618 537 455
BsrBI CCG'CTC
Cuts at: 1394 1823 4986 1394
Size: 429 3163 3507
Fragments arranged by size:
3507 3163 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3106 3484 3832 5594 5768 65
Size: 1183 1858 378 348 1762 174 1396
Fragments arranged by size:
1858 1762 1396 1183 378 348 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4048 5743 5930 1700
Size: 110 2238 1695 187 2869
Fragments arranged by size:
2869 2238 1695 187 110
BsrGI T'GTAC_A
Cuts at: 96 2925 3863 96
Size: 2829 938 3332
Fragments arranged by size:
3332 2829 938
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7099
BssSI C'ACGA_G
Cuts at: 5159 6171 5159
Size: 1012 6087
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3660 794
Size: 2866 4233
BstBI TT'CG_AA
Cuts at: 5903 5903
Size: 7099
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7099
BstXI CCAn_nnnn'nTGG
Cuts at: 2025 2025
Size: 7099
BstYI r'GATC_y
Cuts at: 1972 2343 2506 3689 3819 3868 4429 4796 5181
Size: 371 163 1183 130 49 561 367 385
Cuts at: 5181 5198 6650 6736 6748 7094 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1183 561 385 371 367 346
163 130 86 49 17 12
BstZ17I GTA'TAC
Cuts at: 2120 2120
Size: 7099
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6547
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6360
Fragments arranged by size:
6360 683 56
BtsI GCAGTG_nn'
Cuts at: 3772 4615 5409 5429 3772
Size: 843 794 20 5442
Fragments arranged by size:
5442 843 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7099
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2089 2124 2340 2375 529
Size: 35 1525 35 216 35 5253
Fragments arranged by size:
5253 1525 216 35 35 35
DraI TTT'AAA
Cuts at: 2088 3334 4403 2088
Size: 1246 1069 4784
Fragments arranged by size:
4784 1246 1069
DraIII CAC_nnn'GTG
Cuts at: 2195 2195
Size: 7099
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6106 809
Size: 507 4790 1802
Fragments arranged by size:
4790 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3829 4546 5435
Size: 54 1425 50 275 18 1999 717 889
Cuts at: 5435 7064 8
Size: 1629 43
Fragments arranged by size:
1999 1629 1425 889 717 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4546 1537
Size: 275 2734 4090
Fragments arranged by size:
4090 2734 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3617 5029 1353
Size: 2264 1412 3423
Fragments arranged by size:
3423 2264 1412
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5686 6060 6206 387
Size: 1064 138 4097 374 146 1280
Fragments arranged by size:
4097 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2217 2439 2527 2730 2824 2880 3039
Size: 393 585 222 88 203 94 56 159
Cuts at: 3039 3362 5162 5759 6546 6814 1239
Size: 323 1800 597 787 268 1524
Fragments arranged by size:
1800 1524 787 597 585 393 323 268
222 203 159 94 88 56
EcoRI G'AATT_C
Cuts at: 4408 4786 4408
Size: 378 6721
FspI TGC'GCA
Cuts at: 5605 5605
Size: 7099
HaeII r_GCGC'y
Cuts at: 1678 6246 1678
Size: 4568 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3582 3614 4052 4084 5740
Size: 32 135 32 2010 32 438 32 1656
Cuts at: 5740 5772 5814 5846 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2010 1656 438 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3526 4093 4443 4689 669
Size: 988 281 1588 567 350 246 3079
Fragments arranged by size:
3079 1588 988 567 350 281 246
HindIII A'AGCT_T
Cuts at: 748 4259 748
Size: 3511 3588
HpaI GTT'AAC
Cuts at: 3526 4689 3526
Size: 1163 5936
KpnI G_GTAC'C
Cuts at: 4424 4424
Size: 7099
MfeI C'AATT_G
Cuts at: 4197 4698 4197
Size: 501 6598
MluI A'CGCG_T
Cuts at: 6975 6975
Size: 7099
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2371 2692 4078 6213 6397 1305
Size: 1066 321 1386 2135 184 2007
Fragments arranged by size:
2135 2007 1386 1066 321 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5277
Fragments arranged by size:
5277 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2943 3457 3994 5057 5416 5575 518
Size: 648 1777 514 537 1063 359 159 2042
Fragments arranged by size:
2042 1777 1063 648 537 514 359 159
MspA1I CmG'CkG
Cuts at: 1606 4515 5192 6340 6585 1606
Size: 2909 677 1148 245 2120
Fragments arranged by size:
2909 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6547
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7099
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7099
NotI GC'GGCC_GC
Cuts at: 4546 4546
Size: 7099
NsiI A_TGCA'T
Cuts at: 3127 3127
Size: 7099
NspI r_CATG'y
Cuts at: 1132 1229 3774 3987 4457 6002 1132
Size: 97 2545 213 470 1545 2229
Fragments arranged by size:
2545 2229 1545 470 213 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7099
PciI A'CATG_T
Cuts at: 5998 5998
Size: 7099
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2138 2753 2897 1108
Size: 1030 615 144 5310
Fragments arranged by size:
5310 1030 615 144
PmeI GTTT'AAAC
Cuts at: 4403 4403
Size: 7099
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3258 3290 5168 5200 6681 6713 3258
Size: 32 1878 32 1481 32 3644
Fragments arranged by size:
3644 1878 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7099
PsiI TTA'TAA
Cuts at: 4353 4669 4353
Size: 316 6783
PstI C_TGCA'G
Cuts at: 830 3554 3581 4451 830
Size: 2724 27 870 3478
Fragments arranged by size:
3478 2724 870 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7099
SalI G'TCGA_C
Cuts at: 1936 4441 1936
Size: 2505 4594
SbfI CC_TGCA'GG
Cuts at: 4451 4451
Size: 7099
ScaI AGT'ACT
Cuts at: 3796 5347 7059 3796
Size: 1551 1712 3836
Fragments arranged by size:
3836 1712 1551
SfcI C'TryA_G
Cuts at: 826 1045 3550 3577 4447 5582 6263 6454 826
Size: 219 2505 27 870 1135 681 191 1471
Fragments arranged by size:
2505 1471 1135 870 681 219 191 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7099
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7099
SmaI CCC'GGG
Cuts at: 1872 4426 1872
Size: 2554 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2321 5203 6104 6366 820
Size: 197 926 378 2882 901 262 1553
Fragments arranged by size:
2882 1553 926 901 378 262 197
SnaBI TAC'GTA
Cuts at: 493 4116 4890 493
Size: 3623 774 2702
Fragments arranged by size:
3623 2702 774
SpeI A'CTAG_T
Cuts at: 152 3791 152
Size: 3639 3460
SphI G_CATG'C
Cuts at: 1132 4457 1132
Size: 3325 3774
SspI AAT'ATT
Cuts at: 5 52 2995 3256 5023 5
Size: 47 2943 261 1767 2081
Fragments arranged by size:
2943 2081 1767 261 47
StuI AGG'CCT
Cuts at: 1517 3343 1517
Size: 1826 5273
StyI C'CwwG_G
Cuts at: 513 1065 3913 4017 4538 513
Size: 552 2848 104 521 3074
Fragments arranged by size:
3074 2848 552 521 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2130 5126 5143 5296 5481 5926 325
Size: 1255 550 2996 17 153 185 445 1498
Fragments arranged by size:
2996 1498 1255 550 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2925 3381 3794
Size: 274 80 33 51 1443 948 456 413
Cuts at: 3794 3863 5345 7057 96
Size: 69 1482 1712 138
Fragments arranged by size:
1712 1482 1443 948 456 413 274 138
80 69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2860 2892 7059 7091 2860
Size: 32 4167 32 2868
Fragments arranged by size:
4167 2868 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2904 1316
Size: 1588 5511
XbaI T'CTAG_A
Cuts at: 4435 4435
Size: 7099
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2139 2139
Size: 7099
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7099
XmaI C'CCGG_G
Cuts at: 1870 4424 1870
Size: 2554 4545
XmnI GAAnn'nnTTC
Cuts at: 3024 3247 3942 5228 3024
Size: 223 695 1286 4895
Fragments arranged by size:
4895 1286 695 223
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4907 276
Size: 53 83 186 936 3373 2468
Fragments arranged by size:
3373 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BsmBI Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI