Restriction map for
pFN21AB9777
(Circular) (Six-base) MAPSORT of: pFN21AB9777.seq Check: 4147 from: 1 to: 7069
pFN21AB9777 7069 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4879 278
Size: 53 83 186 936 3343 2468
Fragments arranged by size:
3343 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4092 4412 1937
Size: 2155 320 4594
Fragments arranged by size:
4594 2155 320
Acc65I G'GTAC_C
Cuts at: 4390 4390
Size: 7069
AclI AA'CG_TT
Cuts at: 1563 5196 5569 1563
Size: 3633 373 3063
Fragments arranged by size:
3633 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2012 2481 2876 2933 3506 5132 6516 6784
Size: 380 469 395 57 573 1626 1384 268
Cuts at: 6784 1632
Size: 1917
Fragments arranged by size:
1917 1626 1384 573 469 395 380 268
57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6872
AflIII A'CryG_T
Cuts at: 1203 5968 6945 1203
Size: 4765 977 1327
Fragments arranged by size:
4765 1327 977
AgeI A'CCGG_T
Cuts at: 5900 5900
Size: 7069
AhdI GACnn_n'nnGTC
Cuts at: 5798 5798
Size: 7069
AleI CACnn'nnGTG
Cuts at: 1166 2574 3283 1166
Size: 1408 709 4952
Fragments arranged by size:
4952 1408 709
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7069
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3728 3760 3728
Size: 32 7037
AlwNI CAG_nnn'CTG
Cuts at: 2842 3331 4812 6384 2842
Size: 489 1481 1572 3527
Fragments arranged by size:
3527 1572 1481 489
ApaI G_GGCC'C
Cuts at: 3306 3306
Size: 7069
ApaLI G'TGCA_C
Cuts at: 5126 6282 5126
Size: 1156 5913
ApoI r'AATT_y
Cuts at: 1493 2263 2311 3128 4378 4604 4756 6865 1493
Size: 770 48 817 1250 226 152 2109 1697
Fragments arranged by size:
2109 1697 1250 817 770 226 152 48
AseI AT'TA_AT
Cuts at: 160 1032 2271 5623 160
Size: 872 1239 3352 1606
Fragments arranged by size:
3352 1606 1239 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7069
AvaI C'yCGr_G
Cuts at: 1870 1943 2099 2810 3309 3978 4394 1870
Size: 73 156 711 499 669 416 4545
Fragments arranged by size:
4545 711 669 499 416 156 73
BamHI G'GATC_C
Cuts at: 4007 4399 4766 4007
Size: 392 367 6310
Fragments arranged by size:
6310 392 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3034 4390 5845 618
Size: 325 217 627 1247 1356 1455 1842
Fragments arranged by size:
1842 1455 1356 1247 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2307 2934 3306 4388 1391
Size: 916 627 372 1082 4072
Fragments arranged by size:
4072 1082 916 627 372
BbeI G_GCGC'C
Cuts at: 3038 3038
Size: 7069
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2313 2882 3281 3300 3494 928
Size: 971 414 569 399 19 194 4503
Fragments arranged by size:
4503 971 569 414 399 194 19
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5260 5294 1903
Size: 34 3323 34 3678
Fragments arranged by size:
3678 3323 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4961 6171 4961
Size: 1210 5859
BclI T'GATC_A
Cuts at: 1548 2833 1548
Size: 1285 5784
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5680 136
Size: 107 122 71 631 605 4008 1525
Fragments arranged by size:
4008 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7064 7064
Size: 7069
BlpI GC'TnA_GC
Cuts at: 2996 4486 2996
Size: 1490 5579
Bme1580I G_kGCm'C
Cuts at: 1790 3306 5130 6286 1790
Size: 1516 1824 1156 2573
Fragments arranged by size:
2573 1824 1516 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7069
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2746 3319 3534
Size: 416 53 83 399 24 1329 573 215
Cuts at: 3534 4800 5758 442
Size: 1266 958 1753
Fragments arranged by size:
1753 1329 1266 958 573 416 399 215
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7069
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2232 2714 2882 3353 3567 3746 4384 5729
Size: 993 482 168 471 214 179 638 1345
Cuts at: 5729 1239
Size: 2579
Fragments arranged by size:
2579 1345 993 638 482 471 214 179
168
Bpu10I CC'TnA_GC
Cuts at: 2485 2485
Size: 7069
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2291 5194 6059 6357 2291
Size: 2903 865 298 3003
Fragments arranged by size:
3003 2903 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2954 4029 5732 882
Size: 617 1455 1075 1703 2219
Fragments arranged by size:
2219 1703 1455 1075 617
BsaAI yAC'GTr
Cuts at: 493 1204 4860 493
Size: 711 3656 2702
Fragments arranged by size:
3656 2702 711
BsaBI GATnn'nnATC
Cuts at: 7063 7063
Size: 7069
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3035 4876 5258 5893
Size: 53 83 186 936 1502 1841 382 635
Cuts at: 5893 275
Size: 1451
Fragments arranged by size:
1841 1502 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2742 4325 5502 5900 6174 6321 6732 2742
Size: 1583 1177 398 274 147 411 3079
Fragments arranged by size:
3079 1583 1177 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2205 2235 3560 3590 3728
Size: 30 165 30 802 30 1325 30 138
Cuts at: 3728 3758 1178
Size: 30 4489
Fragments arranged by size:
4489 1325 802 165 138 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2435 3012 3833 4259 1186
Size: 1249 577 821 426 3996
Fragments arranged by size:
3996 1249 821 577 426
BseYI C'CCAG_C
Cuts at: 2944 3038 3739 6272 6768 2944
Size: 94 701 2533 496 3245
Fragments arranged by size:
3245 2533 701 496 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2804 1163
Size: 573 1068 5428
Fragments arranged by size:
5428 1068 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4519 5280 6308 1540
Size: 275 186 2518 761 1028 2301
Fragments arranged by size:
2518 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2307 2934 3290 3581 3627 5130 5215 6286 2307
Size: 627 356 291 46 1503 85 1071 3090
Fragments arranged by size:
3090 1503 1071 627 356 291 85 46
BsmI GAATG_Cn'
Cuts at: 2955 3612 4580 4673 2955
Size: 657 968 93 5351
Fragments arranged by size:
5351 968 657 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2307 2934 3290 3306 3581 3627 4388
Size: 399 517 627 356 16 275 46 761
Cuts at: 4388 5130 5215 6286 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 761 742 627 517 399 356
275 85 46 16
BspEI T'CCGG_A
Cuts at: 4325 6732 4325
Size: 2407 4662
BspHI T'CATG_A
Cuts at: 4958 6688 4958
Size: 1730 5339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2644 3337 4424 844
Size: 1800 693 1087 3489
Fragments arranged by size:
3489 1800 1087 693
BsrBI CCG'CTC
Cuts at: 1394 1823 4956 1394
Size: 429 3133 3507
Fragments arranged by size:
3507 3133 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2305 3841 5564 5738 65
Size: 1183 1057 1536 1723 174 1396
Fragments arranged by size:
1723 1536 1396 1183 1057 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2632 2778 5713 5900 1700
Size: 110 822 146 2935 187 2869
Fragments arranged by size:
2935 2869 822 187 146 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7069
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7069
BssSI C'ACGA_G
Cuts at: 3620 5129 6141 3620
Size: 1509 1012 4548
Fragments arranged by size:
4548 1509 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7069
BstBI TT'CG_AA
Cuts at: 5873 5873
Size: 7069
BstEII G'GTnAC_C
Cuts at: 1184 2579 1184
Size: 1395 5674
BstXI CCAn_nnnn'nTGG
Cuts at: 2860 2860
Size: 7069
BstYI r'GATC_y
Cuts at: 1972 3193 3481 4007 4322 4399 4766 5151 5168
Size: 1221 288 526 315 77 367 385 17
Cuts at: 5168 6620 6706 6718 7064 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1221 526 385 367 346 315
288 86 77 17 12
Bsu36I CC'TnA_GG
Cuts at: 2112 2409 3435 2112
Size: 297 1026 5746
Fragments arranged by size:
5746 1026 297
BtgI C'CryG_G
Cuts at: 513 1065 3030 513
Size: 552 1965 4552
Fragments arranged by size:
4552 1965 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6330
Fragments arranged by size:
6330 683 56
BtsI GCAGTG_nn'
Cuts at: 2581 2614 3912 4585 5379 5399 2581
Size: 33 1298 673 794 20 4251
Fragments arranged by size:
4251 1298 794 673 33 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7069
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7034
DraI TTT'AAA
Cuts at: 4165 4373 4165
Size: 208 6861
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2743 6076 809
Size: 507 1427 3333 1802
Fragments arranged by size:
3333 1802 1427 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3694 4069 4516
Size: 54 1425 50 275 18 1864 375 447
Cuts at: 4516 5405 7034 8
Size: 889 1629 43
Fragments arranged by size:
1864 1629 1425 889 447 375 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4516 1537
Size: 275 2704 4090
Fragments arranged by size:
4090 2704 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2030 2376 2667 2909 3444 4236 4999 1353
Size: 677 346 291 242 535 792 763 3423
Fragments arranged by size:
3423 792 763 677 535 346 291 242
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3874 5656 6030 6176 387
Size: 1064 138 2285 1782 374 146 1280
Fragments arranged by size:
2285 1782 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2305 2932 2305
Size: 627 6442
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2012 2232 2481 2714 2876 2882 2933
Size: 393 380 220 249 233 162 6 51
Cuts at: 2933 3353 3506 3567 3746 4384 5132 5729 6516
Size: 420 153 61 179 638 748 597 787
Cuts at: 6516 6784 1239
Size: 268 1524
Fragments arranged by size:
1524 787 748 638 597 420 393 380
268 249 233 220 179 162 153 61
51 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2337 2337
Size: 7069
EcoO109I rG'GnC_Cy
Cuts at: 2085 2413 2794 3302 2085
Size: 328 381 508 5852
Fragments arranged by size:
5852 508 381 328
EcoRI G'AATT_C
Cuts at: 2311 4378 4756 2311
Size: 2067 378 4624
Fragments arranged by size:
4624 2067 378
EcoRV GAT'ATC
Cuts at: 3871 3871
Size: 7069
FspI TGC'GCA
Cuts at: 5575 5575
Size: 7069
HaeII r_GCGC'y
Cuts at: 1678 2533 3038 3725 6216 1678
Size: 855 505 687 2491 2531
Fragments arranged by size:
2531 2491 855 687 505
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3565 3597 4256 4288 4307
Size: 32 135 32 1993 32 659 32 19
Cuts at: 4307 4339 5710 5742 5784 5816 1373
Size: 32 1371 32 42 32 2626
Fragments arranged by size:
2626 1993 1371 659 135 42 32 32
32 32 32 32 32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 4093 4413 4659 669
Size: 988 281 2155 320 246 3079
Fragments arranged by size:
3079 2155 988 320 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7069
HpaI GTT'AAC
Cuts at: 4659 4659
Size: 7069
KasI G'GCGC_C
Cuts at: 3034 3034
Size: 7069
KpnI G_GTAC'C
Cuts at: 4394 4394
Size: 7069
MfeI C'AATT_G
Cuts at: 4668 4668
Size: 7069
MluI A'CGCG_T
Cuts at: 6945 6945
Size: 7069
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2117 2336 3459 6183 6367 1305
Size: 812 219 1123 2724 184 2007
Fragments arranged by size:
2724 2007 1123 812 219 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3696 4071 10
Size: 54 1425 343 1864 375 3008
Fragments arranged by size:
3008 1864 1425 375 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2160 2574 3283 5027 5386 5545 518
Size: 648 994 414 709 1744 359 159 2042
Fragments arranged by size:
2042 1744 994 709 648 414 359 159
MspA1I CmG'CkG
Cuts at: 1606 2860 2944 3076 4024 4485 5162 6310 6555
Size: 1254 84 132 948 461 677 1148 245
Cuts at: 6555 1606
Size: 2120
Fragments arranged by size:
2120 1254 1148 948 677 461 245 132
84
NaeI GCC'GGC
Cuts at: 2634 2780 2634
Size: 146 6923
NarI GG'CG_CC
Cuts at: 3035 3035
Size: 7069
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6517
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7069
NgoMIV G'CCGG_C
Cuts at: 2632 2778 2632
Size: 146 6923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7069
NotI GC'GGCC_GC
Cuts at: 4516 4516
Size: 7069
NspI r_CATG'y
Cuts at: 1132 1229 4427 5972 1132
Size: 97 3198 1545 2229
Fragments arranged by size:
3198 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2252 1348
Size: 904 6165
PciI A'CATG_T
Cuts at: 5968 5968
Size: 7069
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3331 3639 1108
Size: 2223 308 4538
Fragments arranged by size:
4538 2223 308
PmeI GTTT'AAAC
Cuts at: 4373 4373
Size: 7069
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5138 5170 6651 6683 3728
Size: 32 1378 32 1481 32 4114
Fragments arranged by size:
4114 1481 1378 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2085 2413 2794 2085
Size: 328 381 6360
Fragments arranged by size:
6360 381 328
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7069
PsiI TTA'TAA
Cuts at: 4639 4639
Size: 7069
PspOMI G'GGCC_C
Cuts at: 3302 3302
Size: 7069
PspXI vC'TCGA_Gb
Cuts at: 2810 2810
Size: 7069
PstI C_TGCA'G
Cuts at: 830 2173 2655 3348 3717 4421 830
Size: 1343 482 693 369 704 3478
Fragments arranged by size:
3478 1343 704 693 482 369
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7069
PvuII CAG'CTG
Cuts at: 2860 2944 3076 2860
Size: 84 132 6853
Fragments arranged by size:
6853 132 84
SacI G_AGCT'C
Cuts at: 2307 2934 2307
Size: 627 6442
SalI G'TCGA_C
Cuts at: 1936 4091 4411 1936
Size: 2155 320 4594
Fragments arranged by size:
4594 2155 320
SanDI GG'GwC_CC
Cuts at: 2794 2794
Size: 7069
SapI GCTCTTCn'nnn_
Cuts at: 2667 2909 2667
Size: 242 6827
SbfI CC_TGCA'GG
Cuts at: 4421 4421
Size: 7069
ScaI AGT'ACT
Cuts at: 5317 7029 5317
Size: 1712 5357
SfcI C'TryA_G
Cuts at: 826 1045 2169 2651 3104 3344 3713 4417 5552
Size: 219 1124 482 453 240 369 704 1135
Cuts at: 5552 6233 6424 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1124 704 681 482 453 369
240 219 191
SfoI GGC'GCC
Cuts at: 3036 3036
Size: 7069
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7069
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7069
SmaI CCC'GGG
Cuts at: 1872 2101 3311 4396 1872
Size: 229 1210 1085 4545
Fragments arranged by size:
4545 1210 1085 229
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2306 2810 3978 5173 6074 6336
Size: 197 926 363 504 1168 1195 901 262
Cuts at: 6336 820
Size: 1553
Fragments arranged by size:
1553 1195 1168 926 901 504 363 262
197
SnaBI TAC'GTA
Cuts at: 493 4860 493
Size: 4367 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7069
SphI G_CATG'C
Cuts at: 1132 4427 1132
Size: 3295 3774
SspI AAT'ATT
Cuts at: 5 52 2048 4993 5
Size: 47 1996 2945 2081
Fragments arranged by size:
2945 2081 1996 47
StuI AGG'CCT
Cuts at: 1517 3888 1517
Size: 2371 4698
StyI C'CwwG_G
Cuts at: 513 1065 2973 3465 3633 4508 513
Size: 552 1908 492 168 875 3074
Fragments arranged by size:
3074 1908 875 552 492 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2172 5096 5113 5266 5451 5896 325
Size: 1255 592 2924 17 153 185 445 1498
Fragments arranged by size:
2924 1498 1255 592 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2431 3554 4083
Size: 274 80 33 51 1443 454 1123 529
Cuts at: 4083 4233 5315 7027 96
Size: 150 1082 1712 138
Fragments arranged by size:
1712 1443 1123 1082 529 454 274 150
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2205 2237 3560 3592 3634 3666 3999 4031 7029
Size: 32 1323 32 42 32 333 32 2998
Cuts at: 7029 7061 2205
Size: 32 2213
Fragments arranged by size:
2998 2213 1323 333 42 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2066 2981 1316
Size: 750 915 5404
Fragments arranged by size:
5404 915 750
XbaI T'CTAG_A
Cuts at: 4405 4405
Size: 7069
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3073 3073
Size: 7069
XhoI C'TCGA_G
Cuts at: 1943 2810 3978 1943
Size: 867 1168 5034
Fragments arranged by size:
5034 1168 867
XmaI C'CCGG_G
Cuts at: 1870 2099 3309 4394 1870
Size: 229 1210 1085 4545
Fragments arranged by size:
4545 1210 1085 229
XmnI GAAnn'nnTTC
Cuts at: 5198 5198
Size: 7069
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4877 276
Size: 53 83 186 936 3343 2468
Fragments arranged by size:
3343 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PsrI RsrII SacII SexAI
SfiI SrfI SwaI