Restriction map for
pFN21AB9764
(Circular) (Six-base) MAPSORT of: pFN21AB9764.seq Check: 2057 from: 1 to: 7222
pFN21AB9764 7222 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5032 278
Size: 53 83 186 936 3496 2468
Fragments arranged by size:
3496 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4565 1937
Size: 2628 4594
Acc65I G'GTAC_C
Cuts at: 4543 4543
Size: 7222
AclI AA'CG_TT
Cuts at: 1563 2950 5349 5722 1563
Size: 1387 2399 373 3063
Fragments arranged by size:
3063 2399 1387 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2380 2469 2552 3637 5285 6669 6937 1632
Size: 748 89 83 1085 1648 1384 268 1917
Fragments arranged by size:
1917 1648 1384 1085 748 268 89 83
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7025
AflIII A'CryG_T
Cuts at: 1203 2903 4467 6121 7098 1203
Size: 1700 1564 1654 977 1327
Fragments arranged by size:
1700 1654 1564 1327 977
AgeI A'CCGG_T
Cuts at: 6053 6053
Size: 7222
AhdI GACnn_n'nnGTC
Cuts at: 5951 5951
Size: 7222
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7222
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2123 2155 2123
Size: 32 7190
AlwNI CAG_nnn'CTG
Cuts at: 4965 6537 4965
Size: 1572 5650
ApaLI G'TGCA_C
Cuts at: 5279 6435 5279
Size: 1156 6066
ApoI r'AATT_y
Cuts at: 1493 2051 2288 2613 2821 2989 3015 3073 3099
Size: 558 237 325 208 168 26 58 26
Cuts at: 3099 3241 3267 3577 3603 3745 3771 3855 3997
Size: 142 26 310 26 142 26 84 142
Cuts at: 3997 4249 4275 4359 4516 4531 4757 4909 7018
Size: 252 26 84 157 15 226 152 2109
Cuts at: 7018 1493
Size: 1697
Fragments arranged by size:
2109 1697 558 325 310 252 237 226
208 168 157 152 142 142 142 84
84 58 26 26 26 26 26 26
15
AseI AT'TA_AT
Cuts at: 160 1032 5776 160
Size: 872 4744 1606
Fragments arranged by size:
4744 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7222
AvaI C'yCGr_G
Cuts at: 1870 1943 4547 1870
Size: 73 2604 4545
Fragments arranged by size:
4545 2604 73
BamHI G'GATC_C
Cuts at: 4552 4919 4552
Size: 367 6855
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4543 5998 618
Size: 325 217 627 2756 1455 1842
Fragments arranged by size:
2756 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3157 4541 1391
Size: 1766 1384 4072
Fragments arranged by size:
4072 1766 1384
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3055 3223 3559 3727 3979 4231 928
Size: 971 1156 168 336 168 252 252 3919
Fragments arranged by size:
3919 1156 971 336 252 252 168 168
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5413 5447 1903
Size: 34 3476 34 3678
Fragments arranged by size:
3678 3476 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5114 6324 5114
Size: 1210 6012
BclI T'GATC_A
Cuts at: 1548 2268 1548
Size: 720 6502
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5833 136
Size: 107 122 71 631 605 4161 1525
Fragments arranged by size:
4161 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7217 7217
Size: 7222
BlpI GC'TnA_GC
Cuts at: 3166 4639 3166
Size: 1473 5749
Bme1580I G_kGCm'C
Cuts at: 1790 5283 6439 1790
Size: 3493 1156 2573
Fragments arranged by size:
3493 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2110 1318
Size: 792 6430
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4953 5911 442
Size: 416 53 83 399 24 3536 958 1753
Fragments arranged by size:
3536 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3045 3129 3283 3297 3465 3549 3633 3717
Size: 1806 84 154 14 168 84 84 84
Cuts at: 3717 3801 3885 4053 4137 4305 5882 1239
Size: 84 84 168 84 168 1577 2579
Fragments arranged by size:
2579 1806 1577 168 168 168 154 84
84 84 84 84 84 84 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2447 5347 6212 6510 2447
Size: 2900 865 298 3159
Fragments arranged by size:
3159 2900 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5885 882
Size: 617 4386 2219
Fragments arranged by size:
4386 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5013 493
Size: 711 3809 2702
Fragments arranged by size:
3809 2702 711
BsaBI GATnn'nnATC
Cuts at: 7216 7216
Size: 7222
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5029 5411 6046 275
Size: 53 83 186 936 3496 382 635 1451
Fragments arranged by size:
3496 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3359 4367 5655 6053 6327 6474 6885 3359
Size: 1008 1288 398 274 147 411 3696
Fragments arranged by size:
3696 1288 1008 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2123 2153 1178
Size: 30 165 30 720 30 6247
Fragments arranged by size:
6247 720 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 1186
Size: 892 6330
BseYI C'CCAG_C
Cuts at: 6425 6921 6425
Size: 496 6726
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6649
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4672 5433 6461 1540
Size: 275 186 2671 761 1028 2301
Fragments arranged by size:
2671 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3157 3493 5283 5368 6439 3157
Size: 336 1790 85 1071 3940
Fragments arranged by size:
3940 1790 1071 336 85
BsmI GAATG_Cn'
Cuts at: 3392 4733 4826 3392
Size: 1341 93 5788
Fragments arranged by size:
5788 1341 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3157 3493 4541 5283 5368 6439 1391
Size: 399 1367 336 1048 742 85 1071 2174
Fragments arranged by size:
2174 1367 1071 1048 742 399 336 85
BspEI T'CCGG_A
Cuts at: 6885 6885
Size: 7222
BspHI T'CATG_A
Cuts at: 3382 5111 6841 3382
Size: 1729 1730 3763
Fragments arranged by size:
3763 1730 1729
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4577 844
Size: 3733 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2087 5109 1394
Size: 429 264 3022 3507
Fragments arranged by size:
3507 3022 429 264
BsrDI GCAATG_nn'
Cuts at: 65 1248 4228 5717 5891 65
Size: 1183 2980 1489 174 1396
Fragments arranged by size:
2980 1489 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5866 6053 1700
Size: 110 4056 187 2869
Fragments arranged by size:
4056 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2100 96
Size: 2004 5218
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7222
BssSI C'ACGA_G
Cuts at: 5282 6294 5282
Size: 1012 6210
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7222
BstBI TT'CG_AA
Cuts at: 6026 6026
Size: 7222
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7222
BstXI CCAn_nnnn'nTGG
Cuts at: 2247 4445 2247
Size: 2198 5024
BstYI r'GATC_y
Cuts at: 1972 4552 4919 5304 5321 6773 6859 6871 7217
Size: 2580 367 385 17 1452 86 12 346
Cuts at: 7217 1972
Size: 1977
Fragments arranged by size:
2580 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5731
Fragments arranged by size:
5731 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6483
Fragments arranged by size:
6483 683 56
BtsI GCAGTG_nn'
Cuts at: 4738 5532 5552 4738
Size: 794 20 6408
Fragments arranged by size:
6408 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7222
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4210 4245 529
Size: 35 3646 35 3506
Fragments arranged by size:
3646 3506 35 35
DraI TTT'AAA
Cuts at: 4526 4526
Size: 7222
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6229 809
Size: 507 4913 1802
Fragments arranged by size:
4913 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4669 5558 7187
Size: 54 1425 50 275 18 2839 889 1629
Cuts at: 7187 8
Size: 43
Fragments arranged by size:
2839 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4669 1537
Size: 275 2857 4090
Fragments arranged by size:
4090 2857 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2287 2315 2497 2740 5152 1353
Size: 934 28 182 243 2412 3423
Fragments arranged by size:
3423 2412 934 243 182 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5809 6183 6329 387
Size: 1064 138 4220 374 146 1280
Fragments arranged by size:
4220 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3155 3155
Size: 7222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2380 2469 2552 3045 3129 3283 3297
Size: 393 748 89 83 493 84 154 14
Cuts at: 3297 3465 3549 3633 3637 3717 3801 3885 4053
Size: 168 84 84 4 80 84 84 168
Cuts at: 4053 4137 4305 5285 5882 6669 6937 1239
Size: 84 168 980 597 787 268 1524
Fragments arranged by size:
1524 980 787 748 597 493 393 268
168 168 168 154 89 84 84 84
84 84 84 83 80 14 4
EcoRI G'AATT_C
Cuts at: 2051 3073 3099 3267 3603 3745 3771 3855 3997
Size: 1022 26 168 336 142 26 84 142
Cuts at: 3997 4249 4275 4359 4516 4531 4909 2051
Size: 252 26 84 157 15 378 4364
Fragments arranged by size:
4364 1022 378 336 252 168 157 142
142 84 84 26 26 26 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2486 2486
Size: 7222
FspI TGC'GCA
Cuts at: 5728 5728
Size: 7222
HaeII r_GCGC'y
Cuts at: 1678 6369 1678
Size: 4691 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2260 2292 2717 2749 5863
Size: 32 135 32 688 32 425 32 3114
Cuts at: 5863 5895 5937 5969 1373
Size: 32 42 32 2626
Fragments arranged by size:
3114 2626 688 425 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4566 4812 669
Size: 988 281 2628 246 3079
Fragments arranged by size:
3079 2628 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2537 3163 4088 748
Size: 1789 626 925 3882
Fragments arranged by size:
3882 1789 925 626
HpaI GTT'AAC
Cuts at: 4812 4812
Size: 7222
KpnI G_GTAC'C
Cuts at: 4547 4547
Size: 7222
MfeI C'AATT_G
Cuts at: 4821 4821
Size: 7222
MluI A'CGCG_T
Cuts at: 7098 7098
Size: 7222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2097 2163 2337 6336 6520 1305
Size: 792 66 174 3999 184 2007
Fragments arranged by size:
3999 2007 792 184 174 66
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5400
Fragments arranged by size:
5400 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3948 4132 4384 4443 5180 5539 5698
Size: 648 2782 184 252 59 737 359 159
Cuts at: 5698 518
Size: 2042
Fragments arranged by size:
2782 2042 737 648 359 252 184 159
59
MspA1I CmG'CkG
Cuts at: 1606 4638 5315 6463 6708 1606
Size: 3032 677 1148 245 2120
Fragments arranged by size:
3032 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5731
Fragments arranged by size:
5731 939 552
NdeI CA'TA_TG
Cuts at: 387 4129 4381 387
Size: 3742 252 3228
Fragments arranged by size:
3742 3228 252
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7222
NotI GC'GGCC_GC
Cuts at: 4669 4669
Size: 7222
NspI r_CATG'y
Cuts at: 1132 1229 2076 4580 6125 1132
Size: 97 847 2504 1545 2229
Fragments arranged by size:
2504 2229 1545 847 97
PasI CC'CwG_GG
Cuts at: 1348 2017 2143 1348
Size: 669 126 6427
Fragments arranged by size:
6427 669 126
PciI A'CATG_T
Cuts at: 6121 6121
Size: 7222
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7222
PmeI GTTT'AAAC
Cuts at: 4526 4526
Size: 7222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2123 2155 5291 5323 6804 6836 2123
Size: 32 3136 32 1481 32 2509
Fragments arranged by size:
3136 2509 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7222
PsiI TTA'TAA
Cuts at: 2688 3039 3459 3795 4792 2688
Size: 351 420 336 997 5118
Fragments arranged by size:
5118 997 420 351 336
PstI C_TGCA'G
Cuts at: 830 2592 4574 830
Size: 1762 1982 3478
Fragments arranged by size:
3478 1982 1762
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7222
SacI G_AGCT'C
Cuts at: 3157 3157
Size: 7222
SalI G'TCGA_C
Cuts at: 1936 4564 1936
Size: 2628 4594
SbfI CC_TGCA'GG
Cuts at: 4574 4574
Size: 7222
ScaI AGT'ACT
Cuts at: 5470 7182 5470
Size: 1712 5510
SfcI C'TryA_G
Cuts at: 826 1045 2046 2588 4570 5705 6386 6577 826
Size: 219 1001 542 1982 1135 681 191 1471
Fragments arranged by size:
1982 1471 1135 1001 681 542 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7222
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7222
SmaI CCC'GGG
Cuts at: 1872 4549 1872
Size: 2677 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2426 5326 6227 6489 820
Size: 197 926 483 2900 901 262 1553
Fragments arranged by size:
2900 1553 926 901 483 262 197
SnaBI TAC'GTA
Cuts at: 493 5013 493
Size: 4520 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7222
SphI G_CATG'C
Cuts at: 1132 4580 1132
Size: 3448 3774
SspI AAT'ATT
Cuts at: 5 52 5146 5
Size: 47 5094 2081
Fragments arranged by size:
5094 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7222
StyI C'CwwG_G
Cuts at: 513 1065 2004 4661 513
Size: 552 939 2657 3074
Fragments arranged by size:
3074 2657 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2030 5249 5266 5419 5604 6049 325
Size: 1255 450 3219 17 153 185 445 1498
Fragments arranged by size:
3219 1498 1255 450 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2100 2221 5468
Size: 274 80 33 51 1443 123 121 3247
Cuts at: 5468 7180 96
Size: 1712 138
Fragments arranged by size:
3247 1712 1443 274 138 123 121 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7182 7214 7182
Size: 32 7190
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7222
XbaI T'CTAG_A
Cuts at: 4558 4558
Size: 7222
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7222
XmaI C'CCGG_G
Cuts at: 1870 4547 1870
Size: 2677 4545
XmnI GAAnn'nnTTC
Cuts at: 2642 2905 4238 5351 2642
Size: 263 1333 1113 4513
Fragments arranged by size:
4513 1333 1113 263
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5030 276
Size: 53 83 186 936 3496 2468
Fragments arranged by size:
3496 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI