Restriction map for
pFN21AB9762
(Circular) (Six-base) MAPSORT of: pFN21AB9762.seq Check: 9575 from: 1 to: 7105
pFN21AB9762 7105 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3972 4915 278
Size: 53 83 186 936 2436 943 2468
Fragments arranged by size:
2468 2436 943 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2760 3642 4448 1937
Size: 823 882 806 4594
Fragments arranged by size:
4594 882 823 806
Acc65I G'GTAC_C
Cuts at: 3154 4426 3154
Size: 1272 5833
AclI AA'CG_TT
Cuts at: 1563 2154 5232 5605 1563
Size: 591 3078 373 3063
Fragments arranged by size:
3078 3063 591 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2951 3640 3672 5168 6552 6820 1632
Size: 1319 689 32 1496 1384 268 1917
Fragments arranged by size:
1917 1496 1384 1319 689 268 32
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6908
AflIII A'CryG_T
Cuts at: 1203 2742 6004 6981 1203
Size: 1539 3262 977 1327
Fragments arranged by size:
3262 1539 1327 977
AgeI A'CCGG_T
Cuts at: 5936 5936
Size: 7105
AhdI GACnn_n'nnGTC
Cuts at: 5834 5834
Size: 7105
AleI CACnn'nnGTG
Cuts at: 1166 3428 1166
Size: 2262 4843
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7105
AlwNI CAG_nnn'CTG
Cuts at: 2546 4271 4848 6420 2546
Size: 1725 577 1572 3231
Fragments arranged by size:
3231 1725 1572 577
ApaLI G'TGCA_C
Cuts at: 2738 5162 6318 2738
Size: 2424 1156 3525
Fragments arranged by size:
3525 2424 1156
ApoI r'AATT_y
Cuts at: 1493 2915 3916 4176 4282 4294 4414 4640 4792
Size: 1422 1001 260 106 12 120 226 152
Cuts at: 4792 6901 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1422 1001 260 226 152 120
106 12
AseI AT'TA_AT
Cuts at: 160 1032 5659 160
Size: 872 4627 1606
Fragments arranged by size:
4627 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7105
AvaI C'yCGr_G
Cuts at: 1870 1943 2401 4430 1870
Size: 73 458 2029 4545
Fragments arranged by size:
4545 2029 458 73
BamHI G'GATC_C
Cuts at: 3236 4435 4802 3236
Size: 1199 367 5539
Fragments arranged by size:
5539 1199 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2245 3154 4426 5881 618
Size: 325 217 627 458 909 1272 1455 1842
Fragments arranged by size:
1842 1455 1272 909 627 458 325 217
BanII G_rGCy'C
Cuts at: 1391 2393 2401 3941 4424 1391
Size: 1002 8 1540 483 4072
Fragments arranged by size:
4072 1540 1002 483 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2589 928
Size: 971 690 5444
Fragments arranged by size:
5444 971 690
BbvCI CC'TCA_GC
Cuts at: 2427 2427
Size: 7105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5296 5330 1903
Size: 34 3359 34 3678
Fragments arranged by size:
3678 3359 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4997 6207 4997
Size: 1210 5895
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7105
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2139 2345
Size: 107 122 71 631 605 337 130 206
Cuts at: 2345 5716 136
Size: 3371 1525
Fragments arranged by size:
3371 1525 631 605 337 206 130 122
107 71
BglII A'GATC_T
Cuts at: 3538 7100 3538
Size: 3562 3543
BlpI GC'TnA_GC
Cuts at: 4522 4522
Size: 7105
Bme1580I G_kGCm'C
Cuts at: 1790 2250 2501 2742 5166 6322 1790
Size: 460 251 241 2424 1156 2573
Fragments arranged by size:
2573 2424 1156 460 251 241
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7105
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4281 4836 5794
Size: 416 53 83 399 24 2864 555 958
Cuts at: 5794 442
Size: 1753
Fragments arranged by size:
2864 1753 958 555 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2393 3483 5765 1239
Size: 1154 1090 2282 2579
Fragments arranged by size:
2579 2282 1154 1090
Bpu10I CC'TnA_GC
Cuts at: 2427 2427
Size: 7105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5230 6095 6393 5230
Size: 865 298 5942
Fragments arranged by size:
5942 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2882 3425 4241 5768 882
Size: 617 1383 543 816 1527 2219
Fragments arranged by size:
2219 1527 1383 816 617 543
BsaAI yAC'GTr
Cuts at: 493 1204 4896 493
Size: 711 3692 2702
Fragments arranged by size:
3692 2702 711
BsaBI GATnn'nnATC
Cuts at: 7099 7099
Size: 7105
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2162 2265 3969 4912
Size: 53 83 186 936 629 103 1704 943
Cuts at: 4912 5294 5929 275
Size: 382 635 1451
Fragments arranged by size:
1704 1451 943 936 635 629 382 186
103 83 53
BsaWI w'CCGG_w
Cuts at: 2260 2393 3661 5538 5936 6210 6357 6768 2260
Size: 133 1268 1877 398 274 147 411 2597
Fragments arranged by size:
2597 1877 1268 411 398 274 147 133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4021 4051 4295 4325 1178
Size: 30 165 30 2618 30 244 30 3958
Fragments arranged by size:
3958 2618 244 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2390 1186
Size: 850 354 5901
Fragments arranged by size:
5901 850 354
BseYI C'CCAG_C
Cuts at: 6308 6804 6308
Size: 496 6609
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6532
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2523 4555 5316 6344 1540
Size: 275 186 522 2032 761 1028 2301
Fragments arranged by size:
2301 2032 1028 761 522 275 186
BsiHKAI G_wGCw'C
Cuts at: 2742 3012 5166 5251 6322 2742
Size: 270 2154 85 1071 3525
Fragments arranged by size:
3525 2154 1071 270 85
BsmI GAATG_Cn'
Cuts at: 4616 4709 4616
Size: 93 7012
BsmBI CGTCTCn'nnnn_
Cuts at: 2530 2530
Size: 7105
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2250 2393 2401 2501 2742 3012 3941
Size: 399 460 143 8 100 241 270 929
Cuts at: 3941 4424 5166 5251 6322 1391
Size: 483 742 85 1071 2174
Fragments arranged by size:
2174 1071 929 742 483 460 399 270
241 143 100 85 8
BspEI T'CCGG_A
Cuts at: 2260 2393 3661 6768 2260
Size: 133 1268 3107 2597
Fragments arranged by size:
3107 2597 1268 133
BspHI T'CATG_A
Cuts at: 4994 6724 4994
Size: 1730 5375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4460 844
Size: 3616 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4992 1394
Size: 429 3169 3507
Fragments arranged by size:
3507 3169 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5600 5774 65
Size: 1183 4352 174 1396
Fragments arranged by size:
4352 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2267 2420 3875 5749 5936 1700
Size: 110 457 153 1455 1874 187 2869
Fragments arranged by size:
2869 1874 1455 457 187 153 110
BsrGI T'GTAC_A
Cuts at: 96 4345 96
Size: 4249 2856
BssHII G'CGCG_C
Cuts at: 1925 2439 1925
Size: 514 6591
BssSI C'ACGA_G
Cuts at: 5165 6177 5165
Size: 1012 6093
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7105
BstBI TT'CG_AA
Cuts at: 2905 5909 2905
Size: 3004 4101
BstEII G'GTnAC_C
Cuts at: 1184 2431 2455 1184
Size: 1247 24 5834
Fragments arranged by size:
5834 1247 24
BstYI r'GATC_y
Cuts at: 1972 2632 3236 3256 3538 3993 4435 4802 5187
Size: 660 604 20 282 455 442 367 385
Cuts at: 5187 5204 6656 6742 6754 7100 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 660 604 455 442 385 367
346 282 86 20 17 12
Bsu36I CC'TnA_GG
Cuts at: 2055 2539 2055
Size: 484 6621
BtgI C'CryG_G
Cuts at: 513 1065 2004 2202 2517 2806 513
Size: 552 939 198 315 289 4812
Fragments arranged by size:
4812 939 552 315 289 198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3080 487
Size: 683 56 1854 4512
Fragments arranged by size:
4512 1854 683 56
BtsI GCAGTG_nn'
Cuts at: 2388 3423 3435 4621 5415 5435 2388
Size: 1035 12 1186 794 20 4058
Fragments arranged by size:
4058 1186 1035 794 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7105
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7070
DraI TTT'AAA
Cuts at: 4409 4409
Size: 7105
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6112 809
Size: 507 4796 1802
Fragments arranged by size:
4796 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2298 3877 4552
Size: 54 1425 50 275 18 468 1579 675
Cuts at: 4552 5441 7070 8
Size: 889 1629 43
Fragments arranged by size:
1629 1579 1425 889 675 468 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4552 1537
Size: 275 2740 4090
Fragments arranged by size:
4090 2740 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2337 2951 3171 3294 3691 5035 1353
Size: 984 614 220 123 397 1344 3423
Fragments arranged by size:
3423 1344 984 614 397 220 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2033 2206 5692 6066 6212 387
Size: 1064 138 444 173 3486 374 146 1280
Fragments arranged by size:
3486 1280 1064 444 374 173 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2393 2951 3483 3640 3672 5168 5765
Size: 393 761 558 532 157 32 1496 597
Cuts at: 5765 6552 6820 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1496 787 761 597 558 532 393
268 157 32
EcoO109I rG'GnC_Cy
Cuts at: 2190 2543 2190
Size: 353 6752
EcoRI G'AATT_C
Cuts at: 4294 4414 4792 4294
Size: 120 378 6607
Fragments arranged by size:
6607 378 120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2809 2809
Size: 7105
FspI TGC'GCA
Cuts at: 5611 5611
Size: 7105
HaeII r_GCGC'y
Cuts at: 1678 2015 2274 2351 2421 2427 2508 6252 1678
Size: 337 259 77 70 6 81 3744 2531
Fragments arranged by size:
3744 2531 337 259 81 77 70 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3545 3577 4021 4053 5746
Size: 32 135 32 1973 32 444 32 1693
Cuts at: 5746 5778 5820 5852 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1973 1693 444 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4449 4695 669
Size: 988 281 2511 246 3079
Fragments arranged by size:
3079 2511 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3512 748
Size: 2764 4341
HpaI GTT'AAC
Cuts at: 4695 4695
Size: 7105
KpnI G_GTAC'C
Cuts at: 3158 4430 3158
Size: 1272 5833
MfeI C'AATT_G
Cuts at: 4704 4704
Size: 7105
MluI A'CGCG_T
Cuts at: 6981 6981
Size: 7105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2545 2877 3297 4096 6219 6403 1305
Size: 1240 332 420 799 2123 184 2007
Fragments arranged by size:
2123 2007 1240 799 420 332 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5283
Fragments arranged by size:
5283 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2775 3428 5063 5422 5581 518
Size: 648 1609 653 1635 359 159 2042
Fragments arranged by size:
2042 1635 1609 653 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2222 2255 4521 5198 6346 6591 1606
Size: 616 33 2266 677 1148 245 2120
Fragments arranged by size:
2266 2120 1148 677 616 245 33
NaeI GCC'GGC
Cuts at: 2269 2422 3877 2269
Size: 153 1455 5497
Fragments arranged by size:
5497 1455 153
NcoI C'CATG_G
Cuts at: 513 1065 2004 2806 513
Size: 552 939 802 4812
Fragments arranged by size:
4812 939 802 552
NdeI CA'TA_TG
Cuts at: 387 3790 387
Size: 3403 3702
NgoMIV G'CCGG_C
Cuts at: 2267 2420 3875 2267
Size: 153 1455 5497
Fragments arranged by size:
5497 1455 153
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7105
NotI GC'GGCC_GC
Cuts at: 4552 4552
Size: 7105
NspI r_CATG'y
Cuts at: 1132 1229 2746 4463 6008 1132
Size: 97 1517 1717 1545 2229
Fragments arranged by size:
2229 1717 1545 1517 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7105
PciI A'CATG_T
Cuts at: 2742 6004 2742
Size: 3262 3843
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7105
PmeI GTTT'AAAC
Cuts at: 4409 4409
Size: 7105
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5174 5206 6687 6719 5174
Size: 32 1481 32 5560
Fragments arranged by size:
5560 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2190 2543 2190
Size: 353 6752
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7105
PsiI TTA'TAA
Cuts at: 4675 4675
Size: 7105
PstI C_TGCA'G
Cuts at: 830 2310 3430 3946 4387 4457 830
Size: 1480 1120 516 441 70 3478
Fragments arranged by size:
3478 1480 1120 516 441 70
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7105
SalI G'TCGA_C
Cuts at: 1936 4447 1936
Size: 2511 4594
SanDI GG'GwC_CC
Cuts at: 2190 2190
Size: 7105
SbfI CC_TGCA'GG
Cuts at: 4457 4457
Size: 7105
ScaI AGT'ACT
Cuts at: 5353 7065 5353
Size: 1712 5393
SexAI A'CCwGG_T
Cuts at: 4371 4371
Size: 7105
SfcI C'TryA_G
Cuts at: 826 1045 2306 2656 3426 3843 3942 4059 4263
Size: 219 1261 350 770 417 99 117 204
Cuts at: 4263 4383 4453 5588 6269 6460 826
Size: 120 70 1135 681 191 1471
Fragments arranged by size:
1471 1261 1135 770 681 417 350 219
204 191 120 117 99 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7105
SgrAI Cr'CCGG_yG
Cuts at: 1700 2267 2420 1700
Size: 567 153 6385
Fragments arranged by size:
6385 567 153
SmaI CCC'GGG
Cuts at: 1872 2403 4432 1872
Size: 531 2029 4545
Fragments arranged by size:
4545 2029 531
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5209 6110 6372 820
Size: 197 926 3266 901 262 1553
Fragments arranged by size:
3266 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4896 493
Size: 4403 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7105
SphI G_CATG'C
Cuts at: 1132 4463 1132
Size: 3331 3774
SspI AAT'ATT
Cuts at: 5 52 3870 5029 5
Size: 47 3818 1159 2081
Fragments arranged by size:
3818 2081 1159 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7105
StyI C'CwwG_G
Cuts at: 513 1065 2004 2806 3361 4544 513
Size: 552 939 802 555 1183 3074
Fragments arranged by size:
3074 1183 939 802 555 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2298 3520 3837 4265 5132 5149 5302
Size: 1255 718 1222 317 428 867 17 153
Cuts at: 5302 5487 5932 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1222 867 718 445 428 317
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2745 4345 5351
Size: 274 80 33 51 1443 768 1600 1006
Cuts at: 5351 7063 96
Size: 1712 138
Fragments arranged by size:
1712 1600 1443 1006 768 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7065 7097 7065
Size: 32 7073
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7105
XbaI T'CTAG_A
Cuts at: 4441 4441
Size: 7105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2203 2011
Size: 192 6913
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7105
XmaI C'CCGG_G
Cuts at: 1870 2401 4430 1870
Size: 531 2029 4545
Fragments arranged by size:
4545 2029 531
XmnI GAAnn'nnTTC
Cuts at: 2672 5234 2672
Size: 2562 4543
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3970 4913 276
Size: 53 83 186 936 2436 943 2468
Fragments arranged by size:
2468 2436 943 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SapI SfiI SfoI SrfI SwaI