Restriction map for
pFN21AB9761
(Circular) (Six-base) MAPSORT of: pFN21AB9761.seq Check: 9855 from: 1 to: 7003
pFN21AB9761 7003 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2841 2841
Size: 7003
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4813 278
Size: 53 83 186 936 3277 2468
Fragments arranged by size:
3277 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4346 1937
Size: 2409 4594
Acc65I G'GTAC_C
Cuts at: 4324 4324
Size: 7003
AclI AA'CG_TT
Cuts at: 1563 2229 5130 5503 1563
Size: 666 2901 373 3063
Fragments arranged by size:
3063 2901 666 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3540 5066 6450 6718 1632
Size: 1908 1526 1384 268 1917
Fragments arranged by size:
1917 1908 1526 1384 268
AfeI AGC'GCT
Cuts at: 2477 2477
Size: 7003
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6806
AflIII A'CryG_T
Cuts at: 1203 2595 5902 6879 1203
Size: 1392 3307 977 1327
Fragments arranged by size:
3307 1392 1327 977
AgeI A'CCGG_T
Cuts at: 5834 5834
Size: 7003
AhdI GACnn_n'nnGTC
Cuts at: 3622 5732 3622
Size: 2110 4893
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7003
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7003
AlwNI CAG_nnn'CTG
Cuts at: 2266 2782 3262 3620 4746 6318 2266
Size: 516 480 358 1126 1572 2951
Fragments arranged by size:
2951 1572 1126 516 480 358
ApaLI G'TGCA_C
Cuts at: 2810 2945 5060 6216 2810
Size: 135 2115 1156 3597
Fragments arranged by size:
3597 2115 1156 135
ApoI r'AATT_y
Cuts at: 1493 2060 4312 4538 4690 6799 1493
Size: 567 2252 226 152 2109 1697
Fragments arranged by size:
2252 2109 1697 567 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3789 5557 160
Size: 872 2757 1768 1606
Fragments arranged by size:
2757 1768 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7003
AvaI C'yCGr_G
Cuts at: 1870 1943 3364 4328 1870
Size: 73 1421 964 4545
Fragments arranged by size:
4545 1421 964 73
BamHI G'GATC_C
Cuts at: 3567 4333 4700 3567
Size: 766 367 5870
Fragments arranged by size:
5870 766 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4324 5779 618
Size: 325 217 627 2537 1455 1842
Fragments arranged by size:
2537 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2184 2249 3468 3479 3932 4322 1391
Size: 793 65 1219 11 453 390 4072
Fragments arranged by size:
4072 1219 793 453 390 65 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2798 928
Size: 971 899 5133
Fragments arranged by size:
5133 971 899
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2926 2936 2960 2970 5194 5228 1903
Size: 34 989 10 24 10 2224 34 3678
Fragments arranged by size:
3678 2224 989 34 34 24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 4895 6105 4895
Size: 1210 5793
BclI T'GATC_A
Cuts at: 1548 3205 1548
Size: 1657 5346
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3222 5614 136
Size: 107 122 71 631 605 1550 2392 1525
Fragments arranged by size:
2392 1550 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3042 6998 3042
Size: 3956 3047
BlpI GC'TnA_GC
Cuts at: 4420 4420
Size: 7003
Bme1580I G_kGCm'C
Cuts at: 1790 2814 2949 5064 6220 1790
Size: 1024 135 2115 1156 2573
Fragments arranged by size:
2573 2115 1156 1024 135
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7003
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2252 4734 5692
Size: 416 53 83 399 24 835 2482 958
Cuts at: 5692 442
Size: 1753
Fragments arranged by size:
2482 1753 958 835 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7003
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 7003
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2243 2728 2947 3115 3597 4178 5663 1239
Size: 1004 485 219 168 482 581 1485 2579
Fragments arranged by size:
2579 1485 1004 581 485 482 219 168
Bpu10I CC'TnA_GC
Cuts at: 3755 3755
Size: 7003
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2188 5128 5993 6291 2188
Size: 2940 865 298 2900
Fragments arranged by size:
2940 2900 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4065 5666 882
Size: 617 2566 1601 2219
Fragments arranged by size:
2566 2219 1601 617
BsaAI yAC'GTr
Cuts at: 493 1204 4794 493
Size: 711 3590 2702
Fragments arranged by size:
3590 2702 711
BsaBI GATnn'nnATC
Cuts at: 3199 6997 3199
Size: 3798 3205
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4810 5192 5827 275
Size: 53 83 186 936 3277 382 635 1451
Fragments arranged by size:
3277 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2363 5436 5834 6108 6255 6666 2363
Size: 3073 398 274 147 411 2700
Fragments arranged by size:
3073 2700 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2942 2972 3235 3265 3455
Size: 30 165 30 1539 30 263 30 190
Cuts at: 3455 3485 4072 4102 1178
Size: 30 587 30 4079
Fragments arranged by size:
4079 1539 587 263 190 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2950 4044 1186
Size: 1764 1094 4145
Fragments arranged by size:
4145 1764 1094
BseYI C'CCAG_C
Cuts at: 2660 2913 3288 3686 3797 4255 6206 6702 2660
Size: 253 375 398 111 458 1951 496 2961
Fragments arranged by size:
2961 1951 496 458 398 375 253 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2477 2945 1163
Size: 573 741 468 5221
Fragments arranged by size:
5221 741 573 468
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4453 5214 6242 1540
Size: 275 186 2452 761 1028 2301
Fragments arranged by size:
2452 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2424 2814 2949 4164 5064 5149 6220 2424
Size: 390 135 1215 900 85 1071 3207
Fragments arranged by size:
3207 1215 1071 900 390 135 85
BsmI GAATG_Cn'
Cuts at: 4039 4514 4607 4039
Size: 475 93 6435
Fragments arranged by size:
6435 475 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2184 2249 2424 2814 2949 3468 3479
Size: 399 394 65 175 390 135 519 11
Cuts at: 3479 3932 4164 4322 5064 5149 6220 1391
Size: 453 232 158 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 519 453 399 394 390
232 175 158 135 85 65 11
BspEI T'CCGG_A
Cuts at: 6666 6666
Size: 7003
BspHI T'CATG_A
Cuts at: 3208 4892 6622 3208
Size: 1684 1730 3589
Fragments arranged by size:
3589 1730 1684
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2841 3420 4358 844
Size: 1997 579 938 3489
Fragments arranged by size:
3489 1997 938 579
BsrBI CCG'CTC
Cuts at: 1394 1823 4890 1394
Size: 429 3067 3507
Fragments arranged by size:
3507 3067 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5498 5672 65
Size: 1183 4250 174 1396
Fragments arranged by size:
4250 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2522 4110 5647 5834 1700
Size: 110 712 1588 1537 187 2869
Fragments arranged by size:
2869 1588 1537 712 187 110
BsrGI T'GTAC_A
Cuts at: 96 2310 96
Size: 2214 4789
BssHII G'CGCG_C
Cuts at: 1925 2841 1925
Size: 916 6087
BssSI C'ACGA_G
Cuts at: 5063 6075 5063
Size: 1012 5991
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2851 794
Size: 2057 4946
BstBI TT'CG_AA
Cuts at: 5807 5807
Size: 7003
BstEII G'GTnAC_C
Cuts at: 1184 2872 3641 1184
Size: 1688 769 4546
Fragments arranged by size:
4546 1688 769
BstXI CCAn_nnnn'nTGG
Cuts at: 2221 2221
Size: 7003
BstYI r'GATC_y
Cuts at: 1972 2574 3042 3567 4333 4700 5085 5102 6554
Size: 602 468 525 766 367 385 17 1452
Cuts at: 6554 6640 6652 6998 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 766 602 525 468 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2489 2775 2489
Size: 286 6717
BtgI C'CryG_G
Cuts at: 513 1065 2640 3571 3862 513
Size: 552 1575 931 291 3654
Fragments arranged by size:
3654 1575 931 552 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3535 487
Size: 683 56 2309 3955
Fragments arranged by size:
3955 2309 683 56
BtsI GCAGTG_nn'
Cuts at: 2554 4519 5313 5333 2554
Size: 1965 794 20 4224
Fragments arranged by size:
4224 1965 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7003
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6968
DraI TTT'AAA
Cuts at: 4307 4307
Size: 7003
DraIII CAC_nnn'GTG
Cuts at: 2455 2809 3893 2455
Size: 354 1084 5565
Fragments arranged by size:
5565 1084 354
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6010 809
Size: 507 4694 1802
Fragments arranged by size:
4694 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2233 2508 2637
Size: 54 1425 50 275 18 403 275 129
Cuts at: 2637 3627 4450 5339 6968 8
Size: 990 823 889 1629 43
Fragments arranged by size:
1629 1425 990 889 823 403 275 275
129 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4450 1537
Size: 275 2638 4090
Fragments arranged by size:
4090 2638 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3372 3732 4933 1353
Size: 2019 360 1201 3423
Fragments arranged by size:
3423 2019 1201 360
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2567 2768 3240 5590 5964 6110
Size: 1064 138 978 201 472 2350 374 146
Cuts at: 6110 387
Size: 1280
Fragments arranged by size:
2350 1280 1064 978 472 374 201 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 2728 2947 3115 3540 3597 4178
Size: 393 611 485 219 168 425 57 581
Cuts at: 4178 5066 5663 6450 6718 1239
Size: 888 597 787 268 1524
Fragments arranged by size:
1524 888 787 611 597 581 485 425
393 268 219 168 57
EcoNI CCTnn'n_nnAGG
Cuts at: 3500 3500
Size: 7003
EcoO109I rG'GnC_Cy
Cuts at: 2779 2779
Size: 7003
EcoRI G'AATT_C
Cuts at: 4312 4690 4312
Size: 378 6625
EcoRV GAT'ATC
Cuts at: 3650 3650
Size: 7003
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2521 2749 2521
Size: 228 6775
FspI TGC'GCA
Cuts at: 5509 5509
Size: 7003
HaeII r_GCGC'y
Cuts at: 1678 2479 2667 6150 1678
Size: 801 188 3483 2531
Fragments arranged by size:
3483 2531 801 188
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2108 2134 2140 2166 3029
Size: 32 135 32 536 26 6 26 863
Cuts at: 3029 3061 3233 3265 3317 3349 3608 3640 3967
Size: 32 172 32 52 32 259 32 327
Cuts at: 3967 3999 5644 5676 5718 5750 1373
Size: 32 1645 32 42 32 2626
Fragments arranged by size:
2626 1645 863 536 327 259 172 135
52 42 32 32 32 32 32 32
32 32 32 26 26 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2989 4347 4593 669
Size: 988 281 1051 1358 246 3079
Fragments arranged by size:
3079 1358 1051 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7003
HpaI GTT'AAC
Cuts at: 4593 4593
Size: 7003
KpnI G_GTAC'C
Cuts at: 4328 4328
Size: 7003
MfeI C'AATT_G
Cuts at: 4602 4602
Size: 7003
MluI A'CGCG_T
Cuts at: 6879 6879
Size: 7003
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2189 6117 6301 1305
Size: 884 3928 184 2007
Fragments arranged by size:
3928 2007 884 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2510 10
Size: 54 1425 343 678 4503
Fragments arranged by size:
4503 1425 678 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2282 3588 3939 4167 4221 4961 5320
Size: 648 1116 1306 351 228 54 740 359
Cuts at: 5320 5479 518
Size: 159 2042
Fragments arranged by size:
2042 1306 1116 740 648 359 351 228
159 54
MspA1I CmG'CkG
Cuts at: 1606 2113 2562 4419 5096 6244 6489 1606
Size: 507 449 1857 677 1148 245 2120
Fragments arranged by size:
2120 1857 1148 677 507 449 245
NaeI GCC'GGC
Cuts at: 2524 2524
Size: 7003
NcoI C'CATG_G
Cuts at: 513 1065 3571 3862 513
Size: 552 2506 291 3654
Fragments arranged by size:
3654 2506 552 291
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7003
NgoMIV G'CCGG_C
Cuts at: 2522 2522
Size: 7003
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7003
NotI GC'GGCC_GC
Cuts at: 4450 4450
Size: 7003
NspI r_CATG'y
Cuts at: 1132 1229 2599 3544 4133 4361 5906 1132
Size: 97 1370 945 589 228 1545 2229
Fragments arranged by size:
2229 1545 1370 945 589 228 97
PasI CC'CwG_GG
Cuts at: 1348 3620 1348
Size: 2272 4731
PciI A'CATG_T
Cuts at: 2595 5902 2595
Size: 3307 3696
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2266 2626 3620 1108
Size: 1158 360 994 4491
Fragments arranged by size:
4491 1158 994 360
PfoI T'CCnGG_A
Cuts at: 3149 3149
Size: 7003
PmeI GTTT'AAAC
Cuts at: 4307 4307
Size: 7003
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5072 5104 6585 6617 5072
Size: 32 1481 32 5458
Fragments arranged by size:
5458 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2779 2779
Size: 7003
PshAI GACnn'nnGTC
Cuts at: 1613 3954 1613
Size: 2341 4662
PsiI TTA'TAA
Cuts at: 3707 4286 4573 3707
Size: 579 287 6137
Fragments arranged by size:
6137 579 287
PspXI vC'TCGA_Gb
Cuts at: 3364 3364
Size: 7003
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2876 2908 2876
Size: 32 6971
PstI C_TGCA'G
Cuts at: 830 2561 3027 4355 830
Size: 1731 466 1328 3478
Fragments arranged by size:
3478 1731 1328 466
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7003
PvuII CAG'CTG
Cuts at: 2113 2113
Size: 7003
RsrII CG'GwC_CG
Cuts at: 3325 3325
Size: 7003
SalI G'TCGA_C
Cuts at: 1936 4345 1936
Size: 2409 4594
SbfI CC_TGCA'GG
Cuts at: 4355 4355
Size: 7003
ScaI AGT'ACT
Cuts at: 2885 5251 6963 2885
Size: 2366 1712 2925
Fragments arranged by size:
2925 2366 1712
SfcI C'TryA_G
Cuts at: 826 1045 2557 2887 3023 3310 4351 5486 6167
Size: 219 1512 330 136 287 1041 1135 681
Cuts at: 6167 6358 826
Size: 191 1471
Fragments arranged by size:
1512 1471 1135 1041 681 330 287 219
191 136
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7003
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7003
SmaI CCC'GGG
Cuts at: 1872 4330 1872
Size: 2458 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2203 3364 5107 6008 6270 820
Size: 197 926 260 1161 1743 901 262 1553
Fragments arranged by size:
1743 1553 1161 926 901 262 260 197
SnaBI TAC'GTA
Cuts at: 493 4794 493
Size: 4301 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7003
SphI G_CATG'C
Cuts at: 1132 3544 4133 4361 1132
Size: 2412 589 228 3774
Fragments arranged by size:
3774 2412 589 228
SspI AAT'ATT
Cuts at: 5 52 4927 5
Size: 47 4875 2081
Fragments arranged by size:
4875 2081 47
StuI AGG'CCT
Cuts at: 1517 3280 3401 3506 1517
Size: 1763 121 105 5014
Fragments arranged by size:
5014 1763 121 105
StyI C'CwwG_G
Cuts at: 513 1065 3571 3862 4197 4442 513
Size: 552 2506 291 335 245 3074
Fragments arranged by size:
3074 2506 552 335 291 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2911 3254 3660 5030 5047 5200 5385
Size: 1255 1331 343 406 1370 17 153 185
Cuts at: 5385 5830 325
Size: 445 1498
Fragments arranged by size:
1498 1370 1331 1255 445 406 343 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2310 2583 2883
Size: 274 80 33 51 1443 333 273 300
Cuts at: 2883 5249 6961 96
Size: 2366 1712 138
Fragments arranged by size:
2366 1712 1443 333 300 274 273 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3235 3267 3455 3487 6963 6995 3235
Size: 32 188 32 3476 32 3243
Fragments arranged by size:
3476 3243 188 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7003
XbaI T'CTAG_A
Cuts at: 4339 4339
Size: 7003
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2267 3569 2267
Size: 1302 5701
XhoI C'TCGA_G
Cuts at: 1943 3364 1943
Size: 1421 5582
XmaI C'CCGG_G
Cuts at: 1870 4328 1870
Size: 2458 4545
XmnI GAAnn'nnTTC
Cuts at: 2385 4192 5132 2385
Size: 1807 940 4256
Fragments arranged by size:
4256 1807 940
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4811 276
Size: 53 83 186 936 3277 2468
Fragments arranged by size:
3277 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I EcoICRI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspOMI SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI