Restriction map for
pFN21AB9759
(Circular) (Six-base) MAPSORT of: pFN21AB9759.seq Check: 2396 from: 1 to: 6847
pFN21AB9759 6847 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2427 2427
Size: 6847
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4657 278
Size: 53 83 186 936 3121 2468
Fragments arranged by size:
3121 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4190 1937
Size: 2253 4594
Acc65I G'GTAC_C
Cuts at: 4168 4168
Size: 6847
AclI AA'CG_TT
Cuts at: 1563 4974 5347 1563
Size: 3411 373 3063
Fragments arranged by size:
3411 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3953 4910 6294 6562 1632
Size: 2321 957 1384 268 1917
Fragments arranged by size:
2321 1917 1384 957 268
AfeI AGC'GCT
Cuts at: 3011 3342 3011
Size: 331 6516
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6650
AflIII A'CryG_T
Cuts at: 1203 2604 3258 3645 5746 6723 1203
Size: 1401 654 387 2101 977 1327
Fragments arranged by size:
2101 1401 1327 977 654 387
AgeI A'CCGG_T
Cuts at: 3155 5678 3155
Size: 2523 4324
AhdI GACnn_n'nnGTC
Cuts at: 5576 5576
Size: 6847
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6847
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6847
AlwNI CAG_nnn'CTG
Cuts at: 2047 2836 4590 6162 2047
Size: 789 1754 1572 2732
Fragments arranged by size:
2732 1754 1572 789
ApaI G_GGCC'C
Cuts at: 2567 3057 3108 3399 4113 2567
Size: 490 51 291 714 5301
Fragments arranged by size:
5301 714 490 291 51
ApaLI G'TGCA_C
Cuts at: 4904 6060 4904
Size: 1156 5691
ApoI r'AATT_y
Cuts at: 1493 4156 4382 4534 6643 1493
Size: 2663 226 152 2109 1697
Fragments arranged by size:
2663 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5401 160
Size: 872 4369 1606
Fragments arranged by size:
4369 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6847
AvaI C'yCGr_G
Cuts at: 1870 1943 2305 2560 2887 2894 3047 3194 3233
Size: 73 362 255 327 7 153 147 39
Cuts at: 3233 3703 3884 4172 1870
Size: 470 181 288 4545
Fragments arranged by size:
4545 470 362 327 288 255 181 153
147 73 39 7
AvrII C'CTAG_G
Cuts at: 3391 3391
Size: 6847
BamHI G'GATC_C
Cuts at: 4177 4544 4177
Size: 367 6480
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2023 2327 2707 3070 4168
Size: 325 217 627 236 304 380 363 1098
Cuts at: 4168 5623 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1098 627 380 363 325 304
236 217
BanII G_rGCy'C
Cuts at: 1391 2219 2567 2693 2702 2901 3057 3108 3234
Size: 828 348 126 9 199 156 51 126
Cuts at: 3234 3399 3436 3840 4113 4166 1391
Size: 165 37 404 273 53 4072
Fragments arranged by size:
4072 828 404 348 273 199 165 156
126 126 53 51 37 9
BbeI G_GCGC'C
Cuts at: 2027 2331 2711 3074 2027
Size: 304 380 363 5800
Fragments arranged by size:
5800 380 363 304
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3533 928
Size: 971 1634 4242
Fragments arranged by size:
4242 1634 971
BbvCI CC'TCA_GC
Cuts at: 4055 4055
Size: 6847
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5038 5072 1903
Size: 34 50 34 3017 34 3678
Fragments arranged by size:
3678 3017 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4739 5949 4739
Size: 1210 5637
BclI T'GATC_A
Cuts at: 1548 2094 1548
Size: 546 6301
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2309 2336 2697
Size: 107 122 71 631 605 637 27 361
Cuts at: 2697 2716 3052 3362 3371 3380 3621 3809 3861
Size: 19 336 310 9 9 241 188 52
Cuts at: 3861 5458 136
Size: 1597 1525
Fragments arranged by size:
1597 1525 637 631 605 361 336 310
241 188 122 107 71 52 27 19
9 9
BglII A'GATC_T
Cuts at: 6842 6842
Size: 6847
BlpI GC'TnA_GC
Cuts at: 2313 2727 3926 4082 4264 2313
Size: 414 1199 156 182 4896
Fragments arranged by size:
4896 1199 414 182 156
Bme1580I G_kGCm'C
Cuts at: 1790 2567 3057 3108 3399 4113 4908 6064 1790
Size: 777 490 51 291 714 795 1156 2573
Fragments arranged by size:
2573 1156 795 777 714 490 291 51
BmgBI CAC'GTC
Cuts at: 1318 2092 1318
Size: 774 6073
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2516 4578 5536
Size: 416 53 83 399 24 1099 2062 958
Cuts at: 5536 442
Size: 1753
Fragments arranged by size:
2062 1753 1099 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6847
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2575 3013 2575
Size: 438 6409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2020 2858 4031 5507 1239
Size: 781 838 1173 1476 2579
Fragments arranged by size:
2579 1476 1173 838 781
Bpu10I CC'TnA_GC
Cuts at: 4055 4055
Size: 6847
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2147 2378 3611 4972 5837 6135 2147
Size: 231 1233 1361 865 298 2859
Fragments arranged by size:
2859 1361 1233 865 298 231
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3146 5510 882
Size: 617 1647 2364 2219
Fragments arranged by size:
2364 2219 1647 617
BsaAI yAC'GTr
Cuts at: 493 1204 3259 3313 3325 4638 493
Size: 711 2055 54 12 1313 2702
Fragments arranged by size:
2702 2055 1313 711 54 12
BsaBI GATnn'nnATC
Cuts at: 6841 6841
Size: 6847
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2024 2328 2708 3071
Size: 53 83 186 936 491 304 380 363
Cuts at: 3071 3846 3891 4654 5036 5671 275
Size: 775 45 763 382 635 1451
Fragments arranged by size:
1451 936 775 763 635 491 382 380
363 304 186 83 53 45
BsaWI w'CCGG_w
Cuts at: 3155 5280 5678 5952 6099 6510 3155
Size: 2125 398 274 147 411 3492
Fragments arranged by size:
3492 2125 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6622
Fragments arranged by size:
6622 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2855 2918 3164 3344 3347 1186
Size: 1669 63 246 180 3 4686
Fragments arranged by size:
4686 1669 246 180 63 3
BseYI C'CCAG_C
Cuts at: 2762 2980 3002 3173 3287 3377 4048 6050 6546
Size: 218 22 171 114 90 671 2002 496
Cuts at: 6546 2762
Size: 3063
Fragments arranged by size:
3063 2002 671 496 218 171 114 90
22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2126 3974 1163
Size: 573 390 1848 4036
Fragments arranged by size:
4036 1848 573 390
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2223 2260 2292 2298 2468 2625
Size: 275 186 222 37 32 6 170 157
Cuts at: 2625 2720 2908 3039 3366 3804 3813 3918 3954
Size: 95 188 131 327 438 9 105 36
Cuts at: 3954 3960 4044 4074 4107 4120 4132 4297 5058
Size: 6 84 30 33 13 12 165 761
Cuts at: 5058 6086 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 438 327 275 222 188
186 170 165 157 131 105 95 84
37 36 33 32 30 13 12 9
6 6
BsiHKAI G_wGCw'C
Cuts at: 3690 4908 4993 6064 3690
Size: 1218 85 1071 4473
Fragments arranged by size:
4473 1218 1071 85
BsmI GAATG_Cn'
Cuts at: 3264 4358 4451 3264
Size: 1094 93 5660
Fragments arranged by size:
5660 1094 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3046 3046
Size: 6847
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2219 2567 2693 2702 2901 3057 3108
Size: 399 429 348 126 9 199 156 51
Cuts at: 3108 3234 3399 3436 3690 3840 4113 4166 4908
Size: 126 165 37 254 150 273 53 742
Cuts at: 4908 4993 6064 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 429 399 348 273 254
199 165 156 150 126 126 85 53
51 37 9
BspEI T'CCGG_A
Cuts at: 6510 6510
Size: 6847
BspHI T'CATG_A
Cuts at: 4736 6466 4736
Size: 1730 5117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2196 2361 2427 4202 844
Size: 1352 165 66 1775 3489
Fragments arranged by size:
3489 1775 1352 165 66
BsrBI CCG'CTC
Cuts at: 1394 1823 2716 3061 4734 1394
Size: 429 893 345 1673 3507
Fragments arranged by size:
3507 1673 893 429 345
BsrDI GCAATG_nn'
Cuts at: 65 1248 5342 5516 65
Size: 1183 4094 174 1396
Fragments arranged by size:
4094 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2255 2494 2768 3155 3386 3803 5491
Size: 110 445 239 274 387 231 417 1688
Cuts at: 5491 5678 1700
Size: 187 2869
Fragments arranged by size:
2869 1688 445 417 387 274 239 231
187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6847
BssHII G'CGCG_C
Cuts at: 1925 2124 1925
Size: 199 6648
BssSI C'ACGA_G
Cuts at: 2369 2948 3521 4907 5919 2369
Size: 579 573 1386 1012 3297
Fragments arranged by size:
3297 1386 1012 579 573
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2047 2412 2636 3270 3564 3988 794
Size: 1253 365 224 634 294 424 3653
Fragments arranged by size:
3653 1253 634 424 365 294 224
BstBI TT'CG_AA
Cuts at: 5651 5651
Size: 6847
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6847
BstYI r'GATC_y
Cuts at: 1972 2358 4177 4544 4929 4946 6398 6484 6496
Size: 386 1819 367 385 17 1452 86 12
Cuts at: 6496 6842 1972
Size: 346 1977
Fragments arranged by size:
1977 1819 1452 386 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2292 2298 2401 2547 2574 2703 3789
Size: 552 1227 6 103 146 27 129 1086
Cuts at: 3789 3954 513
Size: 165 3406
Fragments arranged by size:
3406 1227 1086 552 165 146 129 103
27 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6108
Fragments arranged by size:
6108 683 56
BtsI GCAGTG_nn'
Cuts at: 2587 4363 5157 5177 2587
Size: 1776 794 20 4257
Fragments arranged by size:
4257 1776 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6847
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6812
DraI TTT'AAA
Cuts at: 4151 4151
Size: 6847
DraIII CAC_nnn'GTG
Cuts at: 2636 3790 2636
Size: 1154 5693
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3038 5854 809
Size: 507 1722 2816 1802
Fragments arranged by size:
2816 1802 1722 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 2145 2220
Size: 54 1425 50 275 18 199 116 75
Cuts at: 2220 2257 2289 2295 2337 2465 2622 2717 2905
Size: 37 32 6 42 128 157 95 188
Cuts at: 2905 3036 3187 3363 3372 3504 3801 3810 3915
Size: 131 151 176 9 132 297 9 105
Cuts at: 3915 3951 3957 4029 4041 4071 4104 4117 4294
Size: 36 6 72 12 30 33 13 177
Cuts at: 4294 5183 6812 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 297 275 199 188 177
176 157 151 132 131 128 116 105
95 75 72 54 50 43 42 37
36 33 32 30 18 13 12 9
9 6 6
EagI C'GGCC_G
Cuts at: 1537 1812 2220 2257 2289 2295 2465 2622 2717
Size: 275 408 37 32 6 170 157 95
Cuts at: 2717 2905 3036 3363 3801 3810 3915 3951 3957
Size: 188 131 327 438 9 105 36 6
Cuts at: 3957 4041 4071 4104 4117 4294 1537
Size: 84 30 33 13 177 4090
Fragments arranged by size:
4090 438 408 327 275 188 177 170
157 131 105 95 84 37 36 33
32 30 13 9 6 6
EarI CTCTTCn'nnn_
Cuts at: 1353 2123 2681 3012 4777 1353
Size: 770 558 331 1765 3423
Fragments arranged by size:
3423 1765 770 558 331
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2219 2523 5434 5808 5954 387
Size: 1064 138 630 304 2911 374 146 1280
Fragments arranged by size:
2911 1280 1064 630 374 304 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 2858 3953 4031 4910 5507 6294
Size: 393 388 838 1095 78 879 597 787
Cuts at: 6294 6562 1239
Size: 268 1524
Fragments arranged by size:
1524 1095 879 838 787 597 393 388
268 78
EcoNI CCTnn'n_nnAGG
Cuts at: 3405 3405
Size: 6847
EcoO109I rG'GnC_Cy
Cuts at: 2476 2612 2891 3053 3054 3169 3395 3396 3499
Size: 136 279 162 1 115 226 1 103
Cuts at: 3499 2476
Size: 5824
Fragments arranged by size:
5824 279 226 162 136 115 103 1
1
EcoRI G'AATT_C
Cuts at: 4156 4534 4156
Size: 378 6469
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2098 3895 2098
Size: 1797 5050
FspI TGC'GCA
Cuts at: 3611 3695 5353 3611
Size: 84 1658 5105
Fragments arranged by size:
5105 1658 84
FspAI rTGC'GCAy
Cuts at: 3695 3695
Size: 6847
HaeII r_GCGC'y
Cuts at: 1678 2027 2157 2331 2378 2401 2650 2711 2769
Size: 349 130 174 47 23 249 61 58
Cuts at: 2769 3013 3074 3344 3821 4010 4095 5994 1678
Size: 244 61 270 477 189 85 1899 2531
Fragments arranged by size:
2531 1899 477 349 270 249 244 189
174 130 85 61 61 58 47 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4121 4153 5488 5520 5562
Size: 32 135 32 2549 32 1335 32 42
Cuts at: 5562 5594 1373
Size: 32 2626
Fragments arranged by size:
2626 2549 1335 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2581 4191 4437 669
Size: 988 281 643 1610 246 3079
Fragments arranged by size:
3079 1610 988 643 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6847
HpaI GTT'AAC
Cuts at: 4437 4437
Size: 6847
KasI G'GCGC_C
Cuts at: 2023 2327 2707 3070 2023
Size: 304 380 363 5800
Fragments arranged by size:
5800 380 363 304
KpnI G_GTAC'C
Cuts at: 4172 4172
Size: 6847
MfeI C'AATT_G
Cuts at: 4446 4446
Size: 6847
MluI A'CGCG_T
Cuts at: 2604 3645 6723 2604
Size: 1041 3078 2728
Fragments arranged by size:
3078 2728 1041
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2883 5961 6145 1305
Size: 1578 3078 184 2007
Fragments arranged by size:
3078 2007 1578 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3189 4031 10
Size: 54 1425 343 1357 842 2826
Fragments arranged by size:
2826 1425 1357 842 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2102 3779 4805 5164 5323 518
Size: 648 936 1677 1026 359 159 2042
Fragments arranged by size:
2042 1677 1026 936 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2044 2294 2549 2576 2652 2705 2722 2821
Size: 438 250 255 27 76 53 17 99
Cuts at: 2821 2880 3301 3377 3471 3925 3956 4027 4263
Size: 59 421 76 94 454 31 71 236
Cuts at: 4263 4940 6088 6333 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 454 438 421 255 250
245 236 99 94 76 76 71 59
53 31 27 17
NaeI GCC'GGC
Cuts at: 2770 3388 3805 2770
Size: 618 417 5812
Fragments arranged by size:
5812 618 417
NarI GG'CG_CC
Cuts at: 2024 2328 2708 3071 2024
Size: 304 380 363 5800
Fragments arranged by size:
5800 380 363 304
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6295
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6847
NgoMIV G'CCGG_C
Cuts at: 2768 3386 3803 2768
Size: 618 417 5812
Fragments arranged by size:
5812 618 417
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6847
NotI GC'GGCC_GC
Cuts at: 2289 2717 3951 4294 2289
Size: 428 1234 343 4842
Fragments arranged by size:
4842 1234 428 343
NspI r_CATG'y
Cuts at: 1132 1229 3610 3658 3694 4205 5750 1132
Size: 97 2381 48 36 511 1545 2229
Fragments arranged by size:
2381 2229 1545 511 97 48 36
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6847
PciI A'CATG_T
Cuts at: 5746 5746
Size: 6847
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2209 2836 1108
Size: 1101 627 5119
Fragments arranged by size:
5119 1101 627
PmeI GTTT'AAAC
Cuts at: 4151 4151
Size: 6847
PmlI CAC'GTG
Cuts at: 3313 3325 3313
Size: 12 6835
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4916 4948 6429 6461 4916
Size: 32 1481 32 5302
Fragments arranged by size:
5302 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2612 2891 3169 3499 2612
Size: 279 278 330 5960
Fragments arranged by size:
5960 330 279 278
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6847
PsiI TTA'TAA
Cuts at: 4417 4417
Size: 6847
PspOMI G'GGCC_C
Cuts at: 2563 3053 3104 3395 4109 2563
Size: 490 51 291 714 5301
Fragments arranged by size:
5301 714 490 291 51
PspXI vC'TCGA_Gb
Cuts at: 3194 3194
Size: 6847
PstI C_TGCA'G
Cuts at: 830 2055 2358 4199 830
Size: 1225 303 1841 3478
Fragments arranged by size:
3478 1841 1225 303
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6847
PvuII CAG'CTG
Cuts at: 2044 3301 3925 2044
Size: 1257 624 4966
Fragments arranged by size:
4966 1257 624
SacII CC_GC'GG
Cuts at: 2295 2550 2577 2706 3957 2295
Size: 255 27 129 1251 5185
Fragments arranged by size:
5185 1251 255 129 27
SalI G'TCGA_C
Cuts at: 1936 4189 1936
Size: 2253 4594
SanDI GG'GwC_CC
Cuts at: 2612 2891 2612
Size: 279 6568
SapI GCTCTTCn'nnn_
Cuts at: 2681 3012 2681
Size: 331 6516
SbfI CC_TGCA'GG
Cuts at: 4199 4199
Size: 6847
ScaI AGT'ACT
Cuts at: 2411 5095 6807 2411
Size: 2684 1712 2451
Fragments arranged by size:
2684 2451 1712
SfcI C'TryA_G
Cuts at: 826 1045 2051 2354 4195 5330 6011 6202 826
Size: 219 1006 303 1841 1135 681 191 1471
Fragments arranged by size:
1841 1471 1135 1006 681 303 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3362 3371 3380 3809 3362
Size: 9 9 429 6400
Fragments arranged by size:
6400 429 9 9
SfoI GGC'GCC
Cuts at: 2025 2329 2709 3072 2025
Size: 304 380 363 5800
Fragments arranged by size:
5800 380 363 304
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6847
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6847
SmaI CCC'GGG
Cuts at: 1872 2307 2889 3049 4174 1872
Size: 435 582 160 1125 4545
Fragments arranged by size:
4545 1125 582 435 160
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2162 2393 3194 3626 4951 5852
Size: 197 926 219 231 801 432 1325 901
Cuts at: 5852 6114 820
Size: 262 1553
Fragments arranged by size:
1553 1325 926 901 801 432 262 231
219 197
SnaBI TAC'GTA
Cuts at: 493 4638 493
Size: 4145 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6847
SphI G_CATG'C
Cuts at: 1132 3658 4205 1132
Size: 2526 547 3774
Fragments arranged by size:
3774 2526 547
SspI AAT'ATT
Cuts at: 5 52 4771 5
Size: 47 4719 2081
Fragments arranged by size:
4719 2081 47
StuI AGG'CCT
Cuts at: 1517 2507 1517
Size: 990 5857
StyI C'CwwG_G
Cuts at: 513 1065 2073 3391 3999 4286 513
Size: 552 1008 1318 608 287 3074
Fragments arranged by size:
3074 1318 1008 608 552 287
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4874 4891 5044 5229 5674 325
Size: 1255 3294 17 153 185 445 1498
Fragments arranged by size:
3294 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2409 5093 6805
Size: 274 80 33 51 1443 432 2684 1712
Cuts at: 6805 96
Size: 138
Fragments arranged by size:
2684 1712 1443 432 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6807 6839 6807
Size: 32 6815
Tth111I GACn'n_nGTC
Cuts at: 1316 3422 1316
Size: 2106 4741
XbaI T'CTAG_A
Cuts at: 4183 4183
Size: 6847
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4006 4006
Size: 6847
XhoI C'TCGA_G
Cuts at: 1943 3194 1943
Size: 1251 5596
XmaI C'CCGG_G
Cuts at: 1870 2305 2887 3047 4172 1870
Size: 435 582 160 1125 4545
Fragments arranged by size:
4545 1125 582 435 160
XmnI GAAnn'nnTTC
Cuts at: 3575 4976 3575
Size: 1401 5446
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4655 276
Size: 53 83 186 936 3121 2468
Fragments arranged by size:
3121 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I
EcoICRI EcoRV FseI NruI NsiI PacI PfoI PsrI
RsrII SacI SexAI SrfI SwaI