Restriction map for
pFN21AB9756
(Circular) (Six-base) MAPSORT of: pFN21AB9756.seq Check: 4689 from: 1 to: 6820
pFN21AB9756 6820 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4630 278
Size: 53 83 186 936 3094 2468
Fragments arranged by size:
3094 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2811 3966 4163 1937
Size: 874 1155 197 4594
Fragments arranged by size:
4594 1155 874 197
Acc65I G'GTAC_C
Cuts at: 4141 4141
Size: 6820
AclI AA'CG_TT
Cuts at: 1563 4947 5320 1563
Size: 3384 373 3063
Fragments arranged by size:
3384 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3349 3381 3710 3885 4077 4883 6267 6535
Size: 1717 32 329 175 192 806 1384 268
Cuts at: 6535 1632
Size: 1917
Fragments arranged by size:
1917 1717 1384 806 329 268 192 175
32
AfeI AGC'GCT
Cuts at: 2101 2278 2101
Size: 177 6643
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6623
AflIII A'CryG_T
Cuts at: 1203 5719 6696 1203
Size: 4516 977 1327
Fragments arranged by size:
4516 1327 977
AgeI A'CCGG_T
Cuts at: 5651 5651
Size: 6820
AhdI GACnn_n'nnGTC
Cuts at: 2155 5549 2155
Size: 3394 3426
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6820
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3309 1184
Size: 2125 4695
AlwNI CAG_nnn'CTG
Cuts at: 2792 3302 4563 6135 2792
Size: 510 1261 1572 3477
Fragments arranged by size:
3477 1572 1261 510
ApaI G_GGCC'C
Cuts at: 2230 2304 2230
Size: 74 6746
ApaLI G'TGCA_C
Cuts at: 4877 6033 4877
Size: 1156 5664
ApoI r'AATT_y
Cuts at: 1493 2840 4021 4129 4355 4507 6616 1493
Size: 1347 1181 108 226 152 2109 1697
Fragments arranged by size:
2109 1697 1347 1181 226 152 108
AseI AT'TA_AT
Cuts at: 160 1032 4050 5374 160
Size: 872 3018 1324 1606
Fragments arranged by size:
3018 1606 1324 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6820
AvaI C'yCGr_G
Cuts at: 1870 1943 2612 2686 4145 1870
Size: 73 669 74 1459 4545
Fragments arranged by size:
4545 1459 669 74 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2516 2549 2516
Size: 33 6787
BamHI G'GATC_C
Cuts at: 4150 4517 4150
Size: 367 6453
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2515 2690 4141 5596 618
Size: 325 217 627 728 175 1451 1455 1842
Fragments arranged by size:
1842 1455 1451 728 627 325 217 175
BanII G_rGCy'C
Cuts at: 1391 2036 2208 2230 2304 2324 2426 2716 3252
Size: 645 172 22 74 20 102 290 536
Cuts at: 3252 3498 4139 1391
Size: 246 641 4072
Fragments arranged by size:
4072 645 641 536 290 246 172 102
74 22 20
BbeI G_GCGC'C
Cuts at: 2519 2519
Size: 6820
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5849
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5011 5045 1903
Size: 34 50 34 2990 34 3678
Fragments arranged by size:
3678 2990 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4712 5922 4712
Size: 1210 5610
BclI T'GATC_A
Cuts at: 1548 2865 3340 1548
Size: 1317 475 5028
Fragments arranged by size:
5028 1317 475
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2234 2319 2384
Size: 107 122 71 631 605 562 85 65
Cuts at: 2384 2747 2833 5431 136
Size: 363 86 2598 1525
Fragments arranged by size:
2598 1525 631 605 562 363 122 107
86 85 71 65
BglII A'GATC_T
Cuts at: 6815 6815
Size: 6820
BlpI GC'TnA_GC
Cuts at: 4237 4237
Size: 6820
Bme1580I G_kGCm'C
Cuts at: 1790 2230 2304 2609 2693 2806 4881 6037 1790
Size: 440 74 305 84 113 2075 1156 2573
Fragments arranged by size:
2573 2075 1156 440 305 113 84 74
BmgBI CAC'GTC
Cuts at: 1318 2809 1318
Size: 1491 5329
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2777 4551 5509
Size: 416 53 83 399 24 1360 1774 958
Cuts at: 5509 442
Size: 1753
Fragments arranged by size:
1774 1753 1360 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6820
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 6820
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2426 2757 3480 3823 3994 5480 1239
Size: 851 336 331 723 343 171 1486 2579
Fragments arranged by size:
2579 1486 851 723 343 336 331 171
Bpu10I CC'TnA_GC
Cuts at: 2420 2420
Size: 6820
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2923 3501 4945 5810 6108 2923
Size: 578 1444 865 298 3635
Fragments arranged by size:
3635 1444 865 578 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5483 882
Size: 617 3984 2219
Fragments arranged by size:
3984 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4611 493
Size: 711 3407 2702
Fragments arranged by size:
3407 2702 711
BsaBI GATnn'nnATC
Cuts at: 6814 6814
Size: 6820
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2256 2516 4627 5009
Size: 53 83 186 936 723 260 2111 382
Cuts at: 5009 5644 275
Size: 635 1451
Fragments arranged by size:
2111 1451 936 723 635 382 260 186
83 53
BsaWI w'CCGG_w
Cuts at: 5253 5651 5925 6072 6483 5253
Size: 398 274 147 411 5590
Fragments arranged by size:
5590 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3406 3418 3436 3448 1178
Size: 30 165 30 2003 12 18 12 4550
Fragments arranged by size:
4550 2003 165 30 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2121 2351 2354 1186
Size: 935 230 3 5652
Fragments arranged by size:
5652 935 230 3
BseYI C'CCAG_C
Cuts at: 2114 2510 3606 6023 6519 2114
Size: 396 1096 2417 496 2415
Fragments arranged by size:
2417 2415 1096 496 396
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6247
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2314 4270 5031 6059 1540
Size: 275 186 313 1956 761 1028 2301
Fragments arranged by size:
2301 1956 1028 761 313 275 186
BsiHKAI G_wGCw'C
Cuts at: 2036 2426 4881 4966 6037 2036
Size: 390 2455 85 1071 2819
Fragments arranged by size:
2819 2455 1071 390 85
BsmI GAATG_Cn'
Cuts at: 4331 4424 4331
Size: 93 6727
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2208 2230 2304 2324 2426 2609
Size: 399 246 172 22 74 20 102 183
Cuts at: 2609 2693 2716 2806 3252 3498 4139 4881 4966
Size: 84 23 90 446 246 641 742 85
Cuts at: 4966 6037 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 641 446 399 246 246
183 172 102 90 85 84 74 23
22 20
BspEI T'CCGG_A
Cuts at: 6483 6483
Size: 6820
BspHI T'CATG_A
Cuts at: 4709 6439 4709
Size: 1730 5090
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2052 4175 844
Size: 1208 2123 3489
Fragments arranged by size:
3489 2123 1208
BsrBI CCG'CTC
Cuts at: 1394 1823 2199 2431 2719 4707 1394
Size: 429 376 232 288 1988 3507
Fragments arranged by size:
3507 1988 429 376 288 232
BsrDI GCAATG_nn'
Cuts at: 65 1248 3291 3692 3780 5315 5489 65
Size: 1183 2043 401 88 1535 174 1396
Fragments arranged by size:
2043 1535 1396 1183 401 174 88
BsrFI r'CCGG_y
Cuts at: 1700 1810 2639 2823 5464 5651 1700
Size: 110 829 184 2641 187 2869
Fragments arranged by size:
2869 2641 829 187 184 110
BsrGI T'GTAC_A
Cuts at: 96 3387 96
Size: 3291 3529
BssHII G'CGCG_C
Cuts at: 1925 2052 2399 2552 1925
Size: 127 347 153 6193
Fragments arranged by size:
6193 347 153 127
BssSI C'ACGA_G
Cuts at: 4880 5892 4880
Size: 1012 5808
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3302 794
Size: 2508 4312
BstBI TT'CG_AA
Cuts at: 5624 5624
Size: 6820
BstEII G'GTnAC_C
Cuts at: 1184 3007 1184
Size: 1823 4997
BstXI CCAn_nnnn'nTGG
Cuts at: 3638 3638
Size: 6820
BstYI r'GATC_y
Cuts at: 1972 2753 3440 4150 4517 4902 4919 6371 6457
Size: 781 687 710 367 385 17 1452 86
Cuts at: 6457 6469 6815 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 781 710 687 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3967 3967
Size: 6820
Bsu36I CC'TnA_GG
Cuts at: 2457 2457
Size: 6820
BtgI C'CryG_G
Cuts at: 513 1065 2232 2382 2409 2521 2595 3426 3830
Size: 552 1167 150 27 112 74 831 404
Cuts at: 3830 513
Size: 3503
Fragments arranged by size:
3503 1167 831 552 404 150 112 74
27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6081
Fragments arranged by size:
6081 683 56
BtsI GCAGTG_nn'
Cuts at: 2292 2394 2656 2931 3303 4336 5130 5150 2292
Size: 102 262 275 372 1033 794 20 3962
Fragments arranged by size:
3962 1033 794 372 275 262 102 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6820
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6785
DraI TTT'AAA
Cuts at: 4124 4124
Size: 6820
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5827 809
Size: 507 4511 1802
Fragments arranged by size:
4511 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2104 2235 2289
Size: 54 1425 50 275 18 274 131 54
Cuts at: 2289 2311 2391 3594 4267 5156 6785 8
Size: 22 80 1203 673 889 1629 43
Fragments arranged by size:
1629 1425 1203 889 673 275 274 131
80 54 54 50 43 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 2311 4267 1537
Size: 275 499 1956 4090
Fragments arranged by size:
4090 1956 499 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2495 3582 3913 4053 4750 1353
Size: 1142 1087 331 140 697 3423
Fragments arranged by size:
3423 1142 1087 697 331 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2341 2984 5407 5781 5927 387
Size: 1064 138 752 643 2423 374 146 1280
Fragments arranged by size:
2423 1280 1064 752 643 374 146 138
EcoICRI GAG'CTC
Cuts at: 2034 2424 2034
Size: 390 6430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2426 2757 3349 3381 3480 3710
Size: 393 458 336 331 592 32 99 230
Cuts at: 3710 3823 3885 3994 4077 4883 5480 6267 6535
Size: 113 62 109 83 806 597 787 268
Cuts at: 6535 1239
Size: 1524
Fragments arranged by size:
1524 806 787 597 592 458 393 336
331 268 230 113 109 99 83 62
32
EcoO109I rG'GnC_Cy
Cuts at: 2226 2227 2300 2301 2773 2226
Size: 1 73 1 472 6273
Fragments arranged by size:
6273 472 73 1 1
EcoRI G'AATT_C
Cuts at: 4129 4507 4129
Size: 378 6442
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2161 2161
Size: 6820
FspI TGC'GCA
Cuts at: 5326 5326
Size: 6820
HaeII r_GCGC'y
Cuts at: 1678 2103 2280 2519 2989 5967 1678
Size: 425 177 239 470 2978 2531
Fragments arranged by size:
2978 2531 470 425 239 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2141 2173 3860 3892 5461
Size: 32 135 32 569 32 1687 32 1569
Cuts at: 5461 5493 5535 5567 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1687 1569 569 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3414 3505 4164 4410 669
Size: 988 281 1476 91 659 246 3079
Fragments arranged by size:
3079 1476 988 659 281 246 91
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6820
HpaI GTT'AAC
Cuts at: 3414 4410 3414
Size: 996 5824
KasI G'GCGC_C
Cuts at: 2515 2515
Size: 6820
KpnI G_GTAC'C
Cuts at: 4145 4145
Size: 6820
MfeI C'AATT_G
Cuts at: 4419 4419
Size: 6820
MluI A'CGCG_T
Cuts at: 6696 6696
Size: 6820
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2121 2445 5934 6118 1305
Size: 816 324 3489 184 2007
Fragments arranged by size:
3489 2007 816 324 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2106 2291 3596 10
Size: 54 1425 343 274 185 1305 3234
Fragments arranged by size:
3234 1425 1305 343 274 185 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2943 3161 3296 4778 5137 5296 518
Size: 648 1777 218 135 1482 359 159 2042
Fragments arranged by size:
2042 1777 1482 648 359 218 159 135
MspA1I CmG'CkG
Cuts at: 1606 2068 2091 2234 2357 2384 2597 2670 2747
Size: 462 23 143 123 27 213 73 77
Cuts at: 2747 3610 3821 4236 4913 6061 6306 1606
Size: 863 211 415 677 1148 245 2120
Fragments arranged by size:
2120 1148 863 677 462 415 245 213
211 143 123 77 73 27 23
NarI GG'CG_CC
Cuts at: 2516 2516
Size: 6820
NcoI C'CATG_G
Cuts at: 513 1065 2409 3830 513
Size: 552 1344 1421 3503
Fragments arranged by size:
3503 1421 1344 552
NdeI CA'TA_TG
Cuts at: 387 3703 387
Size: 3316 3504
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6820
NotI GC'GGCC_GC
Cuts at: 4267 4267
Size: 6820
NspI r_CATG'y
Cuts at: 1132 1229 2893 3037 4178 5723 1132
Size: 97 1664 144 1141 1545 2229
Fragments arranged by size:
2229 1664 1545 1141 144 97
PasI CC'CwG_GG
Cuts at: 1348 3742 1348
Size: 2394 4426
PciI A'CATG_T
Cuts at: 5719 5719
Size: 6820
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2792 2982 3742 1108
Size: 1684 190 760 4186
Fragments arranged by size:
4186 1684 760 190
PfoI T'CCnGG_A
Cuts at: 3489 3489
Size: 6820
PmeI GTTT'AAAC
Cuts at: 4124 4124
Size: 6820
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4889 4921 6402 6434 4889
Size: 32 1481 32 5275
Fragments arranged by size:
5275 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6820
PsiI TTA'TAA
Cuts at: 3531 4390 3531
Size: 859 5961
PspOMI G'GGCC_C
Cuts at: 2226 2300 2226
Size: 74 6746
PstI C_TGCA'G
Cuts at: 830 2362 4172 830
Size: 1532 1810 3478
Fragments arranged by size:
3478 1810 1532
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6820
PvuII CAG'CTG
Cuts at: 3821 3821
Size: 6820
SacI G_AGCT'C
Cuts at: 2036 2426 2036
Size: 390 6430
SacII CC_GC'GG
Cuts at: 2235 2385 2598 2235
Size: 150 213 6457
Fragments arranged by size:
6457 213 150
SalI G'TCGA_C
Cuts at: 1936 4162 1936
Size: 2226 4594
SapI GCTCTTCn'nnn_
Cuts at: 3913 3913
Size: 6820
SbfI CC_TGCA'GG
Cuts at: 4172 4172
Size: 6820
ScaI AGT'ACT
Cuts at: 5068 6780 5068
Size: 1712 5108
SfcI C'TryA_G
Cuts at: 826 1045 2358 4168 5303 5984 6175 826
Size: 219 1313 1810 1135 681 191 1471
Fragments arranged by size:
1810 1471 1313 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2234 2234
Size: 6820
SfoI GGC'GCC
Cuts at: 2517 2517
Size: 6820
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6820
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6820
SmaI CCC'GGG
Cuts at: 1872 2614 2688 4147 1872
Size: 742 74 1459 4545
Fragments arranged by size:
4545 1459 742 74
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2902 3516 4924 5825 6087 820
Size: 197 926 959 614 1408 901 262 1553
Fragments arranged by size:
1553 1408 959 926 901 614 262 197
SnaBI TAC'GTA
Cuts at: 493 4611 493
Size: 4118 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6820
SphI G_CATG'C
Cuts at: 1132 4178 1132
Size: 3046 3774
SrfI GCCC'GGGC
Cuts at: 2688 2688
Size: 6820
SspI AAT'ATT
Cuts at: 5 52 4744 5
Size: 47 4692 2081
Fragments arranged by size:
4692 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6820
StyI C'CwwG_G
Cuts at: 513 1065 2409 3830 3879 4259 513
Size: 552 1344 1421 49 380 3074
Fragments arranged by size:
3074 1421 1344 552 380 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2995 3075 3173 4847 4864 5017 5202
Size: 1255 1415 80 98 1674 17 153 185
Cuts at: 5202 5647 325
Size: 445 1498
Fragments arranged by size:
1674 1498 1415 1255 445 185 153 98
80 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2700 3387 5066
Size: 274 80 33 51 1443 723 687 1679
Cuts at: 5066 6778 96
Size: 1712 138
Fragments arranged by size:
1712 1679 1443 723 687 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3418 3450 6780 6812 3418
Size: 32 3330 32 3426
Fragments arranged by size:
3426 3330 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6820
XbaI T'CTAG_A
Cuts at: 3943 4156 3943
Size: 213 6607
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2507 2507
Size: 6820
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6820
XmaI C'CCGG_G
Cuts at: 1870 2612 2686 4145 1870
Size: 742 74 1459 4545
Fragments arranged by size:
4545 1459 742 74
XmnI GAAnn'nnTTC
Cuts at: 4949 4949
Size: 6820
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4628 276
Size: 53 83 186 936 3094 2468
Fragments arranged by size:
3094 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbvCI BfrBI BsiWI BsmBI
DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI
SexAI SwaI