Restriction map for
pFN21AB9755
(Circular) (Six-base) MAPSORT of: pFN21AB9755.seq Check: 8610 from: 1 to: 6688
pFN21AB9755 6688 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2181 2181
Size: 6688
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4498 278
Size: 53 83 186 936 2962 2468
Fragments arranged by size:
2962 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4031 1937
Size: 2094 4594
Acc65I G'GTAC_C
Cuts at: 4009 4009
Size: 6688
AclI AA'CG_TT
Cuts at: 1563 4815 5188 1563
Size: 3252 373 3063
Fragments arranged by size:
3252 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2077 4751 6135 6403 1632
Size: 445 2674 1384 268 1917
Fragments arranged by size:
2674 1917 1384 445 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6491
AflIII A'CryG_T
Cuts at: 1203 2828 5587 6564 1203
Size: 1625 2759 977 1327
Fragments arranged by size:
2759 1625 1327 977
AgeI A'CCGG_T
Cuts at: 5519 5519
Size: 6688
AhdI GACnn_n'nnGTC
Cuts at: 5417 5417
Size: 6688
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6688
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6688
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2514 2546 2514
Size: 32 6656
AlwNI CAG_nnn'CTG
Cuts at: 2052 2116 3426 3930 4431 6003 2052
Size: 64 1310 504 501 1572 2737
Fragments arranged by size:
2737 1572 1310 504 501 64
ApaLI G'TGCA_C
Cuts at: 4745 5901 4745
Size: 1156 5532
ApoI r'AATT_y
Cuts at: 1493 2288 2316 2525 2801 2837 2886 3028 3222
Size: 795 28 209 276 36 49 142 194
Cuts at: 3222 3306 3390 3474 3622 3726 3810 3874 3997
Size: 84 84 84 148 104 84 64 123
Cuts at: 3997 4223 4375 6484 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 795 276 226 209 194 152
148 142 123 104 84 84 84 84
64 49 36 28
AseI AT'TA_AT
Cuts at: 160 1032 3038 5242 160
Size: 872 2006 2204 1606
Fragments arranged by size:
2204 2006 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6688
AvaI C'yCGr_G
Cuts at: 1870 1943 4013 1870
Size: 73 2070 4545
Fragments arranged by size:
4545 2070 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2123 2156 2123
Size: 33 6655
BamHI G'GATC_C
Cuts at: 4018 4385 4018
Size: 367 6321
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4009 5464 618
Size: 325 217 627 2222 1455 1842
Fragments arranged by size:
2222 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4007 1391
Size: 2616 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2364 928
Size: 971 465 5252
Fragments arranged by size:
5252 971 465
BbvCI CC'TCA_GC
Cuts at: 2768 2768
Size: 6688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4879 4913 1903
Size: 34 50 34 2858 34 3678
Fragments arranged by size:
3678 2858 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4580 5790 4580
Size: 1210 5478
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6688
BfrBI ATG'CAT
Cuts at: 3046 3298 3466 3970 3046
Size: 252 168 504 5764
Fragments arranged by size:
5764 504 252 168
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5299 136
Size: 107 122 71 631 605 3627 1525
Fragments arranged by size:
3627 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2847 6683 2847
Size: 3836 2852
BlpI GC'TnA_GC
Cuts at: 2915 4105 2915
Size: 1190 5498
Bme1580I G_kGCm'C
Cuts at: 1790 4749 5905 1790
Size: 2959 1156 2573
Fragments arranged by size:
2959 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6688
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4419 5377 442
Size: 416 53 83 399 24 3002 958 1753
Fragments arranged by size:
3002 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6688
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3173 3173
Size: 6688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3168 3252 3420 3443 3588 3672 3924 5348
Size: 1929 84 168 23 145 84 252 1424
Cuts at: 5348 1239
Size: 2579
Fragments arranged by size:
2579 1929 1424 252 168 145 84 84
23
Bpu10I CC'TnA_GC
Cuts at: 2768 2768
Size: 6688
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4813 5678 5976 4813
Size: 865 298 5525
Fragments arranged by size:
5525 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5351 882
Size: 617 3852 2219
Fragments arranged by size:
3852 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4479 493
Size: 711 3275 2702
Fragments arranged by size:
3275 2702 711
BsaBI GATnn'nnATC
Cuts at: 6682 6682
Size: 6688
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2134 4495 4877 5512
Size: 53 83 186 936 601 2361 382 635
Cuts at: 5512 275
Size: 1451
Fragments arranged by size:
2361 1451 936 635 601 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5121 5519 5793 5940 6351 5121
Size: 398 274 147 411 5458
Fragments arranged by size:
5458 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6463
Fragments arranged by size:
6463 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2326 2868 1186
Size: 931 209 542 5006
Fragments arranged by size:
5006 931 542 209
BseYI C'CCAG_C
Cuts at: 2028 5891 6387 2028
Size: 3863 496 2329
Fragments arranged by size:
3863 2329 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3861 1163
Size: 573 2125 3990
Fragments arranged by size:
3990 2125 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4138 4899 5927 1540
Size: 275 186 2137 761 1028 2301
Fragments arranged by size:
2301 2137 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4749 4834 5905 4749
Size: 85 1071 5532
Fragments arranged by size:
5532 1071 85
BsmI GAATG_Cn'
Cuts at: 3170 3422 4199 4292 3170
Size: 252 777 93 5566
Fragments arranged by size:
5566 777 252 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4007 4749 4834 5905 1391
Size: 399 2217 742 85 1071 2174
Fragments arranged by size:
2217 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6351 6351
Size: 6688
BspHI T'CATG_A
Cuts at: 2908 4577 6307 2908
Size: 1669 1730 3289
Fragments arranged by size:
3289 1730 1669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2181 4043 844
Size: 1337 1862 3489
Fragments arranged by size:
3489 1862 1337
BsrBI CCG'CTC
Cuts at: 1394 1823 4575 1394
Size: 429 2752 3507
Fragments arranged by size:
3507 2752 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2644 3091 5183 5357 65
Size: 1183 1396 447 2092 174 1396
Fragments arranged by size:
2092 1396 1396 1183 447 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5332 5519 1700
Size: 110 3522 187 2869
Fragments arranged by size:
3522 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6688
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6688
BssSI C'ACGA_G
Cuts at: 4748 5760 4748
Size: 1012 5676
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6688
BstBI TT'CG_AA
Cuts at: 2190 5492 2190
Size: 3302 3386
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6688
BstYI r'GATC_y
Cuts at: 1972 2847 4018 4385 4770 4787 6239 6325 6337
Size: 875 1171 367 385 17 1452 86 12
Cuts at: 6337 6683 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1171 875 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2240 513
Size: 552 1175 4961
Fragments arranged by size:
4961 1175 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3956 487
Size: 683 56 2730 3219
Fragments arranged by size:
3219 2730 683 56
BtsI GCAGTG_nn'
Cuts at: 2053 3175 3259 3427 4204 4998 5018 2053
Size: 1122 84 168 777 794 20 3723
Fragments arranged by size:
3723 1122 794 777 168 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6688
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3073 3108 529
Size: 35 2509 35 4109
Fragments arranged by size:
4109 2509 35 35
DraI TTT'AAA
Cuts at: 3992 3992
Size: 6688
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5695 809
Size: 507 4379 1802
Fragments arranged by size:
4379 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4135 5024 6653
Size: 54 1425 50 275 18 2305 889 1629
Cuts at: 6653 8
Size: 43
Fragments arranged by size:
2305 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4135 1537
Size: 275 2323 4090
Fragments arranged by size:
4090 2323 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2588 2701 4618 1353
Size: 1235 113 1917 3423
Fragments arranged by size:
3423 1917 1235 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5275 5649 5795 387
Size: 1064 138 3686 374 146 1280
Fragments arranged by size:
3686 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2077 3168 3252 3420 3443 3588 3672
Size: 393 445 1091 84 168 23 145 84
Cuts at: 3672 3924 4751 5348 6135 6403 1239
Size: 252 827 597 787 268 1524
Fragments arranged by size:
1524 1091 827 787 597 445 393 268
252 168 145 84 84 23
EcoNI CCTnn'n_nnAGG
Cuts at: 2141 2141
Size: 6688
EcoO109I rG'GnC_Cy
Cuts at: 3324 3687 3324
Size: 363 6325
EcoRI G'AATT_C
Cuts at: 3028 3306 3390 3474 3726 3810 3997 4375 3028
Size: 278 84 84 252 84 187 378 5341
Fragments arranged by size:
5341 378 278 252 187 84 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3388 3388
Size: 6688
FspI TGC'GCA
Cuts at: 5194 5194
Size: 6688
HaeII r_GCGC'y
Cuts at: 1678 5835 1678
Size: 4157 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3367 3399 3703 3735 5329
Size: 32 135 32 1795 32 304 32 1594
Cuts at: 5329 5361 5403 5435 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1795 1594 304 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4032 4278 669
Size: 988 281 2094 246 3079
Fragments arranged by size:
3079 2094 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6688
HpaI GTT'AAC
Cuts at: 4278 4278
Size: 6688
KpnI G_GTAC'C
Cuts at: 4013 4013
Size: 6688
MfeI C'AATT_G
Cuts at: 4287 4287
Size: 6688
MluI A'CGCG_T
Cuts at: 6564 6564
Size: 6688
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2136 2202 5802 5986 1305
Size: 831 66 3600 184 2007
Fragments arranged by size:
3600 2007 831 184 66
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4866
Fragments arranged by size:
4866 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2046 2577 2979 3835 4646 5005 5164
Size: 648 880 531 402 856 811 359 159
Cuts at: 5164 518
Size: 2042
Fragments arranged by size:
2042 880 856 811 648 531 402 359
159
MspA1I CmG'CkG
Cuts at: 1606 4104 4781 5929 6174 1606
Size: 2498 677 1148 245 2120
Fragments arranged by size:
2498 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2240 513
Size: 552 1175 4961
Fragments arranged by size:
4961 1175 552
NdeI CA'TA_TG
Cuts at: 387 2996 387
Size: 2609 4079
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6688
NotI GC'GGCC_GC
Cuts at: 4135 4135
Size: 6688
NsiI A_TGCA'T
Cuts at: 3048 3300 3468 3972 3048
Size: 252 168 504 5764
Fragments arranged by size:
5764 504 252 168
NspI r_CATG'y
Cuts at: 1132 1229 2832 4046 5591 1132
Size: 97 1603 1214 1545 2229
Fragments arranged by size:
2229 1603 1545 1214 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6688
PciI A'CATG_T
Cuts at: 2828 5587 2828
Size: 2759 3929
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6688
PmeI GTTT'AAAC
Cuts at: 3992 3992
Size: 6688
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4757 4789 6270 6302 4757
Size: 32 1481 32 5143
Fragments arranged by size:
5143 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3324 3324
Size: 6688
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6688
PsiI TTA'TAA
Cuts at: 3750 4258 3750
Size: 508 6180
PstI C_TGCA'G
Cuts at: 830 4040 830
Size: 3210 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6688
SalI G'TCGA_C
Cuts at: 1936 4030 1936
Size: 2094 4594
SbfI CC_TGCA'GG
Cuts at: 4040 4040
Size: 6688
ScaI AGT'ACT
Cuts at: 4936 6648 4936
Size: 1712 4976
SfcI C'TryA_G
Cuts at: 826 1045 2164 3735 4036 5171 5852 6043 826
Size: 219 1119 1571 301 1135 681 191 1471
Fragments arranged by size:
1571 1471 1135 1119 681 301 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6688
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6688
SmaI CCC'GGG
Cuts at: 1872 4015 1872
Size: 2143 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4792 5693 5955 820
Size: 197 926 2849 901 262 1553
Fragments arranged by size:
2849 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4479 493
Size: 3986 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6688
SphI G_CATG'C
Cuts at: 1132 4046 1132
Size: 2914 3774
SspI AAT'ATT
Cuts at: 5 52 2484 3979 4612 5
Size: 47 2432 1495 633 2081
Fragments arranged by size:
2432 2081 1495 633 47
StuI AGG'CCT
Cuts at: 1517 3017 3353 3605 1517
Size: 1500 336 252 4600
Fragments arranged by size:
4600 1500 336 252
StyI C'CwwG_G
Cuts at: 513 1065 2240 4127 513
Size: 552 1175 1887 3074
Fragments arranged by size:
3074 1887 1175 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2870 3962 4715 4732 4885 5070 5515
Size: 1255 1290 1092 753 17 153 185 445
Cuts at: 5515 325
Size: 1498
Fragments arranged by size:
1498 1290 1255 1092 753 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2831 2999 3335
Size: 274 80 33 51 1443 854 168 336
Cuts at: 3335 3799 4934 6646 96
Size: 464 1135 1712 138
Fragments arranged by size:
1712 1443 1135 854 464 336 274 168
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6648 6680 6648
Size: 32 6656
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6688
XbaI T'CTAG_A
Cuts at: 4024 4024
Size: 6688
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6688
XmaI C'CCGG_G
Cuts at: 1870 4013 1870
Size: 2143 4545
XmnI GAAnn'nnTTC
Cuts at: 2849 3017 3437 3521 3605 3773 3857 4817 2849
Size: 168 420 84 84 168 84 960 4720
Fragments arranged by size:
4720 960 420 168 168 84 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4496 276
Size: 53 83 186 936 2962 2468
Fragments arranged by size:
2962 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BsiWI BsmBI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI