Restriction map for
pFN21AB9751
(Circular) (Six-base) MAPSORT of: pFN21AB9751.seq Check: 4998 from: 1 to: 6523
pFN21AB9751 6523 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4333 278
Size: 53 83 186 936 2797 2468
Fragments arranged by size:
2797 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3866 1937
Size: 1929 4594
Acc65I G'GTAC_C
Cuts at: 2905 3844 2905
Size: 939 5584
AclI AA'CG_TT
Cuts at: 1563 4650 5023 1563
Size: 3087 373 3063
Fragments arranged by size:
3087 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2923 3424 4586 5970 6238 1632
Size: 1291 501 1162 1384 268 1917
Fragments arranged by size:
1917 1384 1291 1162 501 268
AfeI AGC'GCT
Cuts at: 3281 3281
Size: 6523
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6326
AflIII A'CryG_T
Cuts at: 1203 5422 6399 1203
Size: 4219 977 1327
Fragments arranged by size:
4219 1327 977
AgeI A'CCGG_T
Cuts at: 5354 5354
Size: 6523
AhdI GACnn_n'nnGTC
Cuts at: 5252 5252
Size: 6523
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6523
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6523
AlwNI CAG_nnn'CTG
Cuts at: 4266 5838 4266
Size: 1572 4951
ApaLI G'TGCA_C
Cuts at: 4580 5736 4580
Size: 1156 5367
ApoI r'AATT_y
Cuts at: 1493 2057 2168 2191 2407 2682 2990 3028 3210
Size: 564 111 23 216 275 308 38 182
Cuts at: 3210 3294 3714 3832 4058 4210 6319 1493
Size: 84 420 118 226 152 2109 1697
Fragments arranged by size:
2109 1697 564 420 308 275 226 216
182 152 118 111 84 38 23
AseI AT'TA_AT
Cuts at: 160 1032 5077 160
Size: 872 4045 1606
Fragments arranged by size:
4045 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
AvaI C'yCGr_G
Cuts at: 1870 1943 3848 1870
Size: 73 1905 4545
Fragments arranged by size:
4545 1905 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2325 2358 2325
Size: 33 6490
BamHI G'GATC_C
Cuts at: 3853 4220 3853
Size: 367 6156
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2225 2905 3844 5299 618
Size: 325 217 627 438 680 939 1455 1842
Fragments arranged by size:
1842 1455 939 680 627 438 325 217
BanII G_rGCy'C
Cuts at: 1391 2867 3200 3842 1391
Size: 1476 333 642 4072
Fragments arranged by size:
4072 1476 642 333
BbeI G_GCGC'C
Cuts at: 2229 2229
Size: 6523
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2631 2948 3261 928
Size: 971 732 317 313 4190
Fragments arranged by size:
4190 971 732 317 313
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4714 4748 1903
Size: 34 2777 34 3678
Fragments arranged by size:
3678 2777 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4415 5625 4415
Size: 1210 5313
BclI T'GATC_A
Cuts at: 1548 3778 1548
Size: 2230 4293
BfrBI ATG'CAT
Cuts at: 2924 2924
Size: 6523
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5134 136
Size: 107 122 71 631 605 3462 1525
Fragments arranged by size:
3462 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2946 3370 6518 2946
Size: 424 3148 2951
Fragments arranged by size:
3148 2951 424
BlpI GC'TnA_GC
Cuts at: 3703 3940 3703
Size: 237 6286
Bme1580I G_kGCm'C
Cuts at: 1790 4584 5740 1790
Size: 2794 1156 2573
Fragments arranged by size:
2794 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6523
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2302 2573 4254
Size: 416 53 83 399 24 885 271 1681
Cuts at: 4254 5212 442
Size: 958 1753
Fragments arranged by size:
1753 1681 958 885 416 399 271 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6523
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2756 2756
Size: 6523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2751 3504 5183 1239
Size: 1512 753 1679 2579
Fragments arranged by size:
2579 1679 1512 753
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2430 4648 5513 5811 2430
Size: 2218 865 298 3142
Fragments arranged by size:
3142 2218 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5186 882
Size: 617 3687 2219
Fragments arranged by size:
3687 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4314 493
Size: 711 3110 2702
Fragments arranged by size:
3110 2702 711
BsaBI GATnn'nnATC
Cuts at: 6517 6517
Size: 6523
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2226 4330 4712 5347
Size: 53 83 186 936 693 2104 382 635
Cuts at: 5347 275
Size: 1451
Fragments arranged by size:
2104 1451 936 693 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2867 4956 5354 5628 5775 6186 2867
Size: 2089 398 274 147 411 3204
Fragments arranged by size:
3204 2089 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2084 2114 1178
Size: 30 165 30 681 30 5587
Fragments arranged by size:
5587 681 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 1186
Size: 877 5646
BseYI C'CCAG_C
Cuts at: 3276 5726 6222 3276
Size: 2450 496 3577
Fragments arranged by size:
3577 2450 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5950
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3973 4734 5762 1540
Size: 275 186 1972 761 1028 2301
Fragments arranged by size:
2301 1972 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2867 3200 4584 4669 5740 2867
Size: 333 1384 85 1071 3650
Fragments arranged by size:
3650 1384 1071 333 85
BsmI GAATG_Cn'
Cuts at: 2224 2566 3378 4034 4127 2224
Size: 342 812 656 93 4620
Fragments arranged by size:
4620 812 656 342 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2867 3200 3842 4584 4669 5740 1391
Size: 399 1077 333 642 742 85 1071 2174
Fragments arranged by size:
2174 1077 1071 742 642 399 333 85
BspEI T'CCGG_A
Cuts at: 2867 6186 2867
Size: 3319 3204
BspHI T'CATG_A
Cuts at: 3589 4412 6142 3589
Size: 823 1730 3970
Fragments arranged by size:
3970 1730 823
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3878 844
Size: 3034 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4410 1394
Size: 429 2587 3507
Fragments arranged by size:
3507 2587 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5018 5192 65
Size: 1183 3770 174 1396
Fragments arranged by size:
3770 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5167 5354 1700
Size: 110 3357 187 2869
Fragments arranged by size:
3357 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6523
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6523
BssSI C'ACGA_G
Cuts at: 4583 5595 4583
Size: 1012 5511
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6523
BstBI TT'CG_AA
Cuts at: 3208 3712 5327 3208
Size: 504 1615 4404
Fragments arranged by size:
4404 1615 504
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6523
BstYI r'GATC_y
Cuts at: 1972 2946 3370 3853 4220 4605 4622 6074 6160
Size: 974 424 483 367 385 17 1452 86
Cuts at: 6160 6172 6518 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 974 483 424 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2535 513
Size: 552 939 531 4501
Fragments arranged by size:
4501 939 552 531
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5784
Fragments arranged by size:
5784 683 56
BtsI GCAGTG_nn'
Cuts at: 4039 4833 4853 4039
Size: 794 20 5709
Fragments arranged by size:
5709 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6523
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2195 2230 529
Size: 35 1631 35 4822
Fragments arranged by size:
4822 1631 35 35
DraI TTT'AAA
Cuts at: 3079 3827 3079
Size: 748 5775
DraIII CAC_nnn'GTG
Cuts at: 2705 2705
Size: 6523
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5530 809
Size: 507 4214 1802
Fragments arranged by size:
4214 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3970 4859 6488
Size: 54 1425 50 275 18 2140 889 1629
Cuts at: 6488 8
Size: 43
Fragments arranged by size:
2140 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3970 1537
Size: 275 2158 4090
Fragments arranged by size:
4090 2158 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2979 3501 3714 3805 4453 1353
Size: 1626 522 213 91 648 3423
Fragments arranged by size:
3423 1626 648 522 213 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5110 5484 5630 387
Size: 1064 138 3521 374 146 1280
Fragments arranged by size:
3521 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2865 3198 2865
Size: 333 6190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2751 2923 3424 3504 4586 5183 5970
Size: 393 1119 172 501 80 1082 597 787
Cuts at: 5970 6238 1239
Size: 268 1524
Fragments arranged by size:
1524 1119 1082 787 597 501 393 268
172 80
EcoRI G'AATT_C
Cuts at: 3210 3714 3832 4210 3210
Size: 504 118 378 5523
Fragments arranged by size:
5523 504 378 118
FspI TGC'GCA
Cuts at: 5029 5029
Size: 6523
HaeII r_GCGC'y
Cuts at: 1678 2229 3283 5670 1678
Size: 551 1054 2387 2531
Fragments arranged by size:
2531 2387 1054 551
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2938 2970 3091 3123 3187
Size: 32 135 32 1366 32 121 32 64
Cuts at: 3187 3219 5164 5196 5238 5270 1373
Size: 32 1945 32 42 32 2626
Fragments arranged by size:
2626 1945 1366 135 121 64 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2845 3867 4113 669
Size: 988 281 907 1022 246 3079
Fragments arranged by size:
3079 1022 988 907 281 246
HindIII A'AGCT_T
Cuts at: 748 3455 748
Size: 2707 3816
HpaI GTT'AAC
Cuts at: 4113 4113
Size: 6523
KasI G'GCGC_C
Cuts at: 2225 2225
Size: 6523
KpnI G_GTAC'C
Cuts at: 2909 3848 2909
Size: 939 5584
MfeI C'AATT_G
Cuts at: 4122 4122
Size: 6523
MluI A'CGCG_T
Cuts at: 6399 6399
Size: 6523
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5637 5821 1305
Size: 4332 184 2007
Fragments arranged by size:
4332 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4701
Fragments arranged by size:
4701 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3163 3594 4481 4840 4999 518
Size: 648 1997 431 887 359 159 2042
Fragments arranged by size:
2042 1997 887 648 431 359 159
MspA1I CmG'CkG
Cuts at: 1606 3939 4616 5764 6009 1606
Size: 2333 677 1148 245 2120
Fragments arranged by size:
2333 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 2226 2226
Size: 6523
NcoI C'CATG_G
Cuts at: 513 1065 2004 2535 513
Size: 552 939 531 4501
Fragments arranged by size:
4501 939 552 531
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6523
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6523
NotI GC'GGCC_GC
Cuts at: 3970 3970
Size: 6523
NsiI A_TGCA'T
Cuts at: 2926 2926
Size: 6523
NspI r_CATG'y
Cuts at: 1132 1229 2769 2928 3881 5426 1132
Size: 97 1540 159 953 1545 2229
Fragments arranged by size:
2229 1545 1540 953 159 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6523
PciI A'CATG_T
Cuts at: 5422 5422
Size: 6523
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2351 1108
Size: 1243 5280
PfoI T'CCnGG_A
Cuts at: 2061 2519 2061
Size: 458 6065
PmeI GTTT'AAAC
Cuts at: 3827 3827
Size: 6523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2538 2570 4592 4624 6105 6137 2538
Size: 32 2022 32 1481 32 2924
Fragments arranged by size:
2924 2022 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3357 1613
Size: 1744 4779
PsiI TTA'TAA
Cuts at: 3246 3330 3768 4093 3246
Size: 84 438 325 5676
Fragments arranged by size:
5676 438 325 84
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3111 3143 3111
Size: 32 6491
PstI C_TGCA'G
Cuts at: 830 3875 830
Size: 3045 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6523
SacI G_AGCT'C
Cuts at: 2867 3200 2867
Size: 333 6190
SalI G'TCGA_C
Cuts at: 1936 3865 1936
Size: 1929 4594
SapI GCTCTTCn'nnn_
Cuts at: 3714 3714
Size: 6523
SbfI CC_TGCA'GG
Cuts at: 3875 3875
Size: 6523
ScaI AGT'ACT
Cuts at: 2657 3120 4771 6483 2657
Size: 463 1651 1712 2697
Fragments arranged by size:
2697 1712 1651 463
SfcI C'TryA_G
Cuts at: 826 1045 2086 3871 5006 5687 5878 826
Size: 219 1041 1785 1135 681 191 1471
Fragments arranged by size:
1785 1471 1135 1041 681 219 191
SfoI GGC'GCC
Cuts at: 2227 2227
Size: 6523
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6523
SmaI CCC'GGG
Cuts at: 1872 3850 1872
Size: 1978 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2445 4627 5528 5790 820
Size: 197 926 502 2182 901 262 1553
Fragments arranged by size:
2182 1553 926 901 502 262 197
SnaBI TAC'GTA
Cuts at: 493 4314 493
Size: 3821 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6523
SphI G_CATG'C
Cuts at: 1132 2769 3881 1132
Size: 1637 1112 3774
Fragments arranged by size:
3774 1637 1112
SspI AAT'ATT
Cuts at: 5 52 3764 4447 5
Size: 47 3712 683 2081
Fragments arranged by size:
3712 2081 683 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6523
StyI C'CwwG_G
Cuts at: 513 1065 2004 2417 2535 3962 513
Size: 552 939 413 118 1427 3074
Fragments arranged by size:
3074 1427 939 552 413 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4550 4567 4720 4905 5350 325
Size: 1255 2970 17 153 185 445 1498
Fragments arranged by size:
2970 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2655 3118 3223
Size: 274 80 33 51 1443 678 463 105
Cuts at: 3223 3475 4769 6481 96
Size: 252 1294 1712 138
Fragments arranged by size:
1712 1443 1294 678 463 274 252 138
105 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2084 2116 6483 6515 2084
Size: 32 4367 32 2092
Fragments arranged by size:
4367 2092 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6523
XbaI T'CTAG_A
Cuts at: 3859 3859
Size: 6523
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6523
XmaI C'CCGG_G
Cuts at: 1870 3848 1870
Size: 1978 4545
XmnI GAAnn'nnTTC
Cuts at: 2262 2592 3437 4652 2262
Size: 330 845 1215 4133
Fragments arranged by size:
4133 1215 845 330
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4331 276
Size: 53 83 186 936 2797 2468
Fragments arranged by size:
2797 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PsrI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbvCI Bpu10I BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI
FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI
PspOMI PspXI PvuII RsrII SacII SanDI SexAI SfiI
SrfI SwaI XcmI