Restriction map for
pFN21AB9747
(Circular) (Six-base) MAPSORT of: pFN21AB9747.seq Check: 4803 from: 1 to: 6340
pFN21AB9747 6340 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2166 4150 278
Size: 53 83 186 936 630 1984 2468
Fragments arranged by size:
2468 1984 936 630 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3423 3683 1937
Size: 1486 260 4594
Fragments arranged by size:
4594 1486 260
Acc65I G'GTAC_C
Cuts at: 3029 3661 3029
Size: 632 5708
AclI AA'CG_TT
Cuts at: 1563 4467 4840 1563
Size: 2904 373 3063
Fragments arranged by size:
3063 2904 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2537 3252 4403 5787 6055 1632
Size: 905 715 1151 1384 268 1917
Fragments arranged by size:
1917 1384 1151 905 715 268
AfeI AGC'GCT
Cuts at: 2064 2064
Size: 6340
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6143
AflIII A'CryG_T
Cuts at: 1203 2655 3258 5239 6216 1203
Size: 1452 603 1981 977 1327
Fragments arranged by size:
1981 1452 1327 977 603
AgeI A'CCGG_T
Cuts at: 5171 5171
Size: 6340
AhdI GACnn_n'nnGTC
Cuts at: 5069 5069
Size: 6340
AleI CACnn'nnGTG
Cuts at: 1166 2654 3417 1166
Size: 1488 763 4089
Fragments arranged by size:
4089 1488 763
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6340
AlwNI CAG_nnn'CTG
Cuts at: 2515 2959 4083 5655 2515
Size: 444 1124 1572 3200
Fragments arranged by size:
3200 1572 1124 444
ApaI G_GGCC'C
Cuts at: 2053 2060 2879 3201 3465 2053
Size: 7 819 322 264 4928
Fragments arranged by size:
4928 819 322 264 7
ApaLI G'TGCA_C
Cuts at: 4397 5553 4397
Size: 1156 5184
ApoI r'AATT_y
Cuts at: 1493 3649 3875 4027 6136 1493
Size: 2156 226 152 2109 1697
Fragments arranged by size:
2156 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4894 160
Size: 872 3862 1606
Fragments arranged by size:
3862 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
AvaI C'yCGr_G
Cuts at: 1870 1943 2053 2093 2146 2153 3200 3579 3665
Size: 73 110 40 53 7 1047 379 86
Cuts at: 3665 1870
Size: 4545
Fragments arranged by size:
4545 1047 379 110 86 73 53 40
7
BamHI G'GATC_C
Cuts at: 3670 4037 3670
Size: 367 5973
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2255 2888 3029 3661 5116
Size: 325 217 627 468 633 141 632 1455
Cuts at: 5116 618
Size: 1842
Fragments arranged by size:
1842 1455 633 632 627 468 325 217
141
BanII G_rGCy'C
Cuts at: 1391 2053 2060 2100 2232 2482 2879 3143 3201
Size: 662 7 40 132 250 397 264 58
Cuts at: 3201 3392 3465 3659 1391
Size: 191 73 194 4072
Fragments arranged by size:
4072 662 397 264 250 194 191 132
73 58 40 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5369
BbvCI CC'TCA_GC
Cuts at: 2120 2966 2120
Size: 846 5494
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4531 4565 1903
Size: 34 50 34 2510 34 3678
Fragments arranged by size:
3678 2510 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4232 5442 4232
Size: 1210 5130
BclI T'GATC_A
Cuts at: 1548 2265 1548
Size: 717 5623
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2048 2443 4951
Size: 107 122 71 631 605 376 395 2508
Cuts at: 4951 136
Size: 1525
Fragments arranged by size:
2508 1525 631 605 395 376 122 107
71
BglII A'GATC_T
Cuts at: 6335 6335
Size: 6340
BlpI GC'TnA_GC
Cuts at: 2424 3137 3248 3757 2424
Size: 713 111 509 5007
Fragments arranged by size:
5007 713 509 111
Bme1580I G_kGCm'C
Cuts at: 1790 2053 2060 2258 2879 2932 3201 3465 4401
Size: 263 7 198 621 53 269 264 936
Cuts at: 4401 5557 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 936 621 269 264 263 198
53 7
BmgBI CAC'GTC
Cuts at: 1318 2872 1318
Size: 1554 4786
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3433 4071 5029
Size: 416 53 83 399 24 2016 638 958
Cuts at: 5029 442
Size: 1753
Fragments arranged by size:
2016 1753 958 638 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2888 5000 1239
Size: 1649 2112 2579
Fragments arranged by size:
2579 2112 1649
Bpu10I CC'TnA_GC
Cuts at: 2120 2966 2120
Size: 846 5494
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2977 4465 5330 5628 2977
Size: 1488 865 298 3689
Fragments arranged by size:
3689 1488 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2506 2660 5003 882
Size: 617 1007 154 2343 2219
Fragments arranged by size:
2343 2219 1007 617 154
BsaAI yAC'GTr
Cuts at: 493 1204 2656 4131 493
Size: 711 1452 1475 2702
Fragments arranged by size:
2702 1475 1452 711
BsaBI GATnn'nnATC
Cuts at: 2267 2376 6334 2267
Size: 109 3958 2273
Fragments arranged by size:
3958 2273 109
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2163 4147 4529 5164
Size: 53 83 186 936 630 1984 382 635
Cuts at: 5164 275
Size: 1451
Fragments arranged by size:
1984 1451 936 635 630 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2866 4773 5171 5445 5592 6003 2866
Size: 1907 398 274 147 411 3203
Fragments arranged by size:
3203 1907 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6115
Fragments arranged by size:
6115 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 1186
Size: 865 5475
BseYI C'CCAG_C
Cuts at: 2059 2297 2712 2850 3393 3464 5543 6039 2059
Size: 238 415 138 543 71 2079 496 2360
Fragments arranged by size:
2360 2079 543 496 415 238 138 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2632 3317 1163
Size: 573 896 685 4186
Fragments arranged by size:
4186 896 685 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3790 4551 5579 1540
Size: 275 186 1789 761 1028 2301
Fragments arranged by size:
2301 1789 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2232 3392 4401 4486 5557 2232
Size: 1160 1009 85 1071 3015
Fragments arranged by size:
3015 1160 1071 1009 85
BsmI GAATG_Cn'
Cuts at: 3851 3944 3851
Size: 93 6247
BsmBI CGTCTCn'nnnn_
Cuts at: 3018 3018
Size: 6340
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 2060 2100 2232 2258 2482 2879
Size: 399 263 7 40 132 26 224 397
Cuts at: 2879 2932 3143 3201 3392 3465 3659 4401 4486
Size: 53 211 58 191 73 194 742 85
Cuts at: 4486 5557 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 397 263 224 211
194 191 132 85 73 58 53 40
26 7
BspEI T'CCGG_A
Cuts at: 6003 6003
Size: 6340
BspHI T'CATG_A
Cuts at: 2826 4229 5959 2826
Size: 1403 1730 3207
Fragments arranged by size:
3207 1730 1403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2781 2967 3240 3695 844
Size: 1937 186 273 455 3489
Fragments arranged by size:
3489 1937 455 273 186
BsrBI CCG'CTC
Cuts at: 1394 1823 3383 3457 4227 1394
Size: 429 1560 74 770 3507
Fragments arranged by size:
3507 1560 770 429 74
BsrDI GCAATG_nn'
Cuts at: 65 1248 4835 5009 65
Size: 1183 3587 174 1396
Fragments arranged by size:
3587 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4984 5171 1700
Size: 110 3174 187 2869
Fragments arranged by size:
3174 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6340
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6340
BssSI C'ACGA_G
Cuts at: 2246 2918 4400 5412 2246
Size: 672 1482 1012 3174
Fragments arranged by size:
3174 1482 1012 672
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2959 794
Size: 2165 4175
BstBI TT'CG_AA
Cuts at: 5144 5144
Size: 6340
BstEII G'GTnAC_C
Cuts at: 1184 3160 1184
Size: 1976 4364
BstYI r'GATC_y
Cuts at: 1972 3670 4037 4422 4439 5891 5977 5989 6335
Size: 1698 367 385 17 1452 86 12 346
Cuts at: 6335 1972
Size: 1977
Fragments arranged by size:
1977 1698 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2181 2585 3362 513
Size: 552 1116 404 777 3491
Fragments arranged by size:
3491 1116 777 552 404
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2363 487
Size: 683 56 1137 4464
Fragments arranged by size:
4464 1137 683 56
BtsI GCAGTG_nn'
Cuts at: 2637 2764 3007 3160 3856 4650 4670 2637
Size: 127 243 153 696 794 20 4307
Fragments arranged by size:
4307 794 696 243 153 127 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6340
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6305
DraI TTT'AAA
Cuts at: 3644 3644
Size: 6340
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3271 5347 809
Size: 507 1955 2076 1802
Fragments arranged by size:
2076 1955 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2548 3787 4676
Size: 54 1425 50 275 18 718 1239 889
Cuts at: 4676 6305 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1239 889 718 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3787 1537
Size: 275 1975 4090
Fragments arranged by size:
4090 1975 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3005 3085 4270 1353
Size: 1652 80 1185 3423
Fragments arranged by size:
3423 1652 1185 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4927 5301 5447 387
Size: 1064 138 3338 374 146 1280
Fragments arranged by size:
3338 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2230 3390 2230
Size: 1160 5180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2537 2888 3252 4403 5000 5787 6055
Size: 393 905 351 364 1151 597 787 268
Cuts at: 6055 1239
Size: 1524
Fragments arranged by size:
1524 1151 905 787 597 393 364 351
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2472 2472
Size: 6340
EcoO109I rG'GnC_Cy
Cuts at: 2020 2050 2150 2876 2956 3197 3461 2020
Size: 30 100 726 80 241 264 4899
Fragments arranged by size:
4899 726 264 241 100 80 30
EcoRI G'AATT_C
Cuts at: 3649 4027 3649
Size: 378 5962
FspI TGC'GCA
Cuts at: 4846 4846
Size: 6340
HaeII r_GCGC'y
Cuts at: 1678 2066 2114 3471 5487 1678
Size: 388 48 1357 2016 2531
Fragments arranged by size:
2531 2016 1357 388 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4981 5013 5055 5087 1373
Size: 32 135 32 3409 32 42 32 2626
Fragments arranged by size:
3409 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3684 3930 669
Size: 988 281 1746 246 3079
Fragments arranged by size:
3079 1746 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6340
HpaI GTT'AAC
Cuts at: 3930 3930
Size: 6340
KpnI G_GTAC'C
Cuts at: 3033 3665 3033
Size: 632 5708
MfeI C'AATT_G
Cuts at: 3939 3939
Size: 6340
MluI A'CGCG_T
Cuts at: 6216 6216
Size: 6340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5454 5638 1305
Size: 4149 184 2007
Fragments arranged by size:
4149 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2550 10
Size: 54 1425 343 718 3800
Fragments arranged by size:
3800 1425 718 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2207 2261 2321 2654 2681 3353 3356
Size: 648 1041 54 60 333 27 672 3
Cuts at: 3356 3417 3534 4298 4657 4816 518
Size: 61 117 764 359 159 2042
Fragments arranged by size:
2042 1041 764 672 648 359 333 159
117 61 60 54 27 3
MspA1I CmG'CkG
Cuts at: 1606 2124 2183 2497 2587 2716 3247 3364 3756
Size: 518 59 314 90 129 531 117 392
Cuts at: 3756 4433 5581 5826 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 531 518 392 314 245
129 117 90 59
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5788
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6340
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6340
NotI GC'GGCC_GC
Cuts at: 3787 3787
Size: 6340
NspI r_CATG'y
Cuts at: 1132 1229 3180 3262 3698 5243 1132
Size: 97 1951 82 436 1545 2229
Fragments arranged by size:
2229 1951 1545 436 97 82
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6340
PciI A'CATG_T
Cuts at: 3258 5239 3258
Size: 1981 4359
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6340
PmeI GTTT'AAAC
Cuts at: 3644 3644
Size: 6340
PmlI CAC'GTG
Cuts at: 2656 2656
Size: 6340
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4409 4441 5922 5954 4409
Size: 32 1481 32 4795
Fragments arranged by size:
4795 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2150 2956 2150
Size: 806 5534
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6340
PsiI TTA'TAA
Cuts at: 3910 3910
Size: 6340
PspOMI G'GGCC_C
Cuts at: 2049 2056 2875 3197 3461 2049
Size: 7 819 322 264 4928
Fragments arranged by size:
4928 819 322 264 7
PstI C_TGCA'G
Cuts at: 830 2013 2793 3155 3692 830
Size: 1183 780 362 537 3478
Fragments arranged by size:
3478 1183 780 537 362
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6340
PvuII CAG'CTG
Cuts at: 2497 2716 2497
Size: 219 6121
SacI G_AGCT'C
Cuts at: 2232 3392 2232
Size: 1160 5180
SacII CC_GC'GG
Cuts at: 2184 2588 3365 2184
Size: 404 777 5159
Fragments arranged by size:
5159 777 404
SalI G'TCGA_C
Cuts at: 1936 3682 1936
Size: 1746 4594
SanDI GG'GwC_CC
Cuts at: 2150 2150
Size: 6340
SbfI CC_TGCA'GG
Cuts at: 3692 3692
Size: 6340
ScaI AGT'ACT
Cuts at: 4588 6300 4588
Size: 1712 4628
SfcI C'TryA_G
Cuts at: 826 1045 2009 2789 3151 3289 3688 4823 5504
Size: 219 964 780 362 138 399 1135 681
Cuts at: 5504 5695 826
Size: 191 1471
Fragments arranged by size:
1471 1135 964 780 681 399 362 219
191 138
SfiI GGCCn_nnn'nGGCC
Cuts at: 2048 2048
Size: 6340
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6340
SmaI CCC'GGG
Cuts at: 1872 2055 2148 2155 3667 1872
Size: 183 93 7 1512 4545
Fragments arranged by size:
4545 1512 183 93 7
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2992 4444 5345 5607 820
Size: 197 926 1049 1452 901 262 1553
Fragments arranged by size:
1553 1452 1049 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4131 493
Size: 3638 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6340
SphI G_CATG'C
Cuts at: 1132 3698 1132
Size: 2566 3774
SspI AAT'ATT
Cuts at: 5 52 4264 5
Size: 47 4212 2081
Fragments arranged by size:
4212 2081 47
StuI AGG'CCT
Cuts at: 1517 2042 1517
Size: 525 5815
StyI C'CwwG_G
Cuts at: 513 1065 2742 3499 3779 513
Size: 552 1677 757 280 3074
Fragments arranged by size:
3074 1677 757 552 280
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2666 2726 2906 2996 4367 4384 4537
Size: 1255 1086 60 180 90 1371 17 153
Cuts at: 4537 4722 5167 325
Size: 185 445 1498
Fragments arranged by size:
1498 1371 1255 1086 445 185 180 153
90 60 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3183 4586 6298
Size: 274 80 33 51 1443 1206 1403 1712
Cuts at: 6298 96
Size: 138
Fragments arranged by size:
1712 1443 1403 1206 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6300 6332 6300
Size: 32 6308
Tth111I GACn'n_nGTC
Cuts at: 1316 2165 2360 1316
Size: 849 195 5296
Fragments arranged by size:
5296 849 195
XbaI T'CTAG_A
Cuts at: 3676 3676
Size: 6340
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2869 2869
Size: 6340
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6340
XmaI C'CCGG_G
Cuts at: 1870 2053 2146 2153 3665 1870
Size: 183 93 7 1512 4545
Fragments arranged by size:
4545 1512 183 93 7
XmnI GAAnn'nnTTC
Cuts at: 2240 4469 2240
Size: 2229 4111
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2164 4148 276
Size: 53 83 186 936 630 1984 2468
Fragments arranged by size:
2468 1984 936 630 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PspXI PsrI RsrII SapI SexAI SfoI SrfI
SwaI