Restriction map for
pFN21AB9746
(Circular) (Six-base) MAPSORT of: pFN21AB9746.seq Check: 2520 from: 1 to: 6331
pFN21AB9746 6331 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4141 278
Size: 53 83 186 936 2605 2468
Fragments arranged by size:
2605 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2107 3674 1937
Size: 170 1567 4594
Fragments arranged by size:
4594 1567 170
Acc65I G'GTAC_C
Cuts at: 3652 3652
Size: 6331
AclI AA'CG_TT
Cuts at: 1563 4458 4831 1563
Size: 2895 373 3063
Fragments arranged by size:
3063 2895 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2551 2887 3139 3307 4394 5778 6046 1632
Size: 919 336 252 168 1087 1384 268 1917
Fragments arranged by size:
1917 1384 1087 919 336 268 252 168
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6134
AflIII A'CryG_T
Cuts at: 1203 5230 6207 1203
Size: 4027 977 1327
Fragments arranged by size:
4027 1327 977
AgeI A'CCGG_T
Cuts at: 5162 5162
Size: 6331
AhdI GACnn_n'nnGTC
Cuts at: 2148 3240 5060 2148
Size: 1092 1820 3419
Fragments arranged by size:
3419 1820 1092
AleI CACnn'nnGTG
Cuts at: 1166 2358 2526 3450 3534 1166
Size: 1192 168 924 84 3963
Fragments arranged by size:
3963 1192 924 168 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6331
AlwNI CAG_nnn'CTG
Cuts at: 4074 5646 4074
Size: 1572 4759
ApaLI G'TGCA_C
Cuts at: 3161 4388 5544 3161
Size: 1227 1156 3948
Fragments arranged by size:
3948 1227 1156
ApoI r'AATT_y
Cuts at: 1493 2349 2601 2685 2853 3189 3357 3640 3866
Size: 856 252 84 168 336 168 283 226
Cuts at: 3866 4018 6127 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 856 336 283 252 226 168
168 152 84
AseI AT'TA_AT
Cuts at: 160 1032 4885 160
Size: 872 3853 1606
Fragments arranged by size:
3853 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6331
AvaI C'yCGr_G
Cuts at: 1870 1943 3656 1870
Size: 73 1713 4545
Fragments arranged by size:
4545 1713 73
BamHI G'GATC_C
Cuts at: 3021 3661 4028 3021
Size: 640 367 5324
Fragments arranged by size:
5324 640 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3652 5107 618
Size: 325 217 627 1865 1455 1842
Fragments arranged by size:
1865 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3650 1391
Size: 2259 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2941 3575 928
Size: 971 1042 634 3684
Fragments arranged by size:
3684 1042 971 634
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4522 4556 1903
Size: 34 2585 34 3678
Fragments arranged by size:
3678 2585 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4223 5433 4223
Size: 1210 5121
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6331
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4942 136
Size: 107 122 71 631 605 3270 1525
Fragments arranged by size:
3270 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6326 6326
Size: 6331
BlpI GC'TnA_GC
Cuts at: 2326 2673 3748 2326
Size: 347 1075 4909
Fragments arranged by size:
4909 1075 347
Bme1580I G_kGCm'C
Cuts at: 1790 3165 4392 5548 1790
Size: 1375 1227 1156 2573
Fragments arranged by size:
2573 1375 1227 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6331
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2451 3039 3375
Size: 416 53 83 399 24 1034 588 336
Cuts at: 3375 4062 5020 442
Size: 687 958 1753
Fragments arranged by size:
1753 1034 958 687 588 416 399 336
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3135 4991 1239
Size: 1896 1856 2579
Fragments arranged by size:
2579 1896 1856
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4456 5321 5619 4456
Size: 865 298 5168
Fragments arranged by size:
5168 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4994 882
Size: 617 3495 2219
Fragments arranged by size:
3495 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2120 3166 4122 493
Size: 711 916 1046 956 2702
Fragments arranged by size:
2702 1046 956 916 711
BsaBI GATnn'nnATC
Cuts at: 6325 6325
Size: 6331
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4138 4520 5155 275
Size: 53 83 186 936 2605 382 635 1451
Fragments arranged by size:
2605 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4764 5162 5436 5583 5994 4764
Size: 398 274 147 411 5101
Fragments arranged by size:
5101 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6106
Fragments arranged by size:
6106 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2564 1186
Size: 1378 4953
BseYI C'CCAG_C
Cuts at: 5534 6030 5534
Size: 496 5835
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5758
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3781 4542 5570 1540
Size: 275 186 1780 761 1028 2301
Fragments arranged by size:
2301 1780 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3165 4392 4477 5548 3165
Size: 1227 85 1071 3948
Fragments arranged by size:
3948 1227 1071 85
BsmI GAATG_Cn'
Cuts at: 2633 2894 3842 3935 2633
Size: 261 948 93 5029
Fragments arranged by size:
5029 948 261 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3165 3650 4392 4477 5548 1391
Size: 399 1375 485 742 85 1071 2174
Fragments arranged by size:
2174 1375 1071 742 485 399 85
BspEI T'CCGG_A
Cuts at: 5994 5994
Size: 6331
BspHI T'CATG_A
Cuts at: 4220 5950 4220
Size: 1730 4601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3686 844
Size: 2842 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4218 1394
Size: 429 2395 3507
Fragments arranged by size:
3507 2395 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2390 4826 5000 65
Size: 1183 1142 2436 174 1396
Fragments arranged by size:
2436 1396 1183 1142 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4975 5162 1700
Size: 110 3165 187 2869
Fragments arranged by size:
3165 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3259 96
Size: 3163 3168
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6331
BssSI C'ACGA_G
Cuts at: 4391 5403 4391
Size: 1012 5319
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2889 794
Size: 2095 4236
BstBI TT'CG_AA
Cuts at: 5135 5135
Size: 6331
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6331
BstYI r'GATC_y
Cuts at: 1972 3021 3661 4028 4413 4430 5882 5968 5980
Size: 1049 640 367 385 17 1452 86 12
Cuts at: 5980 6326 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1049 640 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4840
Fragments arranged by size:
4840 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5592
Fragments arranged by size:
5592 683 56
BtsI GCAGTG_nn'
Cuts at: 3847 4641 4661 3847
Size: 794 20 5517
Fragments arranged by size:
5517 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6331
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2620 2655 3544 3579 529
Size: 35 2056 35 889 35 3281
Fragments arranged by size:
3281 2056 889 35 35 35
DraI TTT'AAA
Cuts at: 3550 3635 3550
Size: 85 6246
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5338 809
Size: 507 4022 1802
Fragments arranged by size:
4022 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3778 4667 6296
Size: 54 1425 50 275 18 1948 889 1629
Cuts at: 6296 8
Size: 43
Fragments arranged by size:
1948 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3778 1537
Size: 275 1966 4090
Fragments arranged by size:
4090 1966 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4261 1353
Size: 2908 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4918 5292 5438 387
Size: 1064 138 3329 374 146 1280
Fragments arranged by size:
3329 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2551 2887 3135 3139 3307 4394 4991
Size: 393 919 336 248 4 168 1087 597
Cuts at: 4991 5778 6046 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1087 919 787 597 393 336 268
248 168 4
EcoRI G'AATT_C
Cuts at: 2349 2601 2685 2853 3357 3640 4018 2349
Size: 252 84 168 504 283 378 4662
Fragments arranged by size:
4662 504 378 283 252 168 84
FspI TGC'GCA
Cuts at: 4837 4837
Size: 6331
HaeII r_GCGC'y
Cuts at: 1678 5478 1678
Size: 3800 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2134 2166 2232 2264 3226
Size: 32 135 32 562 32 66 32 962
Cuts at: 3226 3258 4972 5004 5046 5078 1373
Size: 32 1714 32 42 32 2626
Fragments arranged by size:
2626 1714 962 562 135 66 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3675 3921 669
Size: 988 281 1737 246 3079
Fragments arranged by size:
3079 1737 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2478 2498 748
Size: 1730 20 4581
Fragments arranged by size:
4581 1730 20
HpaI GTT'AAC
Cuts at: 3921 3921
Size: 6331
KpnI G_GTAC'C
Cuts at: 3656 3656
Size: 6331
MfeI C'AATT_G
Cuts at: 3930 3930
Size: 6331
MluI A'CGCG_T
Cuts at: 6207 6207
Size: 6331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2007 5445 5629 1305
Size: 702 3438 184 2007
Fragments arranged by size:
3438 2007 702 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4509
Fragments arranged by size:
4509 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2122 2190 2274 2358 2374 2458 2526
Size: 648 956 68 84 84 16 84 68
Cuts at: 2526 2610 2778 3198 3438 3450 3534 3618 4289
Size: 84 168 420 240 12 84 84 671
Cuts at: 4289 4648 4807 518
Size: 359 159 2042
Fragments arranged by size:
2042 956 671 648 420 359 240 168
159 84 84 84 84 84 84 68
68 16 12
MspA1I CmG'CkG
Cuts at: 1606 3523 3747 4424 5572 5817 1606
Size: 1917 224 677 1148 245 2120
Fragments arranged by size:
2120 1917 1148 677 245 224
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4840
Fragments arranged by size:
4840 939 552
NdeI CA'TA_TG
Cuts at: 387 2455 3615 387
Size: 2068 1160 3103
Fragments arranged by size:
3103 2068 1160
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6331
NotI GC'GGCC_GC
Cuts at: 3778 3778
Size: 6331
NspI r_CATG'y
Cuts at: 1132 1229 2273 3689 5234 1132
Size: 97 1044 1416 1545 2229
Fragments arranged by size:
2229 1545 1416 1044 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6331
PciI A'CATG_T
Cuts at: 5230 5230
Size: 6331
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6331
PmeI GTTT'AAAC
Cuts at: 3635 3635
Size: 6331
PmlI CAC'GTG
Cuts at: 2120 2120
Size: 6331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4400 4432 5913 5945 4400
Size: 32 1481 32 4786
Fragments arranged by size:
4786 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6331
PsiI TTA'TAA
Cuts at: 3901 3901
Size: 6331
PstI C_TGCA'G
Cuts at: 830 3683 830
Size: 2853 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6331
SalI G'TCGA_C
Cuts at: 1936 3673 1936
Size: 1737 4594
SbfI CC_TGCA'GG
Cuts at: 3683 3683
Size: 6331
ScaI AGT'ACT
Cuts at: 2518 2992 4579 6291 2518
Size: 474 1587 1712 2558
Fragments arranged by size:
2558 1712 1587 474
SexAI A'CCwGG_T
Cuts at: 2583 2583
Size: 6331
SfcI C'TryA_G
Cuts at: 826 1045 3679 4814 5495 5686 826
Size: 219 2634 1135 681 191 1471
Fragments arranged by size:
2634 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6331
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6331
SmaI CCC'GGG
Cuts at: 1872 3658 1872
Size: 1786 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4435 5336 5598 820
Size: 197 926 2492 901 262 1553
Fragments arranged by size:
2492 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4122 493
Size: 3629 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6331
SphI G_CATG'C
Cuts at: 1132 3689 1132
Size: 2557 3774
SspI AAT'ATT
Cuts at: 5 52 4255 5
Size: 47 4203 2081
Fragments arranged by size:
4203 2081 47
StuI AGG'CCT
Cuts at: 1517 2312 2564 2648 2816 2984 3152 3320 3488
Size: 795 252 84 168 168 168 168 168
Cuts at: 3488 1517
Size: 4360
Fragments arranged by size:
4360 795 252 168 168 168 168 168
84
StyI C'CwwG_G
Cuts at: 513 1065 2004 3770 513
Size: 552 939 1766 3074
Fragments arranged by size:
3074 1766 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2532 4358 4375 4528 4713 5158 325
Size: 1255 952 1826 17 153 185 445 1498
Fragments arranged by size:
1826 1498 1255 952 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2422 2516 2990
Size: 274 80 33 51 1443 445 94 474
Cuts at: 2990 3259 4577 6289 96
Size: 269 1318 1712 138
Fragments arranged by size:
1712 1443 1318 474 445 274 269 138
94 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6291 6323 6291
Size: 32 6299
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6331
XbaI T'CTAG_A
Cuts at: 3667 3667
Size: 6331
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6331
XmaI C'CCGG_G
Cuts at: 1870 3656 1870
Size: 1786 4545
XmnI GAAnn'nnTTC
Cuts at: 2564 2732 2900 4460 2564
Size: 168 168 1560 4435
Fragments arranged by size:
4435 1560 168 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4139 276
Size: 53 83 186 936 2605 2468
Fragments arranged by size:
2605 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI