Restriction map for
pFN21AB9744
(Circular) (Six-base) MAPSORT of: pFN21AB9744.seq Check: 7844 from: 1 to: 6301
pFN21AB9744 6301 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3374 3374
Size: 6301
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3528 4111 278
Size: 53 83 186 936 1992 583 2468
Fragments arranged by size:
2468 1992 936 583 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3644 1937
Size: 1707 4594
Acc65I G'GTAC_C
Cuts at: 3622 3622
Size: 6301
AclI AA'CG_TT
Cuts at: 1563 4428 4801 1563
Size: 2865 373 3063
Fragments arranged by size:
3063 2865 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2492 2582 2666 2852 2894 4364 5748 6016
Size: 860 90 84 186 42 1470 1384 268
Cuts at: 6016 1632
Size: 1917
Fragments arranged by size:
1917 1470 1384 860 268 186 90 84
42
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6104
AflIII A'CryG_T
Cuts at: 1203 5200 6177 1203
Size: 3997 977 1327
Fragments arranged by size:
3997 1327 977
AgeI A'CCGG_T
Cuts at: 5132 5132
Size: 6301
AhdI GACnn_n'nnGTC
Cuts at: 5030 5030
Size: 6301
AleI CACnn'nnGTG
Cuts at: 1166 3572 1166
Size: 2406 3895
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6301
AlwNI CAG_nnn'CTG
Cuts at: 2452 3055 3167 4044 5616 2452
Size: 603 112 877 1572 3137
Fragments arranged by size:
3137 1572 877 603 112
ApaI G_GGCC'C
Cuts at: 2590 2984 2590
Size: 394 5907
ApaLI G'TGCA_C
Cuts at: 2774 4358 5514 2774
Size: 1584 1156 3561
Fragments arranged by size:
3561 1584 1156
ApoI r'AATT_y
Cuts at: 1493 3610 3836 3988 6097 1493
Size: 2117 226 152 2109 1697
Fragments arranged by size:
2117 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4855 160
Size: 872 3823 1606
Fragments arranged by size:
3823 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
AvaI C'yCGr_G
Cuts at: 1870 1943 2158 2406 2465 2861 3101 3626 1870
Size: 73 215 248 59 396 240 525 4545
Fragments arranged by size:
4545 525 396 248 240 215 73 59
BamHI G'GATC_C
Cuts at: 2476 3631 3998 2476
Size: 1155 367 4779
Fragments arranged by size:
4779 1155 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2999 3010 3021 3262 3622
Size: 325 217 627 1212 11 11 241 360
Cuts at: 3622 5077 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1212 627 360 325 241 217
11 11
BanII G_rGCy'C
Cuts at: 1391 2590 2768 2912 2984 3620 1391
Size: 1199 178 144 72 636 4072
Fragments arranged by size:
4072 1199 636 178 144 72
BbeI G_GCGC'C
Cuts at: 3266 3266
Size: 6301
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2029 2542 2570 928
Size: 971 130 513 28 4659
Fragments arranged by size:
4659 971 513 130 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3484 3512 3518 3546 4492 4526 1903
Size: 34 1547 28 6 28 946 34 3678
Fragments arranged by size:
3678 1547 946 34 34 28 28 6
BciVI GTATCCnnnnn_n'
Cuts at: 4193 5403 4193
Size: 1210 5091
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6301
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2663 2748 3206
Size: 107 122 71 631 605 991 85 458
Cuts at: 3206 3356 4912 136
Size: 150 1556 1525
Fragments arranged by size:
1556 1525 991 631 605 458 150 122
107 85 71
BglII A'GATC_T
Cuts at: 6296 6296
Size: 6301
BlpI GC'TnA_GC
Cuts at: 2015 3056 3718 2015
Size: 1041 662 4598
Fragments arranged by size:
4598 1041 662
Bme1580I G_kGCm'C
Cuts at: 1790 2590 2778 2984 3026 3179 4362 5518 1790
Size: 800 188 206 42 153 1183 1156 2573
Fragments arranged by size:
2573 1183 1156 800 206 188 153 42
BmgBI CAC'GTC
Cuts at: 1318 2346 1318
Size: 1028 5273
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2420 2673 3189
Size: 416 53 83 399 24 1003 253 516
Cuts at: 3189 3309 4032 4990 442
Size: 120 723 958 1753
Fragments arranged by size:
1753 1003 958 723 516 416 399 253
120 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3248 1056
Size: 2192 4109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 2898 3047 4961 1239
Size: 866 793 149 1914 2579
Fragments arranged by size:
2579 1914 866 793 149
Bpu10I CC'TnA_GC
Cuts at: 2057 2057
Size: 6301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2273 4426 5291 5589 2273
Size: 2153 865 298 2985
Fragments arranged by size:
2985 2153 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2411 3116 4964 882
Size: 617 912 705 1848 2219
Fragments arranged by size:
2219 1848 912 705 617
BsaAI yAC'GTr
Cuts at: 493 1204 3511 4092 493
Size: 711 2307 581 2702
Fragments arranged by size:
2702 2307 711 581
BsaBI GATnn'nnATC
Cuts at: 6295 6295
Size: 6301
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3263 3525 4108 4490
Size: 53 83 186 936 1730 262 583 382
Cuts at: 4490 5125 275
Size: 635 1451
Fragments arranged by size:
1730 1451 936 635 583 382 262 186
83 53
BsaWI w'CCGG_w
Cuts at: 2211 2484 2787 4734 5132 5406 5553 5964 2211
Size: 273 303 1947 398 274 147 411 2548
Fragments arranged by size:
2548 1947 411 398 303 274 273 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3404 3434 1178
Size: 30 165 30 2001 30 4045
Fragments arranged by size:
4045 2001 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2156 2323 2344 3404 1186
Size: 838 132 167 21 1060 4083
Fragments arranged by size:
4083 1060 838 167 132 21
BseYI C'CCAG_C
Cuts at: 2130 2899 2939 3017 3033 3160 3434 5504 6000
Size: 769 40 78 16 127 274 2070 496
Cuts at: 6000 2130
Size: 2431
Fragments arranged by size:
2431 2070 769 496 274 127 78 40
16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2225 1163
Size: 573 489 5239
Fragments arranged by size:
5239 573 489
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2743 3751 4512 5540 1540
Size: 275 186 742 1008 761 1028 2301
Fragments arranged by size:
2301 1028 1008 761 742 275 186
BsiHKAI G_wGCw'C
Cuts at: 2768 2778 3510 4362 4447 5518 2768
Size: 10 732 852 85 1071 3551
Fragments arranged by size:
3551 1071 852 732 85 10
BsmI GAATG_Cn'
Cuts at: 3812 3905 3812
Size: 93 6208
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2590 2768 2778 2912 2984 3026 3179
Size: 399 800 178 10 134 72 42 153
Cuts at: 3179 3510 3620 4362 4447 5518 1391
Size: 331 110 742 85 1071 2174
Fragments arranged by size:
2174 1071 800 742 399 331 178 153
134 110 85 72 42 10
BspEI T'CCGG_A
Cuts at: 2484 5964 2484
Size: 3480 2821
BspHI T'CATG_A
Cuts at: 4190 5920 4190
Size: 1730 4571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2023 2811 3374 3656 844
Size: 1179 788 563 282 3489
Fragments arranged by size:
3489 1179 788 563 282
BsrBI CCG'CTC
Cuts at: 1394 1823 4188 1394
Size: 429 2365 3507
Fragments arranged by size:
3507 2365 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4796 4970 65
Size: 1183 3548 174 1396
Fragments arranged by size:
3548 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2238 2274 4945 5132 1700
Size: 110 428 36 2671 187 2869
Fragments arranged by size:
2869 2671 428 187 110 36
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6301
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6301
BssSI C'ACGA_G
Cuts at: 4361 5373 4361
Size: 1012 5289
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6301
BstBI TT'CG_AA
Cuts at: 5105 5105
Size: 6301
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6301
BstXI CCAn_nnnn'nTGG
Cuts at: 2686 2824 3259 3574 2686
Size: 138 435 315 5413
Fragments arranged by size:
5413 435 315 138
BstYI r'GATC_y
Cuts at: 1972 2448 2476 2578 3631 3998 4383 4400 5852
Size: 476 28 102 1053 367 385 17 1452
Cuts at: 5852 5938 5950 6296 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1053 476 385 367 346 102
86 28 17 12
Bsu36I CC'TnA_GG
Cuts at: 3590 3590
Size: 6301
BtgI C'CryG_G
Cuts at: 513 1065 2004 2685 2995 513
Size: 552 939 681 310 3819
Fragments arranged by size:
3819 939 681 552 310
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5562
Fragments arranged by size:
5562 683 56
BtsI GCAGTG_nn'
Cuts at: 2253 3264 3310 3365 3528 3817 4611 4631 2253
Size: 1011 46 55 163 289 794 20 3923
Fragments arranged by size:
3923 1011 794 289 163 55 46 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6301
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6266
DraI TTT'AAA
Cuts at: 3605 3605
Size: 6301
DraIII CAC_nnn'GTG
Cuts at: 2428 2428
Size: 6301
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5308 809
Size: 507 3992 1802
Fragments arranged by size:
3992 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2240 2457 2550
Size: 54 1425 50 275 18 410 217 93
Cuts at: 2550 2592 2688 2706 2727 2740 3150 3360 3748
Size: 42 96 18 21 13 410 210 388
Cuts at: 3748 4637 6266 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 410 410 388 275 217
210 96 93 54 50 43 42 21
18 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 2740 3748 1537
Size: 275 928 1008 4090
Fragments arranged by size:
4090 1008 928 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2210 2341 2362 2364 4231 1353
Size: 857 131 21 2 1867 3423
Fragments arranged by size:
3423 1867 857 131 21 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3412 4888 5262 5408 387
Size: 1064 138 1823 1476 374 146 1280
Fragments arranged by size:
1823 1476 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2766 2766
Size: 6301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2492 2582 2666 2852 2894 2898
Size: 393 473 387 90 84 186 42 4
Cuts at: 2898 3047 4364 4961 5748 6016 1239
Size: 149 1317 597 787 268 1524
Fragments arranged by size:
1524 1317 787 597 473 393 387 268
186 149 90 84 42 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2552 2717 2552
Size: 165 6136
EcoO109I rG'GnC_Cy
Cuts at: 2402 2586 2587 2935 2980 2981 3029 2402
Size: 184 1 348 45 1 48 5674
Fragments arranged by size:
5674 348 184 48 45 1 1
EcoRI G'AATT_C
Cuts at: 3610 3988 3610
Size: 378 5923
FseI GG_CCGG'CC
Cuts at: 2242 2242
Size: 6301
FspI TGC'GCA
Cuts at: 4807 4807
Size: 6301
HaeII r_GCGC'y
Cuts at: 1678 2281 2622 2831 3266 5448 1678
Size: 603 341 209 435 2182 2531
Fragments arranged by size:
2531 2182 603 435 341 209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2291 2323 3404 3410 3436
Size: 32 135 32 719 32 1081 6 26
Cuts at: 3436 3442 4942 4974 5016 5048 1373
Size: 6 1500 32 42 32 2626
Fragments arranged by size:
2626 1500 1081 719 135 42 32 32
32 32 32 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3645 3891 669
Size: 988 281 1707 246 3079
Fragments arranged by size:
3079 1707 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6301
HpaI GTT'AAC
Cuts at: 3891 3891
Size: 6301
KasI G'GCGC_C
Cuts at: 3262 3262
Size: 6301
KpnI G_GTAC'C
Cuts at: 3626 3626
Size: 6301
MfeI C'AATT_G
Cuts at: 3900 3900
Size: 6301
MluI A'CGCG_T
Cuts at: 6177 6177
Size: 6301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3435 5415 5599 1305
Size: 2130 1980 184 2007
Fragments arranged by size:
2130 2007 1980 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2459 2552 2690 2708 2729
Size: 54 1425 343 627 93 138 18 21
Cuts at: 2729 10
Size: 3582
Fragments arranged by size:
3582 1425 627 343 138 93 54 21
18
MslI CAynn'nnrTG
Cuts at: 518 1166 2258 2994 3572 4259 4618 4777 518
Size: 648 1092 736 578 687 359 159 2042
Fragments arranged by size:
2042 1092 736 687 648 578 359 159
MspA1I CmG'CkG
Cuts at: 1606 2130 2757 2943 3204 3717 4394 5542 5787
Size: 524 627 186 261 513 677 1148 245
Cuts at: 5787 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 627 524 513 261 245
186
NaeI GCC'GGC
Cuts at: 2240 2276 2240
Size: 36 6265
NarI GG'CG_CC
Cuts at: 3263 3263
Size: 6301
NcoI C'CATG_G
Cuts at: 513 1065 2004 2685 2995 513
Size: 552 939 681 310 3819
Fragments arranged by size:
3819 939 681 552 310
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6301
NgoMIV G'CCGG_C
Cuts at: 2238 2274 2238
Size: 36 6265
NheI G'CTAG_C
Cuts at: 1052 3244 1052
Size: 2192 4109
NotI GC'GGCC_GC
Cuts at: 3748 3748
Size: 6301
NspI r_CATG'y
Cuts at: 1132 1229 3100 3659 5204 1132
Size: 97 1871 559 1545 2229
Fragments arranged by size:
2229 1871 1545 559 97
PasI CC'CwG_GG
Cuts at: 1348 3167 1348
Size: 1819 4482
PciI A'CATG_T
Cuts at: 5200 5200
Size: 6301
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2452 3070 3167 1108
Size: 1344 618 97 4242
Fragments arranged by size:
4242 1344 618 97
PfoI T'CCnGG_A
Cuts at: 2151 2914 2151
Size: 763 5538
PmeI GTTT'AAAC
Cuts at: 3605 3605
Size: 6301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4370 4402 5883 5915 4370
Size: 32 1481 32 4756
Fragments arranged by size:
4756 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2402 2402
Size: 6301
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6301
PsiI TTA'TAA
Cuts at: 3871 3871
Size: 6301
PspOMI G'GGCC_C
Cuts at: 2586 2980 2586
Size: 394 5907
PstI C_TGCA'G
Cuts at: 830 2273 3653 830
Size: 1443 1380 3478
Fragments arranged by size:
3478 1443 1380
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6301
PvuII CAG'CTG
Cuts at: 2130 2757 2130
Size: 627 5674
SacI G_AGCT'C
Cuts at: 2768 2768
Size: 6301
SalI G'TCGA_C
Cuts at: 1936 3643 1936
Size: 1707 4594
SanDI GG'GwC_CC
Cuts at: 2402 2402
Size: 6301
SbfI CC_TGCA'GG
Cuts at: 3653 3653
Size: 6301
ScaI AGT'ACT
Cuts at: 4549 6261 4549
Size: 1712 4589
SexAI A'CCwGG_T
Cuts at: 2091 2091
Size: 6301
SfcI C'TryA_G
Cuts at: 826 1045 2269 3649 4784 5465 5656 826
Size: 219 1224 1380 1135 681 191 1471
Fragments arranged by size:
1471 1380 1224 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3206 3206
Size: 6301
SfoI GGC'GCC
Cuts at: 3264 3264
Size: 6301
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6301
SmaI CCC'GGG
Cuts at: 1872 2408 3103 3628 1872
Size: 536 695 525 4545
Fragments arranged by size:
4545 695 536 525
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2288 4405 5306 5568 820
Size: 197 926 345 2117 901 262 1553
Fragments arranged by size:
2117 1553 926 901 345 262 197
SnaBI TAC'GTA
Cuts at: 493 4092 493
Size: 3599 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6301
SphI G_CATG'C
Cuts at: 1132 3100 3659 1132
Size: 1968 559 3774
Fragments arranged by size:
3774 1968 559
SspI AAT'ATT
Cuts at: 5 52 4225 5
Size: 47 4173 2081
Fragments arranged by size:
4173 2081 47
StuI AGG'CCT
Cuts at: 1517 2634 2666 1517
Size: 1117 32 5152
Fragments arranged by size:
5152 1117 32
StyI C'CwwG_G
Cuts at: 513 1065 2004 2243 2553 2658 2685 2995 3138
Size: 552 939 239 310 105 27 310 143
Cuts at: 3138 3740 513
Size: 602 3074
Fragments arranged by size:
3074 939 602 552 310 310 239 143
105 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2439 3008 3009 3058 3062 3283 4328
Size: 1255 859 569 1 49 4 221 1045
Cuts at: 4328 4345 4498 4683 5128 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1045 859 569 445 221 185
153 49 17 4 1
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4547 6259 96
Size: 274 80 33 51 1443 2570 1712 138
Fragments arranged by size:
2570 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6261 6293 6261
Size: 32 6269
Tth111I GACn'n_nGTC
Cuts at: 1316 3293 1316
Size: 1977 4324
XbaI T'CTAG_A
Cuts at: 3637 3637
Size: 6301
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6301
XmaI C'CCGG_G
Cuts at: 1870 2406 3101 3626 1870
Size: 536 695 525 4545
Fragments arranged by size:
4545 695 536 525
XmnI GAAnn'nnTTC
Cuts at: 4430 4430
Size: 6301
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3526 4109 276
Size: 53 83 186 936 1992 583 2468
Fragments arranged by size:
2468 1992 936 583 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I EcoRV FalI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SapI SrfI
SwaI XcmI