Restriction map for
pFN21AB9741
(Circular) (Six-base) MAPSORT of: pFN21AB9741.seq Check: 732 from: 1 to: 6256
pFN21AB9741 6256 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2169 2169
Size: 6256
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4066 278
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2286 3599 1937
Size: 349 1313 4594
Fragments arranged by size:
4594 1313 349
Acc65I G'GTAC_C
Cuts at: 2549 3577 2549
Size: 1028 5228
AclI AA'CG_TT
Cuts at: 1563 4383 4756 1563
Size: 2820 373 3063
Fragments arranged by size:
3063 2820 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3100 4319 5703 5971 1632
Size: 1468 1219 1384 268 1917
Fragments arranged by size:
1917 1468 1384 1219 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6059
AflIII A'CryG_T
Cuts at: 1203 5155 6132 1203
Size: 3952 977 1327
Fragments arranged by size:
3952 1327 977
AgeI A'CCGG_T
Cuts at: 5087 5087
Size: 6256
AhdI GACnn_n'nnGTC
Cuts at: 2389 4985 2389
Size: 2596 3660
AleI CACnn'nnGTG
Cuts at: 1166 2734 2940 3155 1166
Size: 1568 206 215 4267
Fragments arranged by size:
4267 1568 215 206
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2201 2958 1184
Size: 1017 757 4482
Fragments arranged by size:
4482 1017 757
AlwNI CAG_nnn'CTG
Cuts at: 2419 2608 3339 3999 5571 2419
Size: 189 731 660 1572 3104
Fragments arranged by size:
3104 1572 731 660 189
ApaI G_GGCC'C
Cuts at: 2358 2358
Size: 6256
ApaLI G'TGCA_C
Cuts at: 2727 4313 5469 2727
Size: 1586 1156 3514
Fragments arranged by size:
3514 1586 1156
ApoI r'AATT_y
Cuts at: 1493 2422 2450 3107 3565 3791 3943 6052 1493
Size: 929 28 657 458 226 152 2109 1697
Fragments arranged by size:
2109 1697 929 657 458 226 152 28
AseI AT'TA_AT
Cuts at: 160 1032 4810 160
Size: 872 3778 1606
Fragments arranged by size:
3778 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
AvaI C'yCGr_G
Cuts at: 1870 1943 2119 2293 3470 3581 1870
Size: 73 176 174 1177 111 4545
Fragments arranged by size:
4545 1177 176 174 111 73
BamHI G'GATC_C
Cuts at: 2214 3586 3953 2214
Size: 1372 367 4517
Fragments arranged by size:
4517 1372 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2181 2549 2981 3092 3157
Size: 325 217 627 394 368 432 111 65
Cuts at: 3157 3310 3577 5032 618
Size: 153 267 1455 1842
Fragments arranged by size:
1842 1455 627 432 394 368 325 267
217 153 111 65
BanII G_rGCy'C
Cuts at: 1391 2358 2922 3058 3216 3243 3290 3399 3477
Size: 967 564 136 158 27 47 109 78
Cuts at: 3477 3575 1391
Size: 98 4072
Fragments arranged by size:
4072 967 564 158 136 109 98 78
47 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5285
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4447 4481 1903
Size: 34 2510 34 3678
Fragments arranged by size:
3678 2510 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4148 5358 4148
Size: 1210 5046
BclI T'GATC_A
Cuts at: 1548 2871 1548
Size: 1323 4933
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2121 2231 2720
Size: 107 122 71 631 605 449 110 489
Cuts at: 2720 4867 136
Size: 2147 1525
Fragments arranged by size:
2147 1525 631 605 489 449 122 110
107 71
BglII A'GATC_T
Cuts at: 6251 6251
Size: 6256
BlpI GC'TnA_GC
Cuts at: 3182 3673 3182
Size: 491 5765
Bme1580I G_kGCm'C
Cuts at: 1790 2126 2358 2731 2772 4317 5473 1790
Size: 336 232 373 41 1545 1156 2573
Fragments arranged by size:
2573 1545 1156 373 336 232 41
BmgBI CAC'GTC
Cuts at: 1318 3199 3262 3319 1318
Size: 1881 63 57 4255
Fragments arranged by size:
4255 1881 63 57
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3987 4945 442
Size: 416 53 83 399 24 2570 958 1753
Fragments arranged by size:
2570 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6256
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2941 2941
Size: 6256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2326 2428 3161 3230 3521 4916 1239
Size: 1087 102 733 69 291 1395 2579
Fragments arranged by size:
2579 1395 1087 733 291 102 69
Bpu10I CC'TnA_GC
Cuts at: 2060 2060
Size: 6256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3227 4381 5246 5544 3227
Size: 1154 865 298 3939
Fragments arranged by size:
3939 1154 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2653 2686 4919 882
Size: 617 1154 33 2233 2219
Fragments arranged by size:
2233 2219 1154 617 33
BsaAI yAC'GTr
Cuts at: 493 1204 4047 493
Size: 711 2843 2702
Fragments arranged by size:
2843 2702 711
BsaBI GATnn'nnATC
Cuts at: 6250 6250
Size: 6256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3465 3486 4063 4445
Size: 53 83 186 936 1932 21 577 382
Cuts at: 4445 5080 275
Size: 635 1451
Fragments arranged by size:
1932 1451 936 635 577 382 186 83
53 21
BsaWI w'CCGG_w
Cuts at: 3154 4689 5087 5361 5508 5919 3154
Size: 1535 398 274 147 411 3491
Fragments arranged by size:
3491 1535 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2848 2878 1178
Size: 30 165 30 1445 30 4556
Fragments arranged by size:
4556 1445 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6256
BseYI C'CCAG_C
Cuts at: 2545 2928 5459 5955 2545
Size: 383 2531 496 2846
Fragments arranged by size:
2846 2531 496 383
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2824 2849 2918 1163
Size: 573 1088 25 69 4501
Fragments arranged by size:
4501 1088 573 69 25
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3452 3706 4467 5495 1540
Size: 275 186 1451 254 761 1028 2301
Fragments arranged by size:
2301 1451 1028 761 275 254 186
BsiHKAI G_wGCw'C
Cuts at: 2151 2731 3216 4317 4402 5473 2151
Size: 580 485 1101 85 1071 2934
Fragments arranged by size:
2934 1101 1071 580 485 85
BsmI GAATG_Cn'
Cuts at: 2945 3767 3860 2945
Size: 822 93 5341
Fragments arranged by size:
5341 822 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2402 2402
Size: 6256
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 2151 2358 2731 2772 2922 3058
Size: 399 336 25 207 373 41 150 136
Cuts at: 3058 3216 3243 3290 3399 3477 3575 4317 4402
Size: 158 27 47 109 78 98 742 85
Cuts at: 4402 5473 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 373 336 207 158
150 136 109 98 85 78 47 41
27 25
BspEI T'CCGG_A
Cuts at: 5919 5919
Size: 6256
BspHI T'CATG_A
Cuts at: 4145 5875 4145
Size: 1730 4526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2169 3611 844
Size: 1325 1442 3489
Fragments arranged by size:
3489 1442 1325
BsrBI CCG'CTC
Cuts at: 1394 1823 3186 4143 1394
Size: 429 1363 957 3507
Fragments arranged by size:
3507 1363 957 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4751 4925 65
Size: 1183 3503 174 1396
Fragments arranged by size:
3503 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2849 3145 4900 5087 1700
Size: 110 1039 296 1755 187 2869
Fragments arranged by size:
2869 1755 1039 296 187 110
BsrGI T'GTAC_A
Cuts at: 96 2472 3279 96
Size: 2376 807 3073
Fragments arranged by size:
3073 2376 807
BssHII G'CGCG_C
Cuts at: 1925 3352 3385 1925
Size: 1427 33 4796
Fragments arranged by size:
4796 1427 33
BssSI C'ACGA_G
Cuts at: 4316 5328 4316
Size: 1012 5244
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6256
BstBI TT'CG_AA
Cuts at: 5060 5060
Size: 6256
BstEII G'GTnAC_C
Cuts at: 1184 2845 3455 1184
Size: 1661 610 3985
Fragments arranged by size:
3985 1661 610
BstXI CCAn_nnnn'nTGG
Cuts at: 3379 3379
Size: 6256
BstYI r'GATC_y
Cuts at: 1972 2214 3586 3953 4338 4355 5807 5893 5905
Size: 242 1372 367 385 17 1452 86 12
Cuts at: 5905 6251 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1372 385 367 346 242 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2358 3217 3290 513
Size: 552 939 354 859 73 3479
Fragments arranged by size:
3479 939 859 552 354 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5517
Fragments arranged by size:
5517 683 56
BtsI GCAGTG_nn'
Cuts at: 3772 4566 4586 3772
Size: 794 20 5442
Fragments arranged by size:
5442 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6221
DraI TTT'AAA
Cuts at: 3560 3560
Size: 6256
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5263 809
Size: 507 3947 1802
Fragments arranged by size:
3947 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2721 3201 3449
Size: 54 1425 50 275 18 891 480 248
Cuts at: 3449 3703 4592 6221 8
Size: 254 889 1629 43
Fragments arranged by size:
1629 1425 891 889 480 275 254 248
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3449 3703 1537
Size: 275 1637 254 4090
Fragments arranged by size:
4090 1637 275 254
EarI CTCTTCn'nnn_
Cuts at: 1353 2660 3404 3548 4186 1353
Size: 1307 744 144 638 3423
Fragments arranged by size:
3423 1307 744 638 144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2125 4843 5217 5363 387
Size: 1064 138 536 2718 374 146 1280
Fragments arranged by size:
2718 1280 1064 536 374 146 138
EcoICRI GAG'CTC
Cuts at: 3214 3214
Size: 6256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2326 2428 3100 3161 3230 3521 4319
Size: 393 694 102 672 61 69 291 798
Cuts at: 4319 4916 5703 5971 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 798 787 694 672 597 393 291
268 102 69 61
EcoNI CCTnn'n_nnAGG
Cuts at: 2570 2570
Size: 6256
EcoO109I rG'GnC_Cy
Cuts at: 2297 2354 2712 2297
Size: 57 358 5841
Fragments arranged by size:
5841 358 57
EcoRI G'AATT_C
Cuts at: 2450 3565 3943 2450
Size: 1115 378 4763
Fragments arranged by size:
4763 1115 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2281 2281
Size: 6256
FspI TGC'GCA
Cuts at: 2177 4762 2177
Size: 2585 3671
HaeII r_GCGC'y
Cuts at: 1678 2403 3346 5403 1678
Size: 725 943 2057 2531
Fragments arranged by size:
2531 2057 943 725
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2375 2407 2756 2788 3496
Size: 32 135 32 803 32 349 32 708
Cuts at: 3496 3528 4897 4929 4971 5003 1373
Size: 32 1369 32 42 32 2626
Fragments arranged by size:
2626 1369 803 708 349 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3600 3846 669
Size: 988 281 1662 246 3079
Fragments arranged by size:
3079 1662 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6256
HpaI GTT'AAC
Cuts at: 3846 3846
Size: 6256
KpnI G_GTAC'C
Cuts at: 2553 3581 2553
Size: 1028 5228
MfeI C'AATT_G
Cuts at: 3855 3855
Size: 6256
MluI A'CGCG_T
Cuts at: 6132 6132
Size: 6256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5370 5554 1305
Size: 4065 184 2007
Fragments arranged by size:
4065 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2723 3203 10
Size: 54 1425 343 891 480 3063
Fragments arranged by size:
3063 1425 891 480 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2734 2940 3155 4214 4573 4732 518
Size: 648 1568 206 215 1059 359 159 2042
Fragments arranged by size:
2042 1568 1059 648 359 215 206 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2932 3292 3672 4349 5497 5742 1606
Size: 483 843 360 380 677 1148 245 2120
Fragments arranged by size:
2120 1148 843 677 483 380 360 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4765
Fragments arranged by size:
4765 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6256
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6256
NotI GC'GGCC_GC
Cuts at: 3703 3703
Size: 6256
NspI r_CATG'y
Cuts at: 1132 1229 2225 3614 5159 1132
Size: 97 996 1389 1545 2229
Fragments arranged by size:
2229 1545 1389 996 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6256
PciI A'CATG_T
Cuts at: 5155 5155
Size: 6256
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6256
PfoI T'CCnGG_A
Cuts at: 3498 3498
Size: 6256
PmeI GTTT'AAAC
Cuts at: 3560 3560
Size: 6256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2246 2278 4325 4357 5838 5870 2246
Size: 32 2047 32 1481 32 2632
Fragments arranged by size:
2632 2047 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6256
PsiI TTA'TAA
Cuts at: 3826 3826
Size: 6256
PspOMI G'GGCC_C
Cuts at: 2354 2354
Size: 6256
PstI C_TGCA'G
Cuts at: 830 2070 2094 2190 3335 3608 830
Size: 1240 24 96 1145 273 3478
Fragments arranged by size:
3478 1240 1145 273 96 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6256
PvuII CAG'CTG
Cuts at: 2089 2932 2089
Size: 843 5413
SacI G_AGCT'C
Cuts at: 3216 3216
Size: 6256
SacII CC_GC'GG
Cuts at: 3293 3293
Size: 6256
SalI G'TCGA_C
Cuts at: 1936 3598 1936
Size: 1662 4594
SapI GCTCTTCn'nnn_
Cuts at: 3404 3404
Size: 6256
SbfI CC_TGCA'GG
Cuts at: 2070 3608 2070
Size: 1538 4718
ScaI AGT'ACT
Cuts at: 4504 6216 4504
Size: 1712 4544
SexAI A'CCwGG_T
Cuts at: 2132 2132
Size: 6256
SfcI C'TryA_G
Cuts at: 826 1045 2066 2090 2186 3331 3604 4739 5420
Size: 219 1021 24 96 1145 273 1135 681
Cuts at: 5420 5611 826
Size: 191 1471
Fragments arranged by size:
1471 1145 1135 1021 681 273 219 191
96 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 2720 2720
Size: 6256
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6256
SmaI CCC'GGG
Cuts at: 1872 2121 3583 1872
Size: 249 1462 4545
Fragments arranged by size:
4545 1462 249
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3242 4360 5261 5523 820
Size: 197 926 1299 1118 901 262 1553
Fragments arranged by size:
1553 1299 1118 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4047 493
Size: 3554 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6256
SphI G_CATG'C
Cuts at: 1132 2225 3614 1132
Size: 1093 1389 3774
Fragments arranged by size:
3774 1389 1093
SspI AAT'ATT
Cuts at: 5 52 4180 5
Size: 47 4128 2081
Fragments arranged by size:
4128 2081 47
StuI AGG'CCT
Cuts at: 1517 3026 1517
Size: 1509 4747
StyI C'CwwG_G
Cuts at: 513 1065 2004 2226 2779 3039 3695 513
Size: 552 939 222 553 260 656 3074
Fragments arranged by size:
3074 939 656 553 552 260 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4283 4300 4453 4638 5083 325
Size: 1255 2703 17 153 185 445 1498
Fragments arranged by size:
2703 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2472 3279 4502
Size: 274 80 33 51 1443 495 807 1223
Cuts at: 4502 6214 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1223 807 495 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2848 2880 6216 6248 2848
Size: 32 3336 32 2856
Fragments arranged by size:
3336 2856 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2134 1316
Size: 818 5438
XbaI T'CTAG_A
Cuts at: 3592 3592
Size: 6256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2762 3007 2762
Size: 245 6011
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6256
XmaI C'CCGG_G
Cuts at: 1870 2119 3581 1870
Size: 249 1462 4545
Fragments arranged by size:
4545 1462 249
XmnI GAAnn'nnTTC
Cuts at: 2261 4385 2261
Size: 2124 4132
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4064 276
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SanDI SfoI SrfI SwaI