Restriction map for
pFN21AB9739
(Circular) (Six-base) MAPSORT of: pFN21AB9739.seq Check: 5178 from: 1 to: 6226
pFN21AB9739 6226 bp
With 182 enzymes: SgfI *
March 7, 2011 13:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3038 3038
Size: 6226
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2897 4036 278
Size: 53 83 186 936 1361 1139 2468
Fragments arranged by size:
2468 1361 1139 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3569 1937
Size: 1632 4594
Acc65I G'GTAC_C
Cuts at: 2404 3547 2404
Size: 1143 5083
AclI AA'CG_TT
Cuts at: 1563 4353 4726 1563
Size: 2790 373 3063
Fragments arranged by size:
3063 2790 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2026 3039 4289 5673 5941 1632
Size: 394 1013 1250 1384 268 1917
Fragments arranged by size:
1917 1384 1250 1013 394 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6029
AflIII A'CryG_T
Cuts at: 1203 5125 6102 1203
Size: 3922 977 1327
Fragments arranged by size:
3922 1327 977
AgeI A'CCGG_T
Cuts at: 3351 5057 3351
Size: 1706 4520
AhdI GACnn_n'nnGTC
Cuts at: 4955 4955
Size: 6226
AleI CACnn'nnGTG
Cuts at: 1166 2267 2963 1166
Size: 1101 696 4429
Fragments arranged by size:
4429 1101 696
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6226
AlwNI CAG_nnn'CTG
Cuts at: 3969 5541 3969
Size: 1572 4654
ApaI G_GGCC'C
Cuts at: 3068 3068
Size: 6226
ApaLI G'TGCA_C
Cuts at: 4283 5439 4283
Size: 1156 5070
ApoI r'AATT_y
Cuts at: 1493 3418 3484 3535 3761 3913 6022 1493
Size: 1925 66 51 226 152 2109 1697
Fragments arranged by size:
2109 1925 1697 226 152 66 51
AseI AT'TA_AT
Cuts at: 160 1032 4780 160
Size: 872 3748 1606
Fragments arranged by size:
3748 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6226
AvaI C'yCGr_G
Cuts at: 1870 1943 2634 3551 1870
Size: 73 691 917 4545
Fragments arranged by size:
4545 917 691 73
AvrII C'CTAG_G
Cuts at: 2969 2969
Size: 6226
BamHI G'GATC_C
Cuts at: 3317 3556 3923 3317
Size: 239 367 5620
Fragments arranged by size:
5620 367 239
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2404 3337 3547 5002 618
Size: 325 217 627 617 933 210 1455 1842
Fragments arranged by size:
1842 1455 933 627 617 325 217 210
BanII G_rGCy'C
Cuts at: 1391 2093 2466 3068 3545 1391
Size: 702 373 602 477 4072
Fragments arranged by size:
4072 702 602 477 373
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2563 2832 2919 928
Size: 971 664 269 87 4235
Fragments arranged by size:
4235 971 664 269 87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3172 3206 4417 4451 1903
Size: 34 50 34 1151 34 1211 34 3678
Fragments arranged by size:
3678 1211 1151 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4118 5328 4118
Size: 1210 5016
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6226
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4837 136
Size: 107 122 71 631 605 3165 1525
Fragments arranged by size:
3165 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6221 6221
Size: 6226
BlpI GC'TnA_GC
Cuts at: 2460 3643 2460
Size: 1183 5043
Bme1580I G_kGCm'C
Cuts at: 1790 2235 2942 3068 4287 5443 1790
Size: 445 707 126 1219 1156 2573
Fragments arranged by size:
2573 1219 1156 707 445 126
BmgBI CAC'GTC
Cuts at: 1318 2086 1318
Size: 768 5458
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2721 2952 3071
Size: 416 53 83 399 24 1304 231 119
Cuts at: 3071 3957 4915 442
Size: 886 958 1753
Fragments arranged by size:
1753 1304 958 886 416 399 231 119
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2038 4886 1239
Size: 799 2848 2579
Fragments arranged by size:
2848 2579 799
Bpu10I CC'TnA_GC
Cuts at: 2882 2882
Size: 6226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4351 5216 5514 4351
Size: 865 298 5063
Fragments arranged by size:
5063 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3342 3360 4889 882
Size: 617 1843 18 1529 2219
Fragments arranged by size:
2219 1843 1529 617 18
BsaAI yAC'GTr
Cuts at: 493 1204 4017 493
Size: 711 2813 2702
Fragments arranged by size:
2813 2702 711
BsaBI GATnn'nnATC
Cuts at: 6220 6220
Size: 6226
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2894 4033 4415 5050
Size: 53 83 186 936 1361 1139 382 635
Cuts at: 5050 275
Size: 1451
Fragments arranged by size:
1451 1361 1139 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2466 3351 4659 5057 5331 5478 5889 2466
Size: 885 1308 398 274 147 411 2803
Fragments arranged by size:
2803 1308 885 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2566 2596 1178
Size: 30 165 30 1163 30 4808
Fragments arranged by size:
4808 1163 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6226
BseYI C'CCAG_C
Cuts at: 5429 5925 5429
Size: 496 5730
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2763 1163
Size: 573 1027 4626
Fragments arranged by size:
4626 1027 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2246 2295 3676 4437 5465 1540
Size: 275 186 245 49 1381 761 1028 2301
Fragments arranged by size:
2301 1381 1028 761 275 245 186 49
BsiHKAI G_wGCw'C
Cuts at: 2030 2333 2466 4287 4372 5443 2030
Size: 303 133 1821 85 1071 2813
Fragments arranged by size:
2813 1821 1071 303 133 85
BsmI GAATG_Cn'
Cuts at: 2476 2715 3141 3372 3737 3830 2476
Size: 239 426 231 365 93 4872
Fragments arranged by size:
4872 426 365 239 231 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2093 2235 2333 2466 2942 3068
Size: 399 240 63 142 98 133 476 126
Cuts at: 3068 3545 4287 4372 5443 1391
Size: 477 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 477 476 399 240 142
133 126 98 85 63
BspEI T'CCGG_A
Cuts at: 2466 5889 2466
Size: 3423 2803
BspHI T'CATG_A
Cuts at: 4115 5845 4115
Size: 1730 4496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3038 3234 3581 844
Size: 2194 196 347 3489
Fragments arranged by size:
3489 2194 347 196
BsrBI CCG'CTC
Cuts at: 1394 1823 4113 1394
Size: 429 2290 3507
Fragments arranged by size:
3507 2290 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4721 4895 65
Size: 1183 3473 174 1396
Fragments arranged by size:
3473 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2034 3351 4870 5057 1700
Size: 110 224 1317 1519 187 2869
Fragments arranged by size:
2869 1519 1317 224 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6226
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6226
BssSI C'ACGA_G
Cuts at: 4286 5298 4286
Size: 1012 5214
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6226
BstBI TT'CG_AA
Cuts at: 5030 5030
Size: 6226
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6226
BstXI CCAn_nnnn'nTGG
Cuts at: 3437 3437
Size: 6226
BstYI r'GATC_y
Cuts at: 1972 2020 3317 3556 3923 4308 4325 5777 5863
Size: 48 1297 239 367 385 17 1452 86
Cuts at: 5863 5875 6221 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1297 385 367 346 239 86
48 17 12
Bsu36I CC'TnA_GG
Cuts at: 3178 3178
Size: 6226
BtgI C'CryG_G
Cuts at: 513 1065 2265 2373 2759 2849 3333 513
Size: 552 1200 108 386 90 484 3406
Fragments arranged by size:
3406 1200 552 484 386 108 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2263 487
Size: 683 56 1037 4450
Fragments arranged by size:
4450 1037 683 56
BtsI GCAGTG_nn'
Cuts at: 2235 2991 3742 4536 4556 2235
Size: 756 751 794 20 3905
Fragments arranged by size:
3905 794 756 751 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6226
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6191
DraI TTT'AAA
Cuts at: 3530 3530
Size: 6226
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2899 5233 809
Size: 507 1583 2334 1802
Fragments arranged by size:
2334 1802 1583 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2243 2292 3673
Size: 54 1425 50 275 18 413 49 1381
Cuts at: 3673 4562 6191 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1381 889 413 275 54 50
49 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2243 2292 3673 1537
Size: 275 431 49 1381 4090
Fragments arranged by size:
4090 1381 431 275 49
EarI CTCTTCn'nnn_
Cuts at: 1353 3032 3253 4156 1353
Size: 1679 221 903 3423
Fragments arranged by size:
3423 1679 903 221
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3190 3363 4813 5187 5333 387
Size: 1064 138 1601 173 1450 374 146 1280
Fragments arranged by size:
1601 1450 1280 1064 374 173 146 138
EcoICRI GAG'CTC
Cuts at: 2464 2464
Size: 6226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2026 2038 3039 4289 4886 5673 5941
Size: 393 394 12 1001 1250 597 787 268
Cuts at: 5941 1239
Size: 1524
Fragments arranged by size:
1524 1250 1001 787 597 394 393 268
12
EcoNI CCTnn'n_nnAGG
Cuts at: 2399 2399
Size: 6226
EcoO109I rG'GnC_Cy
Cuts at: 3065 3065
Size: 6226
EcoRI G'AATT_C
Cuts at: 3484 3535 3913 3484
Size: 51 378 5797
Fragments arranged by size:
5797 378 51
FspI TGC'GCA
Cuts at: 2106 4732 2106
Size: 2626 3600
HaeII r_GCGC'y
Cuts at: 1678 2041 2356 5373 1678
Size: 363 315 3017 2531
Fragments arranged by size:
3017 2531 363 315
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2727 2759 4867 4899 4941
Size: 32 135 32 1155 32 2108 32 42
Cuts at: 4941 4973 1373
Size: 32 2626
Fragments arranged by size:
2626 2108 1155 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3445 3570 3816 669
Size: 988 281 1507 125 246 3079
Fragments arranged by size:
3079 1507 988 281 246 125
HindIII A'AGCT_T
Cuts at: 748 2660 748
Size: 1912 4314
HpaI GTT'AAC
Cuts at: 3816 3816
Size: 6226
KpnI G_GTAC'C
Cuts at: 2408 3551 2408
Size: 1143 5083
MfeI C'AATT_G
Cuts at: 3825 3825
Size: 6226
MluI A'CGCG_T
Cuts at: 6102 6102
Size: 6226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3150 5340 5524 1305
Size: 1845 2190 184 2007
Fragments arranged by size:
2190 2007 1845 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4404
Fragments arranged by size:
4404 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2267 2963 4184 4543 4702 518
Size: 648 1101 696 1221 359 159 2042
Fragments arranged by size:
2042 1221 1101 696 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2225 2923 3642 4319 5467 5712 1606
Size: 619 698 719 677 1148 245 2120
Fragments arranged by size:
2120 1148 719 698 677 619 245
NaeI GCC'GGC
Cuts at: 2036 2036
Size: 6226
NcoI C'CATG_G
Cuts at: 513 1065 2265 3333 513
Size: 552 1200 1068 3406
Fragments arranged by size:
3406 1200 1068 552
NdeI CA'TA_TG
Cuts at: 387 3523 387
Size: 3136 3090
NgoMIV G'CCGG_C
Cuts at: 2034 2034
Size: 6226
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6226
NotI GC'GGCC_GC
Cuts at: 2243 3673 2243
Size: 1430 4796
NspI r_CATG'y
Cuts at: 1132 1229 3187 3584 5129 1132
Size: 97 1958 397 1545 2229
Fragments arranged by size:
2229 1958 1545 397 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6226
PciI A'CATG_T
Cuts at: 5125 5125
Size: 6226
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6226
PmeI GTTT'AAAC
Cuts at: 3530 3530
Size: 6226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4295 4327 5808 5840 4295
Size: 32 1481 32 4681
Fragments arranged by size:
4681 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6226
PsiI TTA'TAA
Cuts at: 3796 3796
Size: 6226
PspOMI G'GGCC_C
Cuts at: 3064 3064
Size: 6226
PstI C_TGCA'G
Cuts at: 830 2067 2230 3578 830
Size: 1237 163 1348 3478
Fragments arranged by size:
3478 1348 1237 163
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6226
PvuII CAG'CTG
Cuts at: 2225 2225
Size: 6226
SacI G_AGCT'C
Cuts at: 2466 2466
Size: 6226
SalI G'TCGA_C
Cuts at: 1936 3568 1936
Size: 1632 4594
SapI GCTCTTCn'nnn_
Cuts at: 3032 3253 3032
Size: 221 6005
SbfI CC_TGCA'GG
Cuts at: 3578 3578
Size: 6226
ScaI AGT'ACT
Cuts at: 4474 6186 4474
Size: 1712 4514
SfcI C'TryA_G
Cuts at: 826 1045 2063 2226 3574 4709 5390 5581 826
Size: 219 1018 163 1348 1135 681 191 1471
Fragments arranged by size:
1471 1348 1135 1018 681 219 191 163
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6226
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6226
SmaI CCC'GGG
Cuts at: 1872 3553 1872
Size: 1681 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4330 5231 5493 820
Size: 197 926 2387 901 262 1553
Fragments arranged by size:
2387 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4017 493
Size: 3524 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6226
SphI G_CATG'C
Cuts at: 1132 3584 1132
Size: 2452 3774
SspI AAT'ATT
Cuts at: 5 52 4150 5
Size: 47 4098 2081
Fragments arranged by size:
4098 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6226
StyI C'CwwG_G
Cuts at: 513 1065 2265 2969 3333 3665 513
Size: 552 1200 704 364 332 3074
Fragments arranged by size:
3074 1200 704 552 364 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4253 4270 4423 4608 5053 325
Size: 1255 2673 17 153 185 445 1498
Fragments arranged by size:
2673 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4472 6184 96
Size: 274 80 33 51 1443 2495 1712 138
Fragments arranged by size:
2495 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2566 2598 2932 2964 3452 3484 6186 6218 2566
Size: 32 334 32 488 32 2702 32 2574
Fragments arranged by size:
2702 2574 488 334 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2808 1316
Size: 1492 4734
XbaI T'CTAG_A
Cuts at: 3562 3562
Size: 6226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3260 3260
Size: 6226
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6226
XmaI C'CCGG_G
Cuts at: 1870 3551 1870
Size: 1681 4545
XmnI GAAnn'nnTTC
Cuts at: 4355 4355
Size: 6226
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2895 4034 276
Size: 53 83 186 936 1361 1139 2468
Fragments arranged by size:
2468 1361 1139 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI