Restriction map for
pFN21AB9738
(Circular) (Six-base) MAPSORT of: pFN21AB9738.seq Check: 7705 from: 1 to: 6214
pFN21AB9738 6214 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2199 2199
Size: 6214
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4024 278
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3557 1937
Size: 1620 4594
Acc65I G'GTAC_C
Cuts at: 3535 3535
Size: 6214
AclI AA'CG_TT
Cuts at: 1563 4341 4714 1563
Size: 2778 373 3063
Fragments arranged by size:
3063 2778 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3208 4277 5661 5929 1632
Size: 1576 1069 1384 268 1917
Fragments arranged by size:
1917 1576 1384 1069 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6017
AflIII A'CryG_T
Cuts at: 1203 2035 2309 5113 6090 1203
Size: 832 274 2804 977 1327
Fragments arranged by size:
2804 1327 977 832 274
AgeI A'CCGG_T
Cuts at: 5045 5045
Size: 6214
AhdI GACnn_n'nnGTC
Cuts at: 4943 4943
Size: 6214
AleI CACnn'nnGTG
Cuts at: 1166 2843 3100 1166
Size: 1677 257 4280
Fragments arranged by size:
4280 1677 257
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2861 2921 1184
Size: 1677 60 4477
Fragments arranged by size:
4477 1677 60
AlwNI CAG_nnn'CTG
Cuts at: 2079 2101 3316 3444 3957 5529 2079
Size: 22 1215 128 513 1572 2764
Fragments arranged by size:
2764 1572 1215 513 128 22
ApaI G_GGCC'C
Cuts at: 2466 2748 3109 2466
Size: 282 361 5571
Fragments arranged by size:
5571 361 282
ApaLI G'TGCA_C
Cuts at: 3383 4271 5427 3383
Size: 888 1156 4170
Fragments arranged by size:
4170 1156 888
ApoI r'AATT_y
Cuts at: 1493 3251 3523 3749 3901 6010 1493
Size: 1758 272 226 152 2109 1697
Fragments arranged by size:
2109 1758 1697 272 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4768 160
Size: 872 3736 1606
Fragments arranged by size:
3736 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
AvaI C'yCGr_G
Cuts at: 1870 1943 2246 2338 2741 2926 3539 1870
Size: 73 303 92 403 185 613 4545
Fragments arranged by size:
4545 613 403 303 185 92 73
BamHI G'GATC_C
Cuts at: 3544 3911 3544
Size: 367 5847
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3535 4990 618
Size: 325 217 627 1748 1455 1842
Fragments arranged by size:
1842 1748 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2466 2694 2748 2856 3015 3109 3533 1391
Size: 1075 228 54 108 159 94 424 4072
Fragments arranged by size:
4072 1075 424 228 159 108 94 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3223 3408 928
Size: 971 1324 185 3734
Fragments arranged by size:
3734 1324 971 185
BbvCI CC'TCA_GC
Cuts at: 3437 3437
Size: 6214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4405 4439 1903
Size: 34 2468 34 3678
Fragments arranged by size:
3678 2468 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4106 5316 4106
Size: 1210 5004
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6214
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2470 4825 136
Size: 107 122 71 631 605 798 2355 1525
Fragments arranged by size:
2355 1525 798 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6209 6209
Size: 6214
BlpI GC'TnA_GC
Cuts at: 2375 3631 2375
Size: 1256 4958
Bme1580I G_kGCm'C
Cuts at: 1790 2133 2466 2748 3109 3262 3387 3398 4275
Size: 343 333 282 361 153 125 11 877
Cuts at: 4275 5431 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 877 361 343 333 282 153
125 11
BmgBI CAC'GTC
Cuts at: 1318 2875 1318
Size: 1557 4657
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2676 3145 3945
Size: 416 53 83 399 24 1259 469 800
Cuts at: 3945 4903 442
Size: 958 1753
Fragments arranged by size:
1753 1259 958 800 469 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6214
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2072 2072
Size: 6214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2540 2658 2700 3101 3521 4874 1239
Size: 1301 118 42 401 420 1353 2579
Fragments arranged by size:
2579 1353 1301 420 401 118 42
Bpu10I CC'TnA_GC
Cuts at: 2159 3437 2159
Size: 1278 4936
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4339 5204 5502 4339
Size: 865 298 5051
Fragments arranged by size:
5051 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4877 882
Size: 617 3378 2219
Fragments arranged by size:
3378 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3061 4005 493
Size: 711 1857 944 2702
Fragments arranged by size:
2702 1857 944 711
BsaBI GATnn'nnATC
Cuts at: 6208 6208
Size: 6214
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4021 4403 5038 275
Size: 53 83 186 936 2488 382 635 1451
Fragments arranged by size:
2488 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4647 5045 5319 5466 5877 4647
Size: 398 274 147 411 4984
Fragments arranged by size:
4984 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2617 2647 1178
Size: 30 165 30 1214 30 4745
Fragments arranged by size:
4745 1214 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6214
BseYI C'CCAG_C
Cuts at: 2527 2699 2735 2748 2855 2959 2982 2999 3178
Size: 172 36 13 107 104 23 17 179
Cuts at: 3178 3323 5417 5913 2527
Size: 145 2094 496 2828
Fragments arranged by size:
2828 2094 496 179 172 145 107 104
36 23 17 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2821 2917 1163
Size: 573 1085 96 4460
Fragments arranged by size:
4460 1085 573 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2352 3664 4425 5453 1540
Size: 275 186 351 1312 761 1028 2301
Fragments arranged by size:
2301 1312 1028 761 351 275 186
BsiHKAI G_wGCw'C
Cuts at: 3015 3387 4275 4360 5431 3015
Size: 372 888 85 1071 3798
Fragments arranged by size:
3798 1071 888 372 85
BsmI GAATG_Cn'
Cuts at: 2993 3725 3818 2993
Size: 732 93 5389
Fragments arranged by size:
5389 732 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2720 2720
Size: 6214
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2466 2694 2748 2856 3015 3109
Size: 399 343 333 228 54 108 159 94
Cuts at: 3109 3262 3387 3398 3533 4275 4360 5431 1391
Size: 153 125 11 135 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 343 333 228 159
153 135 125 108 94 85 54 11
BspEI T'CCGG_A
Cuts at: 5877 5877
Size: 6214
BspHI T'CATG_A
Cuts at: 4103 5833 4103
Size: 1730 4484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2199 2977 3569 844
Size: 1355 778 592 3489
Fragments arranged by size:
3489 1355 778 592
BsrBI CCG'CTC
Cuts at: 1394 1823 4101 1394
Size: 429 2278 3507
Fragments arranged by size:
3507 2278 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4709 4883 65
Size: 1183 3461 174 1396
Fragments arranged by size:
3461 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2270 4858 5045 1700
Size: 110 460 2588 187 2869
Fragments arranged by size:
2869 2588 460 187 110
BsrGI T'GTAC_A
Cuts at: 96 2886 96
Size: 2790 3424
BssHII G'CGCG_C
Cuts at: 1925 2147 2174 2266 1925
Size: 222 27 92 5873
Fragments arranged by size:
5873 222 92 27
BssSI C'ACGA_G
Cuts at: 4274 5286 4274
Size: 1012 5202
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2079 794
Size: 1285 4929
BstBI TT'CG_AA
Cuts at: 5018 5018
Size: 6214
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6214
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3102 3298 2011
Size: 1091 196 4927
Fragments arranged by size:
4927 1091 196
BstYI r'GATC_y
Cuts at: 1972 2614 3544 3911 4296 4313 5765 5851 5863
Size: 642 930 367 385 17 1452 86 12
Cuts at: 5863 6209 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 930 642 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2203 2754 2778 2841 3271 513
Size: 552 939 199 551 24 63 430 3456
Fragments arranged by size:
3456 939 552 551 430 199 63 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5475
Fragments arranged by size:
5475 683 56
BtsI GCAGTG_nn'
Cuts at: 2102 2297 2568 3730 4524 4544 2102
Size: 195 271 1162 794 20 3772
Fragments arranged by size:
3772 1162 794 271 195 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6214
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6179
DraI TTT'AAA
Cuts at: 3518 3518
Size: 6214
DraIII CAC_nnn'GTG
Cuts at: 2365 2459 2365
Size: 94 6120
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2963 3227 5221 809
Size: 507 1647 264 1994 1802
Fragments arranged by size:
1994 1802 1647 507 264
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2213 2349 3661
Size: 54 1425 50 275 18 383 136 1312
Cuts at: 3661 4550 6179 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1312 889 383 275 136 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2349 3661 1537
Size: 275 537 1312 4090
Fragments arranged by size:
4090 1312 537 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3335 4144 1353
Size: 1982 809 3423
Fragments arranged by size:
3423 1982 809
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3151 3162 4801 5175 5321 387
Size: 1064 138 1562 11 1639 374 146 1280
Fragments arranged by size:
1639 1562 1280 1064 374 146 138 11
EcoICRI GAG'CTC
Cuts at: 3013 3013
Size: 6214
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2540 2658 2700 3101 3208 3521 4277
Size: 393 908 118 42 401 107 313 756
Cuts at: 4277 4874 5661 5929 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 908 787 756 597 401 393 313
268 118 107 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2162 2555 2162
Size: 393 5821
EcoO109I rG'GnC_Cy
Cuts at: 2155 2344 2431 2463 2513 2545 2670 2745 3313
Size: 189 87 32 50 32 125 75 568
Cuts at: 3313 2155
Size: 5056
Fragments arranged by size:
5056 568 189 125 87 75 50 32
32
EcoRI G'AATT_C
Cuts at: 3251 3523 3901 3251
Size: 272 378 5564
Fragments arranged by size:
5564 378 272
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2672 2672
Size: 6214
FspI TGC'GCA
Cuts at: 2296 2303 4720 2296
Size: 7 2417 3790
Fragments arranged by size:
3790 2417 7
HaeII r_GCGC'y
Cuts at: 1678 2835 2870 3271 3459 5361 1678
Size: 1157 35 401 188 1902 2531
Fragments arranged by size:
2531 1902 1157 401 188 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3398 3430 4855 4887 4929
Size: 32 135 32 1826 32 1425 32 42
Cuts at: 4929 4961 1373
Size: 32 2626
Fragments arranged by size:
2626 1826 1425 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3558 3804 669
Size: 988 281 1620 246 3079
Fragments arranged by size:
3079 1620 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6214
HpaI GTT'AAC
Cuts at: 3804 3804
Size: 6214
KpnI G_GTAC'C
Cuts at: 3539 3539
Size: 6214
MfeI C'AATT_G
Cuts at: 3813 3813
Size: 6214
MluI A'CGCG_T
Cuts at: 2035 2309 6090 2035
Size: 274 3781 2159
Fragments arranged by size:
3781 2159 274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5328 5512 1305
Size: 4023 184 2007
Fragments arranged by size:
4023 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4392
Fragments arranged by size:
4392 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2490 2843 3100 4172 4531 4690 518
Size: 648 1324 353 257 1072 359 159 2042
Fragments arranged by size:
2042 1324 1072 648 359 353 257 159
MspA1I CmG'CkG
Cuts at: 1606 2076 2205 2292 2441 2531 2756 2780 2843
Size: 470 129 87 149 90 225 24 63
Cuts at: 2843 2963 3031 3100 3323 3630 4307 5455 5700
Size: 120 68 69 223 307 677 1148 245
Cuts at: 5700 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 470 307 245 225 223
149 129 120 90 87 69 68 63
24
NaeI GCC'GGC
Cuts at: 2272 2272
Size: 6214
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4723
Fragments arranged by size:
4723 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6214
NgoMIV G'CCGG_C
Cuts at: 2270 2270
Size: 6214
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6214
NotI GC'GGCC_GC
Cuts at: 3661 3661
Size: 6214
NspI r_CATG'y
Cuts at: 1132 1229 2495 3572 5117 1132
Size: 97 1266 1077 1545 2229
Fragments arranged by size:
2229 1545 1266 1077 97
PasI CC'CwG_GG
Cuts at: 1348 2468 2687 2861 3068 1348
Size: 1120 219 174 207 4494
Fragments arranged by size:
4494 1120 219 207 174
PciI A'CATG_T
Cuts at: 5113 5113
Size: 6214
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2459 3244 1108
Size: 1351 785 4078
Fragments arranged by size:
4078 1351 785
PfoI T'CCnGG_A
Cuts at: 2635 3015 2635
Size: 380 5834
PmeI GTTT'AAAC
Cuts at: 3518 3518
Size: 6214
PmlI CAC'GTG
Cuts at: 3061 3061
Size: 6214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4283 4315 5796 5828 4283
Size: 32 1481 32 4669
Fragments arranged by size:
4669 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2155 2344 2545 3313 2155
Size: 189 201 768 5056
Fragments arranged by size:
5056 768 201 189
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6214
PsiI TTA'TAA
Cuts at: 3784 3784
Size: 6214
PspOMI G'GGCC_C
Cuts at: 2462 2744 3105 2462
Size: 282 361 5571
Fragments arranged by size:
5571 361 282
PstI C_TGCA'G
Cuts at: 830 2097 2910 3566 830
Size: 1267 813 656 3478
Fragments arranged by size:
3478 1267 813 656
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6214
PvuII CAG'CTG
Cuts at: 2441 2531 2963 3031 3100 3323 2441
Size: 90 432 68 69 223 5332
Fragments arranged by size:
5332 432 223 90 69 68
SacI G_AGCT'C
Cuts at: 3015 3015
Size: 6214
SacII CC_GC'GG
Cuts at: 2206 2757 2781 2844 2206
Size: 551 24 63 5576
Fragments arranged by size:
5576 551 63 24
SalI G'TCGA_C
Cuts at: 1936 3556 1936
Size: 1620 4594
SanDI GG'GwC_CC
Cuts at: 2344 2344
Size: 6214
SapI GCTCTTCn'nnn_
Cuts at: 3335 3335
Size: 6214
SbfI CC_TGCA'GG
Cuts at: 3566 3566
Size: 6214
ScaI AGT'ACT
Cuts at: 4462 6174 4462
Size: 1712 4502
SfcI C'TryA_G
Cuts at: 826 1045 2093 2906 3562 4697 5378 5569 826
Size: 219 1048 813 656 1135 681 191 1471
Fragments arranged by size:
1471 1135 1048 813 681 656 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2470 2470
Size: 6214
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6214
SmaI CCC'GGG
Cuts at: 1872 2743 3541 1872
Size: 871 798 4545
Fragments arranged by size:
4545 871 798
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4318 5219 5481 820
Size: 197 926 2375 901 262 1553
Fragments arranged by size:
2375 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4005 493
Size: 3512 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6214
SphI G_CATG'C
Cuts at: 1132 3572 1132
Size: 2440 3774
SspI AAT'ATT
Cuts at: 5 52 4138 5
Size: 47 4086 2081
Fragments arranged by size:
4086 2081 47
StuI AGG'CCT
Cuts at: 1517 2189 1517
Size: 672 5542
StyI C'CwwG_G
Cuts at: 513 1065 2004 2010 2059 2628 3333 3653 513
Size: 552 939 6 49 569 705 320 3074
Fragments arranged by size:
3074 939 705 569 552 320 49 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4241 4258 4411 4596 5041 325
Size: 1255 2661 17 153 185 445 1498
Fragments arranged by size:
2661 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2886 3132 4460
Size: 274 80 33 51 1443 909 246 1328
Cuts at: 4460 6172 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1328 909 274 246 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6174 6206 6174
Size: 32 6182
Tth111I GACn'n_nGTC
Cuts at: 1316 2599 1316
Size: 1283 4931
XbaI T'CTAG_A
Cuts at: 3550 3550
Size: 6214
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6214
XmaI C'CCGG_G
Cuts at: 1870 2741 3539 1870
Size: 871 798 4545
Fragments arranged by size:
4545 871 798
XmnI GAAnn'nnTTC
Cuts at: 2141 2655 4343 2141
Size: 514 1688 4012
Fragments arranged by size:
4012 1688 514
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4022 276
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PspXI PsrI RsrII SexAI SfoI SrfI
SwaI XcmI